Cell Biology

Canonical and phosphoribosyl ubiquitination coordinate to stabilize a proteinaceous structure surrounding the Legionella-containing vacuole

1. Adriana Steinbach
2. Chetan Mokkapati
3. Shaeri Mukherjee

• Curated by eLife

  eLife Assessment

  The authors describe the important finding that the Legionella-containing vacuole is surrounded by a dense ubiquitin "cloud" that is highly resistant to detergent extraction. The study provides compelling evidence that this structure is generated through a combination of canonical ubiquitination mediated by the SidC Legionella ligase and phosphoribosyl-ubiquitination mediated by the SidE family of Legionella ligases. These findings provide important insight into how Legionella remodels the host vacuolar environment through complex ubiquitin modifications.

Reviewed by eLife

Enteropathogenic E. coli-mediated Fast and Coordinated Ca2+ responses regulate NF-κB activation

1. Fangrui Guo
2. Roberto Ornelas Guevara
3. Linda Oussaedine
4. Geneviève Dupont
5. Laurent Combettes
6. Guy Tran Van Nhieu

• Curated by eLife

  eLife Assessment

  This study reports important advances in our understanding of how enteropathogenic E. coli (EPEC) interacts at the intestinal interface. Compelling data describe a novel model of spatially coordinated calcium signaling to modulate NF-kB activation. These findings, which integrate imaging, genetics, and computational modeling, provide a new way to consider host-pathogen interactions in EPEC infections that may lead to improved therapies.

Reviewed by eLife

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

1. Rainer Ettelt
2. Ana-Maria Sandru
3. Georg Vucak
4. Sebastian Didusch
5. Biljana Riemelmoser
6. Karin Ehrenreiter
7. Markus Hartl
8. Lukas A. Huber
9. Manuela Baccarini

Reviewed by Review Commons

Contractile perinuclear actomyosin network promotes peripheral and polar chromosome interaction with the mitotic spindle

1. Nooshin Sheidaei
2. John K Eykelenboom
3. Zuojun Yue
4. Graeme Ball
5. Alexander JR Booth
6. Tomoyuki U Tanaka

• Curated by eLife

  eLife Assessment

  This important study demonstrates that a perinuclear actomyosin network, present in some types of human cells, facilitates kinetochore-spindle attachment of chromosomes in unfavorable locations, thereby reducing their missegregation rate. This actomyosin network and its general role have been studied previously, but this study convincingly clarifies the underlying mechanism and expands the investigation to additional cell lines. The results are relevant to understanding chromosome missegregation in cancer cells.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Muscular dystrophy-associated lamin variants disrupt cellular organization through a nucleolar-ribosomal axis controlling cytoplasmic macromolecular crowding

1. Xiangyi Ding
2. Sweta Kumari
3. Ellen Gregory
4. Daniel A. Starr
5. G. W. Gant Luxton

Reviewed by Arcadia Science

Noncanonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy

1. Hao Chen
2. Haocheng Wang
3. Yuning Lu
4. Peng Chen
5. Zhongfan Zheng
6. Tao Zhang
7. Jiou Wang

• Curated by eLife

  eLife Assessment

  The revised version of the paper demonstrates that a genetic code expansion to tag two ALS proteins associated with stress granules is useful and convincingly evaluates the utility of the genetic code expansion in this context. The data is solid and demonstrates the feasibility of using ANAP-fluorescence for live cell imaging.

Reviewed by eLife

Syntaxin11 Deficiency Inhibits CRAC Channel Priming to Suppress Cytotoxicity and Gene Expression in T Lymphocytes

1. Sritama Datta
2. Abhikarsh Gupta
3. Kunal Mukesh Jagetiya
4. Resmi Bera
5. Vikas Tiwari
6. Atharva Rahul Yande
7. Megumi Yamashita
8. Abdul Rishad
9. Vishal Malik
10. Sreejith Raran-Kurussi
11. Sandra Ammann
12. Mohammad Shahrooei
13. Kalyaneswar Mandal
14. Ramanathan Sowdhamini
15. Murali Prakriya
16. Adish Dani
17. Monika Vig

• Curated by eLife

  eLife Assessment

  This study reports a novel function for syntaxin 11, a specialized SNARE protein critical for the immune system whose mutations cause familial hemophagocytic lymphohistiocytosis type 4. The data convincingly show that depletion of STX11 impairs store-operated calcium entry in Jurkat T cells and that this defect is recapitulated in primary cells from a patient suffering from the disease; the authors further show that the syntaxin interacts with the pore subunit of the ORAI1 channel and propose that it primes the channel by promoting the assembly of multimers before activation by its endogenous ligand, the ER Ca2+ sensing protein STIM1. This is a conceptually important claim that challenges the prevailing view that all structural transitions in ORAI1 are STIM-driven. The data are high-quality and broadly consistent with the interpretation, but alternative mechanisms for the defects are not considered; additional work should rule out vesicular trafficking, discuss other mechanisms, and address methodological issues.

Reviewed by eLife

The functional maturation of mouse spermatozoa is underpinned by global remodeling of the cellular phosphoproteome

1. David A. Skerrett-Byrne
2. Amanda L. Anderson
3. Raffaele Teperino
4. Nathan D. Burke
5. Elizabeth G. Bromfield
6. Matthew D. Dun
7. Valérie Gailus-Durner
8. Helmut Fuchs
9. Susan Marschall
10. Martin Hrabě de Angelis
11. Sean J. Humphrey
12. Brett Nixon

Reviewed by Review Commons

Cell size modulates ferroptosis susceptibility

1. Evgeny Zatulovskiy
2. Magdalena B Murray
3. Shuyuan Zhang
4. Scott J Dixon
5. Jan M Skotheim

• Curated by eLife

  eLife Assessment

  This important study highlights how cell size influences various cellular responses, with a particular focus on ferroptosis. The evidence presented is convincing, employing multiple model systems and experimental approaches to support the conclusions. This work will be of significant interest to the fields of cell size, ferroptosis, and cancer biology.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

The insulin / IGF axis is critically important for controlling gene transcription in the podocyte

1. Jennifer A Hurcombe
2. Lusyan Dayalan
3. Fern Barrington
4. Frédéric Burdet
5. Lan Ni
6. Joseph T Coward
7. Mark Ibberson
8. Paul T Brinkkoetter
9. Martin Holzenberger
10. Aaron Jeffries
11. Sebastian Oltean
12. Gavin I Welsh
13. Richard JM Coward

• Curated by eLife

  eLife Assessment

  This valuable study used genetic and pharmacological manipulations of insulin/IGF signaling to address the role of insulin/IGF axis in the function of renal glomerular podocyte. Solid data are presented to demonstrate that co-inhibition of insulin/IGF signaling in podocytes led to aberrant splicing of mRNAs, which could contribute to the loss of podocytes in vitro and in vivo in mice. In light of the fact that IR/IGF-1R signaling are critically required for normal development and growth in multiple cells and organs, the lack of the assessment of developmental phenotype of podocytes in the mouse model limits the interpretation of the data.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife