1. Structural diversity of the SARS-CoV-2 Omicron spike

    This article has 25 authors:
    1. Sophie M.-C. Gobeil
    2. Rory Henderson
    3. Victoria Stalls
    4. Katarzyna Janowska
    5. Xiao Huang
    6. Aaron May
    7. Micah Speakman
    8. Esther Beaudoin
    9. Kartik Manne
    10. Dapeng Li
    11. Rob Parks
    12. Maggie Barr
    13. Margaret Deyton
    14. Mitchell Martin
    15. Katayoun Mansouri
    16. Robert J. Edwards
    17. Amanda Eaton
    18. David C. Montefiori
    19. Gregory D. Sempowski
    20. Kevin O. Saunders
    21. Kevin Wiehe
    22. Wilton Williams
    23. Bette Korber
    24. Barton F. Haynes
    25. Priyamvada Acharya

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  2. Converting non-neutralizing SARS-CoV-2 antibodies targeting conserved epitopes into broad-spectrum inhibitors through receptor blockade

    This article has 6 authors:
    1. Payton A.-B. Weidenbacher
    2. Eric Waltari
    3. Izumi de los Rios Kobara
    4. Benjamin N. Bell
    5. John E. Pak
    6. Peter S. Kim

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  3. Structural changes in the SARS-CoV-2 spike E406W mutant escaping a clinical monoclonal antibody cocktail

    This article has 12 authors:
    1. Amin Addetia
    2. Young-Jun Park
    3. Tyler Starr
    4. Allison J. Greaney
    5. Kaitlin R. Sprouse
    6. John E. Bowen
    7. Sasha W. Tiles
    8. Wesley C. Van Voorhis
    9. Jesse D. Bloom
    10. Davide Corti
    11. Alexandra C. Walls
    12. David Veesler

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  4. Molecular architecture of the human tRNA ligase complex

    This article has 11 authors:
    1. Alena Kroupova
    2. Fabian Ackle
    3. Igor Asanović
    4. Stefan Weitzer
    5. Franziska M Boneberg
    6. Marco Faini
    7. Alexander Leitner
    8. Alessia Chui
    9. Ruedi Aebersold
    10. Javier Martinez
    11. Martin Jinek
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This manuscript describes a tour-de-force approach to determine how the five subunits of the human tRNA ligase complex interact with each other. The authors combine X-ray crystallography of individual protein domains with combinatorial reconstitution analysis and cross-linking mass spectrometry to define and purify a minimal tRNA ligase complex core. Their reductionist and highly analytical approach produces a treasure-trove of data with exceptional quality, the true value of which will become apparent once additional structural information (from e.g. cryo-electron microscopy) becomes available, enabling independent biochemical validation.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  5. Creation of photocyclic vertebrate rhodopsin by single amino acid substitution

    This article has 4 authors:
    1. Kazumi Sakai
    2. Yoshinori Shichida
    3. Yasushi Imamoto
    4. Takahiro Yamashita
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This manuscript describes an investigation of the evolution of monostable rhodopsins, typically found in vertebrates. It highlights that single amino acid changes in vertebrate rhodopsins can create a partial bistable retinal pigment that can be photoconverted back to the ground state or it will slowly convert back to the ground state retinal isomer. The rationale for the experiments came from the discovery of a very interesting activation mechanism of the nonvisual pigment Opn5L1. This work has important implications for how our visual pigments have been optimized during evolution, and it contributes important insights into engineering bistable pigments for optogenetic applications.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. Dithiothreitol causes toxicity in C. elegans by modulating the methionine–homocysteine cycle

    This article has 2 authors:
    1. Gokul G
    2. Jogender Singh
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      Thiol agents, such as dithiothreitol (DTT), are toxic to many species, but the mechanisms of toxicity is incompletely understood. In this work, the authors use the animal C. elegans, a small worm, to propose a new mechanisms for how DTT causes organismal growth arrest. Specifically, they suggest that DTT causes reduction in the key molecule S-adenosyl methionine (SAM), which is used as a methyl donor to modify proteins, lipid, and/or other macromolecules. The genetic and supplementation experiments by the authors are compelling, but no direct evidence is provided that SAM levels are indeed lower following exposure of C. elegans to DTT.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  7. Structural basis for the in vitro efficacy of nirmatrelvir against SARS-CoV-2 variants

    This article has 12 authors:
    1. Samantha E. Greasley
    2. Stephen Noell
    3. Olga Plotnikova
    4. RoseAnn Ferre
    5. Wei Liu
    6. Ben Bolanos
    7. Kimberly Fennell
    8. Jennifer Nicki
    9. Tim Craig
    10. Yuao Zhu
    11. Al E. Stewart
    12. Claire M. Steppan

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  8. Glypican-1 drives unconventional secretion of fibroblast growth factor 2

    This article has 9 authors:
    1. Carola Sparn
    2. Eleni Dimou
    3. Annalena Meyer
    4. Roberto Saleppico
    5. Sabine Wegehingel
    6. Matthias Gerstner
    7. Severina Klaus
    8. Helge Ewers
    9. Walter Nickel
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      FGF2 moves directly from the cytoplasm through the plasma membrane in a reaction driven by its subsequent high affinity binding to cell surface heparan sulfate proteoglycans. This study concludes that Glypican-1 is the principal proteoglycan involved, possibly involving a unique tri-sulfated disaccharide binding site in close proximity to the cell surface. While the role of Glypican-1 appears unique to FGF2 rather than part of a generalized direct secretion mechanism, the observations highlight the complexity and significance of proteoglycan variation. The work is well done and generally convincing, but additional support for the authors' conclusion that a specific glycan structure in GPC1 is a specific ligand for FGF2 is required.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  9. Structural and functional impact by SARS-CoV-2 Omicron spike mutations

    This article has 18 authors:
    1. Jun Zhang
    2. Yongfei Cai
    3. Christy L. Lavine
    4. Hanqin Peng
    5. Haisun Zhu
    6. Krishna Anand
    7. Pei Tong
    8. Avneesh Gautam
    9. Megan L. Mayer
    10. Sophia Rits-Volloch
    11. Shaowei Wang
    12. Piotr Sliz
    13. Duane R. Wesemann
    14. Wei Yang
    15. Michael S. Seaman
    16. Jianming Lu
    17. Tianshu Xiao
    18. Bing Chen

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  10. Molecular basis of SARS-CoV-2 Omicron variant receptor engagement and antibody evasion and neutralization

    This article has 10 authors:
    1. Qin Hong
    2. Wenyu Han
    3. Jiawei Li
    4. Shiqi Xu
    5. Yifan Wang
    6. Zuyang Li
    7. Yanxing Wang
    8. Chao Zhang
    9. Zhong Huang
    10. Yao Cong

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    This article has 1 evaluationAppears in 1 listLatest version Latest activity
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