Cell Biology

Sphingolipid imbalance aggravates tau pathology by endomembrane rigidification and rupture

1. Jessica Tittelmeier
2. Carl Alexander Sandhof
3. Nicole Martin
4. Deike El-Kabarity
5. Soki-Bradel Ngonza-Nito
6. Ronald Melki
7. Carmen Nussbaum-Krammer

• Curated by eLife

  eLife Assessment

  This valuable study addresses the role of sphingolipid metabolism in maintaining endolysosomal membrane integrity and its impact on tau pathology in Caenorhabditis elegans and human cell culture models. The methods are solid and the proposed mechanisms are conceivable. However, the current evidence is incomplete and could be strengthened, due to reliance on imaging data and insufficient biochemical validation. The work will be of broad interest to cell biologists and biologists working on Alzheimer's disease and related proteinopathies.

Reviewed by eLife

Intra-manchette transport employs both microtubule and actin tracks

1. Jo H. Judernatz
2. Laura Pérez Pañeda
3. Tereza Kadavá
4. Albert J. R. Heck
5. Tzviya Zeev-Ben-Mordehai

Reviewed by Review Commons

Succinate Dehydrogenase loss causes cascading metabolic effects that impair pyrimidine biosynthesis

1. Madeleine L. Hart
2. Kristian Davidsen
3. Serwah Danquah
4. Eric Zheng
5. David Sokolov
6. Lucas B. Sullivan

Reviewed by preLights

GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity in Cancer

1. Yuki Kito
2. Tania J González-Robles
3. Sharon Kaisari
4. Juhee Pae
5. Sheena Faye Garcia
6. Juliana Ortiz-Pacheco
7. Beatrix Ueberheide
8. Ruth Lehmann
9. Antonio Marzio
10. Gergely Rona
11. Michele Pagano

• Curated by eLife

  eLife Assessment

  This study identifies 53BP1 as an interaction partner of GMCL1 (a likely CUL3 substrate receptor). The study seeks to link this finding to regulation of the mitotic surveillance pathway and paclitaxel resistance in cancer. The evidence for these claims is currently inadequate; concerns include the use of cell lines that have been reported to lack the mitotic surveillance pathway, insufficient consideration of paclitaxel mechanisms of action, and an overinterpretation of correlative results.

Reviewed by eLife

Extracellular vesicle-mediated release of bis(monoacylglycerol)phosphate is regulated by LRRK2 and Glucocerebrosidase activity

1. Elsa Meneses-Salas
2. Marianna Arnold
3. Moises Castellá
4. Frank Hsieh
5. Ruben Fernández-Santiago
6. Mario Ezquerra
7. Alicia Garrido
8. María-José Martí
9. Carlos Enric
10. Suzanne R Pfeffer
11. Kalpana Merchant
12. Albert Lu

• Curated by eLife

  eLife Assessment

  This useful study presents the potentially interesting concept that LRRK2 regulates cellular BMP levels and their release via extracellular vesicles, with GCase activity further modulating this process in mutant LRRK2-expressing cells. However, the evidence supporting the conclusions remains incomplete, and certain statistical analyses are inadequate. This work would be of interest to cell biologists working on Parkinson's disease.

Reviewed by eLife

Intestinal stem cell renewal controlled by capillary morphogenesis gene 2 following injury

1. Lucie Bracq
2. Audrey Chuat
3. Béatrice Kunz
4. Olivier Burri
5. Romain Guiet
6. F. Gisou van der Goot

Reviewed by Review Commons

The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence

1. Fan Zhang
2. Yuan Wang
3. Luyang Zhang
4. Chunjie Wang
5. Deping Chen
6. Haibo Liu
7. Ren Xu
8. Cole M Haynes
9. Jae-Hyuck Shim
10. Xianpeng Ge

• Curated by eLife

  eLife Assessment

  This important work advances our understanding of CHMP5's role in regulating osteogenesis through its impact on cellular senescence. The evidence supporting the conclusion is convincing and the revised manuscript is largely improved. This paper holds potential interest for skeletal biologists who study the pathogenesis of age-associated skeletal disorders.

Reviewed by eLife

Aging-associated Increase of GATA4 levels in Articular Cartilage is Linked to Impaired Regenerative Capacity of Chondrocytes and Osteoarthritis

1. Meagan J. Makarczyk
2. Yiqian Zhang
3. Alyssa Aguglia
4. Olivia Bartholomew
5. Sophie Hines
6. Suyash Sinkar
7. Silvia Liu
8. Craig Duvall
9. Hang Lin

• Curated by eLife

  eLife Assessment

  This study presents an important finding on the role of GATA4 in aging and OA-associated cartilage pathology. The evidence supporting the conclusions is compelling, with rigorous in vitro and in vivo data. The work will be of broad interest to cell biologists and orthopedic clinicians.

Reviewed by eLife

Clearance of protein aggregates during cell division

1. Shoukang Du
2. Yuhan Wang
3. Bowen Chen
4. Shuangshuang Xie
5. Kuan Yoow Chan
6. David C Hay
7. Ting Gang Chew

• Curated by eLife

  eLife Assessment

  How misfolded proteins are segregated and cleared is a significant question in cell biology, since clearance of these aggregates can protect against pathologies that may otherwise arise. The authors discover a cell cycle stage-dependent clearing mechanism that involves the ER chaperone BiP, the proteosome, and CDK inactivation, but is curiously independent of the anaphase promoting complex (APC). These are valuable and interesting new observations, and the evidence supporting these claims is solid.

Reviewed by eLife

Profiling the physiological impact of aberrant folded-state protein filamentation in cells

1. Tal Levin
2. Hector Garcia-Seisdedos
3. Arseniy Lobov
4. Matthias Wojtynek
5. Alexander Alexandrov
6. Ghil Jona
7. Dikla Levi
8. Ohad Medalia
9. D. Emmanuel Levy

Reviewed by Review Commons