Cell Biology

Adaptor linked K63 di-ubiquitin activates Nedd4/Rsp5 E3 ligase

1. Lu Zhu
2. Qing Zhang
3. Ciro D Cordeiro
4. Sudeep Banjade
5. Richa Sardana
6. Yuxin Mao
7. Scott D Emr

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to researchers in the field of protein quality control, as it describes a mechanism for regulating the activity of NEDD4 E3 ubiquitin ligases. The data largely support the main conclusions of the paper.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus

1. Natasha M Puri
2. Giovanna R Romano
3. Ting-Yu Lin
4. Quynh N Mai
5. Roshanak Irannejad

• Curated by eLife

  Evaluation Summary:

  This study uses a tour de force of biosensor constructs providing evidence that dopamine transport by OCT2 across the plasma membrane and also (presumably) into the Golgi activates GPCR signaling at the Golgi leading to cAMP production and PKA activation. Thus, intracellularly compartmentalized signaling underlies aspects of Dopamine D1 receptor signaling. The work will be of interest to scientists working on the cell biology of dopamine signaling. While the data support the model overall, there are concerns that need to be addressed including specificity of the reagents used and the actual intracellular localization of D1DR and OCT2.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Empirical single-cell tracking and cell-fate simulation reveal dual roles of p53 in tumor suppression

1. Ann Rancourt
2. Sachiko Sato
3. Masahiko S Satoh

• Curated by eLife

  Evaluation Summary:

  This paper examines the role of p53 in cell division by using a combination of live-cell imaging, cell tracking, and simulations. Overall, the results are extensively and transparently documented, and are of interest to cell biologists studying cell division, cell death, and p53.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

A tRNA processing enzyme is a key regulator of the mitochondrial unfolded protein response

1. James P Held
2. Gaomin Feng
3. Benjamin R Saunders
4. Claudia V Pereira
5. Kristopher Burkewitz
6. Maulik R Patel

• Curated by eLife

  Evaluation Summary:

  This manuscript reports that tRNA processing enzyme HOE-1 is required for the activation of mitochondrial unfolded protein response in C. elegans. This study extends our understanding of how the mitochondria-nuclear communication is mediated via a tRNA processing enzyme, and can serve as a staring point to elucidate the mechanism by which HOE-1 regulates mitochondrial unfolded protein response.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Live imaging of the co-translational recruitment of XBP1 mRNA to the ER and its processing by diffuse, non-polarized IRE1α

1. Silvia Gómez-Puerta
2. Roberto Ferrero
3. Tobias Hochstoeger
4. Ivan Zubiri
5. Jeffrey Chao
6. Tomás Aragón
7. Franka Voigt

• Curated by eLife

  Evaluation Summary:

  This study takes on a lingering question in the study of the endoplasmic reticulum unfolded protein response (UPR), namely the relationship between the oligomeric state and activity of the UPR transducer IRE1. Applying modern imaging tools to cultured mammalian cells the authors conclude that much of IRE1's effector function (the unconventional 'splicing' of the XBP1 mRNA) is carried out by finely dispersed IRE1 molecules and not by large clusters. Whilst some of the analytical tools used here remain to be fully validated, the study is likely to be of interest to students of the UPR and its timeliness is highlighted by a recent posting on BioRxiv addressing the same question (Belyy et al., 2021 DOI: 10.1101/2021.09.29.462487)

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

  This manuscript was co-submitted with: https://www.biorxiv.org/content/10.1101/2021.09.29.462487v1

Reviewed by eLife

Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion

1. Dillon Jevon
2. Kylie Deng
3. Nicole Hallahan
4. Krish Kumar
5. Jason Tong
6. Wan Jun Gan
7. Clara Tran
8. Marcela Bilek
9. Peter Thorn

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest not only to the field of insulin release and beta cell biology but also of general interest to the community interested in peptide hormone secretion. It shows that the preservation of the tissue context, in particular the local interaction with integrins at the capillary interface, is important in preserving cell function when using cultured cell or organ isolates in vitro.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Evolutionary conservation of centriole rotational asymmetry in the human centrosome

1. Noémie Gaudin
2. Paula Martin Gil
3. Meriem Boumendjel
4. Dmitry Ershov
5. Catherine Pioche-Durieu
6. Manon Bouix
7. Quentin Delobelle
8. Lucia Maniscalco
9. Than Bich Ngan Phan
10. Vincent Heyer
11. Bernardo Reina-San-Martin
12. Juliette Azimzadeh

• Curated by eLife

  Evaluation Summary:

  This paper shows that centrioles of the human centrosome are rotationally asymmetric, a feature previously known only from centrioles in flagellated protists and in multi-ciliated cells. The authors, identify LRRCC1, implicated in human ciliopathy, as a component that localizes asymmetrically on one side of the distal lumen, contributing to proper centriole structure, ciliary assembly and ciliary signaling. This work is of interest to cell biologists studying how centriole assembly and function is linked to human disease.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

A collection of yeast cellular electron cryotomography data

1. Lu Gan
2. Cai Tong Ng
3. Chen Chen
4. Shujun Cai

Reviewed by GigaScience

Morphogenetic forces planar polarize LGN/Pins in the embryonic head during Drosophila gastrulation

1. Jaclyn Camuglia
2. Soline Chanet
3. Adam C Martin

Reviewed by Review Commons

MAF1, a repressor of RNA polymerase III-dependent transcription, regulates bone mass

1. Ellen Phillips
2. Naseer Ahmad
3. Li Sun
4. James Iben
5. Christopher J Walkey
6. Aleksandra Rusin
7. Tony Yuen
8. Clifford J Rosen
9. Ian M Willis
10. Mone Zaidi
11. Deborah L Johnson

• Curated by eLife

  Evaluation Summary:

  Johnson et al. have used several complementary in vivo and in vitro approaches to analyze the effects of regulated MAF1 expression or inhibition of RNA pol III transcription on osteogenesis and adipocyte differentiation. The data are well controlled and of excellent quality, providing novel insights into Maf1 and RNA polymerase-mediated transcriptions in skeleton biology.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife