Genetics

Systematic investigation of imprinted gene expression and enrichment in the mouse brain explored at single-cell resolution

1. M. J. Higgs
2. M. J. Hill
3. R. M. John
4. A. R. Isles

• Curated by eLife

  Summary: The reviewers appreciated the effort to merge most of the available datasets to make a precise survey of the sites of imprinted gene expression and the great resource it could bring to the community. However, the reviewers also felt that the study suffered from methodological bias, was preliminary (no allelic information in particular), and that the conclusions did not go beyond previous reports. The general lack of citation of the name of imprinted genes made it difficult to judge whether conclusions were consistent among the different datasets. Highlighting specific imprinted genes would bring a clearer focus and narrative.

Reviewed by eLife

A CRISPR/Cas9 vector system for neutrophil-specific gene disruption in zebrafish

1. Yueyang Wang
2. Alan Y. Hsu
3. Eric M. Walton
4. Ramizah Syahirah
5. Tianqi Wang
6. Wenqing Zhou
7. Chang Ding
8. Abby Pei Lemke
9. David M. Tobin
10. Qing Deng

Reviewed by eLife

Paternal multigenerational exposure to an obesogenic diet drives epigenetic predisposition to metabolic disorders

1. Georges Raad
2. Fabrizio Serra
3. Luc Martin
4. Marie-Alix Derieppe
5. Jérôme Gilleron
6. Vera L Costa
7. Didier F Pisani
8. Ez-Zoubir Amri
9. Michele Trabucchi
10. Valérie Grandjean

Reviewed by eLife

Fluorescent in vivo editing reporter (FIVER): A novel multispectral reporter of in vivo genome editing

1. Peter A. Tennant
2. Robert G. Foster
3. Daniel O. Dodd
4. Ieng Fong Sou
5. Fraser McPhie
6. Nicholas Younger
7. Laura C. Murphy
8. Matthew Pearson
9. Bertrand Vernay
10. Margaret A. Keighren
11. Peter Budd
12. Stephen L. Hart
13. Roly Megaw
14. Luke Boulter
15. Pleasantine Mill

Reviewed by eLife

A catalog of homoplasmic and heteroplasmic mitochondrial DNA variants in humans

1. Alexandre Bolze
2. Fernando Mendez
3. Simon White
4. Francisco Tanudjaja
5. Magnus Isaksson
6. Ruomu Jiang
7. Andrew Dei Rossi
8. Elizabeth T. Cirulli
9. Misha Rashkin
10. William J. Metcalf
11. Joseph J. Grzymski
12. William Lee
13. James T. Lu
14. Nicole L. Washington

Reviewed by eLife

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes

1. François Kroll
2. Gareth T Powell
3. Marcus Ghosh
4. Paride Antinucci
5. Timothy J Hearn
6. Hande Tunbak
7. Sumi Lim
8. Harvey W Dennis
9. Joseph M Fernandez
10. David Whitmore
11. Elena Dreosti
12. Stephen W Wilson
13. Ellen J Hoffman
14. Jason Rihel

Reviewed by eLife

A missense in HSF2BP causing Primary Ovarian Insufficiency affects meiotic recombination by its novel interactor C19ORF57/MIDAP

1. Natalia Felipe-Medina
2. Sandrine Caburet
3. Fernando Sánchez-Sáez
4. Yazmine B. Condezo
5. Dirk de Rooij
6. Laura Gómez-H
7. Rodrigo García-Valiente
8. Anne-Laure Todeschini
9. Paloma Duque
10. Manuel Sánchez-Martín
11. Stavit A. Shalev
12. Elena Llano
13. Reiner Veitia
14. Alberto M. Pendás

Reviewed by eLife

Interpreting the pathogenicity of Joubert Syndrome missense variants in Caenorhabditis elegans

1. Karen I. Lange
2. Sofia Tsiropoulou
3. Katarzyna Kucharska
4. Oliver E. Blacque

Reviewed by eLife

Dominant negative effects on H3K27 methylation by Weaver syndrome-associated EZH2 variants

1. Orla Deevy
2. Craig Monger
3. Francesca Matrà
4. Ellen Tuck
5. Eric Conway
6. Mihaly Badonyi
7. Darragh Nimmo
8. Simona Rodighiero
9. Qi Zhang
10. Chen Davidovich
11. Joseph A. Marsh
12. Diego Pasini
13. Adrian P. Bracken

Reviewed by PREreview

Regulation of defective mitochondrial DNA accumulation and transmission in C. elegans by the programmed cell death and aging pathways

1. Sagen Flowers
2. Rushali Kothari
3. Yamila N Torres Cleuren
4. Melissa R Alcorn
5. Chee Kiang Ewe
6. Geneva Alok
7. Samantha L Fiallo
8. Pradeep M Joshi
9. Joel H Rothman

• Curated by eLife

  eLife assessment

  This manuscript suggests a novel mechanism of purifying selection by which programmed cell death contributes to the selective removal of mtDNA deletion mutations in C. elegans. The evidence for this mechanism of removal is strong although questions remain regarding the underlying mechanism and the role of canonical ageing pathways. Because of the likely central role of mtDNA deletions in ageing and age-dependent diseases, this work will be of interest to scientists in the field of mitochondrial biology as well as ageing.

Reviewed by eLife