Cell Biology

The small GTPase ARF3 controls invasion modality and metastasis by regulating N-cadherin levels

1. Emma Sandilands
2. Eva C. Freckmann
3. Erin M. Cumming
4. Alvaro Román-Fernández
5. Lynn McGarry
6. Jayanthi Anand
7. Laura Galbraith
8. Susan Mason
9. Rachana Patel
10. Colin Nixon
11. Jared Cartwright
12. Hing Y. Leung
13. Karen Blyth
14. David M. Bryant

Reviewed by Review Commons

A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins

1. Miwa Umebayashi
2. Satoko Takemoto
3. Luc Reymond
4. Mayya Sundukova
5. Ruud Hovius
6. Annalisa Bucci
7. Paul A. Heppenstall
8. Hideo Yokota
9. Kai Johnsson
10. Howard Riezman

Reviewed by Review Commons

Matrix stiffness regulates Notch signaling activity in endothelial cells

1. Maibritt Kretschmer
2. Rose Mamistvalov
3. David Sprinzak
4. Angelika M. Vollmar
5. Stefan Zahler

Reviewed by Review Commons

DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice

1. Yi Tian Yap
2. Wei Li
3. Qian Huang
4. Qi Zhou
5. David Zhang
6. Yi Sheng
7. Ljljiana Mladenovic-Lucas
8. Siu-Pok Yee
9. Kyle E Orwig
10. James G Granneman
11. David C Williams
12. Rex A Hess
13. Aminata Toure
14. Zhibing Zhang

• Curated by eLife

  Evaluation Summary:

  This paper is of potential interest to a broad audience in the fields of germ cell biology and cytoskeleton, as it implies a microtubule-based motor function in intra-manchette cargo transport in developing sperm tail. However, some conclusions of this paper require stronger experimental support.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits

1. Ziru Li
2. Emily Bowers
3. Junxiong Zhu
4. Hui Yu
5. Julie Hardij
6. Devika P Bagchi
7. Hiroyuki Mori
8. Kenneth T Lewis
9. Katrina Granger
10. Rebecca L Schill
11. Steven M Romanelli
12. Simin Abrishami
13. Kurt D Hankenson
14. Kanakadurga Singer
15. Clifford J Rosen
16. Ormond A MacDougald

• Curated by eLife

  Evaluation Summary:

  This is an exemplary manuscript describing the creation of a novel mouse model to study bone marrow adipocytes. The authors demonstrate that these cells play a critical role in the pathophysiology of myeloid cell lineage regeneration as well as the maintenance of bone mass and hematopoietic progenitors during times of limited energy (e.g. caloric restriction).

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Clathrin coats partially preassemble and subsequently bend during endocytosis

1. Markus Mund
2. Aline Tschanz
3. Yu-Le Wu
4. Felix Frey
5. Johanna L. Mehl
6. Marko Kaksonen
7. Ori Avinoam
8. Ulrich S. Schwarz
9. Jonas Ries

Reviewed by Review Commons

The integrated stress response remodels the microtubule-organizing center to clear unfolded proteins following proteotoxic stress

1. Brian Hurwitz
2. Nicola Guzzi
3. Anita Gola
4. Vincent F Fiore
5. Ataman Sendoel
6. Maria Nikolova
7. Douglas Barrows
8. Thomas S Carroll
9. H Amalia Pasolli
10. Elaine Fuchs

• Curated by eLife

  Evaluation Summary:

  This interesting study identifies why or how the integrated stress response pathway regulates cell recovery upon proteotoxic stress, which is especially interesting in cancer cells resistant to proteasome inhibitors. The authors conclude that translation initiation of mRNAs encoding microtubule cytoskeleton, centrosome and ATF5 proteins is necessary to recover from proteotoxic stress. This paper will make a strong contribution to the literature.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Prolonged β-adrenergic stimulation disperses ryanodine receptor clusters in cardiomyocytes and has implications for heart failure

1. Xin Shen
2. Jonas van den Brink
3. Anna Bergan-Dahl
4. Terje R Kolstad
5. Einar S Norden
6. Yufeng Hou
7. Martin Laasmaa
8. Yuriana Aguilar-Sanchez
9. Ann P Quick
10. Emil KS Espe
11. Ivar Sjaastad
12. Xander HT Wehrens
13. Andrew G Edwards
14. Christian Soeller
15. William E Louch

• Curated by eLife

  Evaluation Summary:

  This study applies super-resolution imaging to the distribution of Ca2+ release channels before and after adrenergic stimulation. They make comparisons between healthy and failing cardiomyocytes. The results are specifically applicable to the understanding of contractile function in cardiac failure.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Lithium-induced ciliary lengthening sparks Arp2/3 complex–dependent endocytosis

1. Brae M. Bigge
2. Larissa L. Dougherty
3. Prachee Avasthi

Reviewed by Review Commons

A unified view of low complexity regions (LCRs) across species

1. Byron Lee
2. Nima Jaberi-Lashkari
3. Eliezer Calo

• Curated by eLife

  Evaluation Summary:

  This paper describes a new dotplot-based approach for analyzing Low Complexity Regions (LCRs) in proteins. The work is validated against a single protein, compared to existing methods, and applied to the proteomes of several model systems. The work aims to show links between specific LCRs and biological function and subcellular location, and study conservation in LCRs amongst higher species.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife