Genetics

Mettl5 coordinates protein production and degradation of PERIOD to regulate sleep in Drosophila

1. Xiaoyu Wu
2. Xingzhuo Yang
3. Tiantian Fu
4. Yikang Rong
5. Juan Du

• Curated by eLife

  eLife Assessment

  The authors present useful findings demonstrating that the RNA modification enzyme Mettl5 regulates sleep in Drosophila. Through transcriptome- and proteome-wide analyses, the authors identified downstream targets affected in heterozygous mutants and proposed that Mettl5 regulates the translation and degradation of clock genes to maintain normal sleep function. Through additional analyses, the authors provided solid evidence supporting this model.

Reviewed by eLife

Endogenous corazonin signaling modulates the post-mating switch in behavior and physiology in females of the brown planthopper and Drosophila

1. Ning Zhang
2. Shao-Cong Su
3. Ruo-Tong Bu
4. Yi-Jie Zhang
5. Lei Yang
6. Jie Chen
7. Dick R Nässel
8. Cong-Fen Gao
9. Shun-Fan Wu

• Curated by eLife

  eLife Assessment

  This important study presents convincing evidence that uncovers a novel signaling axis impacting the post-mating response in females of the brown planthopper. The findings open several avenues for testing the molecular and neurobiological mechanisms of mating behavior in insects, and in the revised version the authors provide further evidence supporting their conclusions.

Reviewed by eLife

Effects of knockdown of autophagy pathway genes on C. elegans longevity are highly condition dependent

1. Kuei Ching Hsiung
2. Hannah Chapman
3. Xiaoya Wei
4. Xiaoyu Sun
5. Isadora Rawlinson
6. David Gems

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on the condition dependence of autophagy-mediated lifespan regulation in C. elegans. The evidence is solid, as the data broadly support the main claims, although variability between biological replicates and limited mechanistic exploration leave some conclusions less firmly established. The work will be of interest to researchers studying autophagy, ageing, and intracellular trafficking.

Reviewed by eLife

Drosophila melanogaster model of RVCL-S demonstrates age dependent disease progression

1. Elena Gracheva
2. Abigail Matt
3. Fei Wang
4. Raymond Hsin
5. Hongwu Liang
6. Xiangping Ouyang
7. Jimin Ding
8. Jonathan J Miner
9. Chao Zhou

• Curated by eLife

  eLife Assessment

  This manuscript describing the phenotypes associated with loss and gain of RVCL-S documents important findings that have practical implications. Although the data and methods are solid and support many claims, there remain some concerns about mechanisms.

Reviewed by eLife

Sickle cell status skews malaria parasite genotype at infection

1. Helena D. Hopson
2. Alejandra Herbert-Mainero
3. Gaelle Bouopda
4. Charlène Tina Nanssong-Vomo
5. Brigitte Tumamo
6. Belinda Kiam
7. Ibrahima Ibrahima
8. Clément Onguene
9. Luc Abate
10. Radoslaw Igor Omelianczyk
11. Heather D. Evans
12. Lyra E. Horton
13. Gavin Band
14. Tracey J. Lamb
15. Lawrence S. Ayong
16. Sandrine E. Nsango
17. Ellen M. Leffler

Reviewed by Rapid Reviews Infectious Diseases

Visible traits demonstrate that crispant founder mice can be used for phenotypic assessment

1. Rebekah Tillotson
2. Marina Gertsenstein
3. Li-Hsin Chang
4. Julie Ruston
5. Fernando Bellido Molías
6. Lauri G Lintott
7. Christine Taylor
8. Philippe Gautier
9. Lauryl MJ Nutter
10. Monica J Justice

• Curated by eLife

  eLife Assessment

  This study offers important methodological advances for CRISPR-based mutagenesis in mice, highlighting the potential of founder animals for early phenotypic characterization. The authors present convincing evidence, supported by rigorous experimental design, that "crispant" (F0) analysis in mice, despite prior concerns about genetic mosaicism, can be utilized to assess protein function.

Reviewed by eLife

Distinct chromatin regulators downmodulate meiotic axis protein deposition and DNA break induction at chromosome ends

1. Adhithi R Raghavan
2. Kieron May
3. Vijayalakshmi V Subramanian
4. Hannah G Blitzblau
5. Neem J Patel
6. Jonathan Houseley
7. Andreas Hochwagen

• Curated by eLife

  eLife Assessment

  This valuable paper describes the regulation of the association of meiotic chromosome axis proteins on chromosome ends with sub-telomeric elements in budding yeast. The genome-wide analyses of binding of chromosome components as well as chromatin regulators, complemented with the mapping of meiotic DNA double-strand breaks on chromosome ends, provided incomplete evidence to support the authors' conclusion. The results in the paper are of interest to researchers in meiotic recombination and the structure of genomes and chromosomes.

Reviewed by eLife

Functional Profiling of 2,193 ASS1 Missense Variants: Insights into Variant Pathogenicity and Epistatic Interactions in Citrullinemia Type I

1. Russell S. Lo
2. Gareth A. Cromie
3. Michelle Tang
4. Amy Sirr
5. Ljubica Caldovic
6. Hiroki Morizono
7. Nicholas Ah Mew
8. Andrea Gropman
9. Aimée M. Dudley

Reviewed by Review Commons