Biophysics

An in silico testbed for fast and accurate MR labeling of orthopedic implants

1. Gregory M Noetscher
2. Peter J Serano
3. Marc Horner
4. Alexander Prokop
5. Jonathan Hanson
6. Kyoko Fujimoto
7. James Brown
8. Ara Nazarian
9. Jerome Ackerman
10. Sergey N Makaroff

• Curated by eLife

  eLife assessment

  This manuscript will provide a valuable method to evaluate the safety of MR in patients with orthopaedic implants, which is required in clinics. A strength of the work is that the in-silicon testbed is solid, based on the widely available human project, and validated. In addition, the toolbox will be open for clinical practice.

Reviewed by eLife

Physical basis of the cell size scaling laws

1. Romain Rollin
2. Jean-François Joanny
3. Pierre Sens

• Curated by eLife

  eLife assessment

  This theoretical work deals with the problem of homeostasis of protein density within cells, relying on the Pump and Leak model. The model makes predictions both for growing and senescent cells, which they compare to experimental data on budding yeast. The work extends previous works and makes biologically-relevant predictions, which will be of interest to both theorists and experimentalists interested in cell physiology.

Reviewed by PREreview, eLife

Approximating Projections of Conformational Boltzmann Distributions with AlphaFold2 Predictions: Opportunities and Limitations

1. Benjamin P. Brown
2. Richard A. Stein
3. Jens Meiler
4. Hassane S. Mchaourab

Reviewed by PREreview

Allosteric regulation of kinase activity in living cells

1. Shivani Sujay Godbole
2. Nikolay V Dokholyan

• Curated by eLife

  eLife assessment

  One of the most promising strategies in development of drugs targeting kinases is provided by using allosteric control that allows specific regulation and study of kinase function without directly targeting the active site. This important paper reviews convincingly the current repertoire of tools for regulating the activity of protein kinases with the ultimate goal of developing novel approaches in treating diseases associated with signal dysregulation.

Reviewed by eLife

Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states

1. David Yin-wei Lin
2. Lauren E Kueffer
3. Puneet Juneja
4. Thomas E Wales
5. John R Engen
6. Amy H Andreotti

• Curated by eLife

  eLife assessment

  BTK, a TEC-family tyrosine kinase activated by the B-cell antigen receptor, contains a variety of regulatory domains and it is subject to complex regulation by membrane phospholipids, protein ligands, phosphorylation, and dimerization. This study presents convincing evidence, utilizing various biophysical techniques, to support a model for BTK activation that will be valuable for the field. Overall, the study enhances the understanding of BTK's activation mechanism, autoinhibition, and allosteric control, challenging previous assumptions about BTK.

Reviewed by eLife

A role for JAK2 in mediating cell surface GHR-PRLR interaction

1. Chen Chen
2. Jing Jiang
3. Tejeshwar C. Rao
4. Tatiana T. Marquez Lago
5. Stuart J. Frank
6. André Leier

• Curated by eLife

  eLife assessment

  This is an important study that characterizes a surprising interaction between two different cytokine/hormone receptors using nanoscale resolution (dSTORM) microscopy. The study provides solid evidence that the interaction is ligand-dependent, and is mediated by the receptor-associated intracellular signalling molecule JAK2. While at present limited to growth hormone and prolactin receptors in a limited number of cell lines, there are potentially broad implications for cytokine signalling, as such JAK2-mediated interactions could occur between a range of different cytokines. Moreover, the specific hormone interactions shown in the manuscript may have important implications for understanding how these hormones can have differential effects in breast cancer, under different conditions.

Reviewed by eLife

Short disordered termini and proline-rich domain are major regulators of UBQLN1/2/4 phase separation

1. Thuy P. Dao
2. Anitha Rajendran
3. Sarasi K.K. Galagedera
4. William Haws
5. Carlos A. Castañeda

Reviewed by preLights

An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non‐Excitable Cells

1. Philipp Rühl
2. Anagha G. Nair
3. Namrata Gawande
4. Sassrika N. C. W. Dehiwalage
5. Lukas Münster
6. Roland Schönherr
7. Stefan H. Heinemann

Reviewed by Arcadia Science

Autoinhibited kinesin-1 adopts a hierarchical folding pattern

1. Zhenyu Tan
2. Yang Yue
3. Felipe Leprevost
4. Sarah Haynes
5. Venkatesha Basrur
6. Alexey I Nesvizhskii
7. Kristen J Verhey
8. Michael A Cianfrocco

• Curated by eLife

  eLife assessment

  This paper will be of significant interest to the research community working on cytoplasmic transport and microtubule motors, offering important insights into the structural arrangement of autoinhibited Kinesin-1. The paper reports a structural model of full-length kinesin-1 describing its autoinhibitory mechanism using cryo-EM, Alphafold structural predictions, cross-linking, and mass spectrometry. The data are of high quality and together offer a compelling model for how Kinesin-1 is autoinhibited, indicating that auto-inhibition does not rely on the IAK motif alone but on a more extensive intramolecular interface.

Reviewed by eLife

Single-shot quantitative phase imaging with polarization differential interference contrast

1. Mark Strassberg
2. Yana Shevtsova
3. Domenick Kamel
4. Kai Wagoner-Oshima
5. Hualin Zhong
6. Min Xu

Reviewed by Arcadia Science