Developmental Biology

Matrix Rigidity Governs Switch-Like Oscillatory State Transitions of the Segmentation Clock in Isolated Presomitic Mesoderm Cells

1. Chun-Yen Sung
2. Yuqian Yang
3. Usha Kadiyala
4. Owen Blanchard
5. Liam Yourston
6. Derek Walker
7. Linyuan Li
8. Shiyu Sun
9. Zhaoyi Xu
10. Mariela Lopez Gonzalez
11. Jianping Fu
12. Qiong Yang

Reviewed by preLights

Single-cell spatial mapping reveals reproducible cell type organization and spatially-dependent gene expression in gastruloids

1. Catherine G Triandafillou
2. Pranav Sompalle
3. Yael Heyman
4. Arjun Raj

• Curated by eLife

  eLife Assessment

  This work presents important findings on quantifying gene coexpression from spatial omics. These quantification methods have been applied to gastruloid to describe how genes are spatialised. The description of the quantifying tools is characterized by exceptional evidence after a thorough revision.

Reviewed by eLife

Dissecting PADI6 function defines oocyte cytoplasmic lattices as regulatory hubs for fundamental cellular processes

1. Jack P. C. Williams
2. Tania Auchynnikava
3. Afshan McCarthy
4. M. Teresa Bertran
5. Obah A. Ojarikre
6. Anne E. Weston
7. Daniel Leonce
8. Jessica Olsen
9. Kathy K. Niakan
10. Mark Skehel
11. James M. A. Turner
12. Louise J. Walport

Reviewed by Review Commons

Gcn5 – mTORC1 – TFEB signalling axis mediated control of autophagy regulates Drosophila blood cell homeostasis

1. Arjun Aji Reeja
2. Mincy Kunjumon
3. Suraj Math
4. Laxmi Kata
5. Rohan Jayant Khadilkar

• Curated by eLife

  eLife Assessment

  This valuable study identifies Gcn5 as a regulator of blood cell development in the Drosophila lymph gland, with links to autophagy and nutrient-sensing mTORC1 signalling. The evidence is solid that altering Gcn5, autophagy genes and mTORC1 activity perturbs blood cell homeostasis, and the revised manuscript adds helpful genetic and quantitative analyses. However, the evidence for a clean linear Gcn5-mTORC1-TFEB/autophagy pathway is insufficient, because several cell-type-specific phenotypes remain difficult to reconcile and the pathway logic relies on different genetic tools, cell populations and pharmacological perturbations.

Reviewed by eLife

Trpv4 links environmental temperature to testicular differentiation in hermaphroditic ricefield eel

1. Yang Zhi
2. Luo Tingting
3. Zhang Yimin
4. Sun Yuhua

• Curated by eLife

  eLife Assessment

  This study presents useful findings on the molecular mechanisms driving female-to-male sex reversal in the ricefield eel (Monopterus albus) during aging, which would be of interest to biologists studying sex determination. The manuscript describes an interesting mechanism potentially underlying sex differentiation in M. albus. However, the current data are incomplete and would benefit from more rigorous experimental approaches for Western blotting.

Reviewed by eLife

Paternal over- and under-nutrition programme fetal and placental development in a sex-specific manner in mice

1. Hannah L Morgan
2. Nader Eid
3. Nadine Holmes
4. Matthew Carlile
5. Sonal Henson
6. Fei Sang
7. Victoria Wright
8. Marcos Castellanos-Uribe
9. Iqbal Khan
10. Nazia Nazar
11. Sean T May
12. Rod T Mitchell
13. Federica Lopes
14. Robert S Robinson
15. A Augusto Coppi
16. Vipul Batra
17. Adam J Watkins

• Curated by eLife

  eLife Assessment

  This important study demonstrates that paternal diet influences not only testicular morphology but also placental and fetal development, supporting a role for paternal contributions to offspring health. The study also considers potential links between the microbiome and male reproductive health. By combining transcriptomic and histological analyses across multiple tissues, the evidence supporting the central conclusions of the study is convincing.

Reviewed by eLife