Bioinformatics

RiboSnake – a user-friendly, robust, reproducible, multipurpose and documentation-extensive pipeline for 16S rRNA gene microbiome analysis

1. Ann-Kathrin Dörr
2. Josefa Welling
3. Adrian Dörr
4. Jule Gosch
5. Hannah Möhlen
6. Ricarda Schmithausen
7. Jan Kehrmann
8. Folker Meyer
9. Ivana Kraiselburd

• Curated by GigaByte

  Editors Assessment:

  This new software paper presents RiboSnake, a validated, automated, reproducible analysis pipeline implemented in the popular Snakemake workflow management system for microbiome analysis. Analysing16S rRNA gene amplicon sequencing data, this uses the widely used oQIIME2 [ tool as the basis of the workflow as it offers a wide range of functionality. Users of QIIME2 can be overwhelmed by the number of options at their disposal, and this workflow provides a fully automated and fully reproducible pipeline that can be easily installed and maintained. Providing an easy-to-navigate output accessible to non bioinformatics experts, alongside sets of already validated parameters for different types of samples. Reviewers requested some clarification for testing, worked examples and documentation, and this was improved to produce a convincingly easy-to-use workflow. Hopefully opening up an already very established technique to a new group of users and assisting them with reproducible science.

  This evaluation refers to version 1 of the preprint

Reviewed by GigaByte

TooManyCellsInteractive: A visualization tool for dynamic exploration of single-cell data

1. Conor Klamann
2. Christie J Lau
3. Javier Ruiz-Ramírez
4. Gregory W Schwartz

Reviewed by GigaScience

Phyloformer: Fast, Accurate, and Versatile Phylogenetic Reconstruction with Deep Neural Networks

1. Luca Nesterenko
2. Luc Blassel
3. Philippe Veber
4. Bastien Boussau
5. Laurent Jacob

Reviewed by Arcadia Science

Identifying images in the biology literature that are problematic for people with a color-vision deficiency

1. Harlan P Stevens
2. Carly V Winegar
3. Arwen F Oakley
4. Stephen R Piccolo

• Curated by eLife

  eLife assessment

  In this important study, the authors manually assessed randomly selected images published in eLife between 2012 and 2022 to determine whether they were accessible for readers with deuteranopia, the most common form of color vision deficiency. They then developed an automated tool designed to classify figures and images as either "friendly" or "unfriendly" for people with deuteranopia. Such a tool could be used by journals or researchers to monitor the accessibility of figures and images, and the evidence for its utility was solid: it performed well for eLife articles, but performance was weaker for a broader dataset of PubMed articles, which were not included in the training data. The authors also provide code that readers can download and run to test their own images, and this may be of most use for testing the tool, as there are already several free, user-friendly recoloring programs that allow users to see how images would look to a person with different forms of color vision deficiency. Automated classifications are of most use for assessing many images, when the user does not have the time or resources to assess each image individually.

Reviewed by eLife

ImmCellTyper facilitates systematic mass cytometry data analysis for deep immune profiling

1. Jing Sun
2. Desmond Choy
3. Nicolas Sompairac
4. Shirin Jamshidi
5. Michele Mishto
6. Shahram Kordasti

• Curated by eLife

  eLife assessment

  ImmCellTyper presents a useful toolkit for CyTOF data analysis, integrating BinaryClust for semi-supervised clustering and cell type annotation. The evidence supporting the findings is convincing, with appropriate and validated methodology. This tool will be helpful to researchers in immunology and cytometry, offering a robust solution for cell type identification and differential analysis.

Reviewed by eLife

MOBFinder: a tool for MOB typing for plasmid metagenomic fragments based on language model

1. Tao Feng
2. Shufang Wu
3. Hongwei Zhou
4. Zhencheng Fang

Reviewed by GigaScience

Deciphering cancer genomes with GenomeSpy: a grammar-based visualization toolkit

1. Kari Lavikka
2. Jaana Oikkonen
3. Yilin Li
4. Taru Muranen
5. Giulia Micoli
6. Giovanni Marchi
7. Alexandra Lahtinen
8. Kaisa Huhtinen
9. Rainer Lehtonen
10. Sakari Hietanen
11. Johanna Hynninen
12. Anni Virtanen
13. Sampsa Hautaniemi

Reviewed by GigaScience

Impact of reference design on estimating SARS-CoV-2 lineage abundances from wastewater sequencing data

1. Eva Aßmann
2. Shelesh Agrawal
3. Laura Orschler
4. Sindy Böttcher
5. Susanne Lackner
6. Martin Hölzer

Reviewed by GigaScience

Peptipedia v2.0: A peptide sequence database and user-friendly web platform. A major update

1. Gabriel Cabas-Mora
2. Anamaría Daza
3. Nicole Soto-García
4. Valentina Garrido
5. Diego Alvarez
6. Marcelo Navarrete
7. Lindybeth Sarmiento-Varón
8. Julieta H. Sepúlveda Yañez
9. Mehdi D. Davari
10. Frederic Cadet
11. Álvaro Olivera-Nappa
12. Roberto Uribe-Paredes
13. David Medina-Ortiz

Reviewed by Arcadia Science

Multiple Protein Structure Alignment at Scale with FoldMason

1. Cameron L.M. Gilchrist
2. Milot Mirdita
3. Martin Steinegger

Reviewed by Arcadia Science