Cell Biology

Fluorescein-based sensors to purify human α-cells for functional and transcriptomic analyses

1. Sevim Kahraman
2. Kimitaka Shibue
3. Dario F De Jesus
4. Hyunki Kim
5. Jiang Hu
6. Debasish Manna
7. Bridget Wagner
8. Amit Choudhary
9. Rohit N Kulkarni

• Curated by eLife

  eLife assessment

  The manuscript by Kahraman et al. describes the use of a fluorescent dye for purifying and analyzing human islet alpha cells. The study provides solid evidence that the alpha cells can be purified using this method and the cells remained viable and functional after culturing for several days. The significance of the study is access to a new tool that will be useful for islet biologists and researchers studying diabetes mechanisms.

Reviewed by eLife

Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance

1. Zengqi Zhao
2. Qiang Chen
3. Xiaojun Xiang
4. Weiwei Dai
5. Wei Fang
6. Kun Cui
7. Baolin Li
8. Qiangde Liu
9. Yongtao Liu
10. Yanan Shen
11. Yueru Li
12. Wei Xu
13. Kangsen Mai
14. Qinghui Ai

Reviewed by Review Commons

Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast

1. Takuro Tojima
2. Yasuyuki Suda
3. Natsuko Jin
4. Kazuo Kurokawa
5. Akihiko Nakano

Reviewed by Review Commons

Proteasome condensate formation is driven by multivalent interactions with shuttle factors and K48-linked ubiquitin chains

1. Kenrick A. Waite
2. Gabrielle Vontz
3. Stella Y. Lee
4. Jeroen Roelofs

Reviewed by preLights

Improving Split Reporters of Protein–Protein Interactions through Orthology-Based Protein Engineering

1. Louise-Marie Rakotoarison
2. Alison G. Tebo
3. Dorothea Böken
4. Stephanie Board
5. Lina El Hajji
6. Arnaud Gautier

Reviewed by Arcadia Science

The long non-coding RNA MALAT1 modulates NR4A1 expression through a downstream regulatory element in specific cancer-cell-types

1. Sara Wernig-Zorc
2. Uwe Schwartz
3. Paulina Martínez
4. Josefa Inalef
5. Francisca Pavicic
6. Pamela Ehrenfeld
7. Gernot Längst
8. Rodrigo Maldonado

Reviewed by PREreview

Harnessing Nature’s nanoSecrets: biocompatibility, biodistribution and bioactivity of extracellular vesicles derived from microalgae

1. Giorgia Adamo
2. Pamela Santonicola
3. Sabrina Picciotto
4. Paola Gargano
5. Aldo Nicosia
6. Valeria Longo
7. Noemi Aloi
8. Daniele P. Romancino
9. Angela Paterna
10. Estella Rao
11. Samuele Raccosta
12. Rosina Noto
13. Monica Salamone
14. Irene Deidda
15. Salvatore Costa
16. Caterina Di Sano
17. Giuseppina Zampi
18. Svenja Morsbach
19. Katharina Landfester
20. Paolo Colombo
21. Mingxing Wei
22. Paolo Bergese
23. Nicolas Touzet
24. Mauro Manno
25. Elia Di Schiavi
26. Antonella Bongiovanni

Reviewed by Arcadia Science

IntAct: a non-disruptive internal tagging strategy to study actin isoform organization and function

1. M.C. van Zwam
2. A. Dhar
3. W. Bosman
4. W. van Straaten
5. S. Weijers
6. E. Seta
7. B. Joosten
8. S. Palani
9. K. van den Dries

Reviewed by Review Commons, Arcadia Science

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

1. Maria Körner
2. Susanne R Meyer
3. Gabriella Marincola
4. Maximilian J Kern
5. Clemens Grimm
6. Christina Schuelein-Voelk
7. Utz Fischer
8. Kay Hofmann
9. Alexander Buchberger

Reviewed by Review Commons

Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp

1. Abigail C Neininger-Castro
2. James B Hayes
3. Zachary C Sanchez
4. Nilay Taneja
5. Aidan M Fenix
6. Satish Moparthi
7. Stéphane Vassilopoulos
8. Dylan Tyler Burnette

• Curated by eLife

  eLife assessment

  This manuscript describes a useful tool for quantitative assessment of sarcomere structures in healthy and perturbed cardiomyocytes grown in vitro. The work is solid, and the methods, data and analyses broadly support the claims with only minor weaknesses. The tool will be relevant to biologists working on and interested in obtaining quantitative information on sarcomere structure, function and development.

Reviewed by eLife