Cell Biology

Endosomal trafficking of two-pore K+ efflux channel TWIK2 to plasmalemma mediates NLRP3 inflammasome activation and inflammatory injury

1. Long Shuang Huang
2. Mohammad Anas
3. Jingsong Xu
4. Bisheng Zhou
5. Peter T Toth
6. Yamuna Krishnan
7. Anke Di
8. Asrar B Malik

• Curated by eLife

  eLife assessment

  In this important study, Di et al., examine the mechanism by which potassium channels are activated prior to NLRP3 inflammasome activation. The main strength of the study is that it uses a combination of cell culture work and a mouse model to address the cell biology of inflammasome activation. However, certain aspects of the study including the characterization of inflammasome activation and the evidence to support the role of Rab11a in the translocation of TWIK2 are incomplete.

Reviewed by eLife

IRE1 RNase controls CD95-mediated cell death

1. Diana Pelizzari-Raymundo
2. Victoria Maltret
3. Manon Nivet
4. Raphael Pineau
5. Alexandra Papaioannou
6. Xingchen Zhou
7. Flavie Caradec
8. Sophie Martin
9. Matthieu Le Gallo
10. Tony Avril
11. Eric Chevet
12. Elodie Lafont

Reviewed by Review Commons

Reticular Adhesion Formation is Mediated by Flat Clathrin Lattices and Opposed by Fibrillar Adhesions

1. Laura Hakanpää
2. Amr Abouelezz
3. An-Sofie Lenaerts
4. Seyda Culfa
5. Michael Algie
6. Jenny Bärlund
7. Pekka Katajisto
8. Harvey T. McMahon
9. Leonardo Almeida-Souza

Reviewed by Review Commons

Defects in lipid homeostasis reflect the function of TANGO2 in phospholipid and neutral lipid metabolism

1. Agustin Leonardo Lujan
2. Ombretta Foresti
3. Conor Sugden
4. Nathalie Brouwers
5. Alex Mateo Farre
6. Alessio Vignoli
7. Mahshid Azamian
8. Alicia Turner
9. Jose Wojnacki
10. Vivek Malhotra

• Curated by eLife

  eLife assessment

  This important manuscript describes a series of cellular phenotypes associated with the depletion of TANGO2, a poorly characterized gene product but relevant to neurological and muscular disorders. The authors present solid data indicating that TANGO2 associates with membrane-bound organelles, mainly mitochondria, impacting lipid metabolism and the accumulation of reactive-oxygen species. A few additional experiments would help to understand the link between the lipid changes reported and the cellular phenotype.

Reviewed by eLife

Pbp1 stabilizes and promotes the translation of Puf3-target mRNAs involved in mitochondrial biogenesis

1. Floortje van de Poll
2. Benjamin M. Sutter
3. Chien-Der Lee
4. Yu-San Yang
5. Benjamin P. Tu

Reviewed by Review Commons

The SARS-CoV-2 protein NSP2 enhances microRNA-mediated translational repression

1. Parisa Naeli
2. Xu Zhang
3. Patric Harris Snell
4. Susanta Chatterjee
5. Muhammad Kamran
6. Reese Jalal Ladak
7. Nick Orr
8. Thomas Duchaine
9. Nahum Sonenberg
10. Seyed Mehdi Jafarnejad

Reviewed by Review Commons

A protein hydroxylase couples epithelial membrane biology to nucleolar ribosome biogenesis

1. Eline Hendrix
2. Regina Andrijes
3. Uncaar Boora
4. Arashpreet Kaur
5. James R Bundred
6. Leah Officer-Jones
7. Rachel Pennie
8. Ian R Powley
9. Adam Zayer
10. Raphael Heilig
11. Christian A E Westrip
12. Sally C Fletcher
13. Charlotte D Eaton
14. Tristan J Kennedy
15. Sonia Piasecka
16. Roman Fischer
17. Stephen J Smerdon
18. John Le Quesne
19. Mathew L Coleman

Reviewed by Life Science Editors

Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system

1. Dalen Zuidema
2. Alexis Jones
3. Won-Hee Song
4. Michal Zigo
5. Peter Sutovsky

• Curated by eLife

  eLife assessment

  This important work reports the identification of a list of proteins that may participate in the clearance of paternal mitochondria during fertilization, which is known as essential for normal fertilization and embryonic and fetal development. The main method used is state-of-the-art and the supporting data are solid. This work will be of interest to developmental and reproductive biologists working on fertilization.

Reviewed by eLife

Intermediate filament network perturbation in the C. elegans intestine causes systemic dysfunctions

1. Florian Geisler
2. Sanne Remmelzwaal
3. Vera Jankowski
4. Ruben Schmidt
5. Mike Boxem
6. Rudolf E Leube

• Curated by eLife

  eLife Assessment:

  Mutations in a variety of intermediate filament proteins and their regulators lead to abnormal development, reduced lifetime, and increased stress sensitivity. This manuscript rigorously demonstrates that such defects result from inappropriate assembly of intermediate filament networks, as mutations in a central intermediate filament protein prevent assembly of both the normal network and these inappropriate assemblages and largely rescue most of the defects. This has important implications for our understanding of the assembly of intermediate filament structures and for understanding and potentially treating diseases resulting from mutations in intermediate filament protein genes.

Reviewed by eLife

Pigment cell progenitor heterogeneity and reiteration of developmental signaling underlie melanocyte regeneration in zebrafish

1. William Tyler Frantz
2. Sharanya Iyengar
3. James Neiswender
4. Alyssa Cousineau
5. René Maehr
6. Craig J Ceol

• Curated by eLife

  eLife assessment

  In this work Frantz et al. assess transcriptomic states of melanocyte stem cells that are recruited to differentiate during the process of melanocyte regeneration in zebrafish and they analyze roles for Kit signaling in this process. The analyses are nicely done, and the paper requires only relatively minor modifications and clarifications. The study will provide new insights into melanocyte stem cell biology that should be of interest to those studying pigmentation, regeneration, and melanoma biology using zebrafish and other systems.

Reviewed by eLife