Cell Biology

Mitotic chromosomes scale to nuclear-cytoplasmic ratio and cell size in Xenopus

1. Coral Y Zhou
2. Bastiaan Dekker
3. Ziyuan Liu
4. Hilda Cabrera
5. Joel Ryan
6. Job Dekker
7. Rebecca Heald

• Curated by eLife

  eLife assessment

  This study combines experiments in developing embryos and embryo extracts to investigate a fundamental relationship in biology - how the size of mitotic chromosomes scales with changes in cell size during development. By combining the unique tools available in the Xenopus system with modern genomic approaches, the authors convincingly demonstrate that mitotic chromosome scaling is mediated by differential loading of maternal chromatin remodeling factors during interphase. Although it remains unclear exactly how these factors impact chromosome size, the findings reported here will be of broad interest to the cell biology community and are likely to spawn new avenues of experimental inquiry aimed at understanding intracellular scaling relationships.

Reviewed by eLife

The carboxyl-terminal sequence of PUMA binds to both anti-apoptotic proteins and membranes

1. James M Pemberton
2. Dang Nguyen
3. Elizabeth J Osterlund
4. Wiebke Schormann
5. Justin P Pogmore
6. Nehad Hirmiz
7. Brian Leber
8. David W Andrews

Reviewed by Review Commons

Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosure

1. Tylor R Lewis
2. Sebastien Phan
3. Carson M Castillo
4. Keun-Young Kim
5. Kelsey Coppenrath
6. William Thomas
7. Ying Hao
8. Nikolai P Skiba
9. Marko E Horb
10. Mark H Ellisman
11. Vadim Y Arshavsky

Reviewed by Review Commons

Myofibroblast senescence promotes arrhythmogenic remodeling in the aged infarcted rabbit heart

1. Brett C Baggett
2. Kevin R Murphy
3. Elif Sengun
4. Eric Mi
5. Yueming Cao
6. Nilufer N Turan
7. Yichun Lu
8. Lorraine Schofield
9. Tae Yun Kim
10. Anatoli Y Kabakov
11. Peter Bronk
12. Zhilin Qu
13. Patrizia Camelliti
14. Patrycja Dubielecka
15. Dmitry Terentyev
16. Federica del Monte
17. Bum-Rak Choi
18. John Sedivy
19. Gideon Koren

• Curated by eLife

  eLife assessment

  This study describes important results and convincing evidence linking myofibroblast senescence in the aged heart with a pro-arrhythmogenic phenotype. This is in turn related to higher mortality after myocardial infarction in the aged rabbit heart. These constitute important empiric as opposed to detailed findings. They nevertheless will be of interest to clinician scientists studying cardiac function and disease.

Reviewed by eLife

PTH regulates osteogenesis and suppresses adipogenesis through Zfp467 in a feed-forward, PTH1R-cyclic AMP-dependent manner

1. Hanghang Liu
2. Akane Wada
3. Isabella Le
4. Phuong T Le
5. Andrew WF Lee
6. Jun Zhou
7. Francesca Gori
8. Roland Baron
9. Clifford J Rosen

• Curated by eLife

  eLife assessment

  The study provides evidence that the hormone PTH increases bone mass by, at least in part, regulating the factor Zfp467. In turn, Zfp67 controls expression of the receptor for PTH, thus creating a feedback loop that overall augments bone mass. The findings are novel and of potential great interest. Overall, the study is of interest to a broad audience and significant as it unveils a novel feedback loop involving PTH, a critical endocrine regulator of calcium, phosphate, and bone mass.

Reviewed by eLife

Calaxin stabilizes the docking of outer arm dyneins onto ciliary doublet microtubule in vertebrates

1. Hiroshi Yamaguchi
2. Motohiro Morikawa
3. Masahide Kikkawa

• Curated by eLife

  eLife assessment

  In vertebrates, ciliary motility is powered by axonemal dyneins, known as OADs, tethered to doublet microtubules by a pentameric docking complex including the Armc4 and Calaxin subunits. This valuable study combines zebrafish genetics with cryo-electron tomography to convincingly show that Armc4 plays a critical role in the docking of OAD and that Calaxin stabilizes the molecular interaction. The work will be of interest to those studying the structure and function of the axoneme, and motile cilia in general.

Reviewed by eLife

Identification of Paired-related Homeobox Protein 1 as a key mesenchymal transcription factor in pulmonary fibrosis

1. Emmeline Marchal-Duval
2. Méline Homps-Legrand
3. Antoine Froidure
4. Madeleine Jaillet
5. Mada Ghanem
6. Deneuville Lou
7. Aurélien Justet
8. Arnaud Maurac
9. Aurelie Vadel
10. Emilie Fortas
11. Aurelie Cazes
12. Audrey Joannes
13. Laura Giersh
14. Herve Mal
15. Pierre Mordant
16. Tristan Piolot
17. Marin Truchin
18. Carine M Mounier
19. Ksenija Schirduan
20. Martina Korfei
21. Andreas Gunther
22. Bernard Mari
23. Frank Jaschinski
24. Bruno Crestani
25. Arnaud A Mailleux

• Curated by eLife

  eLife assessment

  This manuscript will be of interest to scientists in the field of tissue injury and repair. It provides novel molecular mechanisms of a transcription factor, Prrx1, in fibroblast activation following lung injury. Overall, the work suggests that PRRX1 plays a functional role downstream of TGFb1 to elicit some aspects of the fibrotic response and that PRRX1 could represent an important therapeutic target to treat fibrosis. The strengths of this work are the multiple approaches applying human and mouse lung tissue used by the authors to test the role of PRRX1 in lung fibrosis, however, in its current form, major limitations need to be addressed.

Reviewed by eLife

Atf3 defines a population of pulmonary endothelial cells essential for lung regeneration

1. Terren K Niethamer
2. Lillian I Levin
3. Michael P Morley
4. Apoorva Babu
5. Su Zhou
6. Edward E Morrisey

• Curated by eLife

  eLife Assessment

  The study has advanced our mechanistic understanding of lung regeneration. While the importance of regeneration of alveolar capillaries for long response to injury has been long recognized, the regulation of this process has not been well understood. Your study provides novel, comprehensive, and compelling evidence that the expression of the transcription factor Atf3 in alveolar capillary endothelial cells plays a critical role in the regeneration of alveolar capillaries following lung injury.

Reviewed by eLife

CD38 promotes hematopoietic stem cell dormancy via c-Fos

1. Liliia Ibneeva
2. Sumeet Pal Singh
3. Anupam Sinha
4. Sema Elif Eski
5. Rebekka Wehner
6. Luise Rupp
7. Juan Alberto Pérez-Valencia
8. Alexander Gerbaulet
9. Susanne Reinhardt
10. Manja Wobus
11. Malte von Bonin
12. Jaime Sancho
13. Frances Lund
14. Andreas Dahl
15. Marc Schmitz
16. Martin Bornhäuser
17. Triantafyllos Chavakis
18. Ben Wielockx
19. Tatyana Grinenko

Reviewed by Review Commons