Cell Biology

Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosure

1. Tylor R Lewis
2. Sebastien Phan
3. Carson M Castillo
4. Keun-Young Kim
5. Kelsey Coppenrath
6. William Thomas
7. Ying Hao
8. Nikolai P Skiba
9. Marko E Horb
10. Mark H Ellisman
11. Vadim Y Arshavsky

Reviewed by Review Commons

Myofibroblast senescence promotes arrhythmogenic remodeling in the aged infarcted rabbit heart

1. Brett C Baggett
2. Kevin R Murphy
3. Elif Sengun
4. Eric Mi
5. Yueming Cao
6. Nilufer N Turan
7. Yichun Lu
8. Lorraine Schofield
9. Tae Yun Kim
10. Anatoli Y Kabakov
11. Peter Bronk
12. Zhilin Qu
13. Patrizia Camelliti
14. Patrycja Dubielecka
15. Dmitry Terentyev
16. Federica del Monte
17. Bum-Rak Choi
18. John Sedivy
19. Gideon Koren

• Curated by eLife

  eLife assessment

  This study describes important results and convincing evidence linking myofibroblast senescence in the aged heart with a pro-arrhythmogenic phenotype. This is in turn related to higher mortality after myocardial infarction in the aged rabbit heart. These constitute important empiric as opposed to detailed findings. They nevertheless will be of interest to clinician scientists studying cardiac function and disease.

Reviewed by eLife

PTH regulates osteogenesis and suppresses adipogenesis through Zfp467 in a feed-forward, PTH1R-cyclic AMP-dependent manner

1. Hanghang Liu
2. Akane Wada
3. Isabella Le
4. Phuong T Le
5. Andrew WF Lee
6. Jun Zhou
7. Francesca Gori
8. Roland Baron
9. Clifford J Rosen

• Curated by eLife

  eLife assessment

  The study provides evidence that the hormone PTH increases bone mass by, at least in part, regulating the factor Zfp467. In turn, Zfp67 controls expression of the receptor for PTH, thus creating a feedback loop that overall augments bone mass. The findings are novel and of potential great interest. Overall, the study is of interest to a broad audience and significant as it unveils a novel feedback loop involving PTH, a critical endocrine regulator of calcium, phosphate, and bone mass.

Reviewed by eLife

Calaxin stabilizes the docking of outer arm dyneins onto ciliary doublet microtubule in vertebrates

1. Hiroshi Yamaguchi
2. Motohiro Morikawa
3. Masahide Kikkawa

• Curated by eLife

  eLife assessment

  In vertebrates, ciliary motility is powered by axonemal dyneins, known as OADs, tethered to doublet microtubules by a pentameric docking complex including the Armc4 and Calaxin subunits. This valuable study combines zebrafish genetics with cryo-electron tomography to convincingly show that Armc4 plays a critical role in the docking of OAD and that Calaxin stabilizes the molecular interaction. The work will be of interest to those studying the structure and function of the axoneme, and motile cilia in general.

Reviewed by eLife

Identification of Paired-related Homeobox Protein 1 as a key mesenchymal transcription factor in pulmonary fibrosis

1. Emmeline Marchal-Duval
2. Méline Homps-Legrand
3. Antoine Froidure
4. Madeleine Jaillet
5. Mada Ghanem
6. Deneuville Lou
7. Aurélien Justet
8. Arnaud Maurac
9. Aurelie Vadel
10. Emilie Fortas
11. Aurelie Cazes
12. Audrey Joannes
13. Laura Giersh
14. Herve Mal
15. Pierre Mordant
16. Tristan Piolot
17. Marin Truchin
18. Carine M Mounier
19. Ksenija Schirduan
20. Martina Korfei
21. Andreas Gunther
22. Bernard Mari
23. Frank Jaschinski
24. Bruno Crestani
25. Arnaud A Mailleux

• Curated by eLife

  eLife assessment

  This manuscript will be of interest to scientists in the field of tissue injury and repair. It provides novel molecular mechanisms of a transcription factor, Prrx1, in fibroblast activation following lung injury. Overall, the work suggests that PRRX1 plays a functional role downstream of TGFb1 to elicit some aspects of the fibrotic response and that PRRX1 could represent an important therapeutic target to treat fibrosis. The strengths of this work are the multiple approaches applying human and mouse lung tissue used by the authors to test the role of PRRX1 in lung fibrosis, however, in its current form, major limitations need to be addressed.

Reviewed by eLife

Atf3 defines a population of pulmonary endothelial cells essential for lung regeneration

1. Terren K Niethamer
2. Lillian I Levin
3. Michael P Morley
4. Apoorva Babu
5. Su Zhou
6. Edward E Morrisey

• Curated by eLife

  eLife Assessment

  The study has advanced our mechanistic understanding of lung regeneration. While the importance of regeneration of alveolar capillaries for long response to injury has been long recognized, the regulation of this process has not been well understood. Your study provides novel, comprehensive, and compelling evidence that the expression of the transcription factor Atf3 in alveolar capillary endothelial cells plays a critical role in the regeneration of alveolar capillaries following lung injury.

Reviewed by eLife

Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression

1. Qinyu Hao
2. Minxue Liu
3. Swapna Vidhur Daulatabad
4. Saba Gaffari
5. You Jin Song
6. Rajneesh Srivastava
7. Shivang Bhaskar
8. Anurupa Moitra
9. Hazel Mangan
10. Elizabeth Tseng
11. Rachel B Gilmore
12. Susan M Frier
13. Xin Chen
14. Chengliang Wang
15. Sui Huang
16. Stormy Chamberlain
17. Hong Jin
18. Jonas Korlach
19. Brian McStay
20. Saurabh Sinha
21. Sarath Chandra Janga
22. Supriya G Prasanth
23. Kannanganattu V Prasanth

• Curated by eLife

  eLife assessment

  This study concerns an important area, that of monoallelic expression, but the study does not provide sufficient information about the candidate regulatory RNA to provide a significant advance over previous work, which should also be discussed in more detail.

Reviewed by eLife

CD38 promotes hematopoietic stem cell dormancy via c-Fos

1. Liliia Ibneeva
2. Sumeet Pal Singh
3. Anupam Sinha
4. Sema Elif Eski
5. Rebekka Wehner
6. Luise Rupp
7. Juan Alberto Pérez-Valencia
8. Alexander Gerbaulet
9. Susanne Reinhardt
10. Manja Wobus
11. Malte von Bonin
12. Jaime Sancho
13. Frances Lund
14. Andreas Dahl
15. Marc Schmitz
16. Martin Bornhäuser
17. Triantafyllos Chavakis
18. Ben Wielockx
19. Tatyana Grinenko

Reviewed by Review Commons

Lipid homeostasis is essential for a maximal ER stress response

1. Gilberto Garcia
2. Hanlin Zhang
3. Sophia Moreno
4. C Kimberly Tsui
5. Brant Michael Webster
6. Ryo Higuchi-Sanabria
7. Andrew Dillin

• Curated by eLife

  eLife assessment

  This valuable study addresses the anticipated but poorly understood interconnections between ER proteostasis and lipid metabolism. The authors discovered key metabolic enzymes required for integration of ER stress and lipid synthesis and followed up with several direct experiments that provide solid evidence for a broad conservation of the described interactions.

Reviewed by eLife

Kazrin promotes dynein/dynactin-dependent traffic from early to recycling endosomes

1. Ines Hernandez-Perez
2. Javier Rubio
3. Adrian Baumann
4. Henrique Girao
5. Miriam Ferrando
6. Elena Rebollo
7. Anna M Aragay
8. María Isabel Geli

• Curated by eLife

  eLife assessment

  Hernandez-Perez et al. perform a detailed analysis of Kazrin, a widely expressed protein that appears to be involved in many diverse cellular processes, but whose exact function is unknown. The authors employ mouse embryonic fibroblasts and biochemistry to investigate the function of Kazrin and determine that Kazrin promotes the dynein/dynactin-dependent transport of early endosomes. These findings are valuable to those in the field of intracellular transport, but the story will benefit from additional experiments to prove the main claims, or from textual modifications.

Reviewed by eLife