Cell Biology

Human Erbb2-induced Erk activity robustly stimulates cycling and functional remodeling of rat and human cardiomyocytes

1. Nicholas Strash
2. Sophia DeLuca
3. Geovanni L Janer Carattini
4. Soon Chul Heo
5. Ryne Gorsuch
6. Nenad Bursac

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to scientists in the field of regenerative medicine. The authors compare effects of persistent lentiviral expression of various mitogens in cardiomyocytes in vitro. Technically experiments are of a very high standard, but the data are somewhat difficult to translate to the in vivo situation. The statistical analyses would have to be robust and sufficient for the conclusions to be supported by the data.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

A low Smc flux avoids collisions and facilitates chromosome organization in Bacillus subtilis

1. Anna Anchimiuk
2. Virginia S Lioy
3. Florian Patrick Bock
4. Anita Minnen
5. Frederic Boccard
6. Stephan Gruber

• Curated by eLife

  Evaluation Summary:

  This manuscript presents some intriguing data to support the notion that B. subtilis cells have tuned a variety of parameters related to SMC loading and translocation to ensure that individual complexes do not collide. This is likely an important but poorly understood aspect of condensins/SMCs, and as such represents a valuable contribution to the field and should be of interest to a broad set of readers.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Metformin alleviates stress-induced cellular senescence of aging human adipose stromal cells and the ensuing adipocyte dysfunction

1. Laura Le Pelletier
2. Matthieu Mantecon
3. Jennifer Gorwood
4. Martine Auclair
5. Roberta Foresti
6. Roberto Motterlini
7. Mireille Laforge
8. Michael Atlan
9. Bruno Fève
10. Jacqueline Capeau
11. Claire Lagathu
12. Veronique Bereziat

Reviewed by eLife

Adiponectin Preserves Metabolic Fitness During Aging

1. Na Li
2. Zhuzhen Zhang
3. Shangang Zhao
4. Yi Zhu
5. Christy M. Gliniak
6. Lavanya Vishvanath
7. Yu A. An
8. May-yun Wang
9. Yingfeng Deng
10. Qingzhang Zhu
11. Toshiharu Onodera
12. Orhan K Oz
13. Ruth Gordillo
14. Rana K. Gupta
15. Ming Liu
16. Tamas L. Horvath
17. Vishwa Deep Dixit
18. Philipp E. Scherer

Reviewed by eLife

Restored TDCA and valine levels imitate the effects of bariatric surgery

1. Markus Quante
2. Jasper Iske
3. Timm Heinbokel
4. Bhavna N Desai
5. Hector Rodriguez Cetina Biefer
6. Yeqi Nian
7. Felix Krenzien
8. Tomohisa Matsunaga
9. Hirofumi Uehara
10. Ryoichi Maenosono
11. Haruhito Azuma
12. Johann Pratschke
13. Christine S Falk
14. Tammy Lo
15. Eric Sheu
16. Ali Tavakkoli
17. Reza Abdi
18. David Perkins
19. Maria-Luisa Alegre
20. Alexander S Banks
21. Hao Zhou
22. Abdallah Elkhal
23. Stefan G Tullius

Reviewed by eLife

The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains

1. Vladimir Zhemkov
2. Jonathon A Ditlev
3. Wan-Ru Lee
4. Mikaela Wilson
5. Jen Liou
6. Michael K Rosen
7. Ilya Bezprozvanny

Reviewed by eLife

INPP5E regulates CD3ζ enrichment at the immune synapse by phosphoinositide distribution control

1. Tzu-Yuan Chiu
2. Chien-Hui Lo
3. Yi-Hsuan Lin
4. Yun-Di Lai
5. Shan-Shan Lin
6. Ya-Tian Fang
7. Wei-Syun Huang
8. Shen-Yan Huang
9. Pei-Yuan Tsai
10. Fu-Hua Yang
11. Weng Man Chong
12. Yi-Chieh Wu
13. Hsing-Chen Tsai
14. Ya-Wen Liu
15. Chia-Lin Hsu
16. Jung-Chi Liao
17. Won-Jing Wang

Reviewed by eLife

Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein

1. Nicole R Gould
2. Katrina M Williams
3. Humberto C Joca
4. Olivia M Torre
5. James S Lyons
6. Jenna M Leser
7. Manasa P Srikanth
8. Marcus Hughes
9. Ramzi J Khairallah
10. Ricardo A Feldman
11. Christopher W Ward
12. Joseph P Stains

• Curated by eLife

  Summary: The article by Gould et al breaks new ground by demonstrating a role for lysosomal-mediated degradation in the mechanosensitive repression of Sclerostin levels in bone. Though the post-translational repression of Sclerostin has long been apparent, no one has yet unraveled the mechanisms. Therefore, this discovery is important to the skeletal biology community - both because of the findings themselves, and because the conditions/models used by this team to make these discoveries will be useful for other investigators, including their ability to manipulate and observe the rapid lysosome-dependent control of Sclerostin levels in vitro and in vivo in response to PTH or mechanical stimulation. In addition to the importance within this field, the work has broad impact on multiple levels including a) the clinical relevance for understanding and potentially treating osteoporosis and the skeletal phenotypes in individuals with lysosomal disease, and b) the mechanoregulation of lysosomal function and its relationships to crinophagy, which has implications not only for the regulation of Sclerostin, but also for other factors in and beyond the skeleton (RANKL, insulin).

  Essential revisions:

  The study is elegantly designed, clearly communicated, and rigorously conducted. However, the reviewers require additional data to support the overall conclusion on the significance of lysosome-mediated degradation of sclerostin in skeletal biology. First, it is important to elaborate on what gives the authors confidence that the inhibitors were effective and act as expected throughout the study - but especially Bafilomycin A1 and Apocynin in vivo. If BafA1 and Apocynin treatment in vivo work as expected, they should prevent the rapid load-dependent repression of Sclerostin levels (shown in Figure 1D). Second, the author's demonstration of mechanical load-dependent changes in sclerostin localization in osteocytes lysosomes in vivo by immunohistochemistry would be important to support the in vivo relevance of this pathway in the acute regulation of sclerostin levels. While the western blotting of mechanically loaded mouse ulnas showing previously-undocumented acute reductions in lysate sclerostin levels is interesting, it is unclear if these changes are caused by mechanical loading-induced lysosomal function.

  Reviewer #1 and Reviewer #2 opted to reveal their name to the authors in the decision letter after review.

Reviewed by eLife

De Novo Lipid Labeling for Comprehensive Analysis of Subcellular Distribution and Trafficking in Live Cells

1. Jun Zhang
2. Jia Nie
3. Haoran Sun
4. John-Paul Andersen
5. Yuguang Shi

• Curated by eLife

  Summary: Zhang et al. describe an interesting method to label newly synthesized lipids with fluorescent fatty acids and track their movement in cells. All reviewers agreed that this could potentially be a useful tool. However, they all raised concerns regarding the rigor of the characterization of this methodology.

Reviewed by eLife

Flagellar energetics from high-resolution imaging of beating patterns in tethered mouse sperm

1. Ashwin Nandagiri
2. Avinash Satish Gaikwad
3. David L Potter
4. Reza Nosrati
5. Julio Soria
6. Moira K O'Bryan
7. Sameer Jadhav
8. Ranganathan Prabhakar

Reviewed by eLife