Cell Biology

A mechanism of salt bridge–mediated resistance to FtsZ inhibitor PC190723 revealed by a cell-based screen

1. Ajay Kumar Sharma
2. Sakshi Mahesh Poddar
3. Joyeeta Chakraborty
4. Bhagyashri Soumya Nayak
5. Srilakshmi Kalathil
6. Nivedita Mitra
7. Pananghat Gayathri
8. Ramanujam Srinivasan

Reviewed by Review Commons

Kindlin-2 inhibits TNF/NF-κB-Caspase 8 pathway in hepatocytes to maintain liver development and function

1. Huanqing Gao
2. Yiming Zhong
3. Liang Zhou
4. Sixiong Lin
5. Xiaoting Hou
6. Zhen Ding
7. Yan Li
8. Qing Yao
9. Huiling Cao
10. Xuenong Zou
11. Di Chen
12. Xiaochun Bai
13. Guozhi Xiao

• Curated by eLife

  eLife assessment

  As shown by the authors, the focal adhesion protein, kindlin-2, plays an essential role in liver development in that its genetic inactivation leads to severe fibrosis and death in young mice. This lethality is attributed to increased liver inflammation and cell death. This work will be of interest to readers studying mechanisms of liver development and pathological fibrosis.

Reviewed by eLife

Intermittent fasting induces rapid hepatocyte proliferation to restore the hepatostat in the mouse liver

1. Abby Sarkar
2. Yinhua Jin
3. Brian C DeFelice
4. Catriona Y Logan
5. Yan Yang
6. Teni Anbarchian
7. Peng Wu
8. Maurizio Morri
9. Norma F Neff
10. Huy Nguyen
11. Eric Rulifson
12. Matthew Fish
13. Avi Gurion Kaye
14. Azalia M Martínez Jaimes
15. Roel Nusse

• Curated by eLife

  eLife assessment

  The authors report that, in the murine liver, intermittent fasting alters the homeostatic regenerative programme. This has fundamental implications for the use of murine models to study liver regeneration and cancer and highlights through a series of solid mechanistic studies the role of FGF/Wnt signalling interactions in modulating fasted associated regeneration. It opens up further questions as to why this occurs, how this is beneficial to adapting to a fasting state, and the potential for translation.

Reviewed by eLife

Reevaluation of Piezo1 as a gut RNA sensor

1. Alec R Nickolls
2. Gabrielle S O'Brien
3. Sarah Shnayder
4. Yunxiao Zhang
5. Maximilian Nagel
6. Ardem Patapoutian
7. Alexander T Chesler

• Curated by eLife

  eLife assessment

  This is an important study that resolves a controversy about a proposed molecular linkage between the fields of mechanobiology and RNA signaling. While prior research had claimed that a specific mechanosensitive ion channel in the gut responds to a specific fecal RNA, this study provides compelling evidence that the mechanosensitive ion channel does not respond to the RNA.

Reviewed by eLife

Cell-based optimisation and characterisation of genetically encoded, location-based biosensors for Cdc42 or Rac activity

1. Eike K. Mahlandt
2. Gabriel Kreider-Letterman
3. Anna O. Chertkova
4. Rafael Garcia-Mata
5. Joachim Goedhart

Reviewed by Review Commons

Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death

1. Jazlyn P Borges
2. Ragnhild SR Sætra
3. Allen Volchuk
4. Marit Bugge
5. Pascal Devant
6. Bjørnar Sporsheim
7. Bridget R Kilburn
8. Charles L Evavold
9. Jonathan C Kagan
10. Neil M Goldenberg
11. Trude Helen Flo
12. Benjamin Ethan Steinberg

• Curated by eLife

  eLife assessment

  It's been widely known that the amino acid Glycine can work as a cytoprotectant and inhibit pyroptosis-associated plasma membrane rupture. However, a long-standing question has been: how does Glycine cytoprotection work? The authors observed that Glycine treatment phenocopied deficiency of NINJ1 (a recently reported cell surface molecule critical for plasma membrane rupture), and can inhibit aggregation of NINJ1. Understanding the intrinsic mechanism by which Glycine affects NINJ1 could provide a significant advance in the cell death field.

Reviewed by eLife

Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit

1. Shoji Tane
2. Keishi Shintomi
3. Kazuhisa Kinoshita
4. Yuko Tsubota
5. Makoto M Yoshida
6. Tomoko Nishiyama
7. Tatsuya Hirano

Reviewed by Review Commons

Single-motor and multi-motor motility properties of kinesin-6 family members

1. Andrew Poulos
2. Breane G. Budaitis
3. Kristen J. Verhey

Reviewed by Review Commons

The pyruvate dehydrogenase complex regulates matrix protein phosphorylation and mitophagic selectivity

1. Panagiota Kolitsida
2. Vladimir Nolic
3. Jianwen Zhou
4. Michael Stumpe
5. Natalie M. Niemi
6. Jörn Dengjel
7. Hagai Abeliovich

Reviewed by Review Commons

A motor neuron disease-associated mutation produces non-glycosylated Seipin that induces ER stress and apoptosis by inactivating SERCA2b

1. Shunsuke Saito
2. Tokiro Ishikawa
3. Satoshi Ninagawa
4. Tetsuya Okada
5. Kazutoshi Mori

• Curated by eLife

  eLife assessment

  Seipin is a multifunctional Endoplasmic Reticulum localised protein associated with seemingly unrelated human diseases. Here the authors establish a correlation between the expression of a particular mutant form of Seipin associated in humans with motor neuron disease and altered intracellular calcium dynamics and allied proteotoxic stress. The paper is noted for the clues it provides into how these cellular defects arise and for offering a plausible, but yet unproven hypothesis for the cellular pathology that may account for the human disease phenotype.

Reviewed by eLife