Biochemistry

Target binding triggers hierarchical phosphorylation of human Argonaute-2 to promote target release

1. Brianna Bibel
2. Elad Elkayam
3. Steve Silletti
4. Elizabeth A Komives
5. Leemor Joshua-Tor

• Curated by eLife

  Evaluation Summary:

  This paper provides well documented and solid biochemical data to show how phosphorylation of AGO2 modulate its binding to target mRNAs, releasing the complex to allow its recycling in the cell via electrostatic repulsion. This result could explain how a small amount of Ago proteins could target a very large number of mRNA molecules . The data support the key claims of the manuscript, and the approaches used are rigorous. This very well-written and elegant study will be of great interest to those working in the miRNA field as it addresses important open questions concerning the dynamic regulation of mIRNA-mediated gene repression.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Redox regulation of the SARS-CoV-2 main protease provides new opportunities for drug design

1. Lisa-Marie Funk
2. Gereon Poschmann
3. Ashwin Chari
4. Fabian Rabe von Pappenheim
5. Kim-Maren Stegmann
6. Antje Dickmanns
7. Nora Eulig
8. Marie Wensien
9. Elham Paknia
10. Gabi Heyne
11. Elke Penka
12. Arwen R. Pearson
13. Carsten Berndt
14. Tobias Fritz
15. Sophia Bazzi
16. Jon Uranga
17. Ricardo A. Mata
18. Matthias Dobbelstein
19. Rolf Hilgenfeld
20. Ute Curth
21. Kai Tittmann

Reviewed by ScreenIT

Uncovering the structural flexibility of SARS-CoV-2 glycoprotein spike variants

1. Hiam R. S. Arruda
2. Tulio M. Lima
3. Renata G. F. Alvim
4. Fernanda B. A. Victorio
5. Daniel P. B. Abreu
6. Federico F. Marsili
7. Karen D. Cruz
8. Patricia Sosa-Acosta
9. Mauricio Quinones-Vega
10. Jéssica de S. Guedes
11. Fábio C. S. Nogueira
12. Jerson L. Silva
13. Leda R. Castilho
14. Guilherme A. P. de Oliveira

Reviewed by ScreenIT

A potent synthetic nanobody with broad-spectrum activity neutralizes SARS-Cov-2 virus and Omicron variant through a unique binding mode

1. Dongping Zhao
2. Liqin Liu
3. Xinlin Liu
4. Jinlei Zhang
5. Yuqing Yin
6. Linli Luan
7. Dingwen Jiang
8. Xiong Yang
9. Lei Li
10. Hualong Xiong
11. Dongming Xing
12. Qingbing Zheng
13. Ningshao Xia
14. Yuyong Tao
15. Shaowei Li
16. Haiming Huang

Reviewed by ScreenIT

Structure-based electron-confurcation mechanism of the Ldh-EtfAB complex

1. Kanwal Kayastha
2. Alexander Katsyv
3. Christina Himmrich
4. Sonja Welsch
5. Jan M Schuller
6. Ulrich Ermler
7. Volker Müller

• Curated by eLife

  Evaluation Summary:

  This paper describes a new structure for a complex between a bifurcating electron transfer flavoprotein (ETF) and its client dehydrogenase. Because electrons are being supplied by the dehydrogenase, the ETF executes confurcation in contrast to all of those elucidated so far, which function in the opposite direction to effect bifurcation. As electron-confurcation and electron-bifurcation have emerged as important paradigms of cellular bioenergetics, the data reported herein pave the way for future exploration of similar electron transfer systems and lay the ground for understanding their structural basis. The work will be of relevance to all who are interested in the mechanisms of enzymes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike

1. Victoria Stalls
2. Jared Lindenberger
3. Sophie M.-C. Gobeil
4. Rory Henderson
5. Rob Parks
6. Maggie Barr
7. Margaret Deyton
8. Mitchell Martin
9. Katarzyna Janowska
10. Xiao Huang
11. Aaron May
12. Micah Speakman
13. Esther Beaudoin
14. Bryan Kraft
15. Xiaozhi Lu
16. Robert J. Edwards
17. Amanda Eaton
18. David C. Montefiori
19. Wilton B. Williams
20. Kevin O. Saunders
21. Kevin Wiehe
22. Barton F. Haynes
23. Priyamvada Acharya

Reviewed by ScreenIT

Batch Production of High-Quality Graphene Grids for Cryo-EM: Cryo-EM Structure of Methylococcus capsulatus Soluble Methane Monooxygenase Hydroxylase

1. Eungjin Ahn
2. Byungchul Kim
3. Soyoung Park
4. Amanda L. Erwin
5. Suk Hyun Sung
6. Robert Hovden
7. Shyamal Mosalaganti
8. Uhn-Soo Cho

• Curated by eLife

  Evaluation Summary:

  In this work, the authors describe a protocol for coating supports for transmission electron microscopy with graphene. The approach uses a commercially available PMMA/graphene pad and the authors provide a new process for PMMA removal after graphene transfer. This work has the potential to be an important methodological step forward - if a way were found to remove amorphous residue from the surface of graphene. The work has the potential to be of broad interest to the many structural biologists using cryo-EM techniques.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Rate-limiting transport of positively charged arginine residues through the Sec-machinery is integral to the mechanism of protein secretion

1. William J Allen
2. Robin A Corey
3. Daniel W Watkins
4. A Sofia F Oliveira
5. Kiel Hards
6. Gregory M Cook
7. Ian Collinson

• Curated by eLife

  Evaluation Summary:

  Here, using a novel bioluminescence-based assay, authors dissect the sequence features of client proteins that influence SecA/SecYEG-mediated protein translocation across the bacterial inner membranes. Combined with rigorous kinetic modeling, this study pushes the description of this important cellular pathway towards a highly detailed level, which will potentially advance our understanding of ATP-driven protein secretion mechanisms in bacteria. The main conclusions are well supported, and the paper will be interesting to both those in the field of protein transport and to a broader audience.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

A network of cytosolic (co)chaperones promotes the biogenesis of mitochondrial signal-anchored outer membrane proteins

1. Layla Drwesh
2. Benjamin Heim
3. Max Graf
4. Linda Kehr
5. Lea Hansen-Palmus
6. Mirita Franz-Wachtel
7. Boris Macek
8. Hubert Kalbacher
9. Johannes Buchner
10. Doron Rapaport

• Curated by eLife

  Evaluation Summary:

  The authors dissect and reconstitute the cytosolic steps for mitochondrial signal-anchored membrane protein biogenesis focusing on post-translational precursor recognition by cytosolic chaperones and their subsequent transfer to import receptors located within the mitochondrial outer membrane. These are crucial events in order to assist proper protein biogenesis while preventing aggregation and its downstream consequences. The study is an important contribution to the understanding of cytosolic events in the biogenesis of mitochondrial proteins, and this paper will be of relevance for researchers in the fields of chaperone and mitochondrial biology as well as for cell biologists studying the biogenesis of membrane proteins.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Changes of urinary proteomic before and after QIV and COVID-19 vaccination

1. Xuanzhen Pan
2. Yongtao Liu
3. Yijin Bao
4. Lilong Wei
5. Youhe Gao

Reviewed by ScreenIT