Cell Biology

MYC overrides HIF-1α to regulate proliferating primary cell metabolism in hypoxia

1. Courtney A Copeland
2. Benjamin A Olenchock
3. David Ziehr
4. Sarah McGarrity
5. Kevin Leahy
6. Jamey D Young
7. Joseph Loscalzo
8. William M Oldham

• Curated by eLife

  eLife assessment

  The authors find a significant and unexpected consequence of hypoxia in lung fibroblasts and smooth muscle cells - decreased lactate production - a finding that is important in the field of pulmonary hypertension. Additional orthogonal assessments of lactate production will strengthen the conclusions put forward.

Reviewed by eLife

Transcriptional drifts associated with environmental changes in endothelial cells

1. Yalda Afshar
2. Feyiang Ma
3. Austin Quach
4. Anhyo Jeong
5. Hannah L Sunshine
6. Vanessa Freitas
7. Yasaman Jami-Alahmadi
8. Raphael Helaers
9. Xinmin Li
10. Matteo Pellegrini
11. James A Wohlschlegel
12. Casey E Romanoski
13. Miikka Vikkula
14. M Luisa Iruela-Arispe

• Curated by eLife

  eLife assessment’

  This paper is of interest to a broad audience of cell biologists, and researchers who work with cultured endothelial cells. The work uncovers the impact of culture conditions on transcriptional changes of endothelial cells and demonstrates that some of these changes can be recovered by sheer forces or coculture. The authors provide valuable datasets which will be a good resource for the community.

Reviewed by eLife

A role for the centrosome in regulating the rate of neuronal efferocytosis by microglia in vivo

1. Katrin Möller
2. Max Brambach
3. Ambra Villani
4. Elisa Gallo
5. Darren Gilmour
6. Francesca Peri

• Curated by eLife

  eLife assessment

  This paper is an important contribution to the microglia field and will be of interest to a broad readership in the fields of neurobiology, cell biology and immunology. This work describes fundamental mechanisms of efferocytosis by microglia and uses impressive imaging in zebrafish, in combination with molecular manipulations, to provide compelling data of how centrosome movements synchronize with phagocytic cup formation during microglial efferocytosis of neuronal corpses in vivo.

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Biological condensates form percolated networks with molecular motion properties distinctly different from dilute solutions

1. Zeyu Shen
2. Bowen Jia
3. Yang Xu
4. Jonas Wessén
5. Tanmoy Pal
6. Hue Sun Chan
7. Shengwang Du
8. Mingjie Zhang

• Curated by eLife

  eLife assessment

  The authors report results from experiments and modeling that study the motions of molecules in the dense and dilute phases of biomolecular condensates, with the key finding that molecules in the dense phase of condensates formed by folded domains appear to switch between a confined state with low apparent diffusivity and a mobile state with a high apparent diffusivity that is comparable to that of molecules in the dilute phase. The study provides experimental evidence that is suggestive of phase separation coupled with percolation as the operative mechanism that gives rise to biomolecular condensates.

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Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking

1. Xiaoqin Fu
2. Lu Rao
3. Peijun Li
4. Xinglei Liu
5. Qi Wang
6. Alexander I Son
7. Arne Gennerich
8. Judy Shih-Hwa Liu

• Curated by eLife

  eLife assessment

  In their paper, Rao, Li et al. explore the mechanisms by which the microtubule-associated protein, doublecortin (DCX), functions in regulating retrograde transport in neurons. They find that DCX affects the dynein-microtubule interaction to perturb its motion. Impressively, they reconstitute a dynein-dynactin-JIP3 complex, validating JIP3 as a bona fide adaptor, and show that DCX disrupts the transport of this processive complex. This mechanism will be useful in understanding how mutations in DCX cause lissencephaly and this paper will be of interest to those in the cytoskeletal and neurobiology fields.

Reviewed by eLife

Heterogeneous levels of delta-like 4 within a multinucleated niche cell maintains muscle stem cell diversity

1. Susan Eliazer
2. Xuefeng Sun
3. Emilie Barruet
4. Andrew S Brack

• Curated by eLife

  eLife assessment

  The premise behind this manuscript is important and timely for muscle biologists and for stem cell biologists. The identification of heterogenous distribution of factors across the myofiber is an important contribution for dissecting how muscle stem cell diversity in a tissue is achieved. However, the mechanism of action proposed by the authors will require additional experimental support.

Reviewed by eLife

Multinucleation resets human macrophages for specialized functions at the expense of their identity

1. Kourosh Ahmadzadeh
2. Marie Pereira
3. Margot Vanoppen
4. Eline Bernaerts
5. Jeong‐Hun Ko
6. Tania Mitera
7. Christy Maksoudian
8. Bella B Manshian
9. Stefaan Soenen
10. Carlos D Rose
11. Graham R Williams
12. J H Duncan Bassett
13. Patrick Matthys
14. Carine Wouters
15. Jacques Behmoaras

Reviewed by Review Commons

Regulation of store-operated Ca2+ entry by IP3 receptors independent of their ability to release Ca2+

1. Pragnya Chakraborty
2. Bipan Kumar Deb
3. Vikas Arige
4. Thasneem Musthafa
5. Sundeep Malik
6. David I Yule
7. Colin W Taylor
8. Gaiti Hasan

• Curated by eLife

  eLife assessment

  In this manuscript, Chakraborty et al address the role of IP3R1 in regulating store-operated calcium entry in neurons and neural progenitors. Long-standing observations in non-neuronal cells have shown that IP3Rs are not required for SOCE. In contrast to those findings, this manuscript determines that in neuronal cells, knockdown of IP3R1 suppresses SOCE by disrupting ER-plasma membrane contact sites. The paper supports a novel role for IP3R1 as a tether in promoting membrane contact sites which would have broad implications for a range of physiological processes including SOCE and lipid metabolism.

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Lack of peroxisomal catalase affects heat shock response in Caenorhabditis elegans

1. Marina Musa
2. Pedro A Dionisio
3. Ricardo Casqueiro
4. Ira Milosevic
5. Nuno Raimundo
6. Anita Krisko

Reviewed by Review Commons

Osteocytes regulate senescence of bone and bone marrow

1. Peng Ding
2. Chuan Gao
3. Youshui Gao
4. Delin Liu
5. Hao Li
6. Jun Xu
7. Xiaoyi Chen
8. Yigang Huang
9. Changqing Zhang
10. Minghao Zheng
11. Junjie Gao

• Curated by eLife

  eLife assessment

  This paper is of potential interest for skeletal biologist studying osteocytes and skeletal aging. Using a mouse model of partial osteocyte deletion, the authors provide new understanding on the role of osteocytes in regulating other lineage cells in bone, bone marrow, and skeletal muscle. This is an important and logically presented study that offers new insight into the biology of osteocytes. The set of data from the genetic mouse model, bone phenotypic analyses, and scRNA-seq analysis largely support the conclusion.

Reviewed by eLife