Genetics

Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genes

1. Lee-Ann Notice-Sarpaning
2. Mathieu Catala
3. Sherif Abou Elela
4. Ambro van Hoof

• Curated by eLife

  eLife Assessment

  This valuable study expands the inventory of polyadenylated RNAs cleaved by the double-stranded RNA endonuclease Rnt1 in budding yeast, using solid methodology based on high-throughput sequencing. Previous studies had anecdotally discovered mRNA substrates, and this global characterization is comprehensive with multiple complementary controls. However, the study would be stronger with a deeper investigation into the biological function of Rnt1, as well as experiments directly probing the interaction between Rnt1 and its putative substrates.

Reviewed by eLife

Direct cell-to-cell transmission of retrotransposons

1. Maya Voichek
2. Andreas Bernhard
3. Maria Novatchkova
4. Dominik Handler
5. Paul Möseneder
6. Baptiste Rafanel
7. Peter Duchek
8. Kirsten-André Senti
9. Julius Brennecke

Reviewed by preLights

The robust, high-throughput, and temporally regulated roxCre and loxCre reporting systems for genetic modifications in vivo

1. Mengyang Shi
2. Jie Li
3. Xiuxiu Liu
4. Kuo Liu
5. Lingjuan He
6. Wenjuan Pu
7. Wendong Weng
8. Shaohua Zhang
9. Huan Zhao
10. Kathy O Lui
11. Bin Zhou

• Curated by eLife

  eLife Assessment

  This study presents an important set of new tools to facilitate Cre or Dre-mediated recombination in mice. The characterization of these new tools was done using solid and validated methodology. The work convincingly demonstrates the efficient gene knockout capability of these models and will progress the field.

Reviewed by eLife

Inferring genotype-phenotype maps using attention models

1. Krishna Rijal
2. Caroline M. Holmes
3. Samantha Petti
4. Gautam Reddy
5. Michael M. Desai
6. Pankaj Mehta

Reviewed by Arcadia Science

EPHA4 signaling dysregulation links abnormal locomotion and the development of idiopathic scoliosis

1. Lianlei Wang
2. Xinyu Yang
3. Sen Zhao
4. Pengfei Zheng
5. Wen Wen
6. Kexin Xu
7. Xi Cheng
8. Qing Li
9. Anas M Khanshour
10. Yoshinao Koike
11. Junjun Liu
12. Xin Fan
13. Nao Otomo
14. Zefu Chen
15. Yaqi Li
16. Lulu Li
17. Haibo Xie
18. Panpan Zhu
19. Xiaoxin Li
20. Yuchen Niu
21. Shengru Wang
22. Sen Liu
23. Suomao Yuan
24. Chikashi Terao
25. Ziquan Li
26. Shaoke Chen
27. Xiuli Zhao
28. Pengfei Liu
29. Jennifer E Posey
30. Zhihong Wu
31. Guixing Qiu
32. DISCO study group (Deciphering Disorders Involving Scoliosis & COmorbidities)
33. Shiro Ikegawa
34. James R Lupski
35. Jonathan J Rios
36. Carol A Wise
37. Terry Jianguo Zhang
38. Chengtian Zhao
39. Nan Wu

• Curated by eLife

  eLife Assessment

  Genetic variants have been strongly implicated in idiopathic scoliosis (IS), however, the list of variants that are causative of IS is not complete and the cellular and molecular mechanisms that underlie IS are poorly understood. These authors combined human genetic analysis with zebrafish experiments to produce valuable evidence that alleles that impair function of EPHA4 cause IS, thereby extending our understanding of the basis of IS. The human genetic data are quite convincing but the zebrafish work lacks some validations and details.

Reviewed by eLife

Condensin I ^DC has a Functional ATPase That is Required for X-Chromosome Dosage Compensation in C. elegans

1. Bahaar Chawla
2. Suchi Jatia
3. Dillon E. Sloan
4. Gyorgyi Csankovszki

Reviewed by Review Commons

Tissue-specific responses to TFAM and mtDNA copy number manipulation in prematurely ageing mice

1. Laura S Kremer
2. Guanbin Gao
3. Giovanni Rigoni
4. Roberta Filograna
5. Mara Mennuni
6. Rolf Wibom
7. Ákos Végvári
8. Camilla Koolmeister
9. Nils-Göran Larsson

• Curated by eLife

  eLife Assessment

  This is an important study that examines the role of TFAM, a protein that helps maintain mtDNA, in mtDNA mutator mice. With convincing evidence, the authors have demonstrated that TFAM's counteractive role in mtDNA mutator mice is tissue-specific. The study does a thorough job of assessing the impact of modulating TFAM levels in a polg mutator mouse model of aging. The authors have thoroughly addressed all the points raised during the first round of review.

Reviewed by eLife

Integrating bulk and single cell RNA-seq refines transcriptomic profiles of individual C. elegans neurons

1. Alec Barrett
2. Erdem Varol
3. Alexis Weinreb
4. Seth R Taylor
5. Rebecca M McWhirter
6. Cyril Cros
7. Berta Vidal
8. Manasa Basaravaju
9. Abigail Poff
10. John A Tipps
11. Maryam Majeed
12. Chen Wang
13. Emily A Bayer
14. Molly Reilly
15. Eviatar Yemini
16. HaoSheng Sun
17. Oliver Hobert
18. David M Miller
19. Marc Hammarlund

• Curated by eLife

  eLife Assessment

  This valuable paper provides refined gene expression datasets for 52 neuron classes in C. elegans using a new method that takes advantage of the complementary strengths of bulk sequencing of flow-sorted cells and single-cell sequencing. In general, support for the paper's findings is convincing. However, more rigorous consideration of some of the method's statistical assumptions and validation of the predicted gene sets would improve the work.

Reviewed by eLife

PWWP-ADD and N-terminal domains of DNMT3B1 confer specificity for developmentally regulated CpG island methylation

1. Komal Yasmin
2. Tatyana B Nesterova
3. Neil Brockdorff

Reviewed by Review Commons

TRPγ regulates lipid metabolism through Dh44 neuroendocrine cells

1. Dharmendra Kumar Nath
2. Subash Dhakal
3. Youngseok Lee

• Curated by eLife

  eLife Assessment

  This important study reports findings that Trpγ, a type of transient receptor potential (TRP) channel expressed in Dh44-releasing neuroendocrine cells, mediates starvation-dependent lipid catabolism. Overall, the claims of the authors are supported by solid evidence. The work should be of interest to both basic and medical biologists working on lipid metabolism.

Reviewed by eLife