Cell Biology

Conjunctival epithelial cells resist productive SARS-CoV-2 infection

1. Robert M. Jackson
2. Catherine F. Hatton
3. Jarmila Stremenova Spegarova
4. Maria Georgiou
5. Joseph Collin
6. Emily Stephenson
7. Bernard Verdon
8. Iram J. Haq
9. Rafiqul Hussain
10. Jonathan M. Coxhead
11. Hardeep-Singh Mudhar
12. Bart Wagner
13. Megan Hasoon
14. Tracey Davey
15. Paul Rooney
16. C.M. Anjam Khan
17. Chris Ward
18. Malcolm Brodlie
19. Muzlifah Haniffa
20. Sophie Hambleton
21. Lyle Armstrong
22. Francisco Figueiredo
23. Rachel Queen
24. Christopher J.A. Duncan
25. Majlinda Lako

Reviewed by ScreenIT

P2Y1 purinergic receptor identified as a diabetes target in a small-molecule screen to reverse circadian β-cell failure

1. Biliana Marcheva
2. Benjamin J Weidemann
3. Akihiko Taguchi
4. Mark Perelis
5. Kathryn Moynihan Ramsey
6. Marsha V Newman
7. Yumiko Kobayashi
8. Chiaki Omura
9. Jocelyn E Manning Fox
10. Haopeng Lin
11. Patrick E Macdonald
12. Joseph Bass

• Curated by eLife

  Evaluation Summary:

  Circadian disruption is widespread in our modern 24/7 society, leading to an increased prevalence of common diseases including type 2 diabetes. The authors conducted an unbiased screen for small-molecule compounds that can restore the attenuated insulin secretion from pancreatic beta cells caused by a disrupted circadian clock. They identified ivermectin and its clock-controlled target, the P2Y1 receptor, which regulate glucose-stimulated Ca2+ influx and insulin secretion in beta cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Transcriptional regulation of cyclophilin D by BMP/Smad signaling and its role in osteogenic differentiation

1. Rubens Sautchuk
2. Brianna H Kalicharan
3. Katherine Escalera-Rivera
4. Jennifer H Jonason
5. George A Porter
6. Hani A Awad
7. Roman A Eliseev

• Curated by eLife

  Evaluation Summary:

  This paper is of potential interest to investigators in the bone field. The study provides evidence of CypD regulation of osteoblast differentiation and offers new insights into it's transcriptional regulation. Overall, although the findings are compelling and have the potential to advance the field, several conclusions require additional data or clarification and there are some missed opportunities to strengthen the manuscript.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Volume growth in animal cells is cell cycle dependent and shows additive fluctuations

1. Clotilde Cadart
2. Larisa Venkova
3. Matthieu Piel
4. Marco Cosentino Lagomarsino

• Curated by eLife

  Evaluation Summary:

  The regulation of cell growth is crucial for our understanding of how cells control their size as well as how they balance cell mass and volume. While recent studies carefully measured single cell mass trajectories during the cell cycle, revealing complex growth patterns, the volume growth patterns of animal cells are poorly understood. In this interesting study, Cadart et al. now present high-precision measurements of 1700 HeLa cell growth trajectories and offering evidence for the mechanisms that regulate volume growth-rate fluctuations. This is an important demonstration of cell autonomous volume regulation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Activation of transient receptor potential vanilloid 4 is involved in pressure overload-induced cardiac hypertrophy

1. Yan Zou
2. Miaomiao Zhang
3. Qiongfeng Wu
4. Ning Zhao
5. Minwei Chen
6. Cui Yang
7. Yimei Du
8. Bing Han

• Curated by eLife

  Evaluation Summary:

  This study investigated the role of transient receptor potential vanilloid 4 (TRPV4) in pressure overload-induced cardiac hypertrophy. The expression of TPRV4 is increased in both heart failure and pressure overload-induced cardiac hypertrophy models. The TRPV4 deletion or inhibition ameliorated the hypertrophy cardiac pathology. The authors propose that TRPV4 is a potential therapeutic target for cardiac hypertrophy.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Plasma membrane damage limits replicative lifespan in yeast and induces premature senescence in human fibroblasts

1. Kojiro Suda
2. Yohsuke Moriyama
3. Nurhanani Razali
4. Yatzu Chiu
5. Yumiko Masukagami
6. Koutarou Nishimura
7. Hunter Barbee
8. Hiroshi Takase
9. Shinju Sugiyama
10. Yuta Yamazaki
11. Yoshikatsu Sato
12. Tetsuya Higashiyama
13. Yoshikazu Johmura
14. Makoto Nakanishi
15. Keiko Kono

Reviewed by Review Commons

Autophagosomes fuse to phagosomes and facilitate the degradation of apoptotic cells in Caenorhabditis elegans

1. Omar Peña-Ramos
2. Lucia Chiao
3. Xianghua Liu
4. Xiaomeng Yu
5. Tianyou Yao
6. Henry He
7. Zheng Zhou

• Curated by eLife

  Evaluation Summary:

  This study presents evidence that autophagosomes fuse with phagosomes and that this promotes the degradation of phagocytosed cell corpses. The study also resolves controversy in the field about the question why genes involved in autophagy affect cell corpse engulfment and degradation. With some additional data to solidify the main conclusions, the work will be of interest to a broad cell biology audience.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling

1. Caitlin E. O’Brien
2. Susan H. Younger
3. Lily Yeh Jan
4. Yuh Nung Jan

Reviewed by Review Commons

Structural basis for cytoplasmic dynein-1 regulation by Lis1

1. John P Gillies
2. Janice M Reimer
3. Eva P Karasmanis
4. Indrajit Lahiri
5. Zaw Min Htet
6. Andres E Leschziner
7. Samara L Reck-Peterson

• Curated by eLife

  Evaluation Summary:

  LIS1 is a key regulator of the microtubule motor cytoplasmic dynein. Here the authors use yeast proteins and streptavidin-coated grids to solve the first high-resolution (3.1Å) structure of the dynein-Lis1 complex. The two beta-propellors in a Lis1 dimer make contact with different sites on a single dynein motor domain. Mutagenesis shows both sites are important for yeast and human dynein and uncover how they modulate motor function.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Load adaptation of endocytic actin networks

1. Charlotte Kaplan
2. Sam J. Kenny
3. Xuyan Chen
4. Johannes Schöneberg
5. Ewa Sitarska
6. Alba Diz-Muñoz
7. Matthew Akamatsu
8. Ke Xu
9. David G. Drubin

Reviewed by ASAPbio crowd review