Cell Biology

The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers

1. Martina Oravcová
2. Minghua Nie
3. Nicola Zilio
4. Shintaro Maeda
5. Yasaman Jami-Alahmadi
6. Eros Lazzerini-Denchi
7. James A Wohlschlegel
8. Helle D Ulrich
9. Takanori Otomo
10. Michael N Boddy

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to the chromosome biology field and SMC researchers in particular. The study provides cell biological, biochemical, and structural modeling evidence that a new Nse5-like protein named SIMC1 is a paralog of SLF1, and that the two compete for SLF2-Smc5/6 binding. The authors also show that SIMC1 targets SMC5/6 to polyomavirus replication centers through its SUMO binding motifs (SIMs), supporting a role for SIMC1 in Smc5/6 recruitment for viral restriction.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Deconstructing cold-induced brown adipocyte neogenesis in mice

1. Rayanne B Burl
2. Elizabeth Ann Rondini
3. Hongguang Wei
4. Roger Pique-Regi
5. James G Granneman

• Curated by eLife

  Evaluation Summary:

  Burl and Rondini et al. elucidate the transcriptional profile of the stromal vascular fraction of murine brown adipose tissue in the context of thermogenic stimulation. The authors combined systems and reductionist approaches to show the reliance of mature brown adipocytes on adrenergic activation to indirectly stimulate progenitor proliferation and differentiation and the involvement of dendritic cells in this process. Overall, this is a timely and well-rounded work that will provide beneficial data for public use and further resolve the complexities underlying brown adipose physiology.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Manganese is a physiologically relevant TORC1 activator in yeast and mammals

1. Raffaele Nicastro
2. Hélène Gaillard
3. Laura Zarzuela
4. Marie-Pierre Péli-Gulli
5. Elisabet Fernández-García
6. Mercedes Tomé
7. Néstor García-Rodríguez
8. Raúl V Durán
9. Claudio De Virgilio
10. Ralf Erik Wellinger

• Curated by eLife

  Evaluation Summary:

  Nicastro et al. uncover a new input for the central metabolic regulator TOR complex 1 (TORC1) , namely manganese (Mn) levels, in budding yeast and they show that this dependence on Mn is conserved in humans. TORC1 is a central coordinator of multiple inputs to guide cellular decisions of catabolism vs anabolism, and information on an additional way to modulate its activity will be highly influential in both basic cell biology as well as therapeutic research.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1

Reviewed by eLife

Independent regulation of mitochondrial DNA quantity and quality in Caenorhabditis elegans primordial germ cells

1. Aaron ZA Schwartz
2. Nikita Tsyba
3. Yusuff Abdu
4. Maulik R Patel
5. Jeremy Nance

• Curated by eLife

  Evaluation Summary:

  Mitochondria have their own DNA, which is much more likely to gain mutation (due to error-prone DNA polymerase). It is widely appreciated that there are quality control mechanisms such that functional mitochondria are passed from one generation to the next. The proposed mechanisms include a passive mechanism (generation of the bottleneck) as well as an active mechanism (selective removal of non-functional mitochondria), but the processes are not fully understood. This manuscript presents fascinating observations as to how C. elegans germline may remove mitochondria by creating bottlenecks as well as selectively removing non-functional mitochondria. Building upon the authors' previous finding that the C. elegans primordial germ cells (PGCs) shed much of cytoplasm during embryogenesis through 'cannibalism', they now describe that a bulk of mitochondria are removed from PGCs through this process.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

HIF-1α induces glycolytic reprograming in tissue-resident alveolar macrophages to promote cell survival during acute lung injury

1. Parker S Woods
2. Lucas M Kimmig
3. Kaitlyn A Sun
4. Angelo Y Meliton
5. Obada R Shamaa
6. Yufeng Tian
7. Rengül Cetin-Atalay
8. Willard W Sharp
9. Robert B Hamanaka
10. Gökhan M Mutlu

• Curated by eLife

  Evaluation Summary:

  This study provides important new information regarding the functions and behavior of lung tissue-resident alveolar macrophages in the context of acute lung injury. New data regarding the impact of hypoxia via HIF-1 on biology of these critical cells are provided and importantly suggesting an impact on the outcome of lung injury.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis

1. Kelvin W Pond
2. Julia M Morris
3. Olga Alkhimenok
4. Reeba P Varghese
5. Carly R Cabel
6. Nathan A Ellis
7. Jayati Chakrabarti
8. Yana Zavros
9. Juanita L Merchant
10. Curtis A Thorne
11. Andrew L Paek

• Curated by eLife

  Evaluation Summary:

  Pond et al. established a 2D patient-derived organoid screening platform to study tissue patterning and kinase pathway dynamics. They aim to understand how the spacing of different colonic cell types and their communication are regulated. They found that apoptosis induces Erk signaling waves that prime cell movement and are essential to maintain tissue patterning in the organoid monolayers. The work presented here is of importance to the field and provides insights into how Erk waves driven by apoptosis can help maintain gut homeostasis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

DetecDiv, a generalist deep-learning platform for automated cell division tracking and survival analysis

1. Théo Aspert
2. Didier Hentsch
3. Gilles Charvin

• Curated by eLife

  Evaluation Summary:

  This article introduces "DetecDiv", a high throughput, deep learning method to perform automated cell-division tracking in yeast. The performance of the method, estimated to be 100 times faster than manual annotation, overcomes current time processing limitations that are inherent to large single cell datasets. In particular, DetecDiv allows to automatically get quantitative measurements of replicative life span in yeast. The method is of broad interest for quantitative biology as it can be used to study yeast cells dynamics across their lifespan.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transport

1. Katharina MC Klee
2. Michael W Hess
3. Michael Lohmüller
4. Sebastian Herzog
5. Kristian Pfaller
6. Thomas Müller
7. Georg F Vogel
8. Lukas A Huber

• Curated by eLife

  Evaluation Summary:

  Here, the authors performed a CRISPR/Cas9 loss of function screen in polarized human epithelial cells to identify novel regulators of epithelial polarization and polarized membrane trafficking. This study provides a powerful resource for future investigations to unravel the complexity and diversity of mechanisms underlying epithelial polarization and polarized cargo transport. Furthermore, this dataset may represent an essential contribution to investigating novel congenital diseases associated with these processes, e.g., microvillus inclusion disease and necrotizing enterocolitis. A few experiments are suggested to bolster the authors' conclusions regarding the roles of key hits.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Molecular architecture of the C. elegans centriole

1. Alexander Woglar
2. Marie Pierron
3. Fabian Zacharias Schneider
4. Keshav Jha
5. Coralie Busso
6. Pierre Gönczy

Reviewed by Review Commons

Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase

1. Ana Pimenta-Marques
2. Tania Perestrelo
3. Patricia Rodrigues
4. Paulo Duarte
5. Mariana Lince-Faria
6. Mónica Bettencourt-Dias

Reviewed by Review Commons