Biophysics

Correlative all-optical quantification of mass density and mechanics of subcellular compartments with fluorescence specificity

1. Raimund Schlüßler
2. Kyoohyun Kim
3. Martin Nötzel
4. Anna Taubenberger
5. Shada Abuhattum
6. Timon Beck
7. Paul Müller
8. Shovamaye Maharana
9. Gheorghe Cojoc
10. Salvatore Girardo
11. Andreas Hermann
12. Simon Alberti
13. Jochen Guck

• Curated by eLife

  Evaluation Summary:

  In this interesting study, the authors combined Brillouin microscopy with Optical Diffraction Tomography and epi-fluorescence imaging to investigate physical properties of biological materials including nucleoplasm, cytoplasm, phase-separated organelles, and adipocytes. The results are largely convincing and offer interesting insights into the material properties of these subcellular structures.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

A mechanosensing mechanism controls plasma membrane shape homeostasis at the nanoscale

1. Xarxa Quiroga
2. Nikhil Walani
3. Andrea Disanza
4. Albert Chavero
5. Alexandra Mittens
6. Francesc Tebar
7. Xavier Trepat
8. Robert G Parton
9. María Isabel Geli
10. Giorgio Scita
11. Marino Arroyo
12. Anabel-Lise Le Roux
13. Pere Roca-Cusachs

• Curated by eLife

  Evaluation Summary:

  When a cell undergoes rapid shrinking, excess plasma membrane becomes available. The authors show that excess plasma membrane forms very small bleb-like evaginations that disappear after a few minutes. They show a new role for the I-BAR protein IRSp53 and Arp2/3-dependent actin polymerization which surprisingly leads to the flattening of the bud instead of its growth, as it is the case in filopodial protrusions. This manuscript will be of general interest to cell biologists working on membrane-cortex interactions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Mechanism of CFTR correction by type I folding correctors

1. Karol Fiedorczuk
2. Jue Chen

• Curated by Biophysics Colab

  Endorsement statement (27 April 2022)

  The preprint by Fiedorczuk and Chen presents structures of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel in complex with type I correctors, a class of drug currently used to treat cystic fibrosis by targeting CFTR folding and stability. The strength of the paper lies in the consistency of the structural data with maturation and binding assays, as well as with much of the existing literature. Overall, the work represents a rigorous investigation of the mechanism of these drugs, and will be of interest to those who study cystic fibrosis, protein folding, and drug design.

  (This endorsement refers to version 1 of this preprint, which was peer reviewed by Biophysics Colab.)

Reviewed by Biophysics Colab

Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel

1. Vishal R. Patel
2. Arturo M. Salinas
3. Darong Qi
4. Shipra Gupta
5. David J. Sidote
6. Marcel P. Goldschen-Ohm

• Curated by Biophysics Colab

  Endorsement statement (30 August 2021)

  The preprint by Patel et al. describes the development of a single molecule approach for studying individual ligand binding events in membrane proteins within native lipid environments. The approach represents an elegant way to investigate the dynamics of ligand binding, and potential relationships with conformational changes, in molecules embedded within physiological membranes. The work makes an important contribution that will be of interest to scientists working on molecular mechanisms in ion channels and other membrane proteins.

  (This endorsement by Biophysics Colab refers to version 2 of this preprint, which has been revised in response to peer review of version 1.)

Reviewed by Biophysics Colab

Quantitative theory for the diffusive dynamics of liquid condensates

1. Lars Hubatsch
2. Louise M Jawerth
3. Celina Love
4. Jonathan Bauermann
5. TY Dora Tang
6. Stefano Bo
7. Anthony A Hyman
8. Christoph A Weber

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to chemists and biologists studying complex coacervate systems, including biomolecular condensates. Its model provides a new way of obtaining diffusion properties inside and outside the condensates without the necessity of nontrivial assumptions. The model's capability is well presented by applying to experimental data and through further investigating the model through simulations.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Strikingly Different Roles of SARS-CoV-2 Fusion Peptides Uncovered by Neutron Scattering

1. Andreas Santamaria
2. Krishna C. Batchu
3. Olga Matsarskaia
4. Sylvain F. Prévost
5. Daniela Russo
6. Francesca Natali
7. Tilo Seydel
8. Ingo Hoffmann
9. Valérie Laux
10. Michael Haertlein
11. Tamim A. Darwish
12. Robert A. Russell
13. Giacomo Corucci
14. Giovanna Fragneto
15. Armando Maestro
16. Nathan R. Zaccai

Reviewed by ScreenIT

Allosteric Regulation of 3CL Protease of SARS-CoV-2 and SARS-CoV Observed in the Crystal Structure Ensemble

1. Akinori Kidera
2. Kei Moritsugu
3. Toru Ekimoto
4. Mitsunori Ikeguchi

Reviewed by ScreenIT

Structure-based screening of drug candidates targeting the SARS-CoV-2 envelope protein

1. Xin Xia
2. Yuwei Zhang
3. Songling Li
4. Hengwei Lin
5. Zhiqiang Yan

Reviewed by ScreenIT

Structural dynamics of SARS-CoV-2 nucleocapsid protein induced by RNA binding

1. Helder Veras Ribeiro Filho
2. Gabriel Ernesto Jara
3. Fernanda Aparecida Heleno Batista
4. Gabriel Ravanhani Schleder
5. Celisa Caldana Tonoli
6. Adriana Santos Soprano
7. Samuel Leite Guimarães
8. Antonio Carlos Borges
9. Alexandre Cassago
10. Marcio Chaim Bajgelman
11. Rafael Elias Marques
12. Daniela Barreto Barbosa Trivella
13. Kleber Gomes Franchini
14. Ana Carolina Migliorini Figueira
15. Celso Eduardo Benedetti
16. Paulo Sergio Lopes de Oliveira

Reviewed by ScreenIT

Defective ORF8 dimerization in delta variant of SARS CoV2 leads to abrogation of ORF8 MHC-I interaction and overcome suppression of adaptive immune response

1. Armi M Chaudhari
2. Indra Singh
3. Madhvi Joshi
4. Amrutlal Patel
5. Chaitanya Joshi

Reviewed by ScreenIT