Structural Basis of the Main Proteases of Coronavirus Bound to Drug Candidate PF-07321332

  1. Jian Li
  2. Cheng Lin
  3. Xuelan Zhou
  4. Fanglin Zhong
  5. Pei Zeng
  6. Yang Yang
  7. Yuting Zhang
  8. Bo Yu
  9. Xiaona Fan
  10. Peter J. McCormick
  11. Rui Fu
  12. Yang Fu
  13. Haihai Jiang
  14. Jin Zhang

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Abstract

The current pandemic of multiple variants has created an urgent need for effective inhibitors of SARS-CoV-2 to complement vaccine strategies. PF-07321332, developed by Pfizer, is the first orally administered coronavirus-specific main protease inhibitor approved by the FDA.

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  1. Version published to 10.1128/jvi.02013-21
  2. ScreenIT

    SciScore for 10.1101/2021.11.05.467529: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Recombinant DNA
    SentencesResources
    Synthesized genes were subcloned into the pET-28a vector.
    pET-28a
    suggested: RRID:Addgene_44242)
    Software and Algorithms
    SentencesResources
    The IC50 of compounds was determined by plotting the initial velocity against various concentrations of testing inhibitor by using the dose-response curve in GraphPad Prism software.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    The structures were determined by molecular replacement with PHENIX software.
    PHENIX
    suggested: (Phenix, RRID:SCR_014224)
    The program Coot was used to rebuild the initial model.
    Coot
    suggested: (Coot, RRID:SCR_014222)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.11.05.467529v1 on bioRxiv