Developmental Biology

NR2F2 is required in the embryonic testis for Fetal Leydig Cell development

1. Aitana Perea-Gomez
2. Natividad Bellido-Carreras
3. Magali Dhellemmes
4. Furong Tang
5. Coralie Le Gallo
6. Marie-Christine Chaboissier

Reviewed by eLife

Meteorins regulate the formation of the left-right organizer and the establishment of vertebrate body asymmetry

1. Fanny Eggeler
2. Jonathan Boulanger-Weill
3. Flavia De Santis
4. Laura Belleri
5. Karine Duroure
6. Thomas O Auer
7. Shahad Albadri
8. Filippo Del Bene

• Curated by eLife

  eLife Assessment

  This study presents important insights into the regulation of left-right organ formation. By combining genetic perturbation of all three Meteorin genes in zebrafish and timelapse imaging, the authors identify an essential role for this protein family in the establishment of left-right patterning. They provide convincing evidence that Meteorins are required for the morphogenesis of dorsal forerunner cells, the precursors of the left-right organizer (also named Kupffer's vesicle) in zebrafish. In line with this, Meteorins were shown to genetically interact with integrins ItgaV and Itgb1b to regulate dorsal forerunner cell clustering.

Reviewed by eLife

Argonaute proteins regulate the timing of the spermatogenic transcriptional program

1. Maria de las Mercedes Carro
2. Alexis Dziubek
3. Amanda Touey
4. Elizabeth A. Popkowski
5. Mark Abdelmassih
6. Leah Simon
7. Stephanie Tanis
8. Faraz Ahmed
9. Jen K. Grenier
10. Andrew Grimson
11. Paula E. Cohen

Reviewed by Review Commons

Adhesion to a common ECM mediates interdependence in tissue morphogenesis in Drosophila

1. LE Sánchez-Cisneros
2. M Barrera-Velázquez
3. Dimitri Kromm
4. P Bun
5. H Merchant-Larios
6. LD Ríos-Barrera

Reviewed by Review Commons

Mechanical fracturing of the extracellular matrix patterns the vertebrate heart

1. Christopher Chan Jin Jie
2. Daniel Santos-Oliván
3. Marie-Christine Ramel
4. Juliana Sánchez-Posada
5. Toby G R Andrews
6. Priscilla Paizakis
7. Emily S Noël
8. Alejandro Torres-Sánchez
9. Rashmi Priya

Reviewed by preLights

Identification and characterization of early human photoreceptor states and cell-state-specific retinoblastoma-related features

1. Dominic WH Shayler
2. Kevin Stachelek
3. Linda Cambier
4. Sunhye Lee
5. Jinlun Bai
6. Bhavana Bhat
7. Mark W Reid
8. Daniel J Weisenberger
9. Jennifer G Aparicio
10. Yeha Kim
11. Mitali Singh
12. Maxwell Bay
13. Matthew E Thornton
14. Eamon K Doyle
15. Zachary Fouladian
16. Stephan G Erberich
17. Brendan H Grubbs
18. Michael A Bonaguidi
19. Cheryl Mae Craft
20. Hardeep P Singh
21. David Cobrinik

• Curated by eLife

  eLife Assessment

  In this important paper, the authors use single-cell RNA sequencing to understand post-mitotic cone and rod developmental states and identify cone-specific features that contribute to retinoblastoma genesis. The authors report findings that have practical implications for retinal development, gene expression, and cell fate specification. The evidence is compelling as the experimental design and analysis are exceptionally rigorous.

Reviewed by eLife

Redistribution of fragmented mitochondria ensure symmetric organelle partitioning and faithful chromosome segregation in mitotic mouse zygotes

1. Haruna Gekko
2. Ruri Nomura
3. Daiki Kuzuhara
4. Masato Kaneyasu
5. Genpei Koseki
6. Deepak Adhikari
7. Yasuyuki Mio
8. John Carroll
9. Tomohiro Kono
10. Hiroaki Funahashi
11. Takuya Wakai

• Curated by eLife

  eLife Assessment

  This important study investigates the role of Drp1 in early embryo development. The authors have addressed most of the original comments and the work now presents convincing evidence on how this protein influences mitochondrial localization and partitioning during the first embryonic divisions. The research employs the Trim-Away technique to eliminate Drp1 in zygotes, revealing critical insights into mitochondrial clustering, spindle formation, and embryonic development.

Reviewed by eLife

Pu.1/Spi1 dosage controls the turnover and maintenance of microglia in zebrafish and mammals

1. Yi Wu
2. Weilin Guo
3. Haoyue Kuang
4. Xiaohai Wu
5. Thi Huong Trinh
6. Yuexin Wang
7. Shizheng Zhao
8. Zilong Wen
9. Tao Yu

• Curated by eLife

  eLife Assessment

  This study presents valuable findings on the regulation of survival and maintenance of brain-resident immune cells called microglia. Using compelling and sophisticated genetic tools, the authors demonstrate a gene dosage-dependent mechanism using which microglia are eliminated. This research on cell competition and survival will be of broad interest to the cell biology community.

Reviewed by eLife

Microtubule networks in zebrafish hair cells facilitate presynapse transport and fusion during development

1. Saman Hussain
2. Katherine Pinter
3. Mara Uhl
4. Hiu-Tung Wong
5. Katie S Kindt

• Curated by eLife

  eLife Assessment

  This fundamental study provides new insights into the maturation of ribbon synapses in zebrafish neuromast hair cells. Live-cell imaging and pharmacological and genetic manipulations together provide compelling evidence that the formation of this synaptic organelle is a dynamic process involving the fusion of presynaptic elements and microtubule transport, though the evidence that ribbon precursors move in a directed motion toward the active zone is less persuasive. These findings will be of interest to neuroscientists studying synapse formation and function and should inspire further research into the molecular basis for synaptic ribbon maturation.

Reviewed by eLife

The oocyte zinc transporter Slc39a10/Zip10 is a regulator of zinc sparks during fertilization in mice

1. Atsuko Kageyama
2. Narumi Ogonuki
3. Takuya Wakai
4. Takafumi Namiki
5. Yui Kawata
6. Manabu Ozawa
7. Yasuhiro Yamada
8. Toshiyuki Fukada
9. Atsuo Ogura
10. Rafael A Fissore
11. Naomi Kashiwazaki
12. Junya Ito

• Curated by eLife

  eLife Assessment

  This study presents significant and novel insights into the roles of zinc in mammalian meiosis/fertilization events. These findings are useful to our understanding of these processes. The evidence presented is solid, with experiments being well-designed, carefully described, and interpreted with appropriate rigor.

Reviewed by eLife