Cell Biology

Differential spatial regulation and activation of integrin nanoclusters inside focal adhesions

1. Sarah Keary
2. Nicolas Mateos
3. Felix Campelo
4. Maria F Garcia-Parajo

• Curated by eLife

  eLife Assessment

  In this manuscript, the authors analyse the nanoscale localisation of α5β1 and αVβ3 integrins in integrin adhesion complexes (IAC) by dual-colour STORM and DNA-Paint and assess the spatial organisation at the nano and mesoscale of their main adaptors (paxillin, talin and vinculin). This is an important work that provides detailed analyses that reveal how elements of these complex structures are really organised at the nanoscale, an essential perspective for a better understanding of how IACs function and regulate mechanotransduction processes. The evidence presented is convincing, using complementary super-resolution imaging techniques and subsequent computational modelling that enabled a quantitative assessment of the resulting data.

Reviewed by eLife

BICD2 is a centriolar protein that controls mother-daughter centriole engagement and the licensing of duplication

1. Lídia Montes-Ruiz
2. Núria Gallisà-Suñé
3. Laura Regué
4. Lorena Fernández-de-Larrea
5. Joan Roig

Reviewed by Review Commons