Cell Biology

PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesis

1. Gang Liu
2. Yunxuan Hou
3. Xin Jin
4. Yixue Zhang
5. Chaoyue Sun
6. Chengquan Huang
7. Yujie Ren
8. Jianmin Gao
9. Xiuli Wang
10. Xiumei Jiang

• Curated by eLife

  eLife assessment

  This fundamental work significantly advances our understanding of how FOG1 nuclear localization is regulated during erythropoiesis and megakaryopoiesis, including the role of EPO and MPL/TPO signaling in this process. The authors provide compelling evidence using both K562 and CD34+ cells that heat shock cognate B (HSCB) can promote the proteasomal degradation of TACC3 to regulate the nuclear localization of FOG1, and that this function is independent of its role in iron-sulfur cluster (ISC) biogenesis. Together these data will be of interest to the fields of hematopoiesis and cell biology.

Reviewed by eLife

Novel 3D Approach to Model Non-Alcoholic Fatty Liver Disease using human Pluripotent Stem Cells

1. Carola Maria Morell
2. Samantha Grace Tilson
3. Rute Alexandra Tomaz
4. Arash Shahsavari
5. Andi Munteanu
6. Giovanni Canu
7. Brandon Tyler Wesley
8. Marion Perrin
9. Imbisaat Geti
10. Subhankar Mukhopadhyay
11. Francesca Mazzacuva
12. Paul Gissen
13. Jose Garcia-Bernardo
14. Martin Bachman
15. Casey Allison Rimland
16. Fotios Sampaziotis
17. Irina Mohorianu
18. Ludovic Vallier

• Curated by eLife

  eLife assessment

  The authors present an important study of a multi-cellular platform involving co-culturing of various hiPSC-derived hepatocyte like cells, cholangiocytes, stellate cells and macrophages to mimic the liver microenvironment. The aggregates are then treated with fatty acids and examined through transcriptomic and functional assays. The techniques and methodologically are sound, and the evidence supporting the conclusion is convincing, although more clinically relevant data demonstrating the effect of some potential pharmacological agents on the platform would serve to strengthen the study.

Reviewed by eLife

Transdifferentiation of fibroblasts into muscle cells to constitute cultured meat with tunable intramuscular fat deposition

1. Tongtong Ma
2. Ruimin Ren
3. Jianqi Lv
4. Ruipeng Yang
5. Xinyi Zheng
6. Yang Hu
7. Guiyu Zhu
8. Heng Wang

• Curated by eLife

  eLife assessment

  This study presents an important new technology for transdifferentiation of fibroblasts into muscle cells. The data and methods used for analysis were compelling. This study will have broad interest to cellular reprogramming biologists in particular as well as the general public.

Reviewed by eLife

Botulinum toxin intoxication requires retrograde transport and membrane translocation at the ER in RenVM neurons

1. Jeremy C Yeo
2. Felicia P Tay
3. Rebecca Bennion
4. Omar Loss
5. Jacquie Maignel
6. Laurent Pons
7. Keith Foster
8. Matthew Beard
9. Frederic Bard

• Curated by eLife

  eLife assessment

  In this valuable manuscript, Yeo et al. describe new methods for assessing the intracellular itinerary of Botulinum neurotoxin A (BoNT/A), a potent toxin used in clinical and cosmetic applications. The current manuscript challenges previously held views on how the catalytic portion of the toxin makes its way from the endocytic compartment to the cytosol, to meet its substrates. The approach taken is deemed innovative and the experiments are carefully performed, presenting solid evidence for some of the drawn conclusion; however, the conclusions one may draw from the experimental results are somewhat limited, as it is possible that the scope of their findings could be restricted to the specific neuron model and molecular tools that were used. This paper could be of interest to both cell biologists and physicians.

Reviewed by eLife

Partitioning to ordered membrane domains regulates the kinetics of secretory traffic

1. Ivan Castello-Serrano
2. Frederick A Heberle
3. Barbara Diaz-Rohrer
4. Rossana Ippolito
5. Carolyn R Shurer
6. Pablo Lujan
7. Felix Campelo
8. Kandice R Levental
9. Ilya Levental

• Curated by eLife

  eLife assessment

  In this important study, Castello-Serrano and colleagues describe, model and quantify the role of transmembrane domains in protein sorting in the secretory pathway, first at the ER and subsequently at the Golgi. Convincing data support the role of a cytoplasmic motif in ER exit, while further experiments are necessary to support a direct connection between the phase partitioning capability of the transmembrane regions and the sorting potential of domains at the Golgi/TGN.

Reviewed by eLife

A genetic and physiological model of renal dysfunction in Lowe syndrome

1. Navyashree A Ramesh
2. Vaishali Kataria
3. Indra Sara Lama
4. Rajan Thakur
5. Avishek Ghosh
6. Sanjeev Sharma
7. Aishwarya Venugopal
8. Anil Vasudevan
9. Raghu Padinjat

Reviewed by Review Commons

Spindle assembly checkpoint-dependent mitotic delay is required for cell division in absence of centrosomes

1. KC Farrell
2. Jennifer T Wang
3. Tim Stearns

• Curated by eLife

  eLife assessment

  This work explores how centrosomes, which function as the primary microtubule organizing center in animal cells, regulate cell division by examining the process in cells in which centrosome formation has been inhibited. The carefully conducted experiments provide convincing support for the important observation that elongated, but successful, mitosis observed in cells lacking centrosomes is due to delays in cell cycle progression.

Reviewed by eLife

The transmembrane protein Syndecan regulates stem cell nuclear properties and cell maintenance

1. Buffy L. Eldridge-Thomas
2. Jerome G. Bohere
3. Chantal Roubinet
4. Alexandre Barthelemy
5. Tamsin J. Samuels
6. Felipe Karam Teixeira
7. Golnar Kolahgar

Reviewed by Review Commons

Mecp2 fine-tunes quiescence exit by targeting nuclear receptors

1. Jun Yang
2. Shitian Zou
3. Zeyou Qiu
4. Mingqiang Lai
5. Qing Long
6. Huan Chen
7. Ping lin Lai
8. Sheng Zhang
9. Zhi Rao
10. Xiaoling Xie
11. Yan Gong
12. Anling Liu
13. Mangmang Li
14. Xiaochun Bai

• Curated by eLife

  eLife assessment

  This fundamental study provides insights into the mechanism controlling cell cycle reentry, establishing a regulatory role for Mecp2 degradation in shifting transcription from metabolic to proliferation genes during quiescence exit. The evidence, which includes experimental data from in vitro cell culture and an in vivo injury-induced liver regeneration model, is convincing but the trigger for MeCP2 degradation and how MeCP2 differentially regulates proliferation and metabolic genes remain unclear.

Reviewed by eLife

IF1 is a cold-regulated switch of ATP synthase to support thermogenesis in brown fat

1. Henver S. Brunetta
2. Anna S. Jung
3. Fernando Valdivieso-Rivera
4. Stepheny C. C. Zani
5. Joel Guerra
6. Vanessa Furino
7. Annelise Francisco
8. Marcelo Berçot
9. Pedro M. M. Moraes-Vieira
10. Susanne Keipert
11. Martin Jastroch
12. Laurent O. Martinez
13. Carlos H. Sponton
14. Roger F. Castilho
15. Marcelo A. Mori
16. Alexander Bartelt

Reviewed by Review Commons