Immunology

Fate mapping analysis reveals a novel murine dermal migratory Langerhans-like cell population

1. Jianpeng Sheng
2. Qi Chen
3. Xiaoting Wu
4. Yu Wen Dong
5. Johannes Mayer
6. Junlei Zhang
7. Lin Wang
8. Xueli Bai
9. Tingbo Liang
10. Yang Ho Sung
11. Wilson Wen Bin Goh
12. Franca Ronchese
13. Christiane Ruedl

• Curated by eLife

  Evaluation Summary:

  The present study uses innovative approaches to further our knowledge of skin immunobiology. The corresponding results explain the expression of langerin on two fractions of dermal DC (CD103+ and CD103-) observed several years ago. The demonstration that epidermal LC do not contribute to LN populations in the steady state is completely unexpected and raises important questions about the in vivo function of the LC-like subset unveiled in the present study.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

ImmuNet: a segmentation-free machine learning pipeline for immune landscape phenotyping in tumors by multiplex imaging

1. Shabaz Sultan
2. Mark A J Gorris
3. Evgenia Martynova
4. Lieke L van der Woude
5. Franka Buytenhuijs
6. Sandra van Wilpe
7. Kiek Verrijp
8. Carl G Figdor
9. I Jolanda M de Vries
10. Johannes Textor

Reviewed by Arcadia Science

A genetic screen in macrophages identifies new regulators of IFNγ-inducible MHCII that contribute to T cell activation

1. Michael C Kiritsy
2. Laurisa M Ankley
3. Justin Trombley
4. Gabrielle P Huizinga
5. Audrey E Lord
6. Pontus Orning
7. Roland Elling
8. Katherine A Fitzgerald
9. Andrew J Olive

Reviewed by eLife

Mitochondrial respiration contributes to the interferon gamma response in antigen-presenting cells

1. Michael C Kiritsy
2. Katelyn McCann
3. Daniel Mott
4. Steven M Holland
5. Samuel M Behar
6. Christopher M Sassetti
7. Andrew J Olive

Reviewed by eLife

Ex vivo observation of granulocyte activity during thrombus formation

1. Daria S. Morozova
2. Alexey A. Martyanov
3. Sergei I. Obydennyi
4. Julia-Jessica D. Korobkin
5. Alexey V. Sokolov
6. Ekaterina V. Shamova
7. Irina V. Gorudko
8. Anna L. Khoreva
9. Anna Shcherbina
10. Mikhail A. Panteleev
11. Anastasia N. Sveshnikova

• Curated by eLife

  Summary: Specifically, the proposed model system is valuable to study ex vivo the infiltration of the growing thrombus by granulocytes using fluorescent microscopy. In addition, this system has the potential to facilitate investigations on the role of granulocytes in thrombus growth and immune-thrombosis. Granulocytes have been classified into two different types based on their DiOC6 staining pattern, namely, type A with uniform DiOC6 and type B with cluster-like DiOC6. However, it remains unclear if the staining pattern is homogeneously so clear-cut and if the type A and B granulocytes are in addition defined by their velocity. Granulocyte activation process by "priming agents" has to be validated and the rationale for using the chosen agents needs to be provided. Finally, better-defined controls for the part of the paper dedicated to the synergistic effect between granulocytes and platelets during thrombus growth are necessary. Because in the Wiskott-Aldrich syndrome (WAS) granulocyte motility as well as platelet number and function are impaired, blood from patients with WAS is not an appropriate control for this study. For example, for the control experiments, the following controls might be used in replacement of WAS blood: (1) blood from thrombocytopenic patients or platelet-depleted blood and (2) blood in which granulocyte mobility/activation is inhibited. Finally, it would be interesting to see if neutrophil extracellular traps (NETs) develop in this model system.

Reviewed by eLife

IL-13 deficiency exacerbates lung damage and impairs epithelial-derived type 2 molecules during nematode infection

1. Alistair L Chenery
2. Silvia Rosini
3. James E Parkinson
4. Jesuthas Ajendra
5. Jeremy A Herrera
6. Craig Lawless
7. Brian HK Chan
8. P’ng Loke
9. Andrew S MacDonald
10. Karl E Kadler
11. Tara E Sutherland
12. Judith E Allen

Reviewed by Review Commons

Susceptibility rhythm to bacterial endotoxin in myeloid clock-knockout mice

1. Veronika Lang
2. Sebastian Ferencik
3. Bharath Ananthasubramaniam
4. Achim Kramer
5. Bert Maier

Reviewed by eLife

Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node

1. Shannon M Walsh
2. Ryan M Sheridan
3. Erin D Lucas
4. Thu A Doan
5. Brian C Ware
6. Johnathon Schafer
7. Rui Fu
8. Matthew A Burchill
9. Jay R Hesselberth
10. Beth Ann Jiron Tamburini

Reviewed by eLife

Inflammation drives alternative first exon usage to regulate immune genes including a novel iron-regulated isoform of Aim2

1. Elektra K Robinson
2. Pratibha Jagannatha
3. Sergio Covarrubias
4. Matthew Cattle
5. Valeriya Smaliy
6. Rojin Safavi
7. Barbara Shapleigh
8. Robin Abu-Shumays
9. Miten Jain
10. Suzanne M Cloonan
11. Mark Akeson
12. Angela N Brooks
13. Susan Carpenter

Reviewed by eLife

Machine Learning Analysis of the Bleomycin Mouse Model Reveals the Compartmental and Temporal Inflammatory Pulmonary Fingerprint

1. Natalie Bordag
2. Valentina Biasin
3. Diana Schnoegl
4. Francesco Valzano
5. Katharina Jandl
6. Bence M. Nagy
7. Neha Sharma
8. Malgorzata Wygrecka
9. Grazyna Kwapiszewska
10. Leigh M. Marsh

Reviewed by eLife