Cell Biology

Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families

1. Beverley Wilson
2. Chloe Flett
3. Jakub Gemperle
4. Craig Lawless
5. Matthew Hartshorn
6. Eleanor Hinde
7. Tess Harrison
8. Megan Chastney
9. Sarah Taylor
10. Jennifer Allen
11. Jim C. Norman
12. Thomas Zacharchenko
13. Patrick T. Caswell

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Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation

1. Jonay Garcia-Luis
2. Hélène Bordelet
3. Agnès Thierry
4. Romain Koszul
5. Luis Aragon

• Curated by eLife

  Evaluation Summary:

  Cohesin is an evolutionarily conserved protein complex that plays essential roles in mitotic chromosome structure and function. Previous studies suggest that multiple activities of cohesin are required only prior to the onset of chromosome segregation. Using a Mcd1-AID and a Mcd1-TEV to either degrade or cleave cohesin's kleisin subunit Mcd1 of the yeast Saccharomyces cerevisiae, this study shows that cohesion plays also a role in anaphase organizing the centromeric regions, providing new evidence that cohesin function is critical for chromosome structure and segregation during and after the onset of chromosome segregation. The work is of relevance for students of chromosome biology and cell division.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Transcriptional regulation of Sis1 promotes fitness but not feedback in the heat shock response

1. Rania Garde
2. Abhyudai Singh
3. Asif Ali
4. David Pincus

• Curated by eLife

  Evaluation Summary:

  In this paper the authors report an updated theoretical model describing in mathematical terms how the Hsf1 transcription factor is activated in yeast in response to heat shock, and demonstrate that rather than denatured mature proteins, Hsf1 activation involves newly synthesized proteins that sequester the Hsp70 chaperone away from the inactive Hsp70/Hsf1 complex, releasing active Hsf1. They also describe a general role for the Sis1 co-chaperone in maintaining the fitness of yeast cells under stress conditions, such as heat shock, that is independent of regulation of Hsf1.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

PtdIns(3,4)P2, Lamellipodin, and VASP coordinate actin dynamics during phagocytosis in macrophages

1. Fernando Montaño-Rendón
2. Glenn F.W. Walpole
3. Matthias Krause
4. Gerald R.V. Hammond
5. Sergio Grinstein
6. Gregory D. Fairn

Reviewed by Review Commons

Proteogenomic analysis of cancer aneuploidy and normal tissues reveals divergent modes of gene regulation across cellular pathways

1. Pan Cheng
2. Xin Zhao
3. Lizabeth Katsnelson
4. Elaine M Camacho-Hernandez
5. Angela Mermerian
6. Joseph C Mays
7. Scott M Lippman
8. Reyna Edith Rosales-Alvarez
9. Raquel Moya
10. Jasmine Shwetar
11. Dominic Grun
12. David Fenyo
13. Teresa Davoli

• Curated by eLife

  Evaluation Summary:

  This work by Cheng et al evaluates the contribution of regulation of gene expression at the RNA and protein level by leveraging copy number variations in a large cohort of cancer samples. Importantly they find that there is rarely compensatory regulation at the RNA and protein level together, but depending on the gene, expression is either compensated at one or the other. The paper is very intriguing and the findings are of interest to a broad readership.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Selective endocytosis controls slit diaphragm maintenance and dynamics in Drosophila nephrocytes

1. Konrad Lang
2. Julian Milosavljevic
3. Helena Heinkele
4. Mengmeng Chen
5. Lea Gerstner
6. Dominik Spitz
7. Severine Kayser
8. Martin Helmstädter
9. Gerd Walz
10. Michael Köttgen
11. Andrew Spracklen
12. John Poulton
13. Tobias Hermle

• Curated by eLife

  Evaluation Summary:

  This paper would be of interest to all researchers who work in understanding the mechanisms involved in podocyte slit diaphragm homeostasis and maintenance of the glomerular filtration barrier. The work provides substantial new insights into nephrin dynamics and the mechanisms of slit diaphragm maintenance. A series of compelling experiments depicted that dynamin-mediated endocytosis was involved in ectopic nephrin turnover and that flotillin-mediated turnover of nephrin occurred within the slit diaphragm was needed to maintain filter permeability in-vivo.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Box C/D small nucleolar ribonucleoproteins regulate mitochondrial surveillance and innate immunity

1. Elissa Tjahjono
2. Alexey V. Revtovich
3. Natalia V. Kirienko

• Curated by eLife

  Evaluation Summary:

  This manuscript addresses a potential role for the box C/D Small Nucleolar Ribonucleoprotein complex at the intersection of mitochondrial homeostasis and cellular responses to infection and environmental stress in the context of the C. elegans system. The presented data can be explained in multiple ways and can serve as starting point for further research in this interesting and important area.

  “(This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

TRF2-mediated ORC recruitment underlies telomere stability upon DNA replication stress

1. Mitsunori Higa
2. Yukihiro Matsuda
3. Jumpei Fujii
4. Nozomi Sugimoto
5. Kazumasa Yoshida
6. Masatoshi Fujita

• Curated by eLife

  Evaluation Summary:

  This paper is of interest to biochemists studying DNA replication and genome maintenance in eukaryotic cells. The work details a structure-function analysis of an interaction between two proteins that are critical for genome stability. A mutation that disrupts this interaction may have no adverse effects under unperturbed conditions but causes telomeric DNA damage when cells experience replication stress. However, the structural nature of the damage and cellular consequences are not sufficiently explored.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing

1. Eran Yanowski
2. Nancy S. Yacovzada
3. Eyal David
4. Amir Giladi
5. Diego Jaitin
6. Lydia Farack
7. Adi Egozi
8. Danny Ben-Zvi
9. Shalev Itzkovitz
10. Ido Amit
11. Eran Hornstein

• Curated by eLife

  Evaluation Summary:

  This paper provides an attempt to understand the crosstalk between to islet cell types during beta cell regeneration following partial pancreatectomy. It combines lineage tracing, single cell sequencing and light microscopy to describe islet cell heterogeneity and interactions in the regenerating mouse pancreas. The concept of protective signaling resulting from the direct interactions between beta and delta cells is compelling and would be of interest to scientists in the field of endocrine pancreas development and regeneration. However, the conclusions derived from the sequencing data require additional experimental support.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis

1. Alexander J. Stemm-Wolf
2. Eileen T. O’Toole
3. Ryan M. Sheridan
4. Jacob T. Morgan
5. Chad G. Pearson

• Curated by eLife

  Evaluation Summary:

  This study investigates how deficiency in the RNA splicing factor SON impairs centriole assembly, which may underlie ciliopathy-like phenotypes in humans with SON mutations and is thus of interest to both cell biologists and clinicians. Using RNA-sequencing analysis and advanced imaging techniques the authors discover a large number of known and new SON splicing targets and attempt to identify those crucial for SON knockdown defects. However, knockdown of a subset of targets did not fully recapitulate SON depletion phenotypes and only led to the relatively vague conclusion that the observed centriole assembly defects were caused by impaired protein trafficking around the centrosome.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife