Cell Biology

De novo apical domain formation inside the Drosophila adult midgut epithelium

1. Jia Chen
2. Daniel St Johnston

• Curated by eLife

  Evaluation Summary:

  This paper addresses a fundamental cell biological question - the de-novo development of an apical membrane during the integration of an initially unpolarized cell, the enterocyst, into an an existing epithelium, the Drosophila midgut. The data will be of interest to a wide range of researchers including those in the fields of cell, development, stem cell and cancer biology.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Efficient Neutralization of SARS-CoV-2 Omicron and Other VOCs by a Broad Spectrum Antibody 8G3

1. Hang Ma
2. Chien-Te K. Tseng
3. Huifang Zong
4. Yunji Liao
5. Yong Ke
6. Haoneng Tang
7. Lei Wang
8. Zhenyu Wang
9. Yang He
10. Yunsong Chang
11. Shusheng Wang
12. Aleksandra Drelich
13. Jason Hsu
14. Vivian Tat
15. Yunsheng Yuan
16. Mingyuan Wu
17. Junjun Liu
18. Yali Yue
19. Wenbo Xu
20. Xiaoju Zhang
21. Ziqi Wang
22. Li Yang
23. Hua Chen
24. Yanlin Bian
25. Baohong Zhang
26. Haiyang Yin
27. Yi Chen
28. En Zhang
29. Xiaoxiao Zhang
30. John Gilly
31. Tao Sun
32. Lei Han
33. Yueqing Xie
34. Hua Jiang
35. Jianwei Zhu

Reviewed by ScreenIT

The VINE complex is an endosomal VPS9-domain GEF and SNX-BAR coat

1. Shawn P Shortill
2. Mia S Frier
3. Ponthakorn Wongsangaroonsri
4. Michael Davey
5. Elizabeth Conibear

• Curated by eLife

  Evaluation Summary:

  The SNX-BAR family of sorting nexins are a diverse group of dimeric proteins that form tubules from endosomal membranes where they also select protein cargoes for incorporation into these transport carriers. The authors of this manuscript describe a new SNX-BAR complex in budding yeast, named the VINE complex, that uniquely harbors a guanine nucleotide exchange factor (GEF) domain for Rab5-related small GTP-binding proteins. The author's data implicate VINE in endosomal membrane remodeling events and the sorting of a vacuolar hydrolase receptor. The paper would be strengthened by some additional quantifications and by taking advantage of new modeling opportunities.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

The TFIIH complex is required to establish and maintain mitotic chromosome structure

1. Julian Haase
2. Richard Chen
3. Wesley M Parker
4. Mary Kate Bonner
5. Lisa M Jenkins
6. Alexander E Kelly

• Curated by eLife

  Evaluation Summary:

  This paper reports the surprising observation that the general transcription factor TFIIH, but not transcription, is required for chromosome condensation in frog egg extracts. TFIIH may act by facilitating condensin localization and function. This opens up a lot of interesting new questions and lines of research that promise to add significantly to the field of chromosome biology. It will now be interesting to directly test the mechanism of action, and to examine whether this role of TFIIH extends to somatic cells and other animals.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Genetically controlled mtDNA deletions prevent ROS damage by arresting oxidative phosphorylation

1. Simon Stenberg
2. Jing Li
3. Arne B Gjuvsland
4. Karl Persson
5. Erik Demitz-Helin
6. Carles González Peña
7. Jia-Xing Yue
8. Ciaran Gilchrist
9. Timmy Ärengård
10. Payam Ghiaci
11. Lisa Larsson-Berglund
12. Martin Zackrisson
13. Silvana Smits
14. Johan Hallin
15. Johanna L Höög
16. Mikael Molin
17. Gianni Liti
18. Stig W Omholt
19. Jonas Warringer

• Curated by eLife

  Evaluation Summary:

  The authors report that upon exposure of yeast cells to paraquat-induced superoxide production, specific mitochondrial DNA genes encoding electron transport chain proteins are deleted to minimize the generation of endogenous superoxide. Reversible loss of mitochondrial DNA as an adaptive response to paraquat stress is an interesting idea. The data presented appear to support the proposed model, but could be further strengthened as alternative interpretations of the described observations are possible.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Mechanical stretch regulates macropinocytosis in Hydra vulgaris

1. Taylor D. Skokan
2. Bert Hobmayer
3. Kara L. McKinley
4. Ronald D. Vale

Reviewed by Review Commons, preLights

Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells

1. Nuria Vilaplana-Lopera
2. Vincent Cuminetti
3. Ruba Almaghrabi
4. Grigorios Papatzikas
5. Ashok Kumar Rout
6. Mark Jeeves
7. Elena González
8. Yara Alyahyawi
9. Alan Cunningham
10. Ayşegül Erdem
11. Frank Schnütgen
12. Manoj Raghavan
13. Sandeep Potluri
14. Jean-Baptiste Cazier
15. Jan Jacob Schuringa
16. Michelle AC Reed
17. Lorena Arranz
18. Ulrich L Günther
19. Paloma Garcia

• Curated by eLife

  Evaluation Summary:

  The authors demonstrate a molecular mechanism responsible for the rewiring of stroma cells that makes them supportive of acute myeloid leukaemia (AML) cells. Understanding metabolic interactions between leukaemia cells and the bone marrow microenvironment may provide new targets for leukaemia treatments. It has already been shown that the inhibition of gap junctions reduces AML growth in vivo, and here the authors provide evidence for a potential mechanism explaining that finding. The work will be very interesting for those working in the fields of hematopoiesis, leukemia (especially AML), cancer metabolism and cancer microenvironment.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Cortical microtubule pulling forces contribute to the union of the parental genomes in the Caenorhabditis elegans zygote

1. Griselda Velez-Aguilera
2. Batool Ossareh-Nazari
3. Lucie Van Hove
4. Nicolas Joly
5. Lionel Pintard

• Curated by eLife

  Evaluation Summary:

  This manuscript would be interesting for cell biologists and biophysicists studying nuclear organization and mechanics. The work provides new insights into how pulling forces from the cell cortex influence the dynamics of nuclear rupture during mitosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

TUBA1A tubulinopathy mutants disrupt neuron morphogenesis and override XMAP215/Stu2 regulation of microtubule dynamics

1. Katelyn J Hoff
2. Jayne E Aiken
3. Mark A Gutierrez
4. Santos J Franco
5. Jeffrey K Moore

• Curated by eLife

  Evaluation Summary:

  Tubulin mutations underlie a number of neurodevelopmental diseases, but their effect on microtubule function remains largely unknown. Using a combination of approaches and model systems, the authors provide evidence that the disease-associated alpha-tubulin mutations V409A and V409I may perturb microtubule polymerization. In addition, the mutations in alpha tubulin directly impact Tumor Overexpressed Gene domain proteins from recruiting tubulins to regulate microtubule dynamics. This study demonstrates a link between regulators of microtubule dynamics and disease pathogenesis. It will be of interest to the cytoskeleton and neurobiology fields.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Tom70-based transcriptional regulation of mitochondrial biogenesis and aging

1. Qingqing Liu
2. Catherine E Chang
3. Alexandra C Wooldredge
4. Benjamin Fong
5. Brian K Kennedy
6. Chuankai Zhou

• Curated by eLife

  Evaluation Summary:

  The authors test the hypothesis that components of the TOM complex regulate efficient mitochondrial biogenesis by coordinating the synthesis (via controlling transcription of the corresponding RNAs) of mitochondrial proteins with the rate of mitochondrial protein import. It has previously been established that failure to import mitochondrial proteins results in the accumulation of toxic protein aggregates in the cytosol. The authors conclude that Tom70 fulfills this role and find that Tom70 expression declines as cells age, which contributes to age-associated mitochondrial dysfunction.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife