Immunology

Functional visualization of NK cell-mediated killing of metastatic single tumor cells

1. Hiroshi Ichise
2. Shoko Tsukamoto
3. Tsuyoshi Hirashima
4. Yoshinobu Konishi
5. Choji Oki
6. Shinya Tsukiji
7. Satoshi Iwano
8. Atsushi Miyawaki
9. Kenta Sumiyama
10. Kenta Terai
11. Michiyuki Matsuda

Reviewed by Review Commons

Expansion of CD10neg neutrophils and CD14+HLA-DRneg/low monocytes driving proinflammatory responses in patients with acute myocardial infarction

1. Daniela Fraccarollo
2. Jonas Neuser
3. Julian Möller
4. Christian Riehle
5. Paolo Galuppo
6. Johann Bauersachs

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to cardiologist and scientists studying acute myocardial infarction (AMI), especially to those focussing on the immune responses during AMI. Using combination of in vivo and in vitro model, as well as tissue from patients, the authors reveal new insights regarding the immune mechanisms during AMI, highlighting the importance of neutrophils and monocytes during the early days of its process. The findings in this paper add to the understanding of how immune mechanisms may contribute to subsequent adverse events after AMI.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors)

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Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells

1. Almut S Eisele
2. Jason Cosgrove
3. Aurelie Magniez
4. Emilie Tubeuf
5. Sabrina Tenreira Bento
6. Cecile Conrad
7. Fanny Cayrac
8. Tamar Tak
9. Anne-Marie Lyne
10. Jos Urbanus
11. Leïla Perié

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to readers in the field of cytokine signaling, experimental hematology and clinical hematology. Erythropoietin is one of the most widely used cytokines clinically but the cells it exerts its effects on has been debated. This study has combined clonal lineage tracing and single cell sequencing to understand the cell population that responds to erythropoietin and indicates that erythropoietin acts directly on multipotent progenitors to transiently modulate their output.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Systemic inflammation recruits fast-acting anti-inflammatory innate myeloid progenitors from BM into lymphatics

1. Juana Serrano-Lopez
2. Shailaja Hegde
3. Sachin Kumar
4. Josefina Serrano
5. Jing Fang
6. Ashley M. Wellendorf
7. Paul A. Roche
8. Yamileth Rangel
9. Léolène J. Carrington
10. Hartmut Geiger
11. H. Leighton Grimes
12. Sanjiv Luther
13. Ivan Maillard
14. Joaquin Sanchez-Garcia
15. Daniel T. Starczynowski
16. Jose A. Cancelas

• Curated by eLife

  Evaluation Summary:

  The authors demonstrate that acute systemic inflammation induces a new system of rapid migration of granulocyte-macrophage progenitors and committed macrophage-dendritic progenitors but not other progenitors or stem cells from BM to lymphatic capillaries. The cells appear in the lymphatics earlier than in peripheral blood. This type of trafficking is triggered by LPS administration and is anti-inflammatory.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Stepwise transmigration of T- and B cells through a perivascular channel in high endothelial venules

1. Kibaek Choe
2. Jieun Moon
3. Soo Yun Lee
4. Eunjoo Song
5. Ju Hee Back
6. Joo-Hye Song
7. Young-Min Hyun
8. Kenji Uchimura
9. Pilhan Kim

Reviewed by Review Commons

In vivo fluorescence lifetime imaging of macrophage intracellular metabolism during wound responses in zebrafish

1. Veronika Miskolci
2. Kelsey E Tweed
3. Michael R Lasarev
4. Emily C Britt
5. Alex J Walsh
6. Landon J Zimmerman
7. Courtney E McDougal
8. Mark R Cronan
9. Jing Fan
10. John-Demian Sauer
11. Melissa C Skala
12. Anna Huttenlocher

• Curated by eLife

  Evaluation Summary:

  Immunometabolism is an emerging field, and to understand immune cell metabolism during inflammation and infection is of great interest. In this report, cutting edge (label free) microscopy techniques and innovative zebrafish models are used to characterize the metabolism of macrophages in situ. In the future, fluorescence microscopy approaches pioneered using zebrafish may illuminate strategies to therapeutically manipulate metabolism in human immune cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

The Drosophila Baramicin polypeptide gene protects against fungal infection

1. Mark Austin Hanson
2. Lianne B. Cohen
3. Alice Marra
4. Igor Iatsenko
5. Steven A. Wasserman
6. Bruno Lemaitre

Reviewed by Review Commons

Bone marrow adipocytes drive the development of tissue invasive Ly6Chigh monocytes during obesity

1. Parastoo Boroumand
2. David C Prescott
3. Tapas Mukherjee
4. Philip J Bilan
5. Michael Wong
6. Jeff Shen
7. Ivan Tattoli
8. Yuhuan Zhou
9. Angela Li
10. Tharini Sivasubramaniyam
11. Nancy Shi
12. Lucie Y Zhu
13. Zhi Liu
14. Clinton Robbins
15. Dana J Philpott
16. Stephen E Girardin
17. Amira Klip

• Curated by eLife

  Evaluation Summary:

  Using mice fed with high fat diet (HFD), Boroumand et al. observed a link between bone marrow (BM) adipocyte whitening and the expansion of BM Ly6Chi monocytes and derived cells in the adipose tissue. By adopting an in vitro approach, they also show that BM conditioned medium is able to metabolically rewire Ly6Chi monocytes notably concerning mitochondrial fission/fusion gene expression. They conclude that early changes in the BM adipocytes induced by HFD drive the activation of monocytes and influence the outcome of the disease.

  This study is of interest to those investigating BM adaptations to lipid signals as well as macrophage biologists interested in macrophage recruitment and differentiation in the context of obesity and beyond in inflammation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors)

Reviewed by eLife

A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection

1. Tania Lebratti
2. Ying Shiang Lim
3. Adjoa Cofie
4. Prabhakar Andhey
5. Xiaoping Jiang
6. Jason Scott
7. Maria Rita Fabbrizi
8. Ayşe Naz Ozantürk
9. Christine Pham
10. Regina Clemens
11. Maxim Artyomov
12. Mary Dinauer
13. Haina Shin

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest to a broad audience of immunologists especially those studying host-pathogen interactions, mucosal immunology, innate immunity and interferons. The study reveals a novel role for neutrophils in the regulation of pathological inflammation during viral infection of the genital mucosa. The main conclusions are well supported by a combination of precise technical approaches including neutrophil-specific gene targeting and antibody-mediated inhibition of selected pathways.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

IL-33 promotes innate lymphoid cell-dependent IFN-γ production required for innate immunity to Toxoplasma gondii

1. Joseph T Clark
2. David A Christian
3. Jodi A Gullicksrud
4. Joseph A Perry
5. Jeongho Park
6. Maxime Jacquet
7. James C Tarrant
8. Enrico Radaelli
9. Jonathan Silver
10. Christopher A Hunter

• Curated by eLife

  Evaluation Summary:

  This study sheds new light on the function of an immune system protein termed interleukin (IL)-33 in response to parasite infection. The study provides information on alternative functions of this immune protein and details the path taken to achieve a beneficial immune response. This study is of interest to immunologists who deal with the host response to infection, particularly to parasites. Immunotherapies that enhance or inhibit IL-33 are in development. Understanding the role of this immune factor in a broad range of infections is important when considering future treatments that target this pathway.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife