Biochemistry

Allotypic variation in antigen processing controls antigenic peptide generation from SARS-CoV-2 S1 spike glycoprotein

1. George Stamatakis
2. Martina Samiotaki
3. Ioannis Temponeras
4. George Panayotou
5. Efstratios Stratikos

Reviewed by ScreenIT

Implications of Spike-glycoprotein processing at S1/S2 by Furin, at S2’ by Furin and/or TMPRSS2 and shedding of ACE2: cell-to-cell fusion, cell entry and infectivity of SARS-CoV-2

1. Rachid Essalmani
2. Jaspreet Jain
3. Delia Susan-Resiga
4. Ursula Andréo
5. Alexandra Evagelidis
6. Rabeb Mouna Derbali
7. David N. Huynh
8. Frédéric Dallaire
9. Mélanie Laporte
10. Adrien Delpal
11. Priscila Sutto-Ortiz
12. Bruno Coutard
13. Claudine Mapa
14. Keith Wilcoxen
15. Étienne Decroly
16. Tram NQ Pham
17. Éric A. Cohen
18. Nabil G. Seidah

Reviewed by ScreenIT

Flavonols and dihydroflavonols inhibit the main protease activity of SARS-CoV-2 and the replication of human coronavirus 229E

1. Yue Zhu
2. Frank Scholle
3. Samantha C. Kisthardt
4. De-Yu Xie

Reviewed by ScreenIT

Epitope order Matters in multi-epitope-based peptide (MEBP) vaccine design: An in silico study

1. Muthu Raj Salaikumaran
2. Prasanna Sudharson Kasamuthu
3. V L S Prasad Burra

Reviewed by ScreenIT

The challenge of structural heterogeneity in the native mass spectrometry studies of the SARS-CoV-2 spike protein interactions with its host cell-surface receptor

1. Yang Yang
2. Daniil G. Ivanov
3. Igor A. Kaltashov

Reviewed by ScreenIT

Structure and activity of human TMPRSS2 protease implicated in SARS-CoV-2 activation

1. Bryan J. Fraser
2. Serap Beldar
3. Almagul Seitova
4. Ashley Hutchinson
5. Dhiraj Mannar
6. Yanjun Li
7. Daniel Kwon
8. Ruiyan Tan
9. Ryan P. Wilson
10. Karoline Leopold
11. Sriram Subramaniam
12. Levon Halabelian
13. Cheryl H. Arrowsmith
14. François Bénard

Reviewed by ScreenIT

SARS-CoV-2 activates ER stress and Unfolded protein response

1. Livia Rosa-Fernandes
2. Lucas C. Lazari
3. Janaina Macedo da Silva
4. Vinicius de Morais Gomes
5. Rafael Rahal Guaragna Machado
6. Ancely Ferreira dos Santos
7. Danielle Bastos Araujo
8. João Vitor Paccini Coutinho
9. Gabriel Santos Arini
10. Claudia B. Angeli
11. Edmarcia E. de Souza
12. Carsten Wrenger
13. Claudio R. F. Marinho
14. Danielle B. L. Oliveira
15. Edison L. Durigon
16. Leticia Labriola
17. Giuseppe Palmisano

Reviewed by ScreenIT

A robust high-throughput fluorescent polarization assay for the evaluation and screening of SARS-CoV-2 fusion inhibitors

1. Xinjian Yin
2. Litong Chen
3. Siwen Yuan
4. Lan Liu
5. Zhizeng Gao

Reviewed by ScreenIT

Comparative structural analyses of selected spike protein-RBD mutations in SARS-CoV-2 lineages

1. Urmi Roy

Reviewed by ScreenIT

Crosstalk between repair pathways elicits double-strand breaks in alkylated DNA and implications for the action of temozolomide

1. Robert P Fuchs
2. Asako Isogawa
3. Joao A Paulo
4. Kazumitsu Onizuka
5. Tatsuro Takahashi
6. Ravindra Amunugama
7. Julien P Duxin
8. Shingo Fujii

• Curated by eLife

  Evaluation summary:

  Glioblastomas, like many tumors, consist of a cohort of actively dividing cells and a substantially larger fraction of non-proliferating cells. The standard of care involves the administration of a chemotherapy drug (temozolomide (TMZ)) whose antitumor activity is thought to be dependent on a toxic intermediate produced during DNA replication. In this report, the authors show how this compound is also processed by the interaction of two DNA repair pathways which produce the same intermediate without the requirement for DNA replication. The paper will be of interest to those scientists concerned with the implications of DNA damage and repair for cancer chemotherapy, particularly for tumors as deadly as glioblastoma.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife