Immunology

Direct contact between iPSC-derived macrophages and hepatocytes drives reciprocal acquisition of Kupffer cell identity and hepatocyte maturation

1. Christopher Zhe Wei Lee
2. Farah Tasnim
3. Xiaozhong Huang
4. Raman Sethi
5. Yoohyun Song
6. Tatsuya Kozaki
7. Sebastiaan De Schepper
8. Nicholas Ang
9. Ivy Low
10. You Yi Hwang
11. Jinmiao Chen
12. Hanry Yu
13. Florent Ginhoux

• Curated by eLife

  eLife Assessment

  This timely and fundamental study introduces a human iPSC-based co-culture system that models Kupffer cell-hepatocyte interactions and aims to recapitulate liver-specific immune-parenchymal dynamics. Direct contact between iMacs and iHeps promotes mutual tissue-specific maturation, with iHeps downregulating fetal genes while iMacs acquire a Kupffer cell-like profile. This convincing in vitro model holds significant promise and is a leap forward; future experimental understanding will enhance its translational impact.

Reviewed by eLife

A major histocompatibility complex (MHC) class II molecule that binds the same viral pathogen peptide with both nonamer and decamer core sequences for presentation to T cells

1. Anastasia Goryanin
2. Atlanta G. Cook
3. Shahriar Behboudi
4. Jim Kaufman
5. Samer Halabi

Reviewed by preLights

B cell expression of the enzyme PexRAP, an intermediary in ether lipid biosynthesis, promotes antibody responses and germinal center size

1. Sung Hoon Cho
2. Marissa A Jones
3. Kaylor Meyer
4. David M Anderson
5. Sergei Chetyrkin
6. M Wade Calcutt
7. Richard M Caprioli
8. Clay F Semenkovich
9. Mark R Boothby

• Curated by eLife

  eLife Assessment

  This study provides useful insights into the ways in which germinal center B cell metabolism, particularly lipid metabolism, affects cellular responses. The authors use sophisticated mouse models to convincingly demonstrate that ether lipids are relevant for B cell homeostasis and efficient humoral responses. The authors then conducted in vivo as well as in vitro experiments, thereby strengthening their conclusions.

Reviewed by eLife

Explosive cytotoxicity of ‘ruptoblasts’ bridges hormonal surveillance and immune defense

1. Chew Chai
2. Eliya Sultan
3. Souradeep R. Sarkar
4. Lihan Zhong
5. Dania Nanes Sarfati
6. Orly Gershoni-Yahalom
7. Christine Jacobs-Wagner
8. Hawa Racine Thiam
9. Benyamin Rosental
10. Bo Wang

Reviewed by preLights

RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

1. Ahmed Rishiq
2. Johanna Galski
3. Reem Bsoul
4. Mingdong Liu
5. Rema Darawshe
6. Renate Lux
7. Gilad Bachrach
8. Ofer Mandelboim

• Curated by eLife

  eLife Assessment

  This useful study describes a mechanism of microbial modulation of anti-tumor immunity, which is of considerable interest in the field. However, the experimental supports for the key mechanistic claim, the interaction between RadD and NKp46, are not robust. Multiple experimental inconsistencies, especially in vivo, weaken the conclusions, making the strength of evidence incomplete. Additional controls, direct binding assays, and clarification of in vivo mechanistic relevance would strengthen the work.

Reviewed by eLife

Pre-Omicron Immunity Generates IgG⁺ but Not IgA⁺ Memory B Cells Reactive to Omicron Spike Protein

1. T Tsoleridis
2. I Singh
3. D Onion
4. JG Chappell
5. A Kelly
6. J Nightingale
7. AM Valdes
8. BJ Ollivere
9. KB Hoehn
10. RA Urbanowicz
11. JK Ball

Reviewed by PREreview

Layers of immunity: Deconstructing the Drosophila effector response

1. Faustine Ryckebusch
2. Yao Tian
3. Mylene Rapin
4. Fanny Schüpfer
5. Mark Austin Hanson
6. Bruno Lemaitre

• Curated by eLife

  eLife Assessment

  This work provides one of the first important attempts to look at Drosophila immune responses against bacterial, viral, and fungal pathogens in a way that combines the roles of four major arms in immunity (Imd signaling, Toll signaling, phagocytosis, and melanization) rather than studying them separately. The findings are compelling and the tools provided can be used as they are, or built upon, in various contexts.

Reviewed by eLife

IRF2 degradation tunes the innate immune response

1. Cristhian Cadena
2. Rohit Reja
3. Emma Bolech
4. Joshua D. Webster
5. Vasumathi Kameswaran
6. Marco de Simone
7. Cynthia Chen
8. Jian Jiang
9. Kathy Hotzel
10. Kamela Alegre
11. Zhenyu Tan
12. Raymond Newland
13. Ryan Kelly
14. Spyros Darmanis
15. Bence Daniel
16. Ishan Deshpande
17. Kim Newton
18. Nobuhiko Kayagaki
19. Vishva M. Dixit

Reviewed by Arcadia Science

Clonal stochasticity in early NK cell response to mouse cytomegalovirus is generated by mature subsets of varying proliferative ability

1. Darren Wethington
2. Saeed Ahmad
3. Marc Potempa
4. Giuseppe Giuliani
5. Oscar A Aguilar
6. Maheshwor Poudel
7. Simon Grassmann
8. William Stewart
9. Nicholas M Adams
10. Joseph C Sun
11. Lewis L Lanier
12. Jayajit Das

• Curated by eLife

  eLife Assessment

  This study combines mathematical models and experimental data to analyse the emergence of heterogeneity within clonal NK cell responses during antigen-specific cell expansion. It comprises different experimental data and extensively explores various mathematical models, to study NK cell turnover during acute immune responses and homeostatic turnover within murine cytomegalovirus infection (MCMV). The solid study presents valuable findings and provides insights on heterogeneous NK cell development

Reviewed by eLife

Epidermal resident memory T cell fitness requires antigen encounter in the skin

1. Eric S Weiss
2. Toshiro Hirai
3. Haiyue Li
4. Andrew Liu
5. Shannon Baker
6. Ian Magill
7. Jacob Gillis
8. Youran R Zhang
9. Torben Ramcke
10. Kazuo Kurihara
11. The ImmGen Consortium OpenSource T cell Project
12. David Masopust
13. Niroshana Anandasabapathy
14. Harinder Singh
15. David Zemmour
16. Laura K Mackay
17. Daniel H Kaplan

• Curated by eLife

  eLife Assessment

  This manuscript advances the prior finding that antigen recognition in the skill helps establish skin resident memory in CD8 T cells by elucidating the role of TGFBR3 in regulating CD8+ TRM skin persistence upon topical antigen exposure. Key novelty of the your work lies in generation and use of the CD8+ T cell-specific TGFBR3 knockout model, which allows them to demonstrate the role of TGFBR3 in fine tuning the degree of CD8+ T cell skin persistence and that TGFBR3 expression is promoted by CD8+ TRM encountering their cognate antigen upon initial skin entry. This is an important finding and is supported by convincing evidence. There are concerns about the use of FTY720 and the need to establish active TGFbeta limiting conditions to further test this working model.

Reviewed by eLife