Cell Biology

SpotMAX: a generalist framework for multi-dimensional automatic spot detection and quantification

1. Francesco Padovani
2. Ivana Čavka
3. Ana Rita Rodrigues Neves
4. Cristina Piñeiro López
5. Nada Al-Refaie
6. Leonardo Bolcato
7. Dimitra Chatzitheodoridou
8. Yagya Chadha
9. Xiaofeng A. Su
10. Jette Lengefeld
11. Daphne S. Cabianca
12. Simone Köhler
13. Kurt M. Schmoller

Reviewed by Arcadia Science

Unbiased identification of cell identity in dense mixed neural cultures

1. Sarah De Beuckeleer
2. Tim Van De Looverbosch
3. Johanna Van Den Daele
4. Peter Ponsaerts
5. Winnok H De Vos

• Curated by eLife

  eLife Assessment

  This study presents an important application of high-content image-based morphological profiling to quantitatively and systematically characterize induced pluripotent stem cell-derived mixed neural cultures cell type compositions. Compelling evidence through rigorous experimental and computational validations support new potential applications of this cheap and simple assay.

Reviewed by eLife

Mechanisms of PP2A-Ankle2 dependent nuclear reassembly after mitosis

1. Jingjing Li
2. Xinyue Wang
3. Laia Jordana
4. Éric Bonneil
5. Victoria Ginestet
6. Momina Ahmed
7. Mohammed Bourouh
8. Cristina Mirela Pascariu
9. T Martin Schmeing
10. Pierre Thibault
11. Vincent Archambault

• Curated by eLife

  eLife Assessment

  This is an important study that reports the mechanism by which Ankle2 (LEM4 in humans) interacts with and recruits PP2A and the ER protein Vap33 to promote BAF dephosphorylation and mediate nuclear membrane reformation, using Drosophila as their model. Using Ankle2 mutants, they find that the ER protein Vap33 is key for the normal interphase localisation of Ankle2/LEM4 and also impacts on the function of Ankle2/LEM4 during mitosis. The authors use a variety of complementary techniques and provide convincing evidence to support the claims. The conclusions about the subcellular localization of Ankle2 might be incomplete since they are drawn from overexpression experiments.

Reviewed by eLife

The dependence of shugoshin on Bub1-kinase activity is dispensable for the maintenance of spindle assembly checkpoint response in Cryptococcus neoformans

1. Satya Dev Polisetty
2. Krishna Bhat
3. Kuladeep Das
4. Ivan Clark
5. Kevin G. Hardwick
6. Kaustuv Sanyal

Reviewed by Review Commons

Rho-ROCK liberates sequestered claudin for rapid de novo tight junction formation

1. Yuma Cho
2. Akari Taniguchi
3. Akiharu Kubo
4. Junichi Ikenouchi

• Curated by eLife

  eLife Assessment

  This work is potentially important and largely convincing given the state-of-the-art approaches used to unravel the mechanism underlying the release of Claudins via Rho-mediated activation of Matriptase during tight junction formation. However, there are a few concerns. Addressing the following two major concerns a) showing Matriptase is indeed activated and b) Matriptase inhibition does not interfere with keratinocyte specification, would significantly improve the strength of the evidence. In addition, including quantifications, missing methods, and improving the rigor of the analyses would be helpful.

Reviewed by eLife

Immunosuppression Drugs Exhibit Differential Effects on Endothelial Cell Function

1. Aly Elezaby
2. Ryan Dexheimer
3. David Wu
4. Sze Yu Chan
5. Ian Y. Chen
6. Nazish Sayed
7. Karim Sallam

Reviewed by PREreview

Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

1. Ryosuke Isotani
2. Masaki Igarashi
3. Masaomi Miura
4. Kyoko Naruse
5. Satoshi Kuranami
6. Manami Katoh
7. Seitaro Nomura
8. Toshimasa Yamauchi

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on a potential signaling pathway responsible for the direct effects of nicotine on intestinal stem cell growth and tumorigenesis. The evidence supporting the claims of the authors is solid. This research will be of interest to medical biologists specializing in intestinal tumors.

Reviewed by eLife

The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence

1. Fan Zhang
2. Yuan Wang
3. Luyang Zhang
4. Chunjie Wang
5. Deping Chen
6. Haibo Liu
7. Ren Xu
8. Cole M Haynes
9. Jae-Hyuck Shim
10. Xianpeng Ge

• Curated by eLife

  eLife Assessment

  This important work advances our understanding of CHMP5's role in regulating osteogenesis through its impact on cellular senescence. The evidence supporting the conclusion is mostly convincing, although including additional experiments and discussions would further strengthen the study. This paper holds potential interest for skeletal biologists who study the pathogenesis of age-associated skeletal disorders.

Reviewed by eLife

RGG motif-containing Scd6/LSM14A proteins regulate the translation of specific mRNAs in response to hydroxyurea-induced genotoxic stress

1. Gayatri Mohanan
2. Raju Roy
3. Hélène Malka-Mahieu
4. Swati Lamba
5. Lucilla Fabbri
6. Anusmita Biswas
7. Sylvain Martineau
8. Céline M. Labbé
9. Stéphan Vagner
10. Purusharth I Rajyaguru

Reviewed by Review Commons

Pin4 Links Post-transcriptional and Transcriptional Responses to Glucose Starvation in Yeast

1. Michaela Ristová
2. Katherine Bexley
3. Vadim Shchepachev
4. Atlanta G. Cook
5. David Tollervey

Reviewed by Arcadia Science