Cell Biology

Pak1 kinase controls cell shape through ribonucleoprotein granules

1. Joseph O Magliozzi
2. James B Moseley

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to cell biologists studying the signal transduction regulation of RNP granule assembly and its effects on cell growth. The work provides insight into the novel role of Pak1 kinase in the control of mRNA binding protein Sts5 and determines that Pak1 colocalizes with Processing (P) bodies during starvation and has function in P body dissolution after refeeding. Overall, the data are well presented, and support previous known findings.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Variable susceptibility of intestinal organoid–derived monolayers to SARS-CoV-2 infection

1. Kyung Ku Jang
2. Maria E. Kaczmarek
3. Simone Dallari
4. Ying-Han Chen
5. Takuya Tada
6. Jordan Axelrad
7. Nathaniel R. Landau
8. Kenneth A. Stapleford
9. Ken Cadwell

Reviewed by Rapid Reviews Infectious Diseases, ScreenIT

Ultrastructural analysis of nasopharyngeal epithelial cells from patients with SARS-CoV-2 infection

1. Karina Lidianne Alcântara Saraiva
2. Luydson Richardson Silva Vasconcelos
3. Matheus Filgueira Bezerra
4. Rodrigo Moraes Loyo Arcoverde
5. Sinval Pinto Brandão-Filho
6. Constância Flávia Junqueira Ayres
7. Regina Célia Bressan Queiroz de Figueiredo
8. Antonio Pereira-Neves

Reviewed by ScreenIT

Human airway lineages derived from pluripotent stem cells reveal the epithelial responses to SARS-CoV-2 infection

1. Ruobing Wang
2. Adam J. Hume
3. Mary Lou Beermann
4. Chantelle Simone-Roach
5. Jonathan Lindstrom-Vautrin
6. Jake Le Suer
7. Jessie Huang
8. Judith Olejnik
9. Carlos Villacorta-Martin
10. Esther Bullitt
11. Anne Hinds
12. Mahboobe Ghaedi
13. Stuart Rollins
14. Rhiannon B. Werder
15. Kristine M. Abo
16. Andrew A. Wilson
17. Elke Mühlberger
18. Darrell N. Kotton
19. Finn J. Hawkins

Reviewed by ScreenIT

ER-export and ARFRP1/AP-1–dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication

1. Guy J. Pearson
2. Harriet V. Mears
3. Malgorzata Broncel
4. Ambrosius P. Snijders
5. David L. V. Bauer
6. Jeremy G. Carlton

Reviewed by ScreenIT

Matriptase generates a tissue damage response via promoting Gq signalling, leading to RSK and DUOX activation

1. MA Jiajia
2. Claire A. Scott
3. HO Ying Na
4. Harsha Mahabaleshwar
5. Katherine S. Marsay
6. Changqing Zhang
7. Christopher K. J. Teow
8. NG Ser Sue
9. Weibin Zhang
10. Vinay Tergaonkar
11. Lynda J. Partridge
12. Sudipto Roy
13. Enrique Amaya
14. Tom J. Carney

• Curated by eLife

  Evaluation Summary:

  This manuscript unravels a detailed bipartite signaling mechanism, activation of which results in epithelial inflammation and cell motility. The paper is potentially of broad interest to cancer biologists and epithelial cell biologists. The data generated using the combination of genetic analyses, chemical inhibitors, and state-of-the-art confocal microscopy is of exceptionally high quality and supports the majority of the claims made in this paper.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development

1. Maryam Izadi
2. Eric Seemann
3. Dirk Schlobinski
4. Lukas Schwintzer
5. Britta Qualmann
6. Michael M Kessels

• Curated by eLife

  Evaluation Summary:

  This manuscript is of interest to scientists within the fields of actin cytoskeleton, cellular neurobiology and neurodevelopment. It explores how actin regulators are coordinated to trigger the formation of branches in neuronal dendritic arbor. Experiments are very well performed. Conclusions of the manuscript are convincingly supported by the results, although strict dependence of Cobl and Cobl-like in dendritic branch formation should perhaps be confirmed with additional experiments or tuned down. Results concerning the spatiotemporal relationship between the molecular players involved are more preliminary and few findings already published by the same group in previous articles should be expunged from this manuscript.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy

1. Miriam S Weber
2. Matthias Eibauer
3. Suganya Sivagurunathan
4. Thomas M Magin
5. Robert D Goldman
6. Ohad Medalia

• Curated by eLife

  Evaluation Summary:

  This work combines 2D and 3D cryo-electron microscopy to show that cellular keratin intermediate filaments have heterogenous diameter, protofilament number and protofilament arrangement. This demonstrates the challenge for future high resolution structure determination of these essential filaments as well as providing the basis for understanding how this heterogeneity facilitates their function.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

A mechanism with severing near barbed ends and annealing explains structure and dynamics of dendritic actin networks

1. Danielle Holz
2. Aaron R Hall
3. Eiji Usukura
4. Sawako Yamashiro
5. Naoki Watanabe
6. Dimitrios Vavylonis

• Curated by eLife

  Evaluation Summary:

  In this work, Holz and colleagues develop a computational stochastic model of lamellipodial actin network growth and turnover to address an unresolved important question: how do these networks remain wide enough, maintain angular order, and actually increase the filament length behind the leading edge? They compare the filament organization and rate of incorporation/detachment of actin subunits with experimental data published in the literature. A main result from this study is that frequent filament fragmentation and annealing are key events in the reorganization of branched actin networks. The paper is well written, contains very thorough and fair literature review, is accurate, well documented. The result is novel and significant.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Kinetochore-bound Mps1 regulates kinetochore–microtubule attachments via Ndc80 phosphorylation

1. Krishna K. Sarangapani
2. Lori B. Koch
3. Christian R. Nelson
4. Charles L. Asbury
5. Sue Biggins

Reviewed by Review Commons