Bioengineering

Single domain shark VNAR antibodies neutralize SARS‐CoV‐2 infection in vitro

1. Aziz Gauhar
2. Cyril V. Privezentzev
3. Mykhaylo Demydchuk
4. Tanja Gerlza
5. Julia Rieger
6. Andreas J. Kungl
7. Frank S. Walsh
8. J. Lynn Rutkowski
9. Pawel Stocki

Reviewed by ScreenIT

Human inhalable antibody fragments neutralizing SARS-CoV-2 variants for COVID-19 therapy

1. Olga Minenkova
2. Daniela Santapaola
3. Ferdinando Maria Milazzo
4. Anna Maria Anastasi
5. Gianfranco Battistuzzi
6. Caterina Chiapparino
7. Antonio Rosi
8. Giuseppe Gritti
9. Gianmaria Borleri
10. Alessandro Rambaldi
11. Clélia Dental
12. Cécile Viollet
13. Bruno Pagano
14. Laura Salvini
15. Emanuele Marra
16. Laura Luberto
17. Antonio Rossi
18. Anna Riccio
19. Emilio Merlo Pich
20. Maria Gabriella Santoro
21. Rita De Santis

Reviewed by ScreenIT

Sub-Picomolar Detection of SARS-CoV-2 RBD via Computationally-Optimized Peptide Beacons

1. Soumya P. Tripathy
2. Manvitha Ponnapati
3. Joseph Jacobson
4. Pranam Chatterjee

Reviewed by ScreenIT

Enteric Coronavirus Infection and Treatment Modeled With an Immunocompetent Human Intestine-On-A-Chip

1. Amir Bein
2. Seongmin Kim
3. Girija Goyal
4. Wuji Cao
5. Cicely Fadel
6. Arash Naziripour
7. Sanjay Sharma
8. Ben Swenor
9. Nina LoGrande
10. Atiq Nurani
11. Vincent N. Miao
12. Andrew W. Navia
13. Carly G. K. Ziegler
14. José Ordovas Montañes
15. Pranav Prabhala
16. Min Sun Kim
17. Rachelle Prantil-Baun
18. Melissa Rodas
19. Amanda Jiang
20. Lucy O’Sullivan
21. Gladness Tillya
22. Alex K. Shalek
23. Donald E. Ingber

Reviewed by ScreenIT

An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens

1. Robert Coukos
2. David Yao
3. Mateo I Sanchez
4. Eric T Strand
5. Meagan E Olive
6. Namrata D Udeshi
7. Jonathan S Weissman
8. Steven A Carr
9. Michael C Bassik
10. Alice Y Ting

• Curated by eLife

  Evaluation Summary:

  Here authors present a robust, high-throughput and genome-wide strategy to identify genes that influence protein localization in individual subcellular compartments. The results profile subsets of genes that are involved in localization of tail-anchored proteins to mitochondrial and endoplasmic reticulum membranes and identify several unexpected regulators. This new tool is adaptable to studying various types of protein trafficking processes to shed light on their molecular mechanisms.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife, preLights

Humans optimally anticipate and compensate for an uneven step during walking

1. Osman Darici
2. Arthur D Kuo

• Curated by eLife

  Evaluation Summary:

  This study provides experimental evidence supporting an energy optimality principle for walking over uneven terrain. Using a simple rimless wheel model of human walking, the authors previously predicted speed fluctuations to emerge when a step up or down when energy is minimized over the entire walking path. New experimental evidence provides evidence that both anticipatory and reactive adjustments used by the nervous system follow the predictions of an energy minimization principle. The predictive power of an energy-minimization principle during transient, non-steady state behavior is notable. Certain issues regarding the generalizability of the results to variable step lengths and timing, alternative optimality criteria, and limitations of the modeling assumptions should be clarified.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife, preLights

Antibody Display of cell surface receptor Tetraspanin12 and SARS-CoV-2 spike protein

1. Fu-Lien Hsieh
2. Tao-Hsin Chang

Reviewed by ScreenIT

Broadening a SARS-CoV-1–neutralizing antibody for potent SARS-CoV-2 neutralization through directed evolution

1. Fangzhu Zhao
2. Meng Yuan
3. Celina Keating
4. Namir Shaabani
5. Oliver Limbo
6. Collin Joyce
7. Jordan Woehl
8. Shawn Barman
9. Alison Burns
10. Quoc Tran
11. Xueyong Zhu
12. Michael Ricciardi
13. Linghang Peng
14. Jessica Smith
15. Deli Huang
16. Bryan Briney
17. Devin Sok
18. David Nemazee
19. John R. Teijaro
20. Ian A. Wilson
21. Dennis R. Burton
22. Joseph G. Jardine

Reviewed by ScreenIT

Generation of potent cellular and humoral immunity against SARS-CoV-2 antigens via conjugation to a polymeric glyco-adjuvant

1. Laura T. Gray
2. Michal M. Raczy
3. Priscilla S. Briquez
4. Tiffany M. Marchell
5. Aaron T. Alpar
6. Rachel P. Wallace
7. Lisa R. Volpatti
8. Maria Stella Sasso
9. Shijie Cao
10. Mindy Nguyen
11. Aslan Mansurov
12. Erica Budina
13. Elyse A. Watkins
14. Ani Solanki
15. Nikolaos Mitrousis
16. Joseph W. Reda
17. Shann S. Yu
18. Andrew C. Tremain
19. Ruyi Wang
20. Vlad Nicolaescu
21. Kevin Furlong
22. Steve Dvorkin
23. Balaji Manicassamy
24. Glenn Randall
25. D. Scott Wilson
26. Marcin Kwissa
27. Melody A. Swartz
28. Jeffrey A. Hubbell

Reviewed by ScreenIT

High level production and characterization of truncated human angiotensin converting enzyme 2 in Nicotiana benthamiana plant as a potential therapeutic target in COVID-19

1. Tarlan Mamedov
2. İrem Gürbüzaslan
3. Merve Ilgin
4. Damla Yuksel
5. Gunay Mammadova
6. Aykut Ozkul
7. Gulnara Hasanova

Reviewed by ScreenIT