Developmental Biology

Rejuvenation-Responsive and Senolytic-Sensitive Muscle Stem Cells Unveiled by CD200 and CD63 in Geriatric Muscle

1. Ye Lynne Kim
2. Young-Woo Jo
3. Takwon Yoo
4. Kyusang Yoo
5. Ji-Hoon Kim
6. Myungsun Park
7. In-Wook Song
8. Hyun Kim
9. Yea-Eun Kim
10. Sang-Hyeon Hann
11. Jong-Eun Park
12. Daehyun Baek
13. Young-Yun Kong

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into aged muscle stem cell biology by revealing phenotypic and functional heterogeneity within the geriatric MuSC pool and proposing a VCam-low/negative subpopulation that may account for the reported decline in MuSC numbers with age. These findings have implications for understanding aging-related changes in stem cell maintenance and for improving strategies to isolate or rejuvenate aged MuSCs. However, the evidence supporting the main claims is incomplete, key analyses such as absolute MuSC quantification, fate assessment of VCam-low/negative cells, inclusion of standard aged cohorts, and validation of proposed surface markers are still needed to confirm that overall MuSC abundance is maintained and that a distinct subpopulation has been identified.

Reviewed by eLife

The protein-tyrosine phosphatase Shp2 is essential for lymphatic endothelial cell differentiation in zebrafish

1. Daniëlle T.J. Woutersen
2. Andreas van Impel
3. Stefan Schulte-Merker
4. Jeroen den Hertog

Reviewed by Review Commons

Transcriptional repression of central spindle factors controls endomitosis in the C. elegans intestine

1. Ramon Barrull-Mascaró
2. Sonia Veltkamp
3. Samia Boutaibi
4. Lotte M. van Rijnberk
5. Rebecca Lippmann
6. Matilde Galli

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Chromatin priming and Hunchback recruitment integrate spatial and temporal cues in Drosophila neuroblasts

1. Ayanthi Bhattacharya
2. Hemalatha Rao
3. Sonia Q Sen

• Curated by eLife

  eLife assessment

  The study provides an important advance towards understanding how spatial and temporal transcriptional programs are integrated to regulate lineage-specific chromatin and enhancer activation. The functional evidence is currently incomplete, but the current data provide a solid correlative and conceptual foundation. Functional experiments directly linking Gsb occupancy to chromatin state and regulation of some lineage-specific targets would further strengthen the causal interpretation of the model. Clarifying the scope of conclusions and explicitly acknowledging the technical limitations of current chromatin assays would provide a more balanced interpretation of the manuscript.

Reviewed by eLife

Wnt/β-catenin is required for proprioception by coordinating the multi-compartment development of muscle spindles

1. Qingyuan Guo
2. Ava Gatterer
3. Avital Rosner
4. Sharon Krief
5. Fabian S. Passini
6. Ron Rotkopf
7. Noa Wigoda
8. Michael Tsoory
9. Bavat Bornstein
10. Elazar Zelzer

Reviewed by Review Commons

A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish

1. Rebecca Wafer
2. Panna Tandon
3. James Minchin

• Curated by eLife

  eLife Assessment

  In this manuscript, Wafer and Tandon et al. present a thoughtful and well-designed genetic screen for regulators of adipose remodeling using zebrafish as a model system. This work is valuable because it uncovers several genes associated with adipose tissue hyperplastic hypertrophic morphology and diet-induced remodelingthe hat have considerable potential health impact. The rigorous phenotypic analyses and compelling evidence make this work a key resource for the field.

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Cdhr1a and pcdh15b may link photoreceptor outer segments with calyceal processes revealing a potential mechanism for cone-rod dystrophy

1. Meet K Patel
2. Warlen Pereira Piedade
3. Jakub K Famulski

• Curated by eLife

  eLife Assessment

  This valuable study investigates the interaction of two integral membrane proteins (Cdhr1a and Pcdh15b) and their roles in cone-rod dystrophy. Convincing evidence using loss-of-function mutants demonstrates clearly that both proteins are required for cone maintenance and survival. Although some evidence (Western blots and cell aggregation assays) demonstrates Cdhr1a and Pcdh15b can physically interact, there is insufficient evidence to support the subcellular localization and the proposed heterodimeric interaction of the two proteins from distinct subcellular compartments in cone photoreceptors.

Reviewed by eLife

Foxe1 orchestrates thyroid and lung cell lineage divergence in mouse stem cell-derived organoids

1. Barbara F. Fonseca
2. Cindy Barbée
3. Mirian Romitti
4. Sema Elif Eski
5. Pierre Gillotay
6. Daniel Monteyne
7. David Perez Morga
8. Samuel Refetoff
9. Sumeet Pal Singh
10. Sabine Costagliola

Reviewed by Review Commons

ANTIPODE Provides a Global View of Cell Type Homology and Transcriptomic Divergence in the Developing Mammalian Brain

1. Matthew T Schmitz
2. Jingwen W Ding
3. Sara Nolbrant
4. Reed McMullen
5. Chang N Kim
6. Bryan J Pavlovic
7. Tomasz J Nowakowski
8. Trygve E Bakken
9. Chun Jimmie Ye
10. Alex A Pollen

• Curated by eLife

  eLife Assessment

  This valuable study is an approach to integrating and comparing single-cell genomics data across species. The evidence supporting the conclusions of this work is solid, and ANTIPODE presents an updated methodological approach to determining how gene expression at the cell-type level has evolved. Thus, ANTIPODE should provide broad utility to studies of comparative neurogenomics and be of use to neuroscientists and evolutionary biologists.

Reviewed by eLife

A minimal tooth enhancer regulates dlx2b expression during zebrafish tooth formation: insights into cis-regulatory logic in organogenesis

1. William R Jackman
2. Yujin Moon
3. Carol K Cox
4. Drew R Anderson
5. Audrey A DeFusco
6. Vy M Nguyen
7. Sarah Y Liu
8. Elisabeth H Carter
9. Hana E Littleford
10. Elizabeth K Richards
11. Andrea L Jowdry
12. Yann Gibert

• Curated by eLife

  eLife Assessment

  This study provides valuable findings in the study of enhancer biology by identifying and dissecting a minimal enhancer regulating dlx2b expression during zebrafish tooth development, supported by promoter dissection, reporter assays, and genome-editing approaches. The work offers a resource and extends previous findings but has limited broader impact, with several conclusions about general cis-regulatory principles and functional consequences remaining only partially supported. Accordingly, the strength of evidence is at present incomplete, as additional functional validation would be needed to fully substantiate some of the claims.

Reviewed by eLife