Cell Biology

Defects in lipid homeostasis reflect the function of TANGO2 in phospholipid and neutral lipid metabolism

1. Agustin Leonardo Lujan
2. Ombretta Foresti
3. Conor Sugden
4. Nathalie Brouwers
5. Alex Mateo Farre
6. Alessio Vignoli
7. Mahshid Azamian
8. Alicia Turner
9. Jose Wojnacki
10. Vivek Malhotra

• Curated by eLife

  eLife assessment

  This important manuscript describes a series of cellular phenotypes associated with the depletion of TANGO2, a poorly characterized gene product but relevant to neurological and muscular disorders. The authors present solid data indicating that TANGO2 associates with membrane-bound organelles, mainly mitochondria, impacting lipid metabolism and the accumulation of reactive-oxygen species. A few additional experiments would help to understand the link between the lipid changes reported and the cellular phenotype.

Reviewed by eLife

Pbp1 stabilizes and promotes the translation of Puf3-target mRNAs involved in mitochondrial biogenesis

1. Floortje van de Poll
2. Benjamin M. Sutter
3. Chien-Der Lee
4. Yu-San Yang
5. Benjamin P. Tu

Reviewed by Review Commons

The SARS-CoV-2 protein NSP2 enhances microRNA-mediated translational repression

1. Parisa Naeli
2. Xu Zhang
3. Patric Harris Snell
4. Susanta Chatterjee
5. Muhammad Kamran
6. Reese Jalal Ladak
7. Nick Orr
8. Thomas Duchaine
9. Nahum Sonenberg
10. Seyed Mehdi Jafarnejad

Reviewed by Review Commons

A protein hydroxylase couples epithelial membrane biology to nucleolar ribosome biogenesis

1. Eline Hendrix
2. Regina Andrijes
3. Uncaar Boora
4. Arashpreet Kaur
5. James R Bundred
6. Leah Officer-Jones
7. Rachel Pennie
8. Ian R Powley
9. Adam Zayer
10. Raphael Heilig
11. Christian A E Westrip
12. Sally C Fletcher
13. Charlotte D Eaton
14. Tristan J Kennedy
15. Sonia Piasecka
16. Roman Fischer
17. Stephen J Smerdon
18. John Le Quesne
19. Mathew L Coleman

Reviewed by Life Science Editors

Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system

1. Dalen Zuidema
2. Alexis Jones
3. Won-Hee Song
4. Michal Zigo
5. Peter Sutovsky

• Curated by eLife

  eLife assessment

  This important work reports the identification of a list of proteins that may participate in the clearance of paternal mitochondria during fertilization, which is known as essential for normal fertilization and embryonic and fetal development. The main method used is state-of-the-art and the supporting data are solid. This work will be of interest to developmental and reproductive biologists working on fertilization.

Reviewed by eLife

Intermediate filament network perturbation in the C. elegans intestine causes systemic dysfunctions

1. Florian Geisler
2. Sanne Remmelzwaal
3. Vera Jankowski
4. Ruben Schmidt
5. Mike Boxem
6. Rudolf E Leube

• Curated by eLife

  eLife Assessment:

  Mutations in a variety of intermediate filament proteins and their regulators lead to abnormal development, reduced lifetime, and increased stress sensitivity. This manuscript rigorously demonstrates that such defects result from inappropriate assembly of intermediate filament networks, as mutations in a central intermediate filament protein prevent assembly of both the normal network and these inappropriate assemblages and largely rescue most of the defects. This has important implications for our understanding of the assembly of intermediate filament structures and for understanding and potentially treating diseases resulting from mutations in intermediate filament protein genes.

Reviewed by eLife

Pigment cell progenitor heterogeneity and reiteration of developmental signaling underlie melanocyte regeneration in zebrafish

1. William Tyler Frantz
2. Sharanya Iyengar
3. James Neiswender
4. Alyssa Cousineau
5. René Maehr
6. Craig J Ceol

• Curated by eLife

  eLife assessment

  In this work Frantz et al. assess transcriptomic states of melanocyte stem cells that are recruited to differentiate during the process of melanocyte regeneration in zebrafish and they analyze roles for Kit signaling in this process. The analyses are nicely done, and the paper requires only relatively minor modifications and clarifications. The study will provide new insights into melanocyte stem cell biology that should be of interest to those studying pigmentation, regeneration, and melanoma biology using zebrafish and other systems.

Reviewed by eLife

Atypical peripheral actin band formation via overactivation of RhoA and nonmuscle myosin II in mitofusin 2-deficient cells

1. Yueyang Wang
2. Lee D Troughton
3. Fan Xu
4. Aritra Chatterjee
5. Chang Ding
6. Han Zhao
7. Laura Pulido Cifuentes
8. Ryan B Wagner
9. Tianqi Wang
10. Shelly Tan
11. Jingjuan Chen
12. Linlin Li
13. David Umulis
14. Shihuan Kuang
15. Daniel M Suter
16. Chongli Yuan
17. Deva Chan
18. Fang Huang
19. Patrick W Oakes
20. Qing Deng

Reviewed by Review Commons

pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells

1. Payam E Farahani
2. Xiaoyu Yang
3. Emily V Mesev
4. Kaylan A Fomby
5. Ellen H Brumbaugh-Reed
6. Caleb J Bashor
7. Celeste M Nelson
8. Jared E Toettcher

• Curated by eLife

  eLife assessment

  This is a well-explained and potentially useful study that describes the generation and use of pYtags, recombinant proteins that, if properly used, should allow spatiotemporal monitoring of the activation of different receptor tyrosine kinases in living cells. Although this study has generated new tools to evaluate receptor localization and activation in different cells, the broad concept showing that different receptor dimers generate specific stimuli, and downstream signaling pathways, is quite limited in terms of novelty. Although it is felt that the study is technologically innovative, the analysis of receptor spatial signaling is incomplete and should be improved.

Reviewed by eLife

Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells

1. Enric Gutiérrez-Martínez
2. Susana Benet Garrabé
3. Nicolas Mateos
4. Itziar Erkizia
5. Jon Ander Nieto-Garai
6. Maier Lorizate
7. Kyra JE Borgman
8. Carlo Manzo
9. Felix Campelo
10. Nuria Izquierdo-Useros
11. Javier Martinez-Picado
12. Maria F Garcia-Parajo

• Curated by eLife

  eLife assessment

  Siglec-1 (CD169), a plasma membrane-associated sialic acid-binding lectin, has been implicated in the capture of HIV and other viruses by dendritic cells and macrophages. However, the molecular details of how HIV particles are captured by Siglec-1 are poorly understood. In this paper, the authors use advanced imaging methods to analyse the cell surface distribution of Siglec-1 on immature and mature dendritic cells to study the regulation of Siglec-1 distribution by actin and regulators of actin polymerization and to understand how virus-Siglec-1 engagement leads to virus sequestration within so-called virus containing compartments. These types of analyses have only recently become feasible with the implementation of super-resolution imaging and as yet few virus-host cell systems have been examined in detail. Thus, this study has relevance for researchers studying the engagement of HIV and many other viruses with cells, as well as researchers interested in the mechanisms regulating receptor distribution and function on cells.

Reviewed by eLife