Cell Biology

Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor

1. Laura Downie
2. Nuria Ferrandiz
3. Megan Jones
4. Stephen J. Royle

Reviewed by preLights

Golgi compaction facilitates microtubule nucleation to drive adult vertebrate peripheral neuron regeneration

1. Alice E Mortimer
2. Adam J Reid
3. Raman M Das

Reviewed by preLights

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

1. Liyi Wang
2. Shiqi Liu
3. Shu Zhang
4. Yizhen Wang
5. Yanbing Zhou
6. Tizhong Shan

• Curated by eLife

  eLife Assessment

  This revised study provides valuable information on the single nucleus RNA sequencing transcriptome, pathways, and cell types in pig skeletal muscle in response to conjugated linoleic acid (CLA) supplementation. Based on the comprehensive data analyses, the data are considered compelling and provide new insight into the mechanisms underlying intramuscular fat deposition and muscle fiber remodeling. The revised study clarifies major aspects of its methodology and analysis, addresses previous reviewer concerns, and contributes significantly to the understanding of nutritional strategies for fat infiltration in pig muscle.

Reviewed by eLife

Image-based identification and isolation of micronucleated cells to dissect cellular consequences

1. Lucian DiPeso
2. Sriram Pendyala
3. Heather Z Huang
4. Douglas M Fowler
5. Emily M Hatch

• Curated by eLife

  eLife Assessment

  This valuable paper reports image analysis pipelines for the automated segmentation of micronuclei and the detection and sorting of micronuclei-containing cells, which could be powerful tools for researchers studying micronuclei. While the development of the pipelines is solid, a proof-of-principle experiment is not entirely conclusive and leaves open the possibility that additional refinements are required, which would be facilitated by a more detailed explanation of the methods used.

Reviewed by eLife

LAPTM4B Alleviates Pulmonary Fibrosis by Enhancing NEDD4L-Mediated TGF-β Signaling Suppression

1. Kai Xu
2. Xiaoyue Pan
3. Hui Lian
4. Yaxuan Wang
5. Ruyan Wan
6. Zhongzheng Li
7. Xin Pan
8. Yajun Li
9. Juntang Yang
10. Ivan Rosas
11. Lan Wang
12. Guoying Yu

• Curated by eLife

  eLife Assessment

  In this valuable manuscript, the authors propose that the lysosomal protein LAPTM4B plays a role in suppressing the TGF-β/SMAD signaling pathway and suggest that enhancing LAPTM4B function could be a potential therapeutic strategy for alleviating bleomycin-induced lung fibrosis. The findings will be of interest to the lung disease field, and the data presented to support the authors' conclusions is solid. However, it remains unclear whether the suppressive effect of LAPTM4b on idiopathic pulmonary fibrosis is mediated by Nedd4l.

Reviewed by eLife

Reprograming gene expression in hibernating C. elegans involves the IRE-1/XBP-1 pathway

1. Melanie L Engelfriet
2. Yanwu Guo
3. Andreas Arnold
4. Eivind Valen
5. Rafal Ciosk

• Curated by eLife

  eLife Assessment

  This study uses C. elegans, a poikilothermic ("cold-blooded") animal, to investigate the interesting question of how cells and organisms adapt to prolonged exposure to cold temperature. The study employed ribosome profiling and RNAseq analyses and provides a useful inventory of genes changed in cold adapted nematodes. However, the overall conclusions that 1) translation is ongoing at a low rate and 2) IRE mediated transcriptional changes play a significant role in cold adaptation are incompletely supported by the evidence provided. The authors are encouraged to conduct additional bioinformatic analyses and rewrite the manuscript to more accurately reflect the evidence provided.

Reviewed by eLife

Cigarette smoke impairs the endocytotic process in Saccharomyces cerevisiae

1. Aditya Shukla
2. Srimonti Sarkar
3. Alok Kumar Sil

Reviewed by Arcadia Science

Global coordination of protrusive forces in migrating immune cells

1. Patricia Reis-Rodrigues
2. Nikola Canigova
3. Mario J. Avellaneda
4. Florian Gaertner
5. Kari Vaahtomeri
6. Michael Riedl
7. Jack Merrin
8. Robert Hauschild
9. Yoshinori Fukui
10. Alba Juanes Garcia
11. Michael Sixt

Reviewed by preLights

Pharmacologically inducing regenerative cardiac cells by small molecule drugs

1. Wei Zhou
2. Kezhang He
3. Chiyin Wang
4. Pengqi Wang
5. Dan Wang
6. Bowen Wang
7. Han Geng
8. Hong Lian
9. Tianhua Ma
10. Yu Nie
11. Sheng Ding

• Curated by eLife

  eLife Assessment

  This manuscript offers valuable information on the combinatory effect of small molecules, CHIR99021 and A-485 (2C), during the reprogramming of mature cardiomyocytes into regenerative cardiac cells on stimulating cardiac cell regeneration. Although the study used several hESC lines and an in vivo model of myocardial injury to demonstrate the regenerative potential of cardiac cells, the manuscript is still incomplete as several concerns remain unanswered, including the lack of validation of the conclusions from scRNA-seq. It is still unclear how a small fraction of dedifferentiating cardiac cells can offer such broad effects on regeneration both in vitro and in vivo. If validated, this study might unlock potential therapeutic strategies for cardiac regeneration.

Reviewed by eLife

Centrosome Migration and Apical Membrane Formation in Polarized Epithelial Cells: Insights from the MDCK Cyst Model

1. Po-Kai Wang
2. Keng-Hui Lin
3. Tang K Tang

• Curated by eLife

  eLife Assessment

  Wang et al's study addresses an important critical gap in our understanding of de novo epithelial polarization using MDCK cell doublets surrounded by ECM, providing convincing evidence through imaging and depletion studies on the role of conserved polarity proteins and the centrosome during this process. While the authors propose a clear hierarchical model, there is a need for further exploration of how microtubule organization contributes to this process. Specifically, live cell imaging of microtubules under mutants and their included ECM conditions, along with a more precise temporal mapping of microtubule dynamics in relation to proteins like Gp135, would strengthen the study's conclusions.

Reviewed by eLife