1. TRPγ regulates lipid metabolism through Dh44 neuroendocrine cells

    This article has 3 authors:
    1. Dharmendra Kumar Nath
    2. Subash Dhakal
    3. Youngseok Lee
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study using Drosophila genetics explores the role of TRPγ in Dh44 neuroendocrine cells for lipid and protein metabolism. Evidence for lipid storage and metabolism measured by triacylglycerol levels, lipid droplet size, and starvation resistance are generally solid to support the conclusion. However, the claim on the TRPγ functions in Dh44R2 is still unclear, as the analysis of the role and expression of Dh44R2 in the gut is incomplete.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. Deficiency of IQCH causes male infertility in humans and mice

    This article has 10 authors:
    1. Tiechao Ruan
    2. Ruixi Zhou
    3. Yihong Yang
    4. Junchen Guo
    5. Chuan Jiang
    6. Xiang Wang
    7. Gan Shen
    8. Siyu Dai
    9. Suren Chen
    10. Ying Shen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study describes mice with a knock out of the IQ motif-containing H (IQCH) gene, to model a human loss-of-function mutation in IQCH associated with male sterility. While the evidence for interaction between IQCH and potential RNA binding proteins is limited, the human infertility is reproduced in the mouse, making it a compelling model. The paper could be of interest to cell biologists and male reproductive biologists working on the sperm flagellar cytoskeleton and mitochondrial structure.

    Reviewed by eLife

    This article has 19 evaluationsAppears in 1 listLatest version Latest activity
  3. Target-specific requirements for RNA interference can arise through restricted RNA amplification despite the lack of specialized pathways

    This article has 5 authors:
    1. Daphne R Knudsen-Palmer
    2. Pravrutha Raman
    3. Farida Ettefa
    4. Laura De Ravin
    5. Antony M Jose
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study shows how an intersecting network of regulators acting on genes with differences in their RNA metabolism explains why the loss of some regulators of RNAi in C. elegans can selectively impair the silencing of some target genes. The evidence presented is convincing, as the authors use a combination of computational modeling and RNAi assays to support their conclusions.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  4. Evolutionary adaptation of an HP1-protein chromodomain integrates chromatin and DNA sequence signals

    This article has 7 authors:
    1. Lisa Baumgartner
    2. Jonathan J Ipsaro
    3. Ulrich Hohmann
    4. Dominik Handler
    5. Alexander Schleiffer
    6. Peter Duchek
    7. Julius Brennecke
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental work has completed our understanding of the singular binding profile of the Rhino HP1 protein to chromatin, a key step in converting certain genomic regions into piRNA source loci. The evidence supporting the conclusions is compelling. Phylogenetic analyses, structure prediction, rigorous biochemical assays and in vivo genetics emphasize the importance of the Rhino chromodomain in the recognition of both a histone mark and a DNA-binding protein, and highlight the importance of a single chromodomain residue in the protein-protein interaction.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  5. The retrotransposon - derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity

    This article has 21 authors:
    1. Thomas W.P. Wood
    2. William S. Henriques
    3. Harrison B. Cullen
    4. Mayra Romero
    5. Cecilia S. Blengini
    6. Shreya Sarathy
    7. Julia Sorkin
    8. Hilina Bekele
    9. Chen Jin
    10. Seungsoo Kim
    11. Alexei Chemiakine
    12. Rishad C. Khondker
    13. José V.V. Isola
    14. Michael B. Stout
    15. Vincenzo A. Gennarino
    16. Binyam Mogessie
    17. Devanshi Jain
    18. Karen Schindler
    19. Yousin Suh
    20. Blake Wiedenheft
    21. Luke E. Berchowitz

    Reviewed by Arcadia Science

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  6. Insights into Immune Gene Prediction and Function Through the Evolutionary History of ADF Gene Family

    This article has 2 authors:
    1. Huan Chen
    2. Brad Day

    Reviewed by Arcadia Science

    This article has 11 evaluationsAppears in 1 listLatest version Latest activity
  7. Caspar determines primordial germ cell identity in Drosophila melanogaster

    This article has 7 authors:
    1. Subhradip Das
    2. Sushmitha Hegde
    3. Neel Wagh
    4. Jyothish Sudhakaran
    5. Adheena Elsa Roy
    6. Girish Deshpande
    7. Girish S Ratnaparkhi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study investigates the role of Caspar (Casp), an orthologue of human Fas-associated factor-1, in regulating the number of primordial germ cells that form during Drosophila embryogenesis. The findings are important in that they reveal an additional pathway involved in germ cell specification and maintenance. The evidence supporting the conclusions is solid, as the authors identify Casp and its binding partner Transitional endoplasmic reticulum 94 (TER94) as factors that influence germ cell numbers. Minor changes to the title, text, and experimental design are recommended.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Chromosome Structure II: Stem-loops and Circle-loops

    This article has 4 authors:
    1. Wenfan Ke
    2. Miki Fujioka
    3. Paul Schedl
    4. James B. Jaynes
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable work investigates the role of boundary elements in the formation of 3D genome architecture. The authors established a specific model system that allowed them to manipulate boundary elements and examine the resulting genome topology. The work yielded the first demonstration of the existence of stem and circle loops in a genome and confirms a model which had been posited based on extensive prior genetic work, providing insights into how 3D genome topologies affect enhancer-promoter communication. The evidence is solid, although the degree of generalization remains uncertain.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  9. Chromosome Structure I: Loop extrusion or boundary:boundary pairing?

    This article has 8 authors:
    1. Xinyang Bing
    2. Wenfan Ke
    3. Miki Fujioka
    4. Amina Kurbidaeva
    5. Sarah Levitt
    6. Mike Levine
    7. Paul Schedl
    8. James B. Jaynes
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable work presents elegant experimental data from the Drosophila embryo supporting the notion that interactions among specific loci, called boundary elements, contribute to topologically associated domain (TAD) formation and gene regulation. The evidence supporting boundary:boundary pairing as a determinant of 3D structures is compelling; however, an inability to deplete loop extruders formally leaves open a possible contribution of loop extrusion. This study will be of interest to the nuclear structure community, particularly those using Drosophila as a model.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  10. Poseidon – A framework for archaeogenetic human genotype data management

    This article has 6 authors:
    1. Clemens Schmid
    2. Ayshin Ghalichi
    3. Thiseas C. Lamnidis
    4. Dhananjaya B. A. Mudiyanselage
    5. Wolfgang Haak
    6. Stephan Schiffels
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper describes an important software framework for the curation, retrieval, and analysis of ancient human genomic data and their associated metadata, overcoming long-standing coordination and harmonization issues in ancient human genomics. The resource is built on compelling and sometimes exceptional principles of software engineering and reproducibility, and the authors make an excellent case that their resource will be of practical use to many researchers studying human history using DNA. The main issues include natural uncertainties regarding future funding and maintenance of this resource, as well as deviation from established standards in other areas of genomics.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity