Receptor-binding domain of SARS-CoV-2 is a functional αv-integrin agonist

  1. Emma G. Norris
  2. Xuan Sabrina Pan
  3. Denise C. Hocking

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Abstract

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  1. Version published to 10.1016/j.jbc.2023.102922
  2. ScreenIT

    SciScore for 10.1101/2022.04.11.487882: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Integrin-blocking antibodies anti-α5 (clone 5H10-27)
    anti-α5
    suggested: None
    For integrin blocking studies, FN-null MEFs were pre-incubated with anti-integrin antibodies (50 μg/mL) or 25 μM peptide for 1 h prior to seeding.
    anti-integrin
    suggested: None
    Bound antibodies were detected with Alexa448-, Alexa549-, or Alexa647-labeled goat anti-rabbit or -mouse secondary antibodies and visualized using a BX60 fluorescence microscope (Olympus) equipped with an epifluorescent lamp (Lumen Dynamics) and an EXi Blue Fluorescence Camera (Q Imaging), acquired with QCapture software.
    anti-rabbit
    suggested: None
    -mouse
    suggested: None
    Blots were then washed with TBS-T, incubated with horseradish peroxidase-conjugated secondary antibodies, and developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific).
    horseradish peroxidase-conjugated secondary antibodies,
    suggested: None
    Experimental Models: Organisms/Strains
    SentencesResources
    phosphotyrosine (clone 4G10, Sigma or PY20, BD Biosciences); phospho-FAK pY407 (polyclonal, Invitrogen #44650G); phospho-FAK pY397 (polyclonal, Biosource #44-624G); phospho-Src pY418 (polyclonal, Biosource #44-660); phospho-Paxillin pY118 (polyclonal, Invitrogen #44-722G); phospho-Paxillin pY31 (polyclonal, Invitrogen #44-720G); phospho-Akt pS473 (polyclonal, Cell Signaling #9271); TRITC-labeled phalloidin (Millipore, #90228)
    phospho-Paxillin pY118
    suggested: None
    Recombinant DNA
    SentencesResources
    phosphotyrosine (clone 4G10, Sigma or PY20, BD Biosciences); phospho-FAK pY407 (polyclonal, Invitrogen #44650G); phospho-FAK pY397 (polyclonal, Biosource #44-624G); phospho-Src pY418 (polyclonal, Biosource #44-660); phospho-Paxillin pY118 (polyclonal, Invitrogen #44-722G); phospho-Paxillin pY31 (polyclonal, Invitrogen #44-720G); phospho-Akt pS473 (polyclonal, Cell Signaling #9271); TRITC-labeled phalloidin (Millipore, #90228)
    pY31
    suggested: RRID:Addgene_84746)
    pS473
    suggested: None
    Software and Algorithms
    SentencesResources
    phosphotyrosine (clone 4G10, Sigma or PY20, BD Biosciences); phospho-FAK pY407 (polyclonal, Invitrogen #44650G); phospho-FAK pY397 (polyclonal, Biosource #44-624G); phospho-Src pY418 (polyclonal, Biosource #44-660); phospho-Paxillin pY118 (polyclonal, Invitrogen #44-722G); phospho-Paxillin pY31 (polyclonal, Invitrogen #44-720G); phospho-Akt pS473 (polyclonal, Cell Signaling #9271); TRITC-labeled phalloidin (Millipore, #90228)
    BD Biosciences)
    suggested: None
    All statistical analyses were performed using GraphPad Prism (version 9).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.04.11.487882v1 on bioRxiv