Cancer Biology

Spatially defined multicellular functional units in colorectal cancer revealed from single cell and spatial transcriptomics

1. Inbal Avraham-Davidi
2. Simon Mages
3. Johanna Klughammer
4. Noa Moriel
5. Shinya Imada
6. Matan Hofree
7. Evan Murray
8. Jonathan Chen
9. Karin Pelka
10. Arnav Mehta
11. Genevieve M. Boland
12. Toni Delorey
13. Leah Caplan
14. Danielle Dionne
15. Robert Strasser
16. Jana Lalakova
17. Anezka Niesnerova
18. Hao Xu
19. Morgane Rouault
20. Itay Tirosh
21. Nir Hacohen
22. Fei Chen
23. Omer Yilmaz
24. Jatin Roper
25. Orit Rozenblatt-Rosen
26. Mor Nitzan
27. Aviv Regev

• Curated by eLife

  eLife Assessment

  This work presents a useful resource combining scRNA-seq and spatial transcriptomics studies to map mouse pre-clinical models of colorectal cancer, identifying distinct cellular programs and microenvironments that could enhance patient stratification and therapeutic approaches in colorectal cancer. While the novelty of the biological findings remains limited and incompletely supported by the evidence provided in the manuscript, the data were collected and analyzed using a validated methodology that will be of interest to the community in future studies.

Reviewed by eLife

In vivo targeted and deterministic single-cell malignant transformation

1. Pierluigi Scerbo
2. Benjamin Tisserand
3. Marine Delagrange
4. Héloise Debare
5. David Bensimon
6. Bertrand Ducos

• Curated by eLife

  eLife Assessment

  This important study employs an optogenetics approach aimed at activating oncogene (KRASG12V) expression in a single somatic cell, with a focus on following the progression of activated cell to examine tumourigenesis probabilities under altered tissue environments. Although the description of the methodologies applied is incomplete, the authors propose a mechanism whereby reactivation of re-programming factors correlates with the increased likelihood of a mutant cell undergoing malignant transformation. This work will be of interest to developmental and cancer biologists, especially in relation to the genetic tools described.

Reviewed by eLife

Notch signaling maintains a progenitor-like subclass of hepatocellular carcinoma

1. Kerstin Seidel
2. Robert Piskol
3. Thi Thu Thao Nguyen
4. Amy Shelton
5. Charisa Cottonham
6. Cecile C de la Cruz
7. Joseph Castillo
8. Jesse Garcia
9. Udi Segal
10. Mark Merchant
11. Yeqing Angela Yang
12. Jasmine Chen
13. Musa Ahmed
14. Alexis Scherl
15. Rajesh Vij
16. Lluc Mosteiro
17. Yan Wu
18. Zora Modrusan
19. Ciara Metcalfe
20. Chris Siebel

• Curated by eLife

  eLife Assessment

  In this valuable study, Seidel et al. identify and characterize a novel subset of hepatocellular carcinoma patient-derived xenograft models defined by active Jagged 1-Notch2 signaling and a distinctive progenitor-like gene expression profile. Within the limitations of the PDX system they used, their methods are state-of-the-art, their data are strong and believable, and their conclusions are convincing. However, the ability to identify HCC patients that might respond is limited, and the mechanistic assessment downstream of JAG1/NOTCH2 is relatively descriptive. Some additional clarifications and experiments would strengthen the paper.

Reviewed by eLife

STAMBPL1 activates the GRHL3/HIF1A/VEGFA axis through interaction with FOXO1 to promote angiogenesis in triple-negative breast cancer

1. Huan Fang
2. Huichun Liang
3. Chuanyu Yang
4. Dewei Jiang
5. Qianmei Luo
6. Wen-Ming Cao
7. Huifeng Zhang
8. Ceshi Chen

• Curated by eLife

  eLife Assessment

  The study conducted by Fang et al. offers significant and fundamental insights, notably enhancing our understanding of angiogenesis. While some of the claims are supported by convincing experimental approaches, others lack sufficient validation. Additionally, there are instances where critical experimental controls appear to be absent.

Reviewed by eLife

Propionyl-CoA carboxylase subunit B regulates anti-tumor T cells in a pancreatic cancer mouse model

1. Han V Han
2. Richard Efem
3. Barbara Rosati
4. Kevin Lu
5. Sara Maimouni
6. Ya-Ping Jiang
7. Valeria Montoya
8. Ando Van Der Velden
9. Wei-Xing Zong
10. Richard Z Lin

• Curated by eLife

  eLife assessment

  The significance of the findings is valuable, with implications for immunotherapy design in pancreatic ductal adenocarcinoma. The evidence was considered incomplete and partially supportive of the major claims.

Reviewed by eLife

Spatial multi-omics defines a shared glioblastoma infiltrative signature at the resection margin

1. Balagopal Pai
2. Susana Isabel Ramos
3. Wan Sze Cheng
4. Tanvi Joshi
5. Gabrielle Price
6. Jessica Tome-Garcia
7. German Nudelman
8. Sanjana Shroff
9. Kristin Beaumont
10. Yong Raymund
11. Robert Sebra
12. Elena Zaslavsky
13. Nadejda Mincheva Tsankova

Reviewed by PREreview

Tks5 interactome reveals ER-associated machinery translation in invadosomes

1. Léa Normand
2. Benjamin Bonnard
3. Margaux Sala
4. Sylvaine Di-Tommaso
5. Cyril Dourthe
6. Anne-Aurélie Raymond
7. Jean-William Dupuy
8. Luc Mercier
9. Jacky G. Goetz
10. Violaine Moreau
11. Elodie Henriet
12. Frédéric Saltel

Reviewed by Review Commons

Interplay of YEATS2 and GCDH mediates histone crotonylation and drives EMT in head and neck cancer

1. Deepak Pant
2. Parik Kakani
3. Rushikesh Joshi
4. Shruti Agrawal
5. Atul Samaiya
6. Sanjeev Shukla

• Curated by eLife

  eLife Assessment

  These useful findings assigned a novel functional implication of histone acylation, crotonylation. Although the mechanistic insights have been provided in great detail regarding the role of the YEATS2-GCDH axis in modulating EMT in HNC, the strength of evidence for the manuscript is incomplete. The patient cohort is very small, with just 10 patients; to establish a significant result the cohort size should be increased. Furthermore, the functional implication of p300 is also to be looked into.

Reviewed by eLife

Loss of ZNRF3/RNF43 Unleashes EGFR in Cancer

1. Fei Yue
2. Amy T Ku
3. Payton D Stevens
4. Megan N Michalski
5. Weiyu Jiang
6. Jianghua Tu
7. Zhongcheng Shi
8. Yongchao Dou
9. Yi Wang
10. Xin-Hua Feng
11. Galen Hostetter
12. Xiangwei Wu
13. Shixia Huang
14. Noah F Shroyer
15. Bing Zhang
16. Bart O Williams
17. Qingyun Liu
18. Xia Lin
19. Yi Li

• Curated by eLife

  eLife Assessment

  This manuscript presents solid evidence suggesting that the loss of ZNRF3 and RNF43, two E3 ubiquitin ligases, leads to dysregulation of EGFR signaling in cancer. The authors propose that EGFR is a direct substrate of ZNRF3/RNF43. While the authors provide immunoprecipitation data showing increased detection of ubiquitinated species, this evidence does not definitively establish that EGFR itself is ubiquitinated by RNF43/ZNRF3. The absence of direct evidence for EGFR ubiquitination is a major limitation, although the findings are useful as they may provide novel insights into the mechanisms underlying EGFR-driven cancers and open new therapeutic avenues.

Reviewed by eLife

Systemic and local chronic inflammation and hormone disposition promote a tumor-permissive environment for breast cancer in older women

1. Neil Carleton
2. Sanghoon Lee
3. Ruxuan Li
4. Jian Zou
5. Daniel D Brown
6. Jagmohan Hooda
7. Alexander Chang
8. Rahul Kumar
9. Linda R Klei
10. Lora H Rigatti
11. Joseph Newsome
12. Dixcy Jaba Sheeba John Mary
13. Jennifer M Atkinson
14. Raymond E West
15. Thomas D Nolin
16. Patrick J Oberly
17. Ziyu Huang
18. Donald Poirier
19. Emilia J Diego
20. Peter C Lucas
21. George Tseng
22. Michael T Lotze
23. Priscilla F McAuliffe
24. Ioannis K Zervantonakis
25. Steffi Oesterreich
26. Adrian V Lee

Reviewed by PREreview