1. TMEM16 and OSCA/TMEM63 proteins share a conserved potential to permeate ions and phospholipids

    This article has 7 authors:
    1. Augustus J Lowry
    2. Pengfei Liang
    3. Mo Song
    4. YC Serena Wan
    5. Zhen-Ming Pei
    6. Huanghe Yang
    7. Yang Zhang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study advances our understanding of the mechanisms controlling lipid flux and ion permeation in the TMEM16 and OSCA/TMEM63 family channels. The study provides compelling new evidence indicating that side chains along the TM4/6 interface play a key role in gating lipid and ion fluxes in these channels. The authors suggest that the transmembrane channel/scramblase family proteins may have originally functioned as scramblases but lost this capacity over evolution.

    Reviewed by eLife

    This article has 10 evaluationsAppears in 2 listsLatest version Latest activity
  2. HIV integrase compacts viral DNA into biphasic condensates

    This article has 14 authors:
    1. Pauline J Kolbeck
    2. Marjolein de Jager
    3. Margherita Gallano
    4. Tine Brouns
    5. Ben Bekaert
    6. Wout Frederickx
    7. Sebastian F Konrad
    8. Siska Van Belle
    9. Frauke Christ
    10. Steven De Feyter
    11. Zeger Debyser
    12. Laura Filion
    13. Jan Lipfert
    14. Willem Vanderlinden
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The manuscript by Kolbeck and co-workers is an important contribution to understanding the physical mechanism that controls a key step in the retroviral infectious cycle. The authors employ a wide range of experimental techniques, complemented with Montecarlo simulations, that result in convincing evidence of compaction of HIV DNA by the viral integrase. This manuscript would benefit from in-depth discussion and analysis of the biophysical implications of the results.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  3. Protein-Induced Membrane Strain Drives Supercomplex Formation

    This article has 4 authors:
    1. Maximilian C Pöverlein
    2. Alexander Jussupow
    3. Hyunho Kim
    4. Ville RI Kaila
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this potentially important study, the authors conducted extensive atomistic and coarse-grained simulations as well as a lattice Monte Carlo analysis to probe the driving force and functional impact of supercomplex formation in the inner mitochondrial membrane. The study highlighted the importance of membrane mechanics to the supercomplex formation and revealed differences in structural and dynamical features of the protein components upon complex formation. In its current form, the analysis is considered incomplete, especially concerning the contributions of membrane mechanics and allosteric coupling of key regions.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitivity

    This article has 9 authors:
    1. Keisuke Motone
    2. Daphne Kontogiorgos-Heintz
    3. Jasmine Wee
    4. Kyoko Kurihara
    5. Sangbeom Yang
    6. Gwendolin Roote
    7. Yishu Fang
    8. Nicolas Cardozo
    9. Jeff Nivala

    Reviewed by preLights

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  5. Wavenumber-dependent transmission of subthreshold waves on electrical synapses network model of Caenorhabditis elegans

    This article has 3 authors:
    1. Iksoo Chang
    2. Taegon Chung
    3. Sangyeol Kim
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents numerical results on a potentially useful framework for understanding the dynamics of subthreshold waves in a network of electrical synapses modeled on the connectome data of the C elegans nematode. However, the strength of the evidence presented in favor of interference effects being a major component in subthreshold wave dynamics is inadequate.

    Reviewed by eLife

    This article has 2 evaluationsAppears in 1 listLatest version Latest activity
  6. Exploring Breast Cancer-Related Biochemical Changes in Circulating Extracellular Vesicles Using Raman Spectroscopy

    This article has 14 authors:
    1. Arianna Bonizzi
    2. Lorena Signati
    3. Maria Grimaldi
    4. Marta Truffi
    5. Francesca Piccotti
    6. Stella Gagliardi
    7. Giulia Dotti
    8. Serena Mazzucchelli
    9. Sara Albasini
    10. Roberta Cazzola
    11. Debanjan Bhowmik
    12. Chandrabhas Narayana
    13. Fabio Corsi
    14. Carlo Morasso

    Reviewed by Arcadia Science

    This article has 2 evaluationsAppears in 1 listLatest version Latest activity
  7. Fluid mechanics of luminal transport in actively contracting endoplasmic reticulum

    This article has 3 authors:
    1. Pyae Hein Htet
    2. Edward Avezov
    3. Eric Lauga
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This work explores the physical principles underlying fluid flow and luminal transport within the endoplasmic reticulum. Its important contribution is to highlight the strong physical constraints imposed by viscous dissipation in nanoscopic tubular networks. In particular, the work presents convincing evidence based on theoretical analysis that commonly discussed mechanisms such as tubular contraction are unlikely to be at the origin of the observed transport velocities. As such, it will be of relevance to cell biologists and physicists interested in organelle dynamics. As this study is solely theoretical and deals with order of magnitude estimates, its main conclusions await experimental validation.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  8. Conservation of the cooling agent binding pocket within the TRPM subfamily

    This article has 4 authors:
    1. Kate Huffer
    2. Matthew CS Denley
    3. Elisabeth V Oskoui
    4. Kenton J Swartz
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this valuable study, Huffer et al posit that non-cold sensing members of the TRPM subfamily of ion channels (e.g., TRPM2, TRPM4, TRPM5) contain a binding pocket for icilin that overlaps with the one found in the cold-activated TRPM8 channel. After examining a body of TRP channel cryo-EM structures to identify the conserved site, this study presents convincing electrophysiological evidence supporting the presence of an icilin binding pocket within TRPM4. This study shows that icilin has modulatory effects on the TRPM4 channel and will be of direct interest to those working in the TRP-channel field, but it also has implications for studies of somatosensation, taste, as well as pharmacological targeting of the TRPM subfamily.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  9. Structure of scavenger receptor SCARF1 and its interaction with lipoproteins

    This article has 14 authors:
    1. Yuanyuan Wang
    2. Fan Xu
    3. Guangyi Li
    4. Chen Cheng
    5. Bowen Yu
    6. Ze Zhang
    7. Dandan Kong
    8. Fabao Chen
    9. Yali Liu
    10. Zhen Fang
    11. Longxing Cao
    12. Yang Yu
    13. Yijun Gu
    14. Yongning He
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      SCARF1 is a scavenger membrane-bound receptor that binds modified versions of lipoproteins and has a significant role in maintaining lipid homeostasis. This useful study reports the crystal structure of SCARF1 and identifies putative binding sites for modified lipoproteins. Supported by a convincing set of experimental approaches, this study advances our knowledge of how scavenger receptors clear modified lipoproteins to maintain lipid homeostasis.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  10. TRPML1 gating modulation by allosteric mutations and lipids

    This article has 4 authors:
    1. Ninghai Gan
    2. Yan Han
    3. Weizhong Zeng
    4. Youxing Jiang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Transient receptor potential mucolipin 1 (TRPML1) functions as a lysosomal ion channel whose variants are associated with lysosomal storage disorder mucolipidosis type IV. This important report describes local and global structural changes driven by binding of regulatory phospholipids and by mutations that allosterically cause gain or loss of channel function. Most of the claims related to the allosteric regulation of TRPML1 are convincingly supported by two new cryo-EM structures which are evaluated within the context of previously reported TRPML1 structures, and a proposed allosteric gating mechanism is partially supported by functional electrophysiology results.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 2 listsLatest version Latest activity
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