Cell Biology

Differential nuclear import regulates nuclear RNA inheritance following mitosis

1. Michael D. Blower
2. Wei Wang
3. Judith A. Sharp

Reviewed by Review Commons

A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation

1. Huaming Xu
2. Zhijian Li
3. Chao-Chung Kuo
4. Katrin Götz
5. Thomas Look
6. Marcelo AS de Toledo
7. Kristin Seré
8. Ivan G Costa
9. Martin Zenke

• Curated by eLife

  eLife assessment

  Authors provide valuable evidence identifying a lncRNA transcribed specifically in the pDC subtype from the +32Kb promoter region which is also the region for the enhancer for Irf8 specifically in the cDC1 subtype. With convincing methodology, they provide in-depth analysis about the possible role of lncIrf8, and its promoter region and cross-talk with Irf8 promoter to identify that it is not the lncIRF8 itself but its promoter region that is crucial for pDC and cDC1 differentiation conferring feedback inhibition of Irf8 transcription. The work will be of interest to immunologists working on immune cell development.

Reviewed by eLife

Rates of global cellular translation and transcription during cell growth and the cell cycle in fission yeast

1. Clovis Basier
2. Paul Nurse

Reviewed by Review Commons

STAT5b is a key effector of NRG-1/ERBB4-mediated cardiomyocyte growth

1. Katri Vaparanta
2. Anne Jokilammi
3. Ilkka Paatero
4. Johannes A. Merilahti
5. Juho Heliste
6. Karthik Amudhala Hemanthakumar
7. Riikka Kivelä
8. Kari Alitalo
9. Pekka Taimen
10. Klaus Elenius

Reviewed by Review Commons

Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation

1. Eshwar R Tammineni
2. Lourdes Figueroa
3. Carlo Manno
4. Disha Varma
5. Natalia Kraeva
6. Carlos A Ibarra
7. Amira Klip
8. Sheila Riazi
9. Eduardo Rios

• Curated by eLife

  eLife assessment

  Junctophilin has been traditionally known as a structural anchor to keep excitation-contraction proteins in place for healthy contractile function of skeletal muscle. Here the authors provide an interesting and important role in skeletal muscle for Junctophilin, where it translocates to the nuclei and influences gene transcription. The authors provide convincing evidence for a novel role of junctophilin beyond its structural role as a regulator of gene expression.

Reviewed by eLife

Scleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype

1. Antonion Korcari
2. Anne EC Nichols
3. Mark R Buckley
4. Alayna E Loiselle

• Curated by eLife

  eLife assessment

  This fundamental work advances our understanding of the cellular and molecular changes of the aged tendon. The evidence supporting the conclusion is convincing, using a DTR-based ScxLin cell depletion model along with state-of-art proteomic and scRNA-seq analyses. This paper is of potential interest to scientists and physicians who study the mechanisms of the tendon aging process.

Reviewed by eLife

Structures of human dynein in complex with the lissencephaly 1 protein, LIS1

1. Janice M Reimer
2. Morgan E DeSantis
3. Samara L Reck-Peterson
4. Andres E Leschziner

• Curated by eLife

  eLife assessment

  This study presents the cryo-EM structure of the dynein regulator Lis1 bound to human dynein providing important insight into how these two proteins interact. The evidence supporting the claims of the authors is overall convincing though it requires some minor re-analysis. The work will be of interest to researchers working with motor proteins and neurodevelopmental disorders as it helps rationalize how mutations in Lis1 or dynein lead to disease.

Reviewed by eLife

Myosin II regulatory light chain phosphorylation and formin availability modulate cytokinesis upon changes in carbohydrate metabolism

1. Francisco Prieto-Ruiz
2. Elisa Gómez-Gil
3. Rebeca Martín-García
4. Armando Jesús Pérez-Díaz
5. Jero Vicente-Soler
6. Alejandro Franco
7. Teresa Soto
8. Pilar Pérez
9. Marisa Madrid
10. José Cansado

• Curated by eLife

  eLife assessment

  Force generation by the myosin motor plays an important role during cell division. Myosin activity is regulated by phosphorylation, which activates myosin in animals but was thought to inactivate it in yeast. In this valuable study, the authors use a combination of convincing approaches to show that under some growth conditions, dependent on the carbon source of the growth medium, phosphorylation becomes essential for myosin function.

Reviewed by eLife

Spatacsin regulates directionality of lysosome trafficking

1. Alexandre Pierga
2. Raphaël Matusiak
3. Margaux Cauhapé
4. Julien Branchu
5. Maxime Boutry
6. Frédéric Darios

Reviewed by Review Commons

SLC25A46 localizes to sites of mitochondrial fission and fusion and loss of function variants alter the oligomerization states of MFN2 and OPA1

1. Jana Schuettpelz
2. Alexandre Janer
3. Hana Antonicka
4. Eric A. Shoubridge

Reviewed by Review Commons