Developmental Biology

Negative cell cycle regulation by Calcineurin is necessary for proper beta cell regeneration in zebrafish

1. Laura Massoz
2. David Bergemann
3. Arnaud Lavergne
4. Célia Reynders
5. Caroline Désiront
6. Chiara Goossens
7. Lydie Flasse
8. Bernard Peers
9. Marianne L. Voz
10. Isabelle Manfroid

• Curated by eLife

  eLife assessment

  This work presents some valuable information regarding the molecular mechanisms controlling the regeneration of pancreatic beta cells following induced cell ablation in zebrafish. Specifically, the data suggest that Calcineurin is a regulator of beta cell regeneration. However, the study lacks the critical lineage tracing results to support the conclusion about the origin of the regenerated beta cells and thus is deemed incomplete.

Reviewed by eLife

Specialized signaling centers direct cell fate and spatial organization in a limb organoid model

1. Evangelia Skoufa
2. Jixing Zhong
3. Oliver Kahre
4. Kelly Hu
5. Georgios Tsissios
6. Louise Carrau
7. Antonio Herrera
8. Albert Dominguez Mantes
9. Alejandro Castilla-Ibeas
10. Hwanseok Jang
11. Martin Weigert
12. Gioele La Manno
13. Matthias Lutolf
14. Marian Ros
15. Can Aztekin

Reviewed by preLights

HvNotch coordinates two independent pattern forming systems during head regeneration in Hydra by supporting a lateral inhibition process restricting the tentacle system

1. Mona Steichele
2. Lara Sauermann
3. Qin Pan
4. Jasmin Moneer
5. Alexandra de la Porte
6. Martin Heß
7. Moritz Mercker
8. Catharina Strube
9. Marcell Jenewein
10. Angelika Böttger

Reviewed by Review Commons

PRMT1-SFPQ regulates intron retention to control matrix gene expression during craniofacial development

1. Julia Raulino Lima
2. Nicha Ungvijanpunya
3. Qing Chen
4. Greg Park
5. Mohammadreza Vatankhah
6. Tal Rosen
7. Yang Chai
8. Amy Merrill-Brugger
9. Weiqun Peng
10. Jian Xu

• Curated by eLife

  eLife assessment

  This study presents an interesting mechanism for the regulation of RNA levels, establishing an important regulatory connection between protein arginine methyltransferase 1 and the splicing factor SFPQ. While these findings have theoretical implications beyond a single field, the evidence is incomplete, with only partial support for the main claims.

Reviewed by eLife

Genetic requirement of dact1/2 to regulate noncanonical Wnt signaling and calpain 8 during embryonic convergent extension and craniofacial morphogenesis

1. Shannon H. Carroll
2. Sogand Schafer
3. Kenta Kawasaki
4. Casey Tsimbal
5. Amélie M. Julé
6. Shawn A. Hallett
7. Edward Li
8. Eric C. Liao

• Curated by eLife

  eLife assessment

  This valuable study confirms the roles of Dact1 and Dact2, two factors involved in Wnt signaling, during zebrafish gastrulation and demonstrates their genetic interactions with other Wnt components to modulate craniofacial morphologies. The limitation of the study is that it does not distinguish primary from secondary effects for each factor, precluding an unambiguous interpretation of their roles in craniofacial morphogenesis. The findings of a new potential target of dact1/2-mediated Wnt signaling are potentially of value; however, experimental evidence supporting their functional significance remains incomplete due to inconsistent results and the inherent limitations of the overexpression study.

Reviewed by eLife

The conserved genetic program of male germ cells uncovers ancient regulators of human spermatogenesis

1. Rion Brattig Correia
2. Joana M Almeida
3. Margot J Wyrwoll
4. Irene Julca
5. Daniel Sobral
6. Chandra Shekhar Misra
7. Sara Di Persio
8. Leonardo G Guilgur
9. Hans-Christian Schuppe
10. Neide Silva
11. Pedro Prudêncio
12. Ana Nóvoa
13. Ana S Leocádio
14. Joana Bom
15. Sandra Laurentino
16. Moisés Mallo
17. Sabine Kliesch
18. Marek Mutwil
19. Luis M Rocha
20. Frank Tüttelmann
21. Jörg D Becker
22. Paulo Navarro-Costa

• Curated by eLife

  eLife assessment

  This fundamental study reports the deep evolutionary conservation of a core genetic program regulating spermatogenesis in flies, mice, and humans. Convincing data were presented and supported the main conclusion. This work will be of interest to evolutionary and reproductive biologists.

Reviewed by eLife

Dynamics of transcriptional programs and chromatin accessibility in mouse spermatogonial cells from early postnatal to adult life

1. Irina Lazar-Contes
2. Rodrigo G Arzate-Mejia
3. Deepak K Tanwar
4. Leonard C Steg
5. Kerem Uzel
6. Olivier Ulrich Feudjio
7. Marion Crespo
8. Pierre-Luc Germain
9. Isabelle M Mansuy

• Curated by eLife

  eLife assessment

  This useful study reports datasets on gene expression and chromatin accessibility profiles of spermatogonia at different postnatal ages in mice. The supporting data are considered incomplete. This study may be of interest to biomedical researchers working on male germline stem cells and male fertility.

Reviewed by eLife

Distinct functions of three Wnt proteins control mirror-symmetric organogenesis in the C. elegans gonad

1. Shuhei So
2. Masayo Asakawa
3. Hitoshi Sawa

Reviewed by Review Commons

Mechanistic target of rapamycin (mTOR) pathway in Sertoli cells regulates age-dependent changes in sperm DNA methylation

1. Saira Amir
2. Olatunbosun Arowolo
3. Ekaterina Mironova
4. Joseph McGaunn
5. Oladele Oluwayiose
6. Oleg Sergeyev
7. J Richard Pilsner
8. Alexander Suvorov

• Curated by eLife

  eLife assessment

  This potentially important study addresses the effects of aging on the sperm epigenome and its consequences for reproductive health. The evidence supporting the main claim remains incomplete. This study will be of interest to researchers working on aging and reproductive health.

Reviewed by eLife

Stimulatory and inhibitory G-protein signaling relays drive cAMP accumulation for timely metamorphosis in the chordate Ciona

1. Akiko Hozumi
2. Nozomu M Totsuka
3. Arata Onodera
4. Yanbin Wang
5. Mayuko Hamada
6. Akira Shiraishi
7. Honoo Satake
8. Takeo Horie
9. Kohji Hotta
10. Yasunori Sasakura

• Curated by eLife

  eLife assessment

  This important work substantially advances our understanding of the molecular mechanisms underlying the timing of the initiation of metamorphosis of the Ciona ascidian tadpole larva. Through the combination of gene knockdown experiments and fluorescent molecular reporters the authors provide compelling evidence about a crosstalk between different G protein mediated signalling pathways and are able to place different signalling molecules within a signalling network. The work will be of interest to molecular, developmental and marine biologists and to scientists working on animal metamorphosis.

Reviewed by eLife