Cell Biology

Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy

1. Mitsuhiro Abe
2. Masataka Yanagawa
3. Michio Hiroshima
4. Toshihide Kobayashi
5. Yasushi Sako

Reviewed by Review Commons

Activation of the mitochondrial unfolded protein response regulates the formation of stress granules

1. Marta Lopez-Nieto
2. Zhaozhi Sun
3. Emily Relton
4. Rahme Safakli
5. Brian D. Freibaum
6. J Paul Taylor
7. Alessia Ruggieri
8. Ioannis Smyrnias
9. Nicolas Locker

Reviewed by Review Commons

Katanin, kinesin-13, and ataxin-2 inhibit premature interaction between maternal and paternal genomes in C. elegans zygotes

1. Elizabeth A Beath
2. Cynthia Bailey
3. Meghana Mahantesh Magadam
4. Shuyan Qiu
5. Karen L McNally
6. Francis J McNally

• Curated by eLife

  eLife assessment

  This is a valuable paper that identifies a potential challenge for embryos during fertilization: holding sperm contents in the fertilized embryos away from the oocyte meiotic spindle so that they don't get ejected into the polar body during meiotic chromosome segregation. The authors identify proteins involved in cytoplasmic streaming and maintaining the grouping of paternal organelles as being critical for this process. There remain minor weaknesses in the data presented but the paper provides solid evidence for the majority of its claims, and while the findings may pertain to a narrow audience the tools used and basic characterization shown will likely be relied upon by many in the community and therefore is of high value.

Reviewed by eLife

Mapping the human hematopoietic stem and progenitor cell hierarchy through integrated single-cell proteomics and transcriptomics

1. Benjamin Furtwängler
2. Nil Üresin
3. Sabrina Richter
4. Mikkel Bruhn Schuster
5. Despoina Barmpouri
6. Henrietta Holze
7. Anne Wenzel
8. Kirsten Grønbæk
9. Kim Theilgaard-Mönch
10. Fabian J. Theis
11. Erwin M. Schoof
12. Bo T Porse

Reviewed by Arcadia Science

Coherent Raman microscopy detects nucleolar defects through amide I peak shifts originating from β-sheets: an application to visualizing ongoing cellular senescence

1. Shigeo Ishibashi
2. Akihito Inoko
3. Yuki Oka
4. Philippe Leproux
5. Hideaki Kano

Reviewed by Arcadia Science

Cell-cycle-dependent mRNA localization in P-bodies

1. Adham Safieddine
2. Marie-Noëlle Benassy
3. Thomas Bonte
4. Floric Slimani
5. Oriane Pourcelot
6. Michel Kress
7. Michèle Ernoult-Lange
8. Maïté Courel
9. Emeline Coleno
10. Arthur Imbert
11. Antoine Laine
12. Annie Munier Godebert
13. Angelique Vinit
14. Corinne Blugeon
15. Guillaume Chevreux
16. Daniel Gautheret
17. Thomas Walter
18. Edouard Bertrand
19. Marianne Bénard
20. Dominique Weil

Reviewed by preLights

A human progeria-associated BAF-1 mutation modulates gene expression and accelerates aging in C. elegans

1. Raquel Romero-Bueno
2. Adrián Fragoso-Luna
3. Cristina Ayuso
4. Nina Mellmann
5. Alan Kavsek
6. Christian G Riedel
7. Jordan D Ward
8. Peter Askjaer

Reviewed by Review Commons

Two-way Dispatched function in Sonic hedgehog shedding and transfer to high-density lipoproteins

1. Kristina Ehring
2. Sophia Friederike Ehlers
3. Jurij Froese
4. Fabian Gude
5. Janna Puschmann
6. Kay Grobe

• Curated by eLife

  eLife assessment

  This useful manuscript presents an analysis of different factors that are required for release of the lipid-linked morphogen Shh from cellular membranes. The evidence is still incomplete, as experiments rely on over-expression of Shh in a single cell line and are sometimes of a correlative nature. The study, which otherwise confirms and extends previous findings, will be of interest to developmental biologists who work on Hedgehog signaling.

Reviewed by eLife

A genome-wide CRISPR/Cas9 screen identifies calreticulin as a selective repressor of ATF6α

1. Joanne Tung
2. Lei Huang
3. Ginto George
4. Heather P Harding
5. David Ron
6. Adriana Ordonez

• Curated by eLife

  eLife assessment

  In this important study, the authors explore ER stress signaling mediated by ATF6 using a genome-wide gene depletion screen. They find that the ER chaperone Calreticulin binds and directly represses ATF6, a new and intriguing function for Calreticulin. The evidence presented is convincing, based on CHO genetics and biochemical analysis.

Reviewed by eLife

Phosphoproteomic analysis reveals the diversity of signaling behind E rb B ‐inhibitor‐induced phenotypes

1. Katri Vaparanta
2. Zejia Song
3. Iman Farahani
4. Anne Jokilammi
5. Johannes Merilahti
6. Johanna Örling
7. Noora Virtanen
8. Pekka Haapaniemi
9. Cecilia Sahlgren
10. Klaus Elenius
11. Ilkka Paatero

Reviewed by Review Commons