Biochemistry

Structural and biochemical rationale for enhanced spike protein fitness in delta and kappa SARS-CoV-2 variants

1. James W. Saville
2. Dhiraj Mannar
3. Xing Zhu
4. Shanti S. Srivastava
5. Alison M. Berezuk
6. Jean-Philippe Demers
7. Steven Zhou
8. Katharine S. Tuttle
9. Inna Sekirov
10. Andrew Kim
11. Wei Li
12. Dimiter S. Dimitrov
13. Sriram Subramaniam

Reviewed by ScreenIT

Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptor

1. Tümay Capraz
2. Nikolaus F Kienzl
3. Elisabeth Laurent
4. Jan W Perthold
5. Esther Föderl-Höbenreich
6. Clemens Grünwald-Gruber
7. Daniel Maresch
8. Vanessa Monteil
9. Janine Niederhöfer
10. Gerald Wirnsberger
11. Ali Mirazimi
12. Kurt Zatloukal
13. Lukas Mach
14. Josef M Penninger
15. Chris Oostenbrink
16. Johannes Stadlmann

• Curated by eLife

  Evaluation Summary:

  This manuscript is of broad interest in light of the COVID-19 pandemic. Characterization of how glycosylation affects interactions between the viral Spike protein and ACE2 receptor can inform efforts to inhibit the SARS-CoV-2. The molecular modeling and functional analysis need to be improved.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife, ScreenIT

Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid and Potential Utility for SARS-CoV-2 Detection

1. Xiaoyu Zhang
2. Venubabu Kotikam
3. Eriks Rozners
4. Brian P. Callahan

Reviewed by ScreenIT

Colloidal Aggregators in Biochemical SARS-CoV-2 Repurposing Screens

1. Henry R. O’Donnell
2. Tia A. Tummino
3. Conner Bardine
4. Charles S. Craik
5. Brian K. Shoichet

Reviewed by ScreenIT

Validation and invalidation of SARS-CoV-2 main protease inhibitors using the Flip-GFP and Protease-Glo luciferase assays

1. Chunlong Ma
2. Haozhou Tan
3. Juliana Choza
4. Yuyin Wang
5. Jun Wang

Reviewed by ScreenIT

Endothelial pannexin 1–TRPV4 channel signaling lowers pulmonary arterial pressure in mice

1. Zdravka Daneva
2. Matteo Ottolini
3. Yen Lin Chen
4. Eliska Klimentova
5. Maniselvan Kuppusamy
6. Soham A Shah
7. Richard D Minshall
8. Cheikh I Seye
9. Victor E Laubach
10. Brant E Isakson
11. Swapnil K Sonkusare

• Curated by eLife

  Evaluation Summary:

  This study, which makes a connection between several proteins known to regulate endothelial function in pulmonary arteries, may be of interest to vascular, pulmonary and ion channel physiologists. The study provides compelling evidence that ATP released from pulmonary artery endothelial cell (EC) pannexin1 channels activates TRPV4 channels via an EC P2Y2R-PLC-DAG-PKC alpha pathway that is facilitated by the scaffolding protein Caveolin-1 and that this pathway helps to maintain low pulmonary vascular resistance and pulmonary artery pressure. Identification of this pathway provides new drug targets to improve pulmonary endothelial function in disease states such characterized by impaired endothelial function. What remains to be established or understood is the physiological stimulus for activation of the pannexin1 channels and ATP release and also the potential dark-side of overactivity of EC TRPV4 channels, which appear to have negative effects on EC function.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Structural remodeling of SARS-CoV-2 spike protein glycans reveals the regulatory roles in receptor-binding affinity

1. Yen-Pang Hsu
2. Martin Frank
3. Debopreeti Mukherjee
4. Vladimir Shchurik
5. Alexey Makarov
6. Benjamin F Mann

Reviewed by ScreenIT

Structural analysis of receptor binding domain mutations in SARS-CoV-2 variants of concern that modulate ACE2 and antibody binding

1. Dhiraj Mannar
2. James W. Saville
3. Xing Zhu
4. Shanti S. Srivastava
5. Alison M. Berezuk
6. Steven Zhou
7. Katharine S. Tuttle
8. Andrew Kim
9. Wei Li
10. Dimiter S. Dimitrov
11. Sriram Subramaniam

Reviewed by ScreenIT

Antiviral cyclic peptides targeting the main protease of SARS-CoV-2

1. Jason Johansen-Leete
2. Sven Ullrich
3. Sarah E. Fry
4. Rebecca Frkic
5. Max J. Bedding
6. Anupriya Aggarwal
7. Anneliese S. Ashhurst
8. Kasuni B. Ekanayake
9. Mithun C. Mahawaththa
10. Vishnu M. Sasi
11. Stephanie Luedtke
12. Daniel J. Ford
13. Anthony J. O'Donoghue
14. Toby Passioura
15. Mark Larance
16. Gottfried Otting
17. Stuart Turville
18. Colin J. Jackson
19. Christoph Nitsche
20. Richard J. Payne

Reviewed by ScreenIT

A universal pocket in fatty acyl-AMP ligases ensures redirection of fatty acid pool away from coenzyme A-based activation

1. Gajanan S Patil
2. Priyadarshan Kinatukara
3. Sudipta Mondal
4. Sakshi Shambhavi
5. Ketan D Patel
6. Surabhi Pramanik
7. Noopur Dubey
8. Subhash Narasimhan
9. Murali Krishna Madduri
10. Biswajit Pal
11. Rajesh S Gokhale
12. Rajan Sankaranarayanan

• Curated by eLife

  Evaluation Summary:

  This study addresses the structural basis of the ability of fatty acyl-AMP ligases (FAAL) to exclude condensation of activated fatty acids with coenzyme-A and facilitate the reaction with other 4-phosphopantetheine linked acceptors. This issue is of significant interest with regard to understanding how certain fatty acids are channeled to specific metabolic fates. The structural question at hand is the apparent discrimination of the CoA moiety (adenosine 3',5'-bisphosphate) versus a holo-ACP tethered to the 4-phosphopantethein head group. This work will contribute significantly to our current knowledge of how distinct classes of enzymes divert fatty acids to virulent lipids in mycobacteria, and it will be more broadly of interest for metabolic engineering.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife