Developmental Biology

Genetic compensation in podocalyxin-like mutants during zebrafish liver development

1. Alexis N Ross
2. Natalie M Miscik
3. Sharanya Maanasi Kalasekar
4. James D Harris
5. Mimi Tran
6. Aavrati Saxena
7. Steven Andrew Baker
8. Kimberley Jane Evason

• Curated by eLife

  eLife Assessment

  The manuscript by Ross, Miscik, and others describes an intriguing series of observations made when investigating the requirement for podxl during hepatic development in zebrafish. Understanding how genetic compensation pathways are involved in gene function is an important question. However, there is incomplete evidence provided in the manuscript at this point to conclude that discrepancies between observed phenotypes are due to genetic compensation.

Reviewed by eLife

Glial betaPix is essential for blood vessel integrity in the zebrafish brain

1. ShihChing Chiu
2. Qinchao Zhou
3. Chenglu Xiao
4. Linlu Bai
5. Xiaojun Zhu
6. Wanqiu Ding
7. Jing-Wei Xiong

• Curated by eLife

  eLife assessment

  This valuable manuscript presents findings supported by solid data to identify a surprising glia-exclusive function for betapix in vascular integrity and angiogenesis. The manuscript also describes the optimisation of a modified CRISPR-based Zwitch approach to generate conditional knockouts in zebrafish.

Reviewed by eLife

Organization of the apical extracellular matrix during tubular organ formation

1. J. Luke Woodward
2. Jeffrey Matthew
3. Rutuparna Joshi
4. Vishakha Vishwakarma
5. Ying Xiao
6. SeYeon Chung

Reviewed by Review Commons

The essential role of connective-tissue cells during axolotl limb regeneration

1. Damián García-García
2. Dunja Knapp
3. Minjoo Kim
4. Katelyn Jamwal
5. Heath Fuqua
6. Ryan P. Seaman
7. Riley E. Grindle
8. Sergej Nowoshilow
9. Maria Novatchkova
10. Fred W. Kolling
11. Joel H. Graber
12. Prayag Murawala

Reviewed by preLights

Alteration of long and short-term hematopoietic stem cell ratio causes myeloid-biased hematopoiesis

1. Katsuyuki Nishi
2. Taro Sakamaki
3. Akiomi Nagasaka
4. Kevin S Kao
5. Kay Sadaoka
6. Masahide Asano
7. Nobuyuki Yamamoto
8. Akifumi Takaori-Kondo
9. Masanori Miyanishi

• Curated by eLife

  eLife Assessment

  This manuscript provides valuable insights into the heterogeneity of hematopoietic stem cells and age-associated myeloid-biased hematopoiesis. While several aspects of the study are intriguing and merit further investigation, the current results remain incomplete and additional data are necessary to substantiate the conclusions. Some of the methods and data analyses partially support the claims.

Reviewed by eLife, preLights

FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

1. Rosanna CG Smith
2. Maryam Clark
3. Mireya Vazquez-Prada
4. Marc Astick
5. Kristina C Tubby
6. Stephen R Price

• Curated by eLife

  eLife Assessment

  The authors conducted a valuable study that investigates a molecular pathway mediating the transformation of a cell aggregate into a sheet known as the nucleus laminaris, a crucial site for auditory processing. While the study offers a comprehensive view of the sequence of developmental events and suggests possible roles for FGF signaling, the transcription factor Mafb, and the cell surface adhesive molecule Cadherin-23 in this process, the current data were considered incomplete.

Reviewed by eLife

HIF1A contributes to the survival of aneuploid and mosaic pre-implantation embryos

1. Estefania Sanchez-Vasquez
2. Marianne E Bronner
3. Magdalena Zernicka-Goetz

• Curated by eLife

  eLife Assessment

  Sanchez-Vasquez et al establish an innovative approach to induce aneuploidy in preimplantation embryos. This important study extends the author's previous publications evaluating the consequences of aneuploidy in the mammalian embryo. In this work, the authors investigate the developmental potential of aneuploid embryos and characterize changes in gene expression profiles under normoxic and hypoxic culture conditions. Using a solid methodology they identify sensitivity to Hif1alpha loss in aneuploid embryos, and in further convincing experiments they assess how levels of DNA damage and DNA repair are altered under hypoxic and normoxic conditions.

Reviewed by eLife, preLights

Cardiac fibroblasts regulate myocardium and coronary vasculature development in the murine heart via the collagen signaling pathway

1. Yiting Deng
2. Yuanhang He
3. Juan Xu
4. Haoting He
5. Manling Zhang
6. Guang Li

• Curated by eLife

  eLife Assessment

  This study provides a comprehensive analysis of gene expression and bioinformatics data, offering important insights into the roles of fibroblasts in cardiac development. The large and well-analyzed single-cell RNA sequencing (scRNA-seq) dataset is compelling and a significant contribution to the field, and will be of broad interest to the scientific community.

Reviewed by eLife

The gut contractile organoid: a novel model for studying the gut motility regulated by coordinating signals between interstitial cells of Cajal and smooth muscles

1. Rei Yagasaki
2. Ryo Nakamura
3. Yuuki Shikaya
4. Ryosuke Tadokoro
5. Ruolin Hao
6. Zhe Wang
7. Mototsugu Eiraku
8. Masafumi Inaba
9. Yoshiko Takahashi

• Curated by eLife

  eLife Assessment

  This valuable study reports the development of a novel organoid system for studying the emergence of autorhythmic gut peristaltic contractions through the interaction between interstitial cells of Cajal and smooth muscle cells. The authors further utilized the system to provide convincing evidence for a previously unappreciated potential role for smooth muscle cells in regulating the firing rate of interstitial cells of Cajal. The work will be of interest to those studying development and physiology of the gut.

Reviewed by eLife

Decoding protein phosphorylation during oocyte meiotic divisions using phosphoproteomics

1. Leonid Peshkin
2. Enrico Maria Daldello
3. Elizabeth Van Itallie
4. Matthew Sonnett
5. Johannes Kreuzer
6. Wilhelm Haas
7. Marc W Kirschner
8. Catherine Jessus

• Curated by eLife

  eLife Assessment

  This important paper describes a comprehensive quantitative phospho-proteomic analysis of the meiotic progression of Xenopus oocytes. Using time-resolved proteomic analyses, the authors provide insights into changes in protein levels and phosphorylation states to an unprecedented depth, quality, and quantitative detail. The key findings are compelling and offer a helpful resource for the scientific community.

Reviewed by eLife