Cell Biology

Human receptive endometrial assembloid for deciphering the implantation window

1. Yu Zhang
2. Rusong Zhao
3. Chaoyan Yang
4. Jinzhu Song
5. Peishu Liu
6. Yan Li
7. Boyang Liu
8. Tao Li
9. Changjian Yin
10. Minghui Lu
11. Zhenzhen Hou
12. Chuanxin Zhang
13. Zi-Jiang Chen
14. Keliang Wu
15. Han Zhao

• Curated by eLife

  eLife Assessment

  This important study reports an endometrial organoid culture system mimicking the window of implantation. The evidence supporting the conclusion drawn is convincing. The data will be of interest to embryologists and investigators working on reproductive biology and medicine.

Reviewed by eLife

A bipartite, mutation-tolerant NLS regulates interaction of ΔNp63α with importin alpha, nuclear transport and transcriptional activity

1. Anna Demarinis
2. Sara Esmaeili
3. Crystall M. D. Swarbrick
4. Simone Ranno
5. Silvia Pavan
6. Jade K. Forwood
7. Brian P. McSharry
8. Martin Pal
9. Enzo Di Iorio
10. Gualtiero Alvisi

Reviewed by Review Commons

Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex

1. Stefano Sellitto
2. Davide Caredio
3. Matteo Bimbati
4. Giovanni Mariutti
5. Martina Cerisoli
6. Lukas Frick
7. Vangelis Bouris
8. Carlos Omar Oueslati Morales
9. Dalila Laura Vena
10. Sandesh Neupane
11. Federico Baroni
12. Kathi Ging
13. Jiang-An Yin
14. Elena De Cecco
15. Andrea Armani
16. Adriano Aguzzi

Reviewed by Review Commons

Unremodeled plasma membrane GPI-anchored proteins trigger aberrant clathrin-mediated endocytosis

1. Li Chen
2. David K. Banfield

Reviewed by PREreview

Probing metazoan polyphosphate biology using Drosophila reveals novel and conserved polyP functions

1. Sunayana Sarkar
2. Harsha Sharma
3. SK Yasir Hosen
4. Jayashree S Ladke
5. Sandra Moser
6. Deepa Balasubramanian
7. Sreejith Raran-Kurussi
8. Henning Jessen
9. Rashna Bhandari
10. Manish Jaiswal

• Curated by eLife

  eLife Assessment

  Studying the biological roles of polyphosphates in metazoans has been a longstanding challenge to the field given that the polyP synthase has yet to be discovered in metazoans. This important study capitalizes on the sophisticated genetics available in the Drosophila system and uses a combination of methodologies to start to tease apart how polyphosphate participates in Drosophila development and in the clotting of Drosophila hemolymph. The data validating one of these tools (cyto-FLYX ) are solid and well-documented and they will open up a field of research into the functional roles of polyP in a metazoan model. The other tools for tissue specific knockdown of polyP (Mito-FLYX, ER-FLYX, and Nuc-FLYX) have not yet been validated but will be invaluable to the field when they are.

Reviewed by eLife

PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progression

1. Jimena Druker
2. Hao Jiang
3. Dilem Shakir
4. Fraser Child
5. Vanesa Alvarez
6. Melpomeni Platani
7. Andrea Corno
8. Constance Alabert
9. Adrian T Saurin
10. Jason R Swedlow
11. Sonia Rocha
12. Angus I Lamond

• Curated by eLife

  eLife Assessment

  This compelling work describes how the cell cycle-regulating phosphatase subunit, RepoMan, is regulated by the oxygen-dependent, metabolite-sensing hydroxylase PHD1. The characterisation of how proline hydroxylation alters signalling at the molecular and cellular level provides important evidence to enhance our understanding of how 2-oxoglutarate-dependent dioxygenases influence the cell cycle and mitosis.

Reviewed by eLife

Metabolite import via SLC33A1 enables ATF6 activation by endoplasmic reticulum stress

1. Ginto George
2. Heather P. Harding
3. Richard Kay
4. David Ron
5. Adriana Ordóñez

Reviewed by Review Commons

Large-scale identification of plasma membrane repair proteins revealed spatiotemporal cellular responses to plasma membrane damage

1. Yuta Yamazaki
2. Keiko Kono

• Curated by eLife

  eLife Assessment

  This work provides an important resource identifying 72 proteins as novel candidates for plasma membrane and/or cell wall damage repair in budding yeast, and describes the temporal coordination of exocytosis and endocytosis during the repair process. The data are convincing; however, additional experimental validation will better support the claim that repair proteins shuttle between the bud tip and the damage site.

Reviewed by eLife

Linking Germline Telomere Removal to Global Programmed DNA Elimination in Tetrahymena Genome Differentiation

1. Kohei Nagao
2. Kazufumi Mochizuki

• Curated by eLife

  eLife Assessment

  This important study reveals intriguing connections between chromosome breakage and DNA elimination during programmed genome rearrangement in the ciliate Tetrahymena thermophila. By developing a novel FISH approach that distinguishes germline and somatic telomeres, the authors provide compelling evidence that chromosome breakage removes germline telomeres along with hundreds of kilobases of germline-limited sequences. By disrupting a single chromosome breakage site, they further showed that DNA elimination was globally affected, which opens up a new direction for mechanistic studies. Thus, this work reveals additional similarity between the programmed DNA elimination in ciliates and nematodes that underlies the transition from germline to somatic telomeres.

Reviewed by eLife

Fission yeast cells use distinct cell size control mechanisms for size adaptation to osmotic, oxidative, or low glucose conditions

1. Elena J. Cabral
2. Pablo Andres
3. Geraldin Argandona
4. Paige Duggan
5. Benjamin M. Kuran
6. Kristi E. Miller

Reviewed by Review Commons