Developmental Biology

Cardiac Fibroblasts regulate myocardium and coronary vasculature development via the collagen signaling pathway

1. Yiting Deng
2. Yuanhang He
3. Juan Xu
4. Haoting He
5. Manling Zhang
6. Guang Li

• Curated by eLife

  eLife Assessment

  The study presents extensive gene expression profiling and bioinformatic analyses, offering insights into the roles of fibroblasts in cardiac development. The large volume of scRNA-seq data is both compelling and important to the scientific community. All three reviewers agree that the revised manuscript represents a significant improvement and addresses most, if not all, of their previous concerns. The reviewers also acknowledge that detailed mechanistic studies on how fibroblast-derived collagen regulates myocardial and coronary vasculature development are beyond the scope of the current study.

Reviewed by eLife

The integration mechanism of the four positional cues—anterior, posterior, dorsal, and ventral—in axolotl limb regeneration

1. Sakiya Yamamoto
2. Saya Furukawa
3. Ayaka Ohashi
4. Mayuko Hamada
5. Akira Satoh

• Curated by eLife

  eLife Assessment

  This fundamental work by Yamamoto and colleagues advances our understanding of how positional information is coordinated between axes during limb outgrowth and patterning. They provide solid evidence that the dorsal-ventral axis feeds into anterior-posterior signaling, and identify the responsible molecules by combining transplantations with molecular manipulations. This work will be of broad interest to regeneration, tissue engineering, and evolutionary biologists.

Reviewed by eLife

Linage priming and cell type proportioning depends on the interplay between stochastic and deterministic factors

1. William M Salvidge
2. Chris Brimson
3. Nicole Gruenheit
4. Li-Yao Huang
5. Catherine J Pears
6. Jason B Wolf
7. Christopher RL Thompson

• Curated by eLife

  eLife Assessment

  This valuable study investigates how stochastic and deterministic factors are integrated during cellular decision-making, particularly in situations where cells differentiate into distinct fates despite being exposed to the same environmental conditions. The authors present convincing evidence that gene expression variability contributes to the robustness of cell fate decisions in D. discoideum, which sheds light into the role of stochasticity during cell differentiation.

Reviewed by eLife

SVEP1 enables efficient binding of Angiopoietin-2 to the TIE1 receptor, allowing receptor phosphorylation and downstream signaling

1. Katharina Uphoff
2. Melina Hußmann
3. Dörte Schulte-Ostermann
4. Yvonne Huisman
5. Matthias Mörgelin
6. Fabian Metzen
7. J Fernando Bazan
8. Manuel Koch
9. Stefan Schulte-Merker

• Curated by eLife

  eLife Assessment

  This manuscript focuses on developing a structural model of how the multidomain ECM protein SVEP1 enables Angiopoietin (ANG) binding to the orphan receptor TIE1, resulting in downstream receptor phosphorylation and signaling. This is a potentially important study, however, it currently lacks key controls and is therefore incomplete. The data will be of interest to scientists working in vascular biology and RTK signaling.

Reviewed by eLife

An increase in reactive oxygen species underlies neonatal cerebellum repair

1. Anna Pakula
2. Salsabiel El Nagar
3. N Sumru Bayin
4. Jens Bager Christensen
5. Daniel N Stephen
6. Adam James Reid
7. Richard Koche
8. Alexandra L Joyner

• Curated by eLife

  eLife Assessment

  This important work substantially advances our understanding of reactive oxygen species (ROS) as a regenerative signal during postnatal cerebellum repair by activating adaptive progenitor reprogramming. The evidence supporting the conclusions is compelling, with rigorous genomic assays and in vivo analyses. This work will be of broad interest to biologists working on stem cells, neurodevelopment and regenerative medicine.

Reviewed by eLife

Integrated transcriptomic analysis of human induced pluripotent stem cell-derived osteogenic differentiation reveals a regulatory role of KLF16

1. Ying Ru
2. Meng Ma
3. Xianxiao Zhou
4. Divya Kriti
5. Ninette Cohen
6. Sunita D’Souza
7. Christoph Schaniel
8. Susan M Motch Perrine
9. Sharon Kuo
10. Oksana Pichurin
11. Dalila Pinto
12. Genevieve Housman
13. Greg Holmes
14. Eric Schadt
15. Harm van Bakel
16. Bin Zhang
17. Ethylin Wang Jabs
18. Meng Wu

• Curated by eLife

  eLife Assessment

  The authors investigated KLF Transcription Factor 16 (KLF16) as an inhibitor of osteogenic differentiation, which plays a critical role in bone development, metabolism and repair. The results of the study are valuable as they could help to facilitate future research on the regulation of osteogenesis in vitro and in vivo. However, the evidence overall is incomplete, as validation by knockout mouse models would help to strengthen the conclusions.

Reviewed by eLife

Progressive mural cell deficiencies across the lifespan in a foxf2 model of Cerebral Small Vessel Disease

1. Merry Faye E Graff
2. Emma EM Heeg
3. Sarah J Childs

• Curated by eLife

  eLife Assessment

  This study presents valuable findings that advance our understanding of mural cell dynamics and vascular pathology in a zebrafish model of cerebral small vessel disease. The authors provide compelling evidence that partial loss of foxf2 function leads to progressive, cell-intrinsic defects in pericytes and associated endothelial abnormalities across the lifespan, leveraging powerful in vivo imaging and genetic tools. The strength of evidence could be further improved by additional mechanistic insight and quantitative or lineage-tracing analyses to clarify how pericyte number and identity are affected in the mutant model.

Reviewed by eLife

BCAS2 promotes primitive hematopoiesis by sequestering β-catenin within the nucleus

1. Guozhu Ning
2. Yu Lin
3. Haixia Ma
4. Jiaqi Zhang
5. Liping Yang
6. Zhengyu Liu
7. Lei Li
8. Xinyu He
9. Qiang Wang

• Curated by eLife

  eLife Assessment

  This important work supports the role of breast carcinoma amplified sequence 2 (Bcas2) in positively regulating primitive wave hematopoiesis through amplification of beta-catenin-dependent (canonical) Wnt signaling. The study is convincing: it uses appropriate and validated methodology in line with the current state-of-the-art, and there is a first-rate analysis of a strong phenotype with highly supportive mechanistic data. The findings shed light on the controversial question of whether, when, and how canonical Wnt signaling may be involved in hematopoietic development. The work will be of interest to hematologists and developmental biologists.

Reviewed by eLife

A myofilament lattice model of Drosophila flight muscle sarcomeres based on multiscale morphometric analysis during development

1. Péter Görög
2. Tibor Novák
3. Tamás F. Polgár
4. Péter Bíró
5. Adél Gutheil
6. Csaba Kozma
7. Tamás Gajdos
8. Krisztina Tóth
9. Alexandra Tóth
10. Miklós Erdélyi
11. József Mihály
12. Szilárd Szikora

Reviewed by Review Commons

The PIDDosome controls cardiomyocyte polyploidization during postnatal heart development

1. M Leone
2. N Kinz
3. F Eichin
4. D Obwegs
5. VC Sladky
6. VZ Braun
7. D Rizzotto
8. L Englmaier
9. C Manzl
10. K Moos
11. Julia Mergner
12. P Giansanti
13. MN Garcia
14. MM Marques
15. ED Jacotot
16. C Savko
17. M Boerries
18. MA Sussman
19. A Villunger

Reviewed by Review Commons