Cell Biology

pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells

1. Payam E Farahani
2. Xiaoyu Yang
3. Emily V Mesev
4. Kaylan A Fomby
5. Ellen H Brumbaugh-Reed
6. Caleb J Bashor
7. Celeste M Nelson
8. Jared E Toettcher

• Curated by eLife

  eLife assessment

  This is a well-explained and potentially useful study that describes the generation and use of pYtags, recombinant proteins that, if properly used, should allow spatiotemporal monitoring of the activation of different receptor tyrosine kinases in living cells. Although this study has generated new tools to evaluate receptor localization and activation in different cells, the broad concept showing that different receptor dimers generate specific stimuli, and downstream signaling pathways, is quite limited in terms of novelty. Although it is felt that the study is technologically innovative, the analysis of receptor spatial signaling is incomplete and should be improved.

Reviewed by eLife

Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells

1. Enric Gutiérrez-Martínez
2. Susana Benet Garrabé
3. Nicolas Mateos
4. Itziar Erkizia
5. Jon Ander Nieto-Garai
6. Maier Lorizate
7. Kyra JE Borgman
8. Carlo Manzo
9. Felix Campelo
10. Nuria Izquierdo-Useros
11. Javier Martinez-Picado
12. Maria F Garcia-Parajo

• Curated by eLife

  eLife assessment

  Siglec-1 (CD169), a plasma membrane-associated sialic acid-binding lectin, has been implicated in the capture of HIV and other viruses by dendritic cells and macrophages. However, the molecular details of how HIV particles are captured by Siglec-1 are poorly understood. In this paper, the authors use advanced imaging methods to analyse the cell surface distribution of Siglec-1 on immature and mature dendritic cells to study the regulation of Siglec-1 distribution by actin and regulators of actin polymerization and to understand how virus-Siglec-1 engagement leads to virus sequestration within so-called virus containing compartments. These types of analyses have only recently become feasible with the implementation of super-resolution imaging and as yet few virus-host cell systems have been examined in detail. Thus, this study has relevance for researchers studying the engagement of HIV and many other viruses with cells, as well as researchers interested in the mechanisms regulating receptor distribution and function on cells.

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The negative adipogenesis regulator Dlk1 is transcriptionally regulated by Ifrd1 (TIS7) and translationally by its orthologue Ifrd2 (SKMc15)

1. Ilja Vietor
2. Domagoj Cikes
3. Kati Piironen
4. Theodora Vasakou
5. David Heimdörfer
6. Ronald Gstir
7. Matthias David Erlacher
8. Ivan Tancevski
9. Philipp Eller
10. Egon Demetz
11. Michael W Hess
12. Volker Kuhn
13. Gerald Degenhart
14. Jan Rozman
15. Martin Klingenspor
16. Martin Hrabe de Angelis
17. Taras Valovka
18. Lukas A Huber

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Constriction forces imposed by basement membranes regulate developmental cell migration

1. Ester Molina López
2. Anna Kabanova
3. Maria D. Martín-Bermudo

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Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy

1. Wei Li
2. Xinqi Lou
3. Yingjie Zha
4. Yinyin Qin
5. Jun Zha
6. Lei Hong
7. Zhanli Xie
8. Shudi Yang
9. Chen Wang
10. Jianzhong An
11. Zhenhao Zhang
12. Shigang Qiao

• Curated by eLife

  eLife assessment

  The precise cellular and molecular mechanisms and signaling mediators underpinning the development of cardiomyopathy and heart failure in diabetes still remains unclear. In-depth investigations of the cardiac heterogeneity and cell-to-cell interactions could be of use to reveal the pathogenesis of diabetic myocardial fibrosis and thereby identify potential targets for the treatment of cardiac myopathy and heart failure. Utilizing a mouse model as well as in-vitro studies, this manuscript demonstrates cardiac cell mapping that provides novel insights into novel drivers of intercellular communication contributing to pathological extracellular matrix remodeling during diabetic myocardial fibrosis. The work provides compelling and convincing evidence to improve the understanding the cellular and molecular mechanisms of diabetes-induced cardiac pathology.

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Insights into cargo sorting by SNX32 and its role in neurite outgrowth

1. Jini Sugatha
2. Amulya Priya
3. Prateek Raj
4. Ebsy Jaimon
5. Uma Swaminathan
6. Anju Jose
7. Thomas John Pucadyil
8. Sunando Datta

• Curated by eLife

  eLife assessment

  This manuscript presents a series of important findings about the roles of the BAR-domain containing protein SNX32 in endosomal cargo sorting and in neurite outgrowth. The authors provide convincing evidence for their claims, which will be of interest for those working not only in membrane trafficking but also for cell biologists in general with interest in neurobiology.

Reviewed by eLife

UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS

1. Holly H Black
2. Jessica L Hanson
3. Julia E Roberts
4. Shannon N Leslie
5. Will Campodonico
6. Christopher C Ebmeier
7. G Aaron Holling
8. Jian Wei Tay
9. Autumn M Matthews
10. Elizabeth Ung
11. Cristina I Lau
12. Alexandra M Whiteley

• Curated by eLife

  eLife assessment

  Black et al., provide evidence that levels of Paternally Expressed Gene 10 (PEG10) protein are regulated by Ubqln2 and that proteolytic fragments from PEG10 cleavage induce changes in gene expression, in particular genes that encode proteins involved in axon biology. Based on these data and the finding of an increase in PEG10 levels and alterations of proteins regulated by PEG10 in the spinal cord of ALS patients, they propose that abnormal induction of PEG10-regulated genes is involved in ALS. However, the evidence for these claims and PEG10 involvement could be strengthened, and the rigor of the work could be enhanced in places.

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UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation independently of the p97 ATPase

1. Brittany A. Ahlstedt
2. Rakesh Ganji
3. Sirisha Mukkavalli
4. Joao A. Paulo
5. Steve P. Gygi
6. Malavika Raman

Reviewed by Review Commons

MYSM1 co-activates ERα action via histone and non-histone deubiquitination to confer antiestrogen resistance in breast cancer

1. Ruina Luan
2. Ge Sun
3. Baosheng Zhou
4. Manlin Wang
5. Yu Bai
6. Chunyu Wang
7. Shengli Wang
8. Kai Zeng
9. Jianwei Feng
10. Mingcong He
11. Lin Lin
12. Yuntao Wei
13. Qiang Zhang
14. Yue Zhao

Reviewed by Review Commons

Continuous muscle, glial, epithelial, neuronal, and hemocyte cell lines for Drosophila research

1. Nikki Coleman-Gosser
2. Yanhui Hu
3. Shiva Raghuvanshi
4. Shane Stitzinger
5. Weihang Chen
6. Arthur Luhur
7. Daniel Mariyappa
8. Molly Josifov
9. Andrew Zelhof
10. Stephanie E Mohr
11. Norbert Perrimon
12. Amanda Simcox

• Curated by eLife

  eLife assessment

  This useful work describes the establishment and characterization of new cell lines derived from specific tissues of the fruit fly Drosophila. The evidence supporting the claims of the authors is solid, although the characterization of the cell lines is incomplete and the genomic findings are not presented in a user-friendly manner. These lines could be a useful resource that complements in vivo Drosophila genetics, improving biochemistry and facilitating high-throughput screening.

Reviewed by eLife