Biochemistry

Rate-limiting transport of positively charged arginine residues through the Sec-machinery is integral to the mechanism of protein secretion

1. William J Allen
2. Robin A Corey
3. Daniel W Watkins
4. A Sofia F Oliveira
5. Kiel Hards
6. Gregory M Cook
7. Ian Collinson

• Curated by eLife

  Evaluation Summary:

  Here, using a novel bioluminescence-based assay, authors dissect the sequence features of client proteins that influence SecA/SecYEG-mediated protein translocation across the bacterial inner membranes. Combined with rigorous kinetic modeling, this study pushes the description of this important cellular pathway towards a highly detailed level, which will potentially advance our understanding of ATP-driven protein secretion mechanisms in bacteria. The main conclusions are well supported, and the paper will be interesting to both those in the field of protein transport and to a broader audience.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

A network of cytosolic (co)chaperones promotes the biogenesis of mitochondrial signal-anchored outer membrane proteins

1. Layla Drwesh
2. Benjamin Heim
3. Max Graf
4. Linda Kehr
5. Lea Hansen-Palmus
6. Mirita Franz-Wachtel
7. Boris Macek
8. Hubert Kalbacher
9. Johannes Buchner
10. Doron Rapaport

• Curated by eLife

  Evaluation Summary:

  The authors dissect and reconstitute the cytosolic steps for mitochondrial signal-anchored membrane protein biogenesis focusing on post-translational precursor recognition by cytosolic chaperones and their subsequent transfer to import receptors located within the mitochondrial outer membrane. These are crucial events in order to assist proper protein biogenesis while preventing aggregation and its downstream consequences. The study is an important contribution to the understanding of cytosolic events in the biogenesis of mitochondrial proteins, and this paper will be of relevance for researchers in the fields of chaperone and mitochondrial biology as well as for cell biologists studying the biogenesis of membrane proteins.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Changes of urinary proteomic before and after QIV and COVID-19 vaccination

1. Xuanzhen Pan
2. Yongtao Liu
3. Yijin Bao
4. Lilong Wei
5. Youhe Gao

Reviewed by ScreenIT

Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-Like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system

1. Carolina De Marco Verissimo
2. Jesus Lopez-Corrales
3. Amber L. Dorey
4. Krystyna Cwiklinski
5. Richard Lalor
6. Nichola Eliza Davies Calvani
7. Heather L. Jewhurst
8. Sean Doyle
9. John P. Dalton

Reviewed by ScreenIT

LION/web: a web-based ontology enrichment tool for lipidomic data analysis

1. Martijn R Molenaar
2. Aike Jeucken
3. Tsjerk A Wassenaar
4. Chris H A van de Lest
5. Jos F Brouwers
6. J Bernd Helms

Reviewed by GigaScience

Cytokine and chemokine profile in patients hospitalized with COVID-19: A comparative study

1. Abdisa Tufa
2. Tewodros Haile Gebremariam
3. Tsegahun Manyazewal
4. Tewodros Getinet
5. Dominic-Luc Webb
6. Per M. Hellström
7. Solomon Genet

Reviewed by ScreenIT

Targeting an evolutionarily conserved “E-L-L” motif in spike protein to identify a small molecule fusion inhibitor against SARS-CoV-2

1. Indrani Das Jana
2. Prabuddha Bhattacharya
3. Karthick Mayilsamy
4. Saptarshi Banerjee
5. Gourab Bhattacharje
6. Sayan Das
7. Seemanti Aditya
8. Anandita Ghosh
9. Andrew R McGill
10. Syamanthak Srikrishnan
11. Amit Kumar Das
12. Amit Basak
13. Shyam S Mohapatra
14. Bala Chandran
15. Devesh Bhimsaria
16. Subhra Mohapatra
17. Arunava Roy
18. Arindam Mondal

Reviewed by ScreenIT

Molecular docking between human TMPRSS2 and the serine protease Kunitz-type inhibitor rBmTI-A

1. Lívia de Moraes Bomediano Camillo
2. Sergio Daishi Sasaki

Reviewed by ScreenIT

Doublecortin engages the microtubule lattice through a cooperative binding mode involving its C-terminal domain

1. Atefeh Rafiei
2. Sofía Cruz Tetlalmatzi
3. Claire H Edrington
4. Linda Lee
5. D Alex Crowder
6. Daniel J Saltzberg
7. Andrej Sali
8. Gary Brouhard
9. David C Schriemer

• Curated by eLife

  Evaluation Summary:

  Rafiei et al. investigate the molecular architecture of an important neuronal microtubule-associated protein, doublecortin, bound to the microtubule by integrating data from chemical cross-linking experiments with available crystallographic and cryo-EM structures. They present an appealing model of microtubule-mediated self-association of doublecortin; however, they do not perform any additional assays to support the functional relevance of this model. In addition, there are limitations to the used method in resolving structural details. The manuscript will be relevant for biologists with an interest in microtubule formation and to researchers who apply different structural biology tools to study the organization of large biomolecular assemblies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Crystal structures and fragment screening of SARS-CoV-2 NSP14 reveal details of exoribonuclease activation and mRNA capping and provide starting points for antiviral drug development

1. Nergis Imprachim
2. Yuliana Yosaatmadja
3. Joseph A Newman

Reviewed by ScreenIT