Cell Biology

Osteocytes regulate senescence of bone and bone marrow

1. Peng Ding
2. Chuan Gao
3. Youshui Gao
4. Delin Liu
5. Hao Li
6. Jun Xu
7. Xiaoyi Chen
8. Yigang Huang
9. Changqing Zhang
10. Minghao Zheng
11. Junjie Gao

• Curated by eLife

  eLife assessment

  This paper is of potential interest for skeletal biologist studying osteocytes and skeletal aging. Using a mouse model of partial osteocyte deletion, the authors provide new understanding on the role of osteocytes in regulating other lineage cells in bone, bone marrow, and skeletal muscle. This is an important and logically presented study that offers new insight into the biology of osteocytes. The set of data from the genetic mouse model, bone phenotypic analyses, and scRNA-seq analysis largely support the conclusion.

Reviewed by eLife

LSD1 defines the fiber type-selective responsiveness to environmental stress in skeletal muscle

1. Hirotaka Araki
2. Shinjiro Hino
3. Kotaro Anan
4. Kanji Kuribayashi
5. Kan Etoh
6. Daiki Seko
7. Ryuta Takase
8. Kensaku Kohrogi
9. Yuko Hino
10. Yusuke Ono
11. Eiichi Araki
12. Mitsuyoshi Nakao

Reviewed by Review Commons

Synergistic stabilization of microtubules by BUB-1, HCP-1, and CLS-2 controls microtubule pausing and meiotic spindle assembly

1. Nicolas Macaisne
2. Laura Bellutti
3. Kimberley Laband
4. Frances Edwards
5. Laras Pitayu-Nugroho
6. Alison Gervais
7. Thadshagine Ganeswaran
8. Hélène Geoffroy
9. Gilliane Maton
10. Julie C Canman
11. Benjamin Lacroix
12. Julien Dumont

• Curated by eLife

  eLife assessment

  This paper on the regulation of microtubule dynamics during C. elegans meiosis will be of interest to scientists in the broad field of microtubule function in both mitosis and meiosis. The experiments are beautifully conducted and presented and generally support the conclusions of the paper. The results are interesting and add to our understanding of the control of microtubule dynamics at the kinetochore and its functional consequences for meiosis.

Reviewed by eLife

Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation

1. Katrina M Longhini
2. Michael Glotzer

Reviewed by Review Commons

Systematic analysis of membrane contact sites in Saccharomyces cerevisiae uncovers modulators of cellular lipid distribution

1. Inês Gomes Castro
2. Shawn P Shortill
3. Samantha Katarzyna Dziurdzik
4. Angela Cadou
5. Suriakarthiga Ganesan
6. Rosario Valenti
7. Yotam David
8. Michael Davey
9. Carsten Mattes
10. Ffion B Thomas
11. Reut Ester Avraham
12. Hadar Meyer
13. Amir Fadel
14. Emma J Fenech
15. Robert Ernst
16. Vanina Zaremberg
17. Tim P Levine
18. Christopher Stefan
19. Elizabeth Conibear
20. Maya Schuldiner

• Curated by eLife

  eLife Assessment:

  In this manuscript, the authors perform an extensive systematic analysis of membrane contacts sites to uncover novel proteins required for tethering organelles and modulation of membrane contacts. The authors identify over 100 new potential contact site proteins and effectors including proteins associated with the recently discovered plasma membrane-LD (pClip) and Golgi-peroxisome (GoPo) contact sites. Further, the authors identify and characterize novel lipid transport proteins associated with the pClip as well as Lec1, an ER-Lipid droplet contact site associated protein which contains a novel putative lipid binding domain and may facilitate ergosterol transport between the plasma membrane and lipid droplets.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

MND1 enables repair of two-ended DNA double-strand breaks

1. Lisa Koob
2. Anoek Friskes
3. Louise van Bergen
4. Femke M. Feringa
5. Bram van den Broek
6. Emma S. Koeleman
7. Michael Schubert
8. Vincent A. Blomen
9. Thijn R. Brummelkamp
10. Lenno Krenning
11. René H. Medema

Reviewed by ASAPbio crowd review

Long-term mitotic DNA damage promotes chromokinesin-mediated missegregation of polar chromosomes in cancer cells

1. Marco Novais-Cruz
2. António Pombinho
3. Mafalda Sousa
4. André F. Maia
5. Helder Maiato
6. Cristina Ferrás

Reviewed by Review Commons

Golgi localized Arl15 regulates cargo transport, cell adhesion and motility

1. Prerna Sharma
2. Pooja Hoovina Venkatesh
3. Neha Paddillaya
4. Nikita Shah
5. BR Rajeshwari
6. Archishman Dakua
7. Aravind Penmatsa
8. Nagaraj Balasubramanian
9. Namrata Gundiah
10. Subba Rao Gangi Setty

Reviewed by ASAPbio crowd review

CLEM Site , a software for automated phenotypic screens using light microscopy and FIB-SEM

1. José M. Serra Lleti
2. Anna M. Steyer
3. Nicole L. Schieber
4. Beate Neumann
5. Christian Tischer
6. Volker Hilsenstein
7. Mike Holtstrom
8. David Unrau
9. Robert Kirmse
10. John M. Lucocq
11. Rainer Pepperkok
12. Yannick Schwab

Reviewed by Review Commons

Identification of epigenetic modulators as determinants of nuclear size and shape

1. Andria C Schibler
2. Predrag Jevtic
3. Gianluca Pegoraro
4. Daniel L Levy
5. Tom Misteli

• Curated by eLife

  eLife Assessment:

  In this manuscript the authors describe targeted, imaging-based RNAi screens to identify novel modulators of nuclear size and shape — two traits that are diagnostic and prognostic for many human diseases including cancers. The work provides novel insights into how and what dictates nuclear morphology, further decoupling key different components of lamins, chromatin, and the nuclear envelope, but there are some notable concerns regarding the scoring approach applied in the screen and hit validation. The authors also provide new evidence that lamin A may directly bind to (modified) histone H3 and how histone H3 disease mutations impact nuclear shape; this aspect of the manuscript would benefit from a more thorough analysis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife