1. Chromosome-Specific Aneuploidy Engineering via dCas9-Induced Centromeric Chromatin Relaxation

    This article has 39 authors:
    1. Hugang Feng
    2. Daqi Deng
    3. Rashmi Dahiya
    4. Libin Wang
    5. Jingkun Zeng
    6. Benjy Jek Yang Tan
    7. Fiona Byrne
    8. Scott T. C. Shepherd
    9. Jiahao Wang
    10. Sarah C. Johnson
    11. Alison Harrod
    12. Karen A. Lane
    13. Annika Fendler
    14. Anne-Laure Cattin
    15. Zayd Tippu
    16. Meilun Nie
    17. Yiming Zhao
    18. Ruijia Wang
    19. Wei Ai
    20. Omar Bouricha
    21. Taja Barber
    22. Yuliia Dovga
    23. Yihan Xu
    24. Yimeng Xu
    25. Liani G. Devito
    26. Lyn Healy
    27. Zhen Sun
    28. Weiming Shen
    29. Hongye Wang
    30. Eugénie S. Lim
    31. Samuel M. O’Toole
    32. Scott Akker
    33. William M. Drake
    34. Haojie Jin
    35. Jessica A. Downs
    36. Sarah E. McClelland
    37. John F. X. Diffley
    38. Peter Ly
    39. Samra Turajlic

    Reviewed by PREreview

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  2. Systematic analysis of transcription factor combinatorial binding uncovers TEAD1 as an antagonist of tissue-specific transcription factors in human organogenesis

    This article has 6 authors:
    1. Araceli Garcia-Mora
    2. Joshua Mallen
    3. Peyman Zarrineh
    4. Neil Hanley
    5. Dave Gerrard
    6. Nicoletta Bobola
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study presents a pipeline for discovering cooperative transcription factor (TF) interactions that regulate development, and applies this pipeline in a systematic investigation of TF co-regulation in 11 human embryonic tissues. The authors provide overall solid bioinformatics and experimental support for their findings: although they make a convincing argument for the role of TEAD factors as co-repressors of regulatory activity with tissue-specific TFs, other aspects of the study would benefit from additional validation. This work would be of interest to cell biologists focused on development or on discovery of TF relationships.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  3. The first near-complete genome assembly of pig: enabling more accurate genetic research

    This article has 13 authors:
    1. Caiyun Cao
    2. Jian Miao
    3. Qinqin Xie
    4. Jiabao Sun
    5. Hong Cheng
    6. Zhenyang Zhang
    7. Fen Wu
    8. Shuang Liu
    9. Xiaowei Ye
    10. Zhe Zhang
    11. Qishan Wang
    12. Yuchun Pan
    13. Zhen Wang

    Reviewed by GigaScience

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  4. Genome organization by SATB1 binding to base-unpairing regions (BURs) provides a scaffold for SATB1-regulated gene expression

    This article has 13 authors:
    1. Yoshinori Kohwi
    2. Xianrong Wong
    3. Mari Grange
    4. Thomas Sexton
    5. Hunter W Richards
    6. Yohko Kitagawa
    7. Shimon Sakaguchi
    8. Ya-Chen Liang
    9. Cheng-Ming Chuong
    10. Vladimir A Botchkarev
    11. Ichiro Taniuchi
    12. Karen L Reddy
    13. Terumi Kohwi-Shigematsu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This is a very important study in which the authors have modified ChIP-seq and 4C-seq with a urea step, which drastically changes the pattern of chromatin interactions observed for SATB1, but not other proteins (including CTCF). The study highlights that the urea protocols provide a complementary view of protein-chromatin interactions for some proteins, which can uncover previously hidden, functionally significant layers of chromatin organization. If applied more widely, these protocols may significantly further our understanding of chromatin organization. The study's findings are supported by a wealth of controls, making the evidence compelling.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  5. Gene expression variation across genetically identical individuals predicts reproductive traits

    This article has 5 authors:
    1. Amy K. Webster
    2. John H. Willis
    3. Erik Johnson
    4. Peter Sarkies
    5. Patrick C. Phillips
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Complex traits are influenced by genes and the environment, but especially the latter is difficult to pin down. This important study uses C. elegans to demonstrate that non-genetic differences in gene expression, partly influenced by the environment, correlate with individual differences in two reproductive traits. This supports the use of gene expression data as a key intermediate for understanding complex traits. The clever study design makes for compelling evidence, which is further strengthened by experimental confirmation that identified differentially expressed genes indeed influence these traits.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. Exploiting functional regions in the viral RNA genome as druggable entities

    This article has 10 authors:
    1. Dehua Luo
    2. Yingge Zheng
    3. Zhiyuan Huang
    4. Zi Wen
    5. Lijun Guo
    6. Yingxiang Deng
    7. Qingling Li
    8. Yuqing Bai
    9. Shozeb Haider
    10. Dengguo Wei
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The study presents a fundamental advance in antiviral RNA research by adapting SHAPE-Map to chart the secondary structure of the Porcine Epidemic Diarrhoea Virus (PEDV) genome in infected cells and pinpointing structurally conserved, accessible RNA elements as therapeutic targets. A broad, well-documented integration of biochemical probing, computational analysis, and functional validation provides convincing evidence that these regions are both biologically relevant and druggable. Beyond PEDV, the work offers a generalizable framework for RNA-guided antiviral discovery that will interest researchers in RNA therapeutics and viral genome biology.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  7. Chromosome-level genome assemblies of five Sinocyclocheilus species

    This article has 6 authors:
    1. Chao Bian
    2. Ruihan Li
    3. Yuqian Ouyang
    4. Junxing Yang
    5. Xidong Mu
    6. Qiong Shi
    This article has been curated by 1 group:
    • Curated by GigaByte

      **Editors Assessment: ** Sinocyclocheilus are a genus of freshwater cavefish fish that are endemic to the Karst regions of Southwest China. Having diverse traits in morphology, behavior, and physiology typical of cavefish, that make them interesting models for studying cave adaptation and phylogenetic evolution. The manuscript assembled chromosomal-level genomes of five Sinocyclocheilus species, and conducted allotetraploid origin analysis on these species. Assembling S. grahami (the golden-line barbel), using PacBio and Hi-C sequencing technologies, a final chromosome-level genome assembly was 1.6 Gb in size with a contig N50 of 738.5 kb and a scaffold N50 of 30.7 Mb. With 93.1% of the assembled genome sequences and 93.8% of the predicted genes anchored onto 48 chromosomes. Subsequently the authors conducted a homologous comparison to obtain chromosome-level genome assemblies for four other Sinocyclocheilus species: S. maitianheensis, S. rhinocerous, S. anshuiensis, and S. Anophthalmus. With over 82% of the genome sequences anchored on these constructed chromosomes. Peer review provided clarification on the assembly strategy and provided more benchmarking. This data having the potential to contribute to species conservation and the exploitation of potential economic and ecological values of diverse Sinocyclocheilus members.

      This evaluation refers to version 1 of the preprint

    Reviewed by GigaByte

    This article has 2 evaluationsAppears in 2 listsLatest version Latest activity
  8. A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients

    This article has 68 authors:
    1. Yered Pita-Juarez
    2. Dimitra Karagkouni
    3. Nikolaos Kalavros
    4. Johannes C. Melms
    5. Sebastian Niezen
    6. Toni M. Delorey
    7. Adam L. Essene
    8. Olga R. Brook
    9. Deepti Pant
    10. Disha Skelton-Badlani
    11. Pourya Naderi
    12. Pinzhu Huang
    13. Liuliu Pan
    14. Tyler Hether
    15. Tallulah S. Andrews
    16. Carly G. K. Ziegler
    17. Jason Reeves
    18. Andriy Myloserdnyy
    19. Rachel Chen
    20. Andy Nam
    21. Stefan Phelan
    22. Yan Liang
    23. Mark Gregory
    24. Shanshan He
    25. Michael Patrick
    26. Tushar Rane
    27. Aster Wardhani
    28. Amit Dipak Amin
    29. Jana Biermann
    30. Hanina Hibshoosh
    31. Molly Veregge
    32. Zachary Kramer
    33. Christopher Jacobs
    34. Yusuf Yalcin
    35. Devan Phillips
    36. Michal Slyper
    37. Ayshwarya Subramanian
    38. Orr Ashenberg
    39. Zohar Bloom-Ackermann
    40. Victoria M. Tran
    41. James Gomez
    42. Alexander Sturm
    43. Shuting Zhang
    44. Stephen J. Fleming
    45. Sarah Warren
    46. Joseph Beechem
    47. Deborah Hung
    48. Mehrtash Babadi
    49. Robert F. Padera
    50. Sonya A. MacParland
    51. Gary D. Bader
    52. Nasser Imad
    53. Isaac H. Solomon
    54. Eric Miller
    55. Stefan Riedel
    56. Caroline B. M. Porter
    57. Alexandra-Chloé Villani
    58. Linus T.-Y. Tsai
    59. Winston Hide
    60. Gyongyi Szabo
    61. Jonathan Hecht
    62. Orit Rozenblatt-Rosen
    63. Alex K. Shalek
    64. Benjamin Izar
    65. Aviv Regev
    66. Yury V. Popov
    67. Z. Gordon Jiang
    68. Ioannis S. Vlachos

    Reviewed by Rapid Reviews Infectious Diseases

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  9. Post-fertilization transcription initiation in an ancestral LTR retrotransposon drives lineage-specific genomic imprinting of ZDBF2

    This article has 22 authors:
    1. Hisato Kobayashi
    2. Tatsushi Igaki
    3. Soichiro Kumamoto
    4. Keisuke Tanaka
    5. Tomoya Takashima
    6. So I Nagaoka
    7. Shunsuke Suzuki
    8. Masaaki Hayashi
    9. Marilyn B Renfree
    10. Manabu Kawahara
    11. Shun Saito
    12. Toshihiro Kobayashi
    13. Hiroshi Nagashima
    14. Hitomi Matsunari
    15. Kazuaki Nakano
    16. Ayuko Uchikura
    17. Hiroshi Kiyonari
    18. Mari Kaneko
    19. Hiroo Imai
    20. Kazuhiko Nakabayashi
    21. Matthew Lorincz
    22. Kazuki Kurimoto
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The authors analyses describe a novel mechanism by which a retrotransposon-derived LTR may be involved in genomic imprinting and demonstrate imprinting of the ZDBF2 locus in rabbits and Rhesus macaques using allele-specific expression analysis. This imprinting of the ZDBF2 locus correlates with transcription of GPR1-AS orthologs. The accompanying genomic analysis is very well executed allowing for the conclusions reached in the manuscript. The revisions made at the request of the reviewers in this important manuscript strengthen the evidence from the genomic analyses, and as a result, the evidence is now convincing and will be informative to the genomics and developmental biology communities.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  10. OTX2 controls chromatin accessibility to direct somatic versus germline differentiation

    This article has 2 authors:
    1. Elisa Barbieri
    2. Ian Chambers

    Reviewed by Review Commons

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
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