Cell Biology

CTP and parS coordinate ParB partition complex dynamics and ParA-ATPase activation for ParABS-mediated DNA partitioning

1. James A Taylor
2. Yeonee Seol
3. Jagat Budhathoki
4. Keir C Neuman
5. Kiyoshi Mizuuchi

• Curated by eLife

  Evaluation Summary:

  This manuscript reports carefully executed experiments on the dynamics of ParA-ParB and ParB-ParB interactions. Two main findings are presented: a change in stoichiometry of ParA-ParB interactions upon ligand binding and ligand dependent DNA condensation by ParB. The work is solid, the conclusions are generally well supported by the data, however, the relevance of some of the findings could be established more directly.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Single-cell transcriptomics defines heterogeneity of epicardial cells and fibroblasts within the infarcted murine heart

1. Julia Hesse
2. Christoph Owenier
3. Tobias Lautwein
4. Ria Zalfen
5. Jonas F Weber
6. Zhaoping Ding
7. Christina Alter
8. Alexander Lang
9. Maria Grandoch
10. Norbert Gerdes
11. Jens W Fischer
12. Gunnar W Klau
13. Christoph Dieterich
14. Karl Köhrer
15. Jürgen Schrader

• Curated by eLife

  Evaluation Summary:

  The article by Hesse et al. defines heterogeneity of epicardial cells and fibroblasts in a murine model of cardiac injury to analyze the resulting populations through single cell RNA sequencing. Spatial confirmation of associated markers is performed using in-situ RNA hybridization. The work provides new insights into the heterogeneity of epicardial stromal and activated cardiac stromal cells post-injury.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Misoprostol treatment prevents hypoxia-induced cardiac dysfunction through a 14-3-3 and PKA regulatory motif on Bnip3

1. Matthew D. Martens
2. Nivedita Seshadri
3. Lucas Nguyen
4. Donald Chapman
5. Elizabeth S. Henson
6. Bo Xiang
7. Landon Falk
8. Arielys Mendoza
9. Sunil Rattan
10. Jared T. Field
11. Philip Kawalec
12. Spencer B. Gibson
13. Richard Keijzer
14. Ayesha Saleem
15. Grant M. Hatch
16. Christine A. Doucette
17. Jason M. Karch
18. Vernon W. Dolinsky
19. Ian M. Dixon
20. Adrian R. West
21. Christof Rampitsch
22. Joseph W. Gordon

Reviewed by Review Commons

HDLs extract lipophilic drugs from cells

1. Adi Zheng
2. Gilles Dubuis
3. Maria Georgieva
4. Carla Susana Mendes Ferreira
5. Marc Serulla
6. Maria del Carmen Conde Rubio
7. Evgeniya Trofimenko
8. Thomas Mercier
9. Laurent Decosterd
10. Christian Widmann

Reviewed by Review Commons

Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

1. Meredith H Wilson
2. Stephen C Ekker
3. Steven A Farber

• Curated by eLife

  Evaluation Summary:

  This manuscript has generated novel and useful tools to mark cytoplasmic lipid droplets and monitor their dynamics in various tissues in live animals. It will be of interest to researchers studying lipid metabolism and related human diseases.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development

1. Ricardo Viais
2. Marcos Fariña-Mosquera
3. Marina Villamor-Payà
4. Sadanori Watanabe
5. Lluís Palenzuela
6. Cristina Lacasa
7. Jens Lüders

Reviewed by Review Commons

Latrophilin GPCR signaling mediates synapse formation

1. Richard Sando
2. Thomas C Südhof

• Curated by eLife

  Evaluation Summary:

  The main finding that GPCR activity is necessary for latrophilins' role in synapse formation is both surprising and important. This work will inspire new research on compartmentalized GPCR signaling at the synapse.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity

1. Dinh Thi Nguyen
2. Thuong Manh Le
3. Tsuyoshi Hattori
4. Mika Takarada-Iemata
5. Hiroshi Ishii
6. Jureepon Roboon
7. Takashi Tamatani
8. Takayuki Kannon
9. Kazuyoshi Hosomichi
10. Atsushi Tajima
11. Shusuke Taniuchi
12. Masato Miyake
13. Seiichi Oyadomari
14. Takashi Tanaka
15. Nobuo Kato
16. Shunsuke Saito
17. Kazutoshi Mori
18. Osamu Hori

Reviewed by Review Commons

Neuronal regulated ire-1-dependent mRNA decay controls germline differentiation in Caenorhabditis elegans

1. Mor Levi-Ferber
2. Rewayd Shalash
3. Adrien Le-Thomas
4. Yehuda Salzberg
5. Maor Shurgi
6. Jennifer IC Benichou
7. Avi Ashkenazi
8. Sivan Henis-Korenblit

• Curated by eLife

  Evaluation Summary:

  The authors of this manuscript previously showed that ER stress, and in particular the ER stress sensor Ire1, regulates transdifferentiation in C. elegans, leading to the ectopic differentiation of germline cells. In this follow-up manuscript, the authors present several lines of evidence supporting the idea that Ire1 modules these effects through degrading a novel mRNA substrate flp6. The authors identify the neurons and neuromodulators that affect accumulation of abnormal germline cells. The reviewers agreed that the discovery that flp6 is a regulated Ire-1-dependent decay target in C. elegans, and the demonstration of a non-cell-autonomous effect of Ire1 activity, are novel and likely to be of interest to a broad readership. However, more evidence is required to support some of the main conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy

1. James Rae
2. Charles Ferguson
3. Nicholas Ariotti
4. Richard I. Webb
5. Han-Hao Cheng
6. James L. Mead
7. Jamie Riches
8. Dominic J.B. Hunter
9. Nick Martel
10. Joanne Baltos
11. Arthur Christopoulos
12. Nicole S. Bryce
13. Maria Lastra Cagigas
14. Sachini Fonseka
15. Edna C. Hardeman
16. Peter W. Gunning
17. Yann Gambin
18. Thomas Hall
19. Robert G. Parton

• Curated by eLife

  Summary: The manuscript by Rae et al. reports a new protocol for labeling genetically-tagged proteins of interest with heavy atom particles for visualization by electron microscopy. The optimized protocol builds on the use of the enzyme APEX2, fused to the target protein of interest. The contrast enhancement may be useful in diverse 3D EM techniques. Also, reviewers were enthusiastic about the prospects for quantitative studies, even for low-levels of endogenous expression. Semi-quantitative studies may be enabled because the new method appears to improve the proportionality of the signal such that the number of APEX2 tags in a sample correlates with the number of heavy atom particles. The apparent simplicity of the protocol raises the potential for it to become a standard in the field of EM labeling.

  Reviewer #1, Reviewer #2 and Reviewer #3 opted to reveal their name to the authors in the decision letter after review.

Reviewed by eLife