Cell Biology

PXGS: a Poly-Transgene Expression System based on Mutually Exclusive Splicing of Dscam

1. Renee Yin Yu
2. Alyeri Bucio-Mendez
3. Brian E Chen

• Curated by eLife

  eLife Assessment

  This valuable study describes PXGS, a poly-transgene expression system that exploits the mutually exclusive splicing of Dscam variable exon 4 to enable conditional, simultaneous expression of up to 12 transgenes in Drosophila, addressing a longstanding limitation in which conditional co-expression has been restricted to a handful of genes. The approach is conceptually elegant and technically accessible, with potential applications spanning neuroscience, synthetic biology, and biomanufacturing across arthropod species. The evidence that Dscam exon 4 splicing is preserved in a UAS vector and that individual alternates can be replaced with functional transgenes is solid, and the in vivo axonal re-wiring application provides a convincing proof of principle. Quantitative characterization of expression levels, a direct demonstration of expression across all twelve positions, and additional imaging controls would further substantiate the system's utility and scope.

Reviewed by eLife

Localized disruption of the presynaptic endoplasmic reticulum in Atlastin mutants

1. Mónica C. Quiñones-Frías
2. Dina M. Ocken
3. Avital A. Rodal

Reviewed by Review Commons

MR1 dependent MAIT cell activation is regulated by autophagy associated proteins

1. Prabhjeet Phalora
2. James Ussher
3. Svenja Hester
4. Emanuele Marchi
5. Jeffrey Y. W. Mak
6. David P. Fairlie
7. Paul Klenerman

Reviewed by Review Commons

The cell cycle variant in multiciliated cells incorporates 2 centriole biogenesis cycles

1. Amélie-Rose Boudjema
2. Rémi Balagué
3. Cayla E Jewett
4. Jacques Serizay
5. Gina M LoMastro
6. Olivier Mercey
7. Adel Al Jord
8. Adrien Candat
9. Marion Faucourt
10. Alexandre Schaeffer
11. Camille Noûs
12. Nathalie Delgehyr
13. Andrew J Holland
14. Nathalie Spassky
15. Alice Meunier

• Curated by eLife

  eLife Assessment

  In this important study, Boudjema et al. use cell culture models and high quality advanced microscopic imaging to provide detailed analyses of the cellular processes underlying centriole amplification, apical migration, and assembly of hundreds of motile cilia in multi-ciliated cells. The authors present convincing evidence showing that in these cells all the molecular and cellular steps controlling centriole biogenesis that in cycling cells extend over almost two cell cycles, occur within a single cell cycle variant. This work provides a better understanding of the regulation and order of these processes and is of interest to all cell biologists and in particular researchers studying centrioles and cilia.

Reviewed by eLife

Cell cycle dependent variation in endocytosis drives phenotypic diversity in M. tuberculosis

1. Neeraja Subhash
2. Sandhya Krishnan Radhakrishnan
3. Hitakshi Vijay
4. Neilay Bhalerao
5. Sahanawaz Molla
6. Anton Iyer
7. Shaon Chakrabarti
8. Varadharajan Sundaramurthy

• Curated by eLife

  eLife Assessment

  This useful study explores how macrophage cell-cycle state may influence endocytosis, Mycobacterium tuberculosis uptake, and the intracellular stress experienced by bacteria. While the question is interesting and the experimental approach has promise, the evidence for the central claim that endocytic capacity is specifically regulated by cell-cycle stage is incomplete. The main concern is that fluorescence-based sorting and total-fluorescence measurements likely covary with cell size, so the reported phenotypes could reflect biomass accumulation or other cell-cycle-associated changes rather than endocytic capacity as the causal determinant. As a result, whilst the study raises a hypothesis that is of importance, additional controls are required before the proposed mechanism can be considered well supported.

Reviewed by eLife

Cytosolic Carboxypeptidase 5 maintains mammalian ependymal multicilia to ensure proper homeostasis and functions of the brain

1. Rubina Dad
2. Yujuan Wang
3. Chuyu Fang
4. Yuncan Chen
5. Yi Zheng
6. Yuan Zhang
7. Xinwen Pan
8. Xinyue Zhang
9. Emily Swanekamp
10. Krish Patel
11. Matthias TF Wolf
12. Zhiguang Yuchi
13. Xueliang Zhu
14. Hui-Yuan Wu

• Curated by eLife

  eLife Assessment

  The authors developed a new Agbl5 KO allele, extending the deletion to the N-terminus of CCP5 to explore its function in mouse ependymal cells and trachea. They show that the KO mice exhibit severe hydrocephalus due to mislocated basal bodies and impaired ciliary beating. The findings are valuable with implications in the subfield of cell biology. The evidence is solid in that the methods, data and analyses largely support the claims with only a few remaining weaknesses.

Reviewed by eLife

Apical actin filament turnover mediated by cyclase-associated protein is required for organization of non-centrosomal microtubules in epithelium

1. Arathi Preeth Babu
2. Sachin Muralidharan
3. Konstantin Kogan
4. Tommi Kotila
5. Ville Hietakangas
6. Jaakko Mattila
7. Minna Poukkula

Reviewed by Review Commons

CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

1. Maria Giovanna De Leo
2. Andreas Mayer

• Curated by eLife

  eLife Assessment

  The authors present important evidence for a WIPI2-Retriever complex (termed CROP2) that couples cargo selection to carrier fission at endosomes. CROP2 appears to function analogously to the previously described CROP1 complex, formed by WIPI1 and Retromer, with which it shares structural similarities. They provide compelling evidence that CROP1 and CROP2 regulate the trafficking of distinct subsets of cargoes; however, the cellular evidence for the existence of these distinct complexes is mostly inferred from immunoprecipitation analysis and would benefit from further validation.

Reviewed by eLife

Wound-Induced Syncytia Outpace Mononucleate Neighbors during Drosophila Wound Repair

1. James S White
2. Junmin Hua
3. Jasmine J Su
4. Kaden J Tro
5. Elizabeth M Ruark
6. M Shane Hutson
7. Andrea Page-McCaw

• Curated by eLife

  eLife Assessment

  This important work addresses a very relevant biological question: what is the cellular basis of wound healing? Using the Drosophila pupal notum as a model, the paper provides an elegant, thorough, descriptive characterization of syncytia-driven wound closure using state-of-the-art confocal live imaging of the pupal notum. The authors meticulously characterize the cell-cell fusion events during wound healing and inhibit cell fusion to show to that it is necessary to speed wound closure. In addition, the study provides convincing evidence that cell fusion allows actin resources at be partitioned to the leading edge.

Reviewed by eLife

Detecting Nuclear Pore Complex assembly in living cells

1. Annemiek C Veldsink
2. Jonas S Fischer
3. Hanna M Terpstra
4. Philip J Mannino
5. Eline MF de Lange
6. Sophie Hell
7. Koen J van Benthem
8. Leila J Saba
9. Anton Steen
10. Rifka Vlijm
11. Matthias Heinemann
12. Karsten Weis
13. C Patrick Lusk
14. Liesbeth M Veenhoff

Reviewed by Review Commons