Cell Biology

Claudin5 protects the peripheral endothelial barrier in an organ and vessel-type-specific manner

1. Mark Richards
2. Emmanuel Nwadozi
3. Sagnik Pal
4. Pernilla Martinsson
5. Mika Kaakinen
6. Marleen Gloger
7. Elin Sjöberg
8. Katarzyna Koltowska
9. Christer Betsholtz
10. Lauri Eklund
11. Sofia Nordling
12. Lena Claesson-Welsh

• Curated by eLife

  Evaluation Summary:

  This manuscript provides new knowledge of one of the major molecules, claudin5, expected to maintain the integrity of the cell-cell contacts of the blood vessel wall. The results highlight organ and vessel-type specific significance of this mechanism in the regulation of vascular permeability, partially challenging the current view. A combination of in vivo microscopy and genetic mouse models is used to support the key claims in the paper. This manuscript will be of interest to scientists across vascular biology, and especially in the field of vascular permeability regulation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

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An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae

1. Annemarie Kralt
2. Matthias Wojtynek
3. Jonas S Fischer
4. Arantxa Agote-Aran
5. Roberta Mancini
6. Elisa Dultz
7. Elad Noor
8. Federico Uliana
9. Marianna Tatarek-Nossol
10. Wolfram Antonin
11. Evgeny Onischenko
12. Ohad Medalia
13. Karsten Weis

• Curated by eLife

  Evaluation Summary:

  This work advances our understanding of the nuclear pore complex (NPC) biogenesis pathway by providing much needed additional insight into the function of one of the few NPC assembly factors, Brl1. It thus addresses a long-standing and fundamental question relevant to individuals interested in nuclear transport, nuclear cell biology, and membrane-protein interactions more generally.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

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Subcellular Dynamic Immunopatterning of Cytosolic Protein Complexes on Microstructured Polymer Substrates

1. Roland Hager
2. Ulrike Müller
3. Nicole Ollinger
4. Julian Weghuber
5. Peter Lanzerstorfer

• Curated by eLife

  Evaluation Summary:

  This manuscript describes a method of evaluating the steady state levels and kinetics of protein-protein interactions at the plasma membrane of living cells. This approach builds on previous work in this area, and with stronger validation and demonstration of biological applications, it may be complementary to other biochemical or imaging-based approaches to address important questions related to mechanisms of cell signaling.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

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A BORC-dependent molecular pathway for vesiculation of cell corpse phagolysosomes

1. Gholamreza Fazeli
2. Roni Levin-Konigsberg
3. Michael C. Bassik
4. Christian Stigloher
5. Ann M. Wehman

Reviewed by Review Commons

Genome editing in the unicellular holozoan Capsaspora owczarzaki suggests a premetazoan role for the Hippo pathway in multicellular morphogenesis

1. Jonathan E Phillips
2. Maribel Santos
3. Mohammed Konchwala
4. Chao Xing
5. Duojia Pan

• Curated by eLife

  Evaluation Summary:

  In this study, Phillips et al. report the ancient role of Hippo signaling nuclear effector YAP/TAZ/Yorkie ortholog (coYki) in a unicellular organism Capsaspora owczarzaki. Two major advances of this work include development of genome editing in Capsaspora owczarzaki and characterization of a key gene coYki. The authors found that different from Yki's role in multicellular organisms, the coYki does not contribute to cell proliferation. Instead, it affects cell adhesion to extracellular matrix to ensure the spherical shape of the aggregates. Overall, the presentation of this manuscript is clear with a nice logical flow, and this study should be of interest to the evolutionary cell biology field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

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Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex

1. Meng Shi
2. Hieng Chiong Tie
3. Mahajan Divyanshu
4. Xiuping Sun
5. Yan Zhou
6. Boon Kim Boh
7. Leah A Vardy
8. Lei Lu

• Curated by eLife

  Evaluation Summary:

  The mechanisms of TGF-beta signaling have been intensively investigated. In this study, the authors reveal a novel mechanism of TGF-beta regulation, which suggests a higher order of signaling complexity. With some stronger experimental support, the paper will be of interest to those studying signal transduction.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

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The KASH5 protein involved in meiotic chromosomal movements is a novel dynein activating adaptor

1. Ritvija Agrawal
2. John P Gillies
3. Juliana L Zang
4. Jingjing Zhang
5. Sharon R Garrott
6. Hiroki Shibuya
7. Jayakrishnan Nandakumar
8. Morgan E DeSantis

• Curated by eLife

  Evaluation Summary:

  This study contributes to our understanding of how a diverse and increasing number of activating adaptors allow the dynein motor protein to move a wide range of intracellular cargoes. Here the authors identify a transmembrane protein called KASH5 as the activating adaptor required for dynein to move meiotic chromosomes, a process that facilitates homolog pairing. Overall, the work is well done and will be of interest to the cell biology, cytoskeletal, and meiosis research communities.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

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Three-dimensional flagella structures from animals’ closest unicellular relatives, the Choanoflagellates

1. Justine M Pinskey
2. Adhya Lagisetty
3. Long Gui
4. Nhan Phan
5. Evan Reetz
6. Amirrasoul Tavakoli
7. Gang Fu
8. Daniela Nicastro

• Curated by eLife

  Evaluation Summary:

  Cryo-electron tomography has been successful at uncovering the details of the eukaryotic cilium/flagellum but there remains a limited number of taxa represented in the studies to date. Pinskey et al. fill this gap with a flagellar structure from choanoflagellates: single-celled organisms that are the closest living relatives of animals. The findings yield many new insights of broad interest to the field, such as the similarity of outer dynein arms and radial spokes to metazoan cilia, the observation of a flagellar vanes, and the presence of mysterious barb structures.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

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A nucleation barrier spring-loads the CBM signalosome for binary activation

1. Alejandro Rodriguez Gama
2. Tayla Miller
3. Jeffrey J Lange
4. Jay R Unruh
5. Randal Halfmann

Reviewed by Review Commons

C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9

1. Saskia-Larissa Jauch-Speer
2. Marisol Herrera-Rivero
3. Nadine Ludwig
4. Bruna Caroline Véras De Carvalho
5. Leonie Martens
6. Jonas Wolf
7. Achmet Imam Chasan
8. Anika Witten
9. Birgit Markus
10. Bernhard Schieffer
11. Thomas Vogl
12. Jan Rossaint
13. Monika Stoll
14. Johannes Roth
15. Olesja Fehler

• Curated by eLife

  Evaluation Summary:

  The manuscript by Jauch-Speer and colleagues uses a CRISPR/Cas9 screening approach in a myeloid cell line to identify C/EBP-delta as a regulator of the alarmins S100A8 and S100A9, which amplify inflammation. This paper is of great interest to macrophage biologists studying macrophage function in inflammatory diseases. In an elegant series of gene targeting and sequencing studies, the authors also characterized epigenetic mechanisms regulating the expression of these pro-inflammatory mediators. Furthermore, human monocytes from cardiovascular patient cohorts showed correlative changes, indicating possible clinical relevance.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

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