Cell Biology

Mechanistic insights into the role of Ca ²⁺ -stimulated AMPK in the secretion of cellulases during carbon stress

1. Anmoldeep Randhawa
2. Tulika Sinha
3. Maitreyee Das
4. Olusola A. Ogunyewo
5. Kamran Jawed
6. Syed Shams Yazdani

Reviewed by Review Commons

Global analysis of contact-dependent human-to-mouse intercellular mRNA and lncRNA transfer in cell culture

1. Sandipan Dasgupta
2. Daniella Y Dayagi
3. Gal Haimovich
4. Emanuel Wyler
5. Tsviya Olender
6. Robert H Singer
7. Markus Landthaler
8. Jeffrey E Gerst

• Curated by eLife

  eLife assessment

  The authors show that tunneling nanotubes or TNTs are used by cells to transfer full-length mRNAs. The data show that as much as 1% of the endogenous mRNA are passed between cells by this procedure. The transferred mRNA affect the transcriptome of the acceptor cells thus highlighting the significance of this nanotube mediated trafficking of mRNA between cells. We appreciate the difficulty of this exercise. The strength of the presented evidence could be questioned based on technical limitations.

Reviewed by eLife

Probe-free optical chromatin deformation and measurement of differential mechanical properties in the nucleus

1. Benjamin Seelbinder
2. Susan Wagner
3. Manavi Jain
4. Elena Erben
5. Sergei Klykov
6. Iliya Dimitrov Stoev
7. Venkat Raghavan Krishnaswamy
8. Moritz Kreysing

• Curated by eLife

  eLife assessment

  Seelbinder et al. describe a new method for perturbing chromatin in living cells by local heating. Employing this approach, the authors uncover interesting behaviors that underscore the variability in the mechanical response of subnuclear domains and structures. The study is timely, and if some conceptual and technical aspects are improved, it should be of broad interest to both the cell biophysics and cell biology communities, in particular since the method can also be applied to study mechanical relationships of subcellular compartments in other cellular and multicellular systems.

Reviewed by eLife

The ATM-E6AP-MASTL axis mediates DNA damage checkpoint recovery

1. Yanqiu Li
2. Feifei Wang
3. Xin Li
4. Ling Wang
5. Zheng Yang
6. Zhongsheng You
7. Aimin Peng

• Curated by eLife

  eLife assessment

  This study reports the important finding that there appears to be a timer that monitors the repair of DNA after damage and regulates whether cells are subsequently able to enter mitosis. The authors identify proteins important for this decision and propose a mechanism supported by solid but not conclusive data. This study will be of interest to researchers in the fields of DNA damage repair and cell cycle control.

Reviewed by eLife

Altered cohesin dynamics and histone H3K9 modifications contribute to mitotic defects in the cbf11Δ lipid metabolism mutant

1. Akshay Vishwanatha
2. Jarmila Princová
3. Patrik Hohoš
4. Róbert Zach
5. Martin Převorovský

Reviewed by Review Commons

Mitotic chromosomes scale to nuclear-cytoplasmic ratio and cell size in Xenopus

1. Coral Y Zhou
2. Bastiaan Dekker
3. Ziyuan Liu
4. Hilda Cabrera
5. Joel Ryan
6. Job Dekker
7. Rebecca Heald

• Curated by eLife

  eLife assessment

  This study combines experiments in developing embryos and embryo extracts to investigate a fundamental relationship in biology - how the size of mitotic chromosomes scales with changes in cell size during development. By combining the unique tools available in the Xenopus system with modern genomic approaches, the authors convincingly demonstrate that mitotic chromosome scaling is mediated by differential loading of maternal chromatin remodeling factors during interphase. Although it remains unclear exactly how these factors impact chromosome size, the findings reported here will be of broad interest to the cell biology community and are likely to spawn new avenues of experimental inquiry aimed at understanding intracellular scaling relationships.

Reviewed by eLife

The carboxyl-terminal sequence of PUMA binds to both anti-apoptotic proteins and membranes

1. James M Pemberton
2. Dang Nguyen
3. Elizabeth J Osterlund
4. Wiebke Schormann
5. Justin P Pogmore
6. Nehad Hirmiz
7. Brian Leber
8. David W Andrews

Reviewed by Review Commons

Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosure

1. Tylor R Lewis
2. Sebastien Phan
3. Carson M Castillo
4. Keun-Young Kim
5. Kelsey Coppenrath
6. William Thomas
7. Ying Hao
8. Nikolai P Skiba
9. Marko E Horb
10. Mark H Ellisman
11. Vadim Y Arshavsky

Reviewed by Review Commons

Myofibroblast senescence promotes arrhythmogenic remodeling in the aged infarcted rabbit heart

1. Brett C Baggett
2. Kevin R Murphy
3. Elif Sengun
4. Eric Mi
5. Yueming Cao
6. Nilufer N Turan
7. Yichun Lu
8. Lorraine Schofield
9. Tae Yun Kim
10. Anatoli Y Kabakov
11. Peter Bronk
12. Zhilin Qu
13. Patrizia Camelliti
14. Patrycja Dubielecka
15. Dmitry Terentyev
16. Federica del Monte
17. Bum-Rak Choi
18. John Sedivy
19. Gideon Koren

• Curated by eLife

  eLife assessment

  This study describes important results and convincing evidence linking myofibroblast senescence in the aged heart with a pro-arrhythmogenic phenotype. This is in turn related to higher mortality after myocardial infarction in the aged rabbit heart. These constitute important empiric as opposed to detailed findings. They nevertheless will be of interest to clinician scientists studying cardiac function and disease.

Reviewed by eLife

PTH regulates osteogenesis and suppresses adipogenesis through Zfp467 in a feed-forward, PTH1R-cyclic AMP-dependent manner

1. Hanghang Liu
2. Akane Wada
3. Isabella Le
4. Phuong T Le
5. Andrew WF Lee
6. Jun Zhou
7. Francesca Gori
8. Roland Baron
9. Clifford J Rosen

• Curated by eLife

  eLife assessment

  The study provides evidence that the hormone PTH increases bone mass by, at least in part, regulating the factor Zfp467. In turn, Zfp67 controls expression of the receptor for PTH, thus creating a feedback loop that overall augments bone mass. The findings are novel and of potential great interest. Overall, the study is of interest to a broad audience and significant as it unveils a novel feedback loop involving PTH, a critical endocrine regulator of calcium, phosphate, and bone mass.

Reviewed by eLife