Cell Biology

Comparative analysis of actin visualization by genetically encoded probes in cultured neurons

1. Attila Ignácz
2. Domonkos Nagy-Herczeg
3. Angelika Hausser
4. Katalin Schlett

Reviewed by ASAPbio crowd review

Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing

1. Hongzhe Li
2. Sandro Bräunig
3. Parashar Dhapolar
4. Göran Karlsson
5. Stefan Lang
6. Stefan Scheding

• Curated by eLife

  Evaluation Summary:

  The manuscript by Li and coworkers characterizes sorted human non-hematopoietic bone marrow cells by scRNA-seq and predicts their lineage relationships and possible interactions with mature and immature hematopoietic cells. Transcriptionally-different stromal cell subsets are identified, and their lineage relationships, cell-cell interactions and possible specialized functions are inferred or predicted from in-silico studies, paving the way for future functional and validation studies. This resource significantly adds to the current understanding human non-hematopoietic bone marrow stromal cells and their hematopoietic regulatory functions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

The fat body cortical actin network regulates Drosophila inter-organ nutrient trafficking, signaling, and adipose cell size

1. Rupali Ugrankar-Banerjee
2. Son Tran
3. Jade Bowerman
4. Anastasiia Kovalenko
5. Blessy Paul
6. W Mike Henne

• Curated by eLife

  Evaluation Summary:

  The authors explore how the actin network in the fat body impacts nutrient uptake in multiple ways. Overall, this is an interesting study that sheds light on adipocyte cytoskeletal dynamics and it's impact on nutrient trafficking and fat body storage. The work can be further strengthened by additional validation of tools and data.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Hormone replacement therapy for postmenopausal atherosclerosis is offset by late age iron deposition

1. Tianze Xu
2. Jing Cai
3. Lei Wang
4. Li Xu
5. Hongting Zhao
6. Fudi Wang
7. Esther G Meyron-Holtz
8. Fanis Missirlis
9. Tong Qiao
10. Kuanyu Li

• Curated by eLife

  eLife assessment

  In this potentially important study, the authors attempt to explain why hormone replacement therapy with estrogen is not effective in preventing atherosclerosis in post-menopausal women by showing that iron accumulation prevents the hormone replacement therapy benefit through negative regulation of estrogen receptor expression via Mdm2-mediated proteolysis. The strength of evidence is currently incomplete as control groups are missing and there is a lack of clear-cut evidence that this effect is related to the estradiol therapy in addition to the accumulation of iron in the post-menopausal state. The general public as well as specialists might find this work to be of interest.

Reviewed by eLife

Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs

1. Ece Yildiz
2. Gaby El Alam
3. Alessia Perino
4. Antoine Jalil
5. Pierre-Damien Denechaud
6. Katharina Huber
7. Lluis Fajas
8. Johan Auwerx
9. Giovanni Sorrentino
10. Kristina Schoonjans

• Curated by eLife

  eLife assessment

  This important study reports that a high-fat diet induces biliary epithelial cell proliferation and suggests this may account for the so-called ductular reaction in advanced fatty liver disease. Convincing data support the finding that the transcription factor E2F1 is required for biliary epithelial cell proliferation in mice fed with a high-fat diet, and organoid models indicate that lipid abundance promotes glycolysis in an E2F-dependent manner. These findings are potentially of broad interest to the field of liver biology and disease.

Reviewed by eLife

Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling

1. Benjamin Barsi-Rhyne
2. Aashish Manglik
3. Mark von Zastrow

• Curated by eLife

  Evaluation Summary:

  This manuscript addresses G protein-coupled receptor signaling and proposes an additional site on Beta-arrestin2 (arrestin 3) as being responsible, in significant part, for the downregulation and likely onward signalling from endosomes of a range of GPCRs. The cell biology appears to be thoroughly carried out and data presented in a statistically appropriate manner. With some textual changes and minor experimental clarification of the route taken and molecules involved, this work will be of broad interest.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform

1. Dirk H Siepe
2. Lukas T Henneberg
3. Steven C Wilson
4. Gaelen T Hess
5. Michael C Bassik
6. Kai Zinn
7. K Christopher Garcia

• Curated by eLife

  Evaluation Summary:

  This manuscript reports the development of a proteo-genomic screening methodology to identify protein-protein interactions between secreted proteins and their cell surface receptors. The authors use a CRISPRa-based approach to overexpress membrane proteins in cells and then use magnetic cell sorting to identify receptors that bind candidate ligands. This approach leads to the identification of several novel interaction pairs that are validated biochemically, including receptor tyrosine phosphatase ligands and other interactions with implications for immune system function. The work is of interest to a wide variety of fields including biochemistry and signaling.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Depletion of Plasma Membrane PI4P by ORP5 Requires Hydrolysis by SAC1 in Acceptor Membranes

1. Colleen P. Doyle
2. Liz Timple
3. Gerald R. V. Hammond

Reviewed by ASAPbio crowd review

Consequences of PDGFRα+ fibroblast reduction in adult murine hearts

1. Jill T Kuwabara
2. Akitoshi Hara
3. Sumit Bhutada
4. Greg S Gojanovich
5. Jasmine Chen
6. Kanani Hokutan
7. Vikram Shettigar
8. Anson Y Lee
9. Lydia P DeAngelo
10. Jack R Heckl
11. Julia R Jahansooz
12. Dillon K Tacdol
13. Mark T Ziolo
14. Suneel S Apte
15. Michelle D Tallquist

• Curated by eLife

  Evaluation Summary:

  A murine genetic platform reducing fibroblast expression shows normal background indicators of cardiac structure and contractile function. Yet it shows a reduced functional compromise, on ischemic or hypertrophic challenge. This suggests its value for studies of the effect of fibrosis following normal or pathological change.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Condensation of LINE-1 is critical for retrotransposition

1. Srinjoy Sil
2. Sarah Keegan
3. Farida Ettefa
4. Lance T Denes
5. Jef D Boeke
6. Liam J Holt

Reviewed by Review Commons