Cell Biology

Crotamiton derivative JM03 extends lifespan and improves oxidative and hypertonic stress resistance in Caenorhabditis elegans via inhibiting OSM-9

1. Keting Bao
2. Wenwen Liu
3. Zhouzhi Song
4. Jiali Feng
5. Zhifan Mao
6. Lingyuan Bao
7. Tianyue Sun
8. Zelan Hu
9. Jian Li

• Curated by eLife

  Evaluation Summary:

  Bao and colleagues present a chemical genetics study to identify novel compounds extending lifespan in C. elegans, and they proceed to investigate the mechanisms of action of their most potent compound. Based on the known target TRPV4 of the screening hit, the authors provide evidence for the involvement of a C. elegans homolog (osm-9) in the observed phenomenon, although this evidence is not completely conclusive. This study will be of interest to researchers working on drug discovery, repurposing and lead optimization in the context of ageing.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Tumor elimination by clustered microRNAs miR-306 and miR-79 via noncanonical activation of JNK signaling

1. Zhaowei Wang
2. Xiaoling Xia
3. Jiaqi Li
4. Tatsushi Igaki

• Curated by eLife

  Evaluation Summary:

  This paper is of interest for cancer biologists studying the role microRNAs in tumor growth. The work provides links between over-expression of microRNAs, downregulation of a ubiquitin ligase, inhibition of JNK and reduced tumor growth. Some of the data are properly controlled and analyzed. However, the key claims of the manuscript are not entirely supported by the data, and additional controls are needed.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Contour : A semi-automated segmentation and quantitation tool for cryo-soft-X-ray tomography

1. Kamal L. Nahas
2. João Ferreira Fernandes
3. Nina Vyas
4. Colin Crump
5. Stephen Graham
6. Maria Harkiolaki

Reviewed by Review Commons

Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations

1. Linda K Krasniewski
2. Papiya Chakraborty
3. Chang-Yi Cui
4. Krystyna Mazan-Mamczarz
5. Christopher Dunn
6. Yulan Piao
7. Jinshui Fan
8. Changyou Shi
9. Tonya Wallace
10. Cuong Nguyen
11. Isabelle A Rathbun
12. Rachel Munk
13. Dimitrios Tsitsipatis
14. Supriyo De
15. Payel Sen
16. Luigi Ferrucci
17. Myriam Gorospe

• Curated by eLife

  Evaluation Summary:

  Krasniewski and colleagues leveraged single-cell transcriptomics to identify subpopulations of macrophages in the skeletal muscle of aging male mice. They identify several new resident subpopulations of skeletal muscle macrophages, spanning a range of polarization states using novel markers, and they identify a shift in relative abundances of these subpopulations with age, leading to a functional shift in inflammatory marker expression and phagocytic capacity. The study overall is an interesting and timely investigation of skeletal muscle macrophage populations and transcriptomics in both healthy young and old mice, and should be a valuable resource for the inflammaging and muscle biology field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina

1. Swathy Babu
2. Yuki Takeuchi
3. Ichiro Masai

• Curated by eLife

  Evaluation Summary:

  This study reports the phenotype of a zebrafish banp mutant that was identified in a screen for eye defects. Banp is known to regulate the transcription of a number of genes including those important in oncogenesis. In vivo function of Banp, especially in the context of normal development, remains to be better understood. The current study fills this knowledge gap and identifies the roles of banp during replication stress responses and mitosis. With somewhat more careful interpretation of the data and a clearer presentation of the results and their potential impact, this study will be of interest to scientists studying development, DNA damage responses and apoptosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Phosphoregulation of DSB-1 mediates control of meiotic double-strand break activity

1. Heyun Guo
2. Ericca L Stamper
3. Aya Sato-Carlton
4. Masa A Shimazoe
5. Xuan Li
6. Liangyu Zhang
7. Lewis Stevens
8. KC Jacky Tam
9. Abby F Dernburg
10. Peter M Carlton

• Curated by eLife

  Evaluation Summary:

  The connection between double-strand break (DSB) formation and chromosome pairing/synapsis during meiosis is not fully understood. In this paper, the authors show that the formation of DSBs is regulated by the DNA damage response (DDR) machinery. The paper will be of interest to the broad meiosis and DDR communities. While the main conclusions of the manuscript appear to be well-supported by the data, some gaps are present and the manuscript would therefore benefit from additional (mostly minor) changes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Subcellular mapping of the protein landscape of SARS-CoV-2 infected cells for target-centric drug repurposing

1. Jayasankar Mohanakrishnan Kaimal
2. Marianna Tampere
3. Trang H. Le
4. Ulrika Axelsson
5. Hao Xu
6. Hanna Axelsson
7. Anna Bäckström
8. Francesco Marabita
9. Elisabeth Moussaud-Lamodière
10. Duncan Njenda
11. Carolina Oses Sepulveda
12. Wei Oyuang
13. Brinton Seashore-Ludlow
14. Caroline Vernersson
15. Ali Mirazimi
16. Emma Lundberg
17. Päivi Östling
18. Charlotte Stadler

Reviewed by ScreenIT

Discovery of a druggable copper-signaling pathway that drives cell plasticity and inflammation

1. Stéphanie Solier
2. Sebastian Müller
3. Tatiana Cañeque
4. Antoine Versini
5. Leeroy Baron
6. Pierre Gestraud
7. Nicolas Servant
8. Laila Emam
9. Arnaud Mansart
10. G. Dan Pantoș
11. Vincent Gandon
12. Valentin Sencio
13. Cyril Robil
14. François Trottein
15. Anne-Laure Bègue
16. Hélène Salmon
17. Sylvère Durand
18. Ting-Di Wu
19. Nicolas Manel
20. Alain Puisieux
21. Mark A. Dawson
22. Sarah Watson
23. Guido Kroemer
24. Djillali Annane
25. Raphaël Rodriguez

Reviewed by ScreenIT

Adaptor linked K63 di-ubiquitin activates Nedd4/Rsp5 E3 ligase

1. Lu Zhu
2. Qing Zhang
3. Ciro D Cordeiro
4. Sudeep Banjade
5. Richa Sardana
6. Yuxin Mao
7. Scott D Emr

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to researchers in the field of protein quality control, as it describes a mechanism for regulating the activity of NEDD4 E3 ubiquitin ligases. The data largely support the main conclusions of the paper.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus

1. Natasha M Puri
2. Giovanna R Romano
3. Ting-Yu Lin
4. Quynh N Mai
5. Roshanak Irannejad

• Curated by eLife

  Evaluation Summary:

  This study uses a tour de force of biosensor constructs providing evidence that dopamine transport by OCT2 across the plasma membrane and also (presumably) into the Golgi activates GPCR signaling at the Golgi leading to cAMP production and PKA activation. Thus, intracellularly compartmentalized signaling underlies aspects of Dopamine D1 receptor signaling. The work will be of interest to scientists working on the cell biology of dopamine signaling. While the data support the model overall, there are concerns that need to be addressed including specificity of the reagents used and the actual intracellular localization of D1DR and OCT2.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife