Biophysics

Target cell tension regulates macrophage trogocytosis

1. Caitlin E. Cornell
2. Aymeric Chorlay
3. Deepak Krishnamurthy
4. Nicholas R. Martin
5. Lucia Baldauf
6. Daniel A. Fletcher

Reviewed by preLights

Mechanical imbalance between normal and transformed cells drives epithelial homeostasis through cell competition

1. Praver Gupta
2. Sayantani Kayal
3. Nobuyuki Tanimura
4. Shilpa P Pothapragada
5. Harish K Senapati
6. Padmashree Devendran
7. Yasuyuki Fujita
8. Dapeng Bi
9. Tamal Das

• Curated by eLife

  eLife Assessment

  In this important study, the authors combine innovative experimental approaches, including direct compressibility measurements and traction force analyses, with theoretical modeling to propose that wild-type cells exert compressive forces on softer HRasV12-transformed cells, influencing competition outcomes. The data generally provide solid evidence that transformed epithelial cells exhibit higher compressibility than wild-type cells, a property linked to their compaction during mechanical cell competition. However, the study would benefit from further characterization of how compression affects the behavior of HRasV12 cells and clearer causal links between compressibility and competition outcomes.

Reviewed by eLife

Structure and function of the human mitochondrial MRS2 channel

1. Zhihui He
2. Yung-Chi Tu
3. Chen-Wei Tsai
4. Jonathan Mount
5. Jingying Zhang
6. Ming-Feng Tsai
7. Peng Yuan

• Curated by Biophysics Colab

  Evaluation Statement (14 June 2024)

  The study by He et al. explores the structure and mechanisms of the human mitochondrial RNA splicing 2 (MRS2) protein, predicted to form Mg2+-selective channels in the mitochondrial inner membrane based on homology to the CorA family of prokaryotic Mg2+ channels. The authors use an innovative biochemical strategy to express MRS2 and perform single particle reconstructions in the absence and presence of key divalent cations. High resolution reconstructions of the pentameric channel reveal binding sites for Mg2+ and Ca2+, and electrophysiological investigations suggest that MRS2 is a Ca2+-regulated, cation-selective, Mg2+-permeable channel, in contrast to the Mg2+-regulated, Mg2+-selective CorA channel. This is an important study with interesting structural and functional observations, which will motivate further investigations of a potential role for MRS2 in mitochondrial Ca2+ signaling.

  Biophysics Colab recommends this study to scientists interested in the structure, function and regulation of cation channels as well as those working on mitochondrial transport.

  Biophysics Colab has evaluated this study as one that meets the following criteria:

  - Rigorous methodology

  - Transparent reporting

  - Appropriate interpretation

  (This evaluation refers to the version of record for this work, which is linked to and has been revised from the original preprint following peer review.)

Reviewed by Biophysics Colab

Prospective Evaluation of Structure-Based Simulations Reveal Their Ability to Predict the Impact of Kinase Mutations on Inhibitor Binding

1. Sukrit Singh
2. Vytautas Gapsys
3. Matteo Aldeghi
4. David Schaller
5. Aziz M. Rangwala
6. Jessica B. White
7. Joseph P. Bluck
8. Jenke Scheen
9. William G. Glass
10. Jiaye Guo
11. Sikander Hayat
12. Bert L. de Groot
13. Andrea Volkamer
14. Clara D. Christ
15. Markus A. Seeliger
16. John D. Chodera

Reviewed by PREreview

Torsion is a dynamic regulator of DNA replication stalling and reactivation

1. Xiaomeng Jia
2. Xiang Gao
3. Shuming Zhang
4. James T. Inman
5. Yifeng Hong
6. Anupam Singh
7. Fahad Rashid
8. James M. Berger
9. Smita S. Patel
10. Michelle D. Wang

Reviewed by PREreview

Aquavert – Imaging and Microfluidics for Vertical Swimming of Microorganisms

1. Haley B. Obenshain
2. Isaias Zarate
3. Olivia Hedman-Manzano
4. Jared Goderich
5. Sungho Lee
6. Bryant A. Lopez
7. Emma Varela
8. Ga-Young Kelly Suh
9. Douglas A. Pace
10. Siavash Ahrar

Reviewed by Arcadia Science