Cell Biology

Phosphorylation of tyrosine 90 in SH3 domain is a new regulatory switch controlling Src kinase

1. Lenka Koudelková
2. Markéta Pelantová
3. Zuzana Brůhová
4. Martin Sztacho
5. Vojtěch Pavlík
6. Dalibor Pánek
7. Jakub Gemperle
8. Pavel Talacko
9. Jan Brábek
10. Daniel Rösel

• Curated by eLife

  eLife assessment

  This manuscript explores the potential regulatory role of a previously unstudied phosphorylation site in the Src kinase SH3 domain. A mutant intended to mimic the phosphorylation of this site, Y90E, shows enhanced activity and transforming capacity, reduced mobility in the lipid bilayer, and a more open catalytic structure. In general, these findings are supported by compelling evidence. The paper will be of interest to biochemists and structural biologists studying new mechanisms that are capable of modulating the allosteric regulation of multi-domain protein kinases.

Reviewed by eLife

The Uso1 globular head interacts with SNAREs to maintain viability even in the absence of the coiled-coil domain

1. Ignacio Bravo-Plaza
2. Victor G Tagua
3. Herbert N Arst
4. Ana Alonso
5. Mario Pinar
6. Begoña Monterroso
7. Antonio Galindo
8. Miguel A Peñalva

• Curated by eLife

  eLife assessment

  This paper reports a detailed functional analysis of the Rab1 effector Uso1, and it provides a fundamental advance in our understanding of how ER-derived vesicles deliver their cargo. The authors provide compelling evidence that the key function of Uso1 is promoting SNARE complex formation rather than tethering vesicles as generally assumed. These insights will be of interest to cell and structural biologists who study membrane traffic.

Reviewed by eLife

Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis

1. Enrico Radaelli
2. Charles-Antoine Assenmacher
3. Jillian Verrelle
4. Esha Banerjee
5. Florence Manero
6. Salim Khiati
7. Anais Girona
8. Guillermo Lopez-Lluch
9. Placido Navas
10. Marco Spinazzi

• Curated by eLife

  eLife assessment

  This manuscript reports an important finding that spermatogenic defects in Parl KO mice, a genetic model for Leigh syndrome, may result from mitochondrial defects leading to ferroptosis. The finding, if confirmed, would be of great significance because male germ cell ferroptosis has not been well characterized. However, the criteria for determining male germ cell ferroptosis were vague, and the supporting data were inadequate.

Reviewed by eLife

Loss of Mfn1 but not Mfn2 enhances adipogenesis

1. JP Mann
2. LC Tabara
3. A Alvarez-Guaita
4. L Dong
5. A Haider
6. K Lim
7. P Tandon
8. JEN Minchin
9. S O’Rahilly
10. S Patel
11. DJ Fazakerley
12. J Prudent
13. RK Semple
14. DB Savage

Reviewed by Review Commons

Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins

1. Alaina H. Willet
2. Jun-Song Chen
3. Liping Ren
4. Kathleen L. Gould

Reviewed by Arcadia Science

An expanded palette of ATP sensors for subcellular and multiplexed imaging

1. Ayse Z. Sahan
2. Eventine Youngblood
3. Siddharth Das
4. Danielle L. Schmitt
5. Jin Zhang

Reviewed by Arcadia Science

A single-cell transcriptome atlas of pig skin characterizes anatomical positional heterogeneity

1. Qin Zou
2. Rong Yuan
3. Yu Zhang
4. Yifei Wang
5. Ting Zheng
6. Rui Shi
7. Mei Zhang
8. Yujing Li
9. Kaixin Fei
10. Ran Feng
11. Binyun Pan
12. Xinyue Zhang
13. Zhengyin Gong
14. Li Zhu
15. Guoqing Tang
16. Mingzhou Li
17. Xuewei Li
18. Yanzhi Jiang

• Curated by eLife

  eLife assessment

  This valuable manuscript provides a single-cell RNA sequencing analysis of adult pig skin from different species and anatomical regions. The evidence supporting the conclusions is compelling, with identification molecular and cellular differences in pig skin, yet the analyses are incomplete in the analysis of regional- or species-based differences.

Reviewed by eLife

Characterization of 3D organotypic epithelial tissues reveals tonsil-specific differences in tonic interferon signaling

1. Robert Jackson
2. Esha V Rajadhyaksha
3. Reid S Loeffler
4. Caitlyn E Flores
5. Koenraad Van Doorslaer

Reviewed by Review Commons

HEATR5B associates with dynein-dynactin and selectively promotes motility of AP1-bound endosomal membranes

1. Vanesa Madan
2. Lucas Albacete Albacete
3. Li Jin
4. Pietro Scaturro
5. Joseph L. Watson
6. Nadine Muschalik
7. Farida Begum
8. Jérôme Boulanger
9. Karl Bauer
10. Michael A. Kiebler
11. Emmanuel Derivery
12. Simon L. Bullock

Reviewed by Review Commons

A cleaved METTL3 potentiates the METTL3–WTAP interaction and breast cancer progression

1. Chaojun Yan
2. Jingjing Xiong
3. Zirui Zhou
4. Qifang Li
5. Chuan Gao
6. Mengyao Zhang
7. Liya Yu
8. Jinpeng Li
9. Ming-Ming Hu
10. Chen-Song Zhang
11. Cheguo Cai
12. Haojian Zhang
13. Jing Zhang

• Curated by eLife

  eLife assessment

  This study presents the valuable finding that a cleaved form of METTL3 (termed METTL3a) has an essential role in regulating the assembly of the METTL3-METTL14-WTAP complex. The evidence supporting the claims of the authors is solid, and the work will be of interest to medical biologists working on breast cancer.

Reviewed by eLife