Cell Biology

Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa

1. Anita Szőke
2. Orsolya Sárkány
3. Géza Schermann
4. Orsolya Kapuy
5. Axel CR Diernfellner
6. Michael Brunner
7. Norbert Gyöngyösi
8. Krisztina Káldi

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest to researchers working in chronobiology and metabolism. The authors have found evidence that starvation decreases the abundance of the fungal circadian clock protein white collar complex (WCC), even though WC-1 is required for responses to starvation. This observation is interesting, but the authors should consider that WCC has several other functions (as a light receptor, in transcriptional regulation) that are not necessarily clock connected. As such the most interesting result from this paper is that the standard model for the molecular mechanism of the fungal circadian clock does not explain the persistence of normal rhythms under extreme starvation conditions, where the levels of clock proteins are drastically altered.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

β-cell deletion of the PKm1 and PKm2 isoforms of pyruvate kinase in mice reveals their essential role as nutrient sensors for the KATP channel

1. Hannah R Foster
2. Thuong Ho
3. Evgeniy Potapenko
4. Sophia M Sdao
5. Shih Ming Huang
6. Sophie L Lewandowski
7. Halena R VanDeusen
8. Shawn M Davidson
9. Rebecca L Cardone
10. Marc Prentki
11. Richard G Kibbey
12. Matthew J Merrins

• Curated by eLife

  **Evaluation Summary:
  **
  This manuscript employs in vitro studies and elegant mouse models to detail how specific pyruvate kinase isoforms impact pancreatic beta-cell ATP/ADP levels, ATP-sensitive K+ channel (KATP channel) activity, calcium handling, and insulin secretion. This is an important study that challenges the current paradigms of KATP-channel regulation, the major signaling mechanism through which pancreatic beta cells couple blood glucose levels to insulin release. Future studies will be necessary to determine whether similar mechanisms are used in human pancreatic beta cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Interoperability of RTN1A in dendrite dynamics and immune functions in human Langerhans cells

1. Małgorzata Anna Cichoń
2. Karin Pfisterer
3. Judith Leitner
4. Lena Wagner
5. Clement Staud
6. Peter Steinberger
7. Adelheid Elbe-Bürger

• Curated by eLife

  Evaluation Summary:

  This is an excellent manuscript that addresses the role of the molecule (RETICULON 1A / RTN1A) in the biology of human Langerhans cells (the epidermal resident dendritic cell). The study shows that RTN1A critically regulates the retention within the epidermis versus the emigration from the epidermis of these cells. Since Langerhans cells are central in the induction of immune responses (e.g. in vaccinations, allergic hypersensitivities) as well as in the maintenance of immunological tolerance (e.g. in autoimmune diseases of the skin) this manuscript will be of wide interest to the scientific community working in the fields of immunology/vaccinology, dermatology, cell biology and beyond.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Age-dependent aggregation of ribosomal RNA-binding proteins links deterioration in chromatin stability with challenges to proteostasis

1. Julie Paxman
2. Zhen Zhou
3. Richard O'Laughlin
4. Yuting Liu
5. Yang Li
6. Wanying Tian
7. Hetian Su
8. Yanfei Jiang
9. Shayna E Holness
10. Elizabeth Stasiowski
11. Lev S Tsimring
12. Lorraine Pillus
13. Jeff Hasty
14. Nan Hao

• Curated by eLife

  Evaluation Summary:

  The idea of individual aging trajectories of single cells is important and the authors provide sufficient evidence that there is some stochasticity that directs individual cells towards certain routes of aging - at least in budding yeast. Investigating the link between rDNA silencing and protein homeostasis, this study thus addresses an interesting and exciting question. The authors show how age-dependent loss of rDNA silencing might contribute to protein aggregation. Importantly, the paper furthers the understanding of distinct aging trajectories and raises important questions about how these processes might be relevant in multicellular organisms.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Replicative aging impedes stress-induced assembly of a key human protein disaggregase

1. Yasith Mathangasinghe
2. Niels Alberts
3. Carlos J. Rosado
4. Dezerae Cox
5. Natalie L. Payne
6. Angelique R. Ormsby
7. Keziban Merve Alp
8. Roman Sakson
9. Sanjeev Uthishtran
10. Thomas Ruppert
11. Senthil Arumugam
12. Danny M. Hatters
13. Harm H. Kampinga
14. Nadinath B. Nillegoda

Reviewed by ASAPbio crowd review

Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for label-free detection of molecules across entire cell sections

1. Johannes Elferich
2. Giulia Schiroli
3. David T Scadden
4. Nikolaus Grigorieff

• Curated by eLife

  Evaluation Summary:

  The work details a new acquisition method of defocus corrected large area cryo-EM (DeCo-LACE). The data-acquisition approach is highly complementary to the research group's previous work of using high-resolution 2D template-matching (2DTM) to identify macromolecular complexes in dense and heterogeneous cellular specimens. Notably and importantly, the data-acquisition approach minimizes sampling bias. Overall, DeCo-LACE is a very interesting approach to locating large ribosomal subunits in FIB-lamella at scale.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Phosphoproteomic mapping reveals distinct signaling actions and activation of muscle protein synthesis by Isthmin-1

1. Meng Zhao
2. Niels Banhos Danneskiold-Samsøe
3. Livia Ulicna
4. Quennie Nguyen
5. Laetitia Voilquin
6. David E Lee
7. James P White
8. Zewen Jiang
9. Nickeisha Cuthbert
10. Shrika Paramasivam
11. Ewa Bielczyk-Maczynska
12. Capucine Van Rechem
13. Katrin J Svensson

• Curated by eLife

  Evaluation Summary:

  This manuscript presents evidence that the adipocyte-derived protein Ism1, which signals through a typical receptor tyrosine kinase, induces unique phosphoproteome signatures when compared to insulin, and regulates skeletal muscle force production. The manuscript should be of interest to those who study integrated physiology and skeletal muscle physiology. While the data suggest there may be some effects on myofiber size, further study is needed before any conclusions can be made as to what, if any, effects Ism1 has on myofiber size.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Inflammatory stress signaling via NF-kB alters accessible cholesterol to upregulate SREBP2 transcriptional activity in endothelial cells

1. Joseph Wayne M Fowler
2. Rong Zhang
3. Bo Tao
4. Nabil E Boutagy
5. William C Sessa

• Curated by eLife

  Evaluation Summary:

  This study shows a direct link between inflammation and cholesterol metabolism in endothelial cells. Specifically, the authors show a pathway by which the major inflammatory factor, NF kappa B, activates a gene called STARD10, which, in turn, leads to the activation of the cholesterol biosynthetic pathway. The study, therefore, provides important insights into the inter-relationship between cholesterol metabolism and inflammation at the molecular level.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers

1. Valentina Štimac
2. Isabella Koprivec
3. Martina Manenica
4. Juraj Simunić
5. Iva M Tolić

Reviewed by Review Commons

Confined migration induces heterochromatin formation and alters chromatin accessibility

1. Chieh-Ren Hsia
2. Jawuanna McAllister
3. Ovais Hasan
4. Julius Judd
5. Seoyeon Lee
6. Richa Agrawal
7. Chao-Yuan Chang
8. Paul Soloway
9. Jan Lammerding

Reviewed by ASAPbio crowd review