Developmental Biology

Dissecting infant leukemia developmental origins with a hemogenic gastruloid model

1. Denise Ragusa
2. Chun-Wai Suen
3. Gabriel Torregrosa-Cortés
4. Fabio Pastorino
5. Ayona Johns
6. Ylenia Cicirò
7. Liza Dijkhuis
8. Susanne van den Brink
9. Michele Cilli
10. Connor Byrne
11. Giulia-Andreea Ionescu
12. Joana Cerveira
13. Kamil R Kranc
14. Victor Hernandez-Hernandez
15. Mirco Ponzoni
16. Anna Bigas
17. Jordi Garcia-Ojalvo
18. Alfonso Martinez Arias
19. Cristina Pina

• Curated by eLife

  eLife Assessment

  This valuable study presents a mouse gastruloid model that can be used to generate hematopoietic progenitors as well as leukemic cells. However, in its current form, the manuscript is inadequate because the primary claims are not supported. Overall, the hematopoietic progenitor cells generated in this system need to be better defined.

Reviewed by eLife

SMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state

1. Ruben Sebastian-Perez
2. Shoma Nakagawa
3. Xiaochuan Tu
4. Sergi Aranda
5. Martina Pesaresi
6. Pablo Aurelio Gomez-Garcia
7. Marc Alcoverro-Bertran
8. Jose Luis Gomez-Vazquez
9. Davide Carnevali
10. Eva Borràs
11. Eduard Sabidó
12. Laura Martin
13. Malka Nissim-Rafinia
14. Eran Meshorer
15. Maria Victoria Neguembor
16. Luciano Di Croce
17. Maria Pia Cosma

• Curated by eLife

  eLife Assessment

  This important study examines heterochromatin domain dynamics using a model system that allows reversible transition from an embryonic stem cell to a 2-cell-like state. The authors present a solid resource to the research community that will further the understanding of changes in the chromatin-bound proteome during the 2C-to-ESC transition. However, conclusions related to the functional roles of the interaction between the SWI/SNF complex component SMARCAD1 and the DNA Topoisomerase II Binding protein (TOPBP1) remain incomplete.

Reviewed by eLife

Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition

1. Yuhkoh Satouh
2. Takaki Tatebe
3. Isei Tanida
4. Junji Yamaguchi
5. Yasuo Uchiyama
6. Ken Sato

• Curated by eLife

  eLife Assessment

  This paper reports important findings on giant organelle complexes containing endosomes and lysosomes (termed endosomal-lysosomal organelles form assembly structures [ELYSAs]) present in mouse oocytes and 1- to 2-cell embryos. The data showing the localization and dynamics of ELYSAs during oocyte/embryo maturation are convincing. This work will be of interest to general cell biologists and developmental biologists.

Reviewed by eLife

Combined forces of hydrostatic pressure and actin polymerization drive endothelial tip cell migration and sprouting angiogenesis

1. Igor Kondrychyn
2. Liqun He
3. Haymar Wint
4. Christer Betsholtz
5. Li-Kun Phng

• Curated by eLife

  eLife Assessment

  This study convincingly shows that aquaporin-mediated cell migration plays a key role in blood vessel formation during zebrafish development. In particular, the paper implicates hydrostatic pressure and water flow as mechanisms controlling endothelial cell migration during angiogenic sprouting. This fundamental study is highly novel and significantly advances our understanding of cell migration during morphogenesis. As such, this work will be of great interest to developmental and cell biologists working on organogenesis, angiogenesis, and cell migration.

Reviewed by eLife

Impaired yolk sac NAD metabolism disrupts murine embryogenesis with relevance to human birth defects

1. Kayleigh Bozon
2. Hartmut Cuny
3. Delicia Z Sheng
4. Ella MMA Martin
5. Alena Sipka
6. Paul Young
7. David T Humphreys
8. Sally L Dunwoodie

• Curated by eLife

  eLife Assessment

  NAD deficiency perturbs embryonic development resulting in multiple congenital malformations, collectively termed Congenital NAD Deficiency Disorder (CNDD). The authors report fundamental findings demonstrating that extra-embryonic visceral yolk sac endoderm is critical for NAD de novo synthesis during early organogenesis and perturbations of this pathway may underlie CNDD. The authors combine gene expression with metabolic assays to provide solid evidence of an essential role of the extra-embryonic visceral yolk sac in both mouse and human embryos.

Reviewed by eLife

Single-nucleus multiomics reveals the gene-regulatory networks underlying sex determination of murine primordial germ cells

1. Adriana K Alexander
2. Karina F Rodriguez
3. Yu-Ying Chen
4. Ciro M Amato
5. Martin A Estermann
6. Barbara Nicol
7. Xin Xu
8. Humphrey Hung-Chang Yao

• Curated by eLife

  eLife Assessment

  This important study reports single-nucleus multiomics-based profiling of transcriptome and chromatin accessibility of mouse XX and XY primordial germ cells (PGCs). The main conclusions of this study, which will be of interest to developmental and reproductive biologists, as well as andrologists, are supported by convincing data.

Reviewed by eLife

Live-imaging reveals Coordinated Cell Migration and Cardiac Fate Determination during Mammalian Gastrulation

1. Shayma Abukar
2. Peter A. Embacher
3. Alessandro Ciccarelli
4. Sunita Varsani-Brown
5. Isabel G.W. North
6. Jamie A. Dean
7. James Briscoe
8. Kenzo Ivanovitch

Reviewed by Review Commons

UNC-6/Netrin promotes both adhesion and directed growth within a single axon

1. Ev L Nichols
2. Joo Lee
3. Kang Shen

• Curated by eLife

  eLife Assessment

  These studies make a fundamental contribution to our understanding of axon-guidance mechanisms, focusing on the role of UNC-6/Netrin in the long-range growth and targeting of axons. Using state-of-the-art genetics and in vivo imaging, the authors provide compelling support for the finding that UNC-6/Netrin can act via both chemotaxis and haptotaxis. This work will be of interest to a wide variety of cell and developmental biologists and neuroscientists.

Reviewed by eLife

GLE1 dysfunction compromises cellular homeostasis, spatial organization, and peripheral axon branching

1. T Zárybnický
2. S Lindfors
3. S Metso
4. Z Szabo
5. R Valtonen
6. M Tulppo
7. J Magga
8. S Saarimäki
9. S Bläuer
10. I Miinalainen
11. R Kerkelä
12. J Väänänen
13. R Kivelä
14. FP Zhang
15. P Sipilä
16. R Hinttala
17. S Kuure

Reviewed by Review Commons

Meioc-Piwil1 complexes regulate rRNA transcription for differentiation of spermatogonial stem cells

1. Toshihiro Kawasaki
2. Toshiya Nishimura
3. Naoki Tani
4. Carina Ramos
5. Emil Karaulanov
6. Minori Shinya
7. Kenji Saito
8. Emily Taylor
9. Rene F. Ketting
10. Kei-ichiro Ishiguro
11. Minoru Tanaka
12. Kellee R. Siegfried
13. Noriyoshi Sakai

• Curated by eLife

  eLife Assessment

  This valuable paper describes the regulatory pathway of rRNA synthesis by Meioc-Piwil1 in germ cell differentiation in zebrafish. Using the molecular genetic and cytological approaches, the authors provide convincing evidence that Meioc antagonizes Piwil1, which downregulates the 45S pre-rRNA synthesis by heterochromatin formation for spermatocyte differentiation. The key results are solid and of use to researchers in the field of germ cell/meiosis as well as RNA biosynthesis and chromatin.

Reviewed by eLife