A robust high-throughput fluorescent polarization assay for the evaluation and screening of SARS-CoV-2 fusion inhibitors
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SciScore for 10.1101/2021.06.17.448891: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources Ltd (Guangzhou, China) and inserted into plasmid pET-44b(+) and pET-28a(+), respectively. pET-44b(+suggested: NonepET-28a(+)suggested: RRID:Addgene_108949)2.2 Protein expression and purification: The plasmids pET-44b-NusA-5HB, pET-28a-5HB, and pCold-NusA containing the gene encoding NusA-5HB, 5HB and NusA (with N-terminal His-tag) were transformed into competent E. coli BL21 (DE3) cells (Invitrogen) respectively. pET-28a-5HBsuggested: NonepCold-NusAsuggested: NoneThe recombinant E. coli was cultivated in LB medium … SciScore for 10.1101/2021.06.17.448891: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources Ltd (Guangzhou, China) and inserted into plasmid pET-44b(+) and pET-28a(+), respectively. pET-44b(+suggested: NonepET-28a(+)suggested: RRID:Addgene_108949)2.2 Protein expression and purification: The plasmids pET-44b-NusA-5HB, pET-28a-5HB, and pCold-NusA containing the gene encoding NusA-5HB, 5HB and NusA (with N-terminal His-tag) were transformed into competent E. coli BL21 (DE3) cells (Invitrogen) respectively. pET-28a-5HBsuggested: NonepCold-NusAsuggested: NoneThe recombinant E. coli was cultivated in LB medium with antibiotic (50 mg/L Kan for pET-28a-5HB; 100 mg/L Amp for pET-44b-NusA-5HB and pCold-NusA) at 37 °C and rotary shaking at 200 rpm. pET-44b-NusA-5HBsuggested: NoneSoftware and Algorithms Sentences Resources The obtained f values were plotted using GraphPad Prism 8 to calculate the IC50. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)PyMOL (Version 1.5, Schrö dinger, LLC) was used to prepare figures of the complexes. PyMOLsuggested: (PyMOL, RRID:SCR_000305)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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