Biochemistry

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp3 papain-like protease

1. Chew Theng Lim
2. Kang Wei Tan
3. Mary Wu
4. Rachel Ulferts
5. Lee A. Armstrong
6. Eiko Ozono
7. Lucy S. Drury
8. Jennifer C. Milligan
9. Theresa U. Zeisner
10. Jingkun Zeng
11. Florian Weissmann
12. Berta Canal
13. Ganka Bineva-Todd
14. Michael Howell
15. Nicola O'Reilly
16. Rupert Beale
17. Yogesh Kulathu
18. Karim Labib
19. John F.X. Diffley

Reviewed by ScreenIT

Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escape

1. Fred D Mast
2. Peter C Fridy
3. Natalia E Ketaren
4. Junjie Wang
5. Erica Y Jacobs
6. Jean Paul Olivier
7. Tanmoy Sanyal
8. Kelly R Molloy
9. Fabian Schmidt
10. Magdalena Rutkowska
11. Yiska Weisblum
12. Lucille M Rich
13. Elizabeth R Vanderwall
14. Nicholas Dambrauskas
15. Vladimir Vigdorovich
16. Sarah Keegan
17. Jacob B Jiler
18. Milana E Stein
19. Paul Dominic B Olinares
20. Louis Herlands
21. Theodora Hatziioannou
22. D Noah Sather
23. Jason S Debley
24. David Fenyö
25. Andrej Sali
26. Paul D Bieniasz
27. John D Aitchison
28. Brian T Chait
29. Michael P Rout

• Curated by eLife

  Evaluation Summary:

  The manuscript by Mast et al. describes an impressive collection of new nanobodies against SARS-CoV-2 spike, which probably provides the most complete coverage of the accessible epitopes of spike to date. The authors thoroughly characterize biophysical and functional properties of the nanobodies and set an example of how to best combine multiple nanobodies to target a pathogen. As the latest in a long series of SARS-CoV-2 nanobody papers, this study stands out for its completeness, although it does not provide a novel mechanism of action or biological insights.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT

A High-Throughput Radioactivity-Based Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex

1. Aliakbar Khalili Yazdi
2. Fengling Li
3. Kanchan Devkota
4. Sumera Perveen
5. Pegah Ghiabi
6. Taraneh Hajian
7. Albina Bolotokova
8. Masoud Vedadi

Reviewed by ScreenIT

Conserved cardiolipin-mitochondrial ADP/ATP carrier interactions assume distinct structural and functional roles that are clinically relevant

1. Nanami Senoo
2. Dinesh K. Chinthapalli
3. Matthew G. Baile
4. Vinaya K. Golla
5. Bodhisattwa Saha
6. Oluwaseun B. Ogunbona
7. James A. Saba
8. Teona Munteanu
9. Yllka Valdez
10. Kevin Whited
11. Dror Chorev
12. Nathan N. Alder
13. Eric R. May
14. Carol V. Robinson
15. Steven M. Claypool

Reviewed by preLights

A high-throughput fluorescence polarization assay to discover inhibitors of arenavirus and coronavirus exoribonucleases

1. Sergio Hernández
2. Mikael Feracci
3. Carolina Trajano De Jesus
4. Priscila El-Kazzi
5. Rafik Kaci
6. Laura Garlatti
7. Etienne Decroly
8. Bruno Canard
9. François Ferron
10. Karine Alvarez

Reviewed by ScreenIT

Purification of recombinant SARS-CoV-2 spike, its receptor binding domain, and CR3022 mAb for serological assay

1. Kang Lan Tee
2. Philip J. Jackson
3. Joseph M. Scarrott
4. Stephen R. P. Jaffe
5. Abayomi O. Johnson
6. Yusuf Johari
7. Thilo H. Pohle
8. Theo Mozzanino
9. Joseph Price
10. James Grinham
11. Adam Brown
12. Martin J. Nicklin
13. David C. James
14. Mark J. Dickman
15. Tuck Seng Wong

Reviewed by ScreenIT

Identification of a conserved S2 epitope present on spike proteins from all highly pathogenic coronaviruses

1. Rui P Silva
2. Yimin Huang
3. Annalee W Nguyen
4. Ching-Lin Hsieh
5. Oladimeji S Olaluwoye
6. Tamer S Kaoud
7. Rebecca E Wilen
8. Ahlam N Qerqez
9. Jun-Gyu Park
10. Ahmed M Khalil
11. Laura R Azouz
12. Kevin C Le
13. Amanda L Bohanon
14. Andrea M DiVenere
15. Yutong Liu
16. Alison G Lee
17. Dzifa A Amengor
18. Sophie R Shoemaker
19. Shawn M Costello
20. Eduardo A Padlan
21. Susan Marqusee
22. Luis Martinez-Sobrido
23. Kevin N Dalby
24. Sheena D'Arcy
25. Jason S McLellan
26. Jennifer A Maynard

• Curated by eLife

  eLife assessment

  Silva and colleagues present useful findings related to the isolation of an anti-S2 antibody that recognizes a previously uncharacterized SARS-CoV2 Spike (S) epitope, adding to the growing repertoire of anti-S antibodies that broadly cross-react against human and zoonotic coronaviruses. The evidence supporting the claims of the authors is solid, although antibody effectiveness as a prophylactic or therapeutic reagent in an animal model would have strengthened the study. The work will be of interest to biologists working to develop pan-coronavirus therapies.

Reviewed by eLife, ScreenIT

An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

1. Michael Schoof
2. Bryan Faust
3. Reuben A. Saunders
4. Smriti Sangwan
5. Veronica Rezelj
6. Nick Hoppe
7. Morgane Boone
8. Christian B. Billesbølle
9. Cristina Puchades
10. Caleigh M. Azumaya
11. Huong T. Kratochvil
12. Marcell Zimanyi
13. Ishan Deshpande
14. Jiahao Liang
15. Sasha Dickinson
16. Henry C. Nguyen
17. Cynthia M. Chio
18. Gregory E. Merz
19. Michael C. Thompson
20. Devan Diwanji
21. Kaitlin Schaefer
22. Aditya A. Anand
23. Niv Dobzinski
24. Beth Shoshana Zha
25. Camille R. Simoneau
26. Kristoffer Leon
27. Kris M. White
28. Un Seng Chio
29. Meghna Gupta
30. Mingliang Jin
31. Fei Li
32. Yanxin Liu
33. Kaihua Zhang
34. David Bulkley
35. Ming Sun
36. Amber M. Smith
37. Alexandrea N. Rizo
38. Frank Moss
39. Axel F. Brilot
40. Sergei Pourmal
41. Raphael Trenker
42. Thomas Pospiech
43. Sayan Gupta
44. Benjamin Barsi-Rhyne
45. Vladislav Belyy
46. Andrew W. Barile-Hill
47. Silke Nock
48. Yuwei Liu
49. Nevan J. Krogan
50. Corie Y. Ralston
51. Danielle L. Swaney
52. Adolfo García-Sastre
53. Melanie Ott
54. Marco Vignuzzi
55. QCRG Structural Biology Consortium
56. Peter Walter
57. Aashish Manglik
58. Caleigh M. Azumaya
59. Cristina Puchades
60. Ming Sun
61. Julian R. Braxton
62. Axel F. Brilot
63. Meghna Gupta
64. Fei Li
65. Kyle E. Lopez
66. Arthur Melo
67. Gregory E. Merz
68. Frank Moss
69. Joana Paulino
70. Thomas H. Pospiech
71. Sergei Pourmal
72. Alexandrea N. Rizo
73. Amber M. Smith
74. Paul V. Thomas
75. Feng Wang
76. Zanlin Yu
77. Miles Sasha Dickinson
78. Henry C. Nguyen
79. Daniel Asarnow
80. Melody G. Campbell
81. Cynthia M. Chio
82. Un Seng Chio
83. Devan Diwanji
84. Bryan Faust
85. Meghna Gupta
86. Nick Hoppe
87. Mingliang Jin
88. Junrui Li
89. Yanxin Liu
90. Gregory E. Merz
91. Smriti Sangwan
92. Tsz Kin Martin Tsui
93. Raphael Trenker
94. Donovan Trinidad
95. Eric Tse
96. Kaihua Zhang
97. Fengbo Zhou
98. Nadia Herrera
99. Huong T. Kratochvil
100. Ursula Schulze-Gahmen
101. Michael C. Thompson
102. Iris D. Young
103. Justin Biel
104. Ishan Deshpande
105. Xi Liu
106. Christian Bache Billesbølle
107. Carlos Nowotny
108. Amber M. Smith
109. Jianhua Zhao
110. Alisa Bowen
111. Nick Hoppe
112. Yen-Li Li
113. Phuong Nguyen
114. Mali Safari
115. Kaitlin Schaefer
116. Natalie Whitis
117. Michelle Moritz
118. Tristan W. Owens
119. Amy Diallo
120. Kate Kim
121. Jessica K. Peters
122. Erron W. Titus
123. Jenny Chen
124. Loan Doan
125. Sebastian Flores
126. Victor L. Lam
127. Yang Li
128. Megan Lo
129. Aye C. Thwin
130. Stephanie Wankowicz
131. Yang Zhang
132. David Bulkley
133. Arceli Joves
134. Almarie Joves
135. Liam McKay
136. Mariano Tabios
137. Oren S. Rosenberg
138. Kliment A. Verba
139. David A. Agard
140. Yifan Cheng
141. James S. Fraser
142. Adam Frost
143. Natalia Jura
144. Tanja Kortemme
145. Nevan J. Krogan
146. Aashish Manglik
147. Daniel R. Southworth
148. Robert M. Stroud

Reviewed by ScreenIT

Inhibition of amyloid formation of the Nucleoprotein of SARS-CoV-2

1. Einav Tayeb-Fligelman
2. Xinyi Cheng
3. Christen Tai
4. Jeannette T. Bowler
5. Sarah Griner
6. Michael R. Sawaya
7. Paul M. Seidler
8. Yi Xiao Jiang
9. Jiahui Lu
10. Gregory M. Rosenberg
11. Lukasz Salwinski
12. Romany Abskharon
13. Chih-Te Zee
14. Ke Hou
15. Yan Li
16. David R. Boyer
17. Kevin A. Murray
18. Genesis Falcon
19. Daniel H. Anderson
20. Duilio Cascio
21. Lorena Saelices
22. Robert Damoiseaux
23. Feng Guo
24. David S. Eisenberg

Reviewed by ScreenIT

SARS-CoV-2 ORF7b: is a bat virus protein homologue a major cause of COVID-19 symptoms?

1. Marie-Laure Fogeron
2. Roland Montserret
3. Johannes Zehnder
4. Minh-Ha Nguyen
5. Marie Dujardin
6. Louis Brigandat
7. Laura Cole
8. Marti Ninot-Pedrosa
9. Lauriane Lecoq
10. Beat H Meier
11. Anja Böckmann

Reviewed by ScreenIT