Cell Biology

The SARS-CoV-2 Spike Protein Activates the Epidermal Growth Factor Receptor-Mediated Signaling

1. Abdulrasheed Palakkott
2. Aysha Alneyadi
3. Khalid Muhammad
4. Ali H. Eid
5. Khaled Amiri
6. Mohammed Akli Ayoub
7. Rabah Iratni

Reviewed by ScreenIT

Multiple ciliary localization signals control INPP5E ciliary targeting

1. Dario Cilleros-Rodriguez
2. Raquel Martin-Morales
3. Pablo Barbeito
4. Abhijit Deb Roy
5. Abdelhalim Loukil
6. Belen Sierra-Rodero
7. Gonzalo Herranz
8. Olatz Pampliega
9. Modesto Redrejo-Rodriguez
10. Sarah C Goetz
11. Manuel Izquierdo
12. Takanari Inoue
13. Francesc R Garcia-Gonzalo

• Curated by eLife

  Evaluation Summary:

  This manuscript is of interest to developmental and cell biologists exploring cilia dynamics and ciliopathies. The authors address the molecular mechanisms by which INPP5E, a phosphoinositide phosphatase essential for regulating cilia function, is targeted to the primary cilium of cultured mammalian cells. Using immunoprecipitation, ciliary localization, phosphatase activity assays in combination with structural modelling, the authors identify four motifs important for ciliary localization of INPP5E, and uncover several novel and important interactions with other ciliary proteins providing a likely mechanism for ciliary targeting. The claims are generally well supported by the data, but some additional data acquisition and analysis are required to fully support the authors' conclusions and provide a conceptual advance in the field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene

1. Zhichao Zheng
2. Lihong Wu
3. Zhicong Li
4. Ruoshu Tang
5. Hongtao Li
6. Yinyin Huang
7. Tianqi Wang
8. Shaofen Xu
9. Haoyu Cheng
10. Zhitong Ye
11. Dong Xiao
12. Xiaolin Lin
13. Gang Wu
14. Richard T Jaspers
15. Janak L Pathak

• Curated by eLife

  Evaluation Summary:

  Caner/cancer metastasis-induced bone loss-mediated fracture is a serious clinical problem. In this regard the authors have done an interesting study to show how miR155 exhibits a catabolic effect on osteogenesis and bone mass phenotype via interaction with the Sphingosine 1-phosphate receptor-1 (S1PR1) gene. The study suggests that inhibition of miR155 could be a potential strategy for bone regeneration and bone defect healing.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL

1. Thomas L. Steinacker
2. Siu-Shing Wong
3. Zsofia A. Novak
4. Saroj Saurya
5. Lisa Gartenmann
6. Eline J.H. van Houtum
7. Judith R. Sayers
8. B. Christoffer Lagerholm
9. Jordan W. Raff

Reviewed by Review Commons

Actin network architecture can ensure robust centering or sensitive decentering of the centrosome

1. Shohei Yamamoto
2. Jérémie Gaillard
3. Benoit Vianay
4. Christophe Guerin
5. Magali Orhant‐Prioux
6. Laurent Blanchoin
7. Manuel Théry

Reviewed by Review Commons

Novel ACE2 nanoparticles universally block SARS-CoV-2 variants in the human respiratory tract

1. Cécile Sauvanet
2. Moara Lemos
3. Armel Bezault
4. Borja Rodríguez de Francisco
5. Michael CW Chan
6. Kenrie PY Hui
7. Ka-chun Ng
8. John M Nicholls
9. Niels Volkmann
10. Dorit Hanein

Reviewed by ScreenIT

High-resolution secretory timeline from vesicle formation at the Golgi to fusion at the plasma membrane in S. cerevisiae

1. Robert M Gingras
2. Abigail M Sulpizio
3. Joelle Park
4. Anthony Bretscher

• Curated by eLife

  Evaluation Summary:

  The process of secretory vesicle formation, transport, and fusion in yeast has mainly been characterized through biochemical and genetic means. Only limited information was available about the detailed timeline and order of events. This study fills the gap with a high-resolution temporal analysis, which provides new insights into when key components arrive and depart and how they promote vesicle tethering and fusion. The work is experimentally strong, and improvements to the presentation will ensure that the findings are communicated effectively.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Allelic gene polymorphisms suspected to diversify the individual early metabolic response upon influenza H3N2 and SARS-CoV-2 infections

1. Birgit Arnholdt-Schmitt
2. Shahid Aziz
3. José Hélio Costa

Reviewed by ScreenIT

CXCR4high megakaryocytes regulate host-defense immunity against bacterial pathogens

1. Jin Wang
2. Jiayi Xie
3. Daosong Wang
4. Xue Han
5. Minqi Chen
6. Guojun Shi
7. Linjia Jiang
8. Meng Zhao

• Curated by eLife

  Evaluation Summary:

  The manuscript studies the heterogeneity of megakaryocytes using single cell RNA-seq and identifies a subpopulation of CXCR4-high megakaryocytes with immune modulatory roles. The authors also perform functional studies which show that this subpopulation of megakaryocytes promotes bacterial phagocytosis by macrophages and neutrophils. This work would be of significant interest to researchers in the fields of immunology and host defense as well as researchers studying hematopoiesis and megakaryocyte biology because it provides new perspectives on megakaryocyte heterogeneity as well as the role of megakaryocytes in host defense and immune function.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Functional requirements for a Samd14-capping protein complex in stress erythropoiesis

1. Suhita Ray
2. Linda Chee
3. Yichao Zhou
4. Meg A Schaefer
5. Michael J Naldrett
6. Sophie Alvarez
7. Nicholas T Woods
8. Kyle J Hewitt

• Curated by eLife

  Evaluation Summary:

  This is an interesting study describing the role of Samd14-capping protein (CP) complex in stress erythroid signaling. Using orthogonal cellular, biochemical and genetic complementation approaches, the authors provide evidence to establish a previously unrecognized mechanism for Samd14-CP interaction in regulating Kit signaling in erythroid regeneration in response to acute anemia. Findings of this work will be of broad interest to the study of erythropoiesis and cellular signaling.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife