Developmental Biology

Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

1. Çağrı Çevrim
2. Béryl Laplace-Builhé
3. Ko Sugawara
4. Maria Lorenza Rusciano
5. Nicolas Labert
6. Jacques Brocard
7. Alba Almazán
8. Michalis Averof

Reviewed by Review Commons

The retinoic acid receptor regulates development of a key evolutionary novelty - the molluscan shell

1. Keisuke Shimizu
2. Paul A. O’Neill
3. Kazuyoshi Endo
4. Tetsuhiro Kudoh

Reviewed by Arcadia Science

Prenatal exposure to environmental stressors alters gut macrophage development and gastrointestinal function of male offspring

1. Dang M. Nguyen
2. Sarah K. Monroe
3. Danielle N. Rendina
4. Kevin S. Boyd
5. Erika D. Rispoli
6. Olivia M. Wirfel
7. A. Brayan Campos-Salazar
8. Anna R. Araujo
9. Trisha V. Vaidyanathan
10. Virginia L. Keziah
11. Benjamin A. Devlin
12. Caroline J. Smith
13. Staci D. Bilbo

Reviewed by preLights

Gene regulatory mechanisms guiding bifurcation of inhibitory and excitatory neuron lineages in the anterior brainstem

1. Sami Kilpinen
2. Lassi Virtanen
3. Silvana Bodington-Celma
4. Amos Bonsdorff
5. Heidi Heliölä
6. Kaia Achim
7. Juha Partanen

• Curated by eLife

  eLife Assessment

  The authors provide valuable insights into the candidate upstream transcriptional regulatory factors that control the spatiotemporal expression of selector genes and their targets for GABAergic vs glutamatergic neuron fate in the anterior brainstem. The computational analysis of single-cell RNA-seq and single-cell ATAC-seq datasets to predict TF binding combined with cut and tag-seq to find TF binding represents a solid approach to support the findings in the study, although the display and discussion of the datasets could be strengthened. This study will be of interest to neurobiologists who study transcriptional mechanisms of neuronal differentiation.

Reviewed by eLife

Site-specific DNA demethylation during spermatogenesis presets the sites of nucleosome retention in mouse sperm

1. So Maezawa
2. Masashi Yukawa
3. Akihiko Sakashita
4. Artem Barski
5. Satoshi H Namekawa

• Curated by eLife

  eLife Assessment

  This study provides valuable findings on the role of site-specific DNA methylation changes during spermatogenesis and their contribution to paternal epigenetic inheritance. The study proposes that selective loss of DNA methylation at a subset of promoters is required for nucleosome retention and the establishment of epigenetic states that may influence embryonic gene regulation. The present study's conclusion is mostly supported by solid data.

Reviewed by eLife

SRSF3 is a key regulator of epicardial formation

1. Irina-Elena Lupu
2. Susann Bruche
3. Anob M. Chakrabarti
4. Ian R. McCracken
5. Tamara Carsana
6. Andia N. Redpath
7. Nicola Smart

Reviewed by Review Commons

Pleiotropic function of Dlx5/6 in coordinating the development of mammalian vocal and auditory organs

1. Frida Sánchez-Garrido
2. Victoria Bouzerand
3. Markéta Kaiser
4. Chloé Chaumeton
5. Anastasia Fontaine
6. Tomáš Zikmund
7. Jozef Kaiser
8. Églantine Heude

Reviewed by Review Commons

Zebrafish Foxl2l suppresses stemness of germline progenitors and directs feminization

1. Chen-wei Hsu
2. Hao Ho
3. Ching-Hsin Yang
4. Yan-wei Wang
5. Ker-Chau Li
6. Bon-chu Chung

• Curated by eLife

  eLife Assessment

  This manuscript provides important findings for understanding the mechanisms of a major gene causing the gonad of fish and other vertebrates, including mammals, to become an ovary rather than a testis. Evidence is solid, but alternative explanations for a number of the claims must be considered and discussed. The impact of the work would benefit by placing it in a richer historical context.

Reviewed by eLife

Shc1 cooperates with Frs2 and Shp2 to recruit Grb2 in FGF-induced lens development

1. Qian Wang
2. Hongge Li
3. Yingyu Mao
4. Ankur Garg
5. Eun Sil Park
6. Yihua Wu
7. Alyssa Chow
8. John Peregrin
9. Xin Zhang

• Curated by eLife

  eLife Assessment

  This fundamental article significantly advances our understanding of FGF signalling, and in particular highlights the complex modifications affecting this pathway. The evidence for the authors' claims is convincing, combining state of the art conditional gene deletion in the mouse lens with histological and molecular approaches. This work should be of great interest to molecular and developmental biologists beyond the lens community.

Reviewed by eLife

Molecular dynamics of the matrisome across sea anemone life history

1. B Gideon Bergheim
2. Alison G Cole
3. Mandy Rettel
4. Frank Stein
5. Stefan Redl
6. Michael W Hess
7. Aissam Ikmi
8. Suat Özbek

• Curated by eLife

  eLife Assessment

  This valuable study provides a comprehensive description of the Nematostella vectensis matrisome - the genes encoding the proteins of the extracellular matrix. The authors combine new mass spectrometry data with bioinformatic analyses of previously published genomic and single-cell RNAseq data. Although this work will be of interest to biologists working on the evolution of the matrisome, as well as more broadly those working with non-bilaterian animals, in its current state it is incomplete due to the lack of rigorous criteria for manual curation and comprehensive annotation of the predicted matrisome.

Reviewed by eLife