Biochemistry

Structural insights into the GTP-driven monomerization and activation of a bacterial LRRK2 homolog using allosteric nanobodies

1. Christian Galicia
2. Giambattista Guaitoli
3. Marcus Fislage
4. Christian Johannes Gloeckner
5. Wim Versées

• Curated by eLife

  eLife assessment

  The fundamental study by Galicia C. et al. captured the GTP-bound active structure of CtRoco, a homolog of human LRRK2, using conformation-specific nanobodies. This convincing body of work reports the first structure of a GTP-bound ROCO protein, illustrating how GTP facilitates the dimer-to-monomer transition of CtRoco and functional activation.

Reviewed by eLife

A multi-hierarchical approach reveals d-serine as a hidden substrate of sodium-coupled monocarboxylate transporters

1. Pattama Wiriyasermkul
2. Satomi Moriyama
3. Masataka Suzuki
4. Pornparn Kongpracha
5. Nodoka Nakamae
6. Saki Takeshita
7. Yoko Tanaka
8. Akina Matsuda
9. Masaki Miyasaka
10. Kenji Hamase
11. Tomonori Kimura
12. Masashi Mita
13. Jumpei Sasabe
14. Shushi Nagamori

• Curated by eLife

  eLife assessment

  This study shows compelling evidence that the less common D-serine stereoisomer is transported in the kidney by the neutral amino acid transporter ASCT2 and that it is a non-canonical substrate for sodium-coupled monocarboxylate transporter SMCTs. With a multi-hierarchical approach, this important study further shows that Ischemia-Reperfusion Injury in the kidney causes a specific increment in renal reabsorption carried out, in part, by ASCT2.

Reviewed by eLife

Identification of an evolutionary conserved binding motif responsible for the recruitment of NMD factors to the UPF1 helicase

1. Irène Barbarin-Bocahu
2. Nathalie Ulryck
3. Amandine Rigobert
4. Nadia Ruiz Gutierrez
5. Laurence Decourty
6. Mouna Raji
7. Bhumika Garkhal
8. Hervé Le Hir
9. Cosmin Saveanu
10. Marc Graille

Reviewed by Review Commons

Caenorhabditis elegans Dicer acts with the RIG-I-like helicase DRH-1 and RDE-4 to cleave dsRNA

1. Claudia D Consalvo
2. Adedeji M Aderounmu
3. Helen M Donelick
4. P Joseph Aruscavage
5. Debra M Eckert
6. Peter S Shen
7. Brenda L Bass

• Curated by eLife

  eLife assessment

  To investigate the evolutionary relationship between the RNAi pathway and innate immunity, this valuable study uses biochemistry and structural biology to investigate the trimeric complex of Dicer-1, DRH-1 (a RIGI homologue), and RDE-4, which exists in C. elegans. The results described include rigorous kinetic analysis of the enzymatic activity of the complex and a moderate resolution cryo-EM structure. The results are convincing and add to the broader understanding of the evolution of antiviral defense.

Reviewed by eLife

Giardia intestinalis deoxyadenosine kinase has a unique tetrameric structure that enables high substrate affinity and makes the parasite sensitive to deoxyadenosine analogues

1. Farahnaz Ranjbarian
2. Karim Rafie
3. Kasturika Shankar
4. Sascha Krakovka
5. Staffan G. Svärd
6. Lars-Anders Carlson
7. Anders Hofer

Reviewed by Review Commons

CK-666 and CK-869 differentially inhibit Arp2/3 iso-complexes

1. LuYan Cao
2. Shaina Huang
3. Angika Basant
4. Miroslav Mladenov
5. Michael Way

Reviewed by Review Commons

Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system

1. Fujun Zhou
2. Julie M Bocetti
3. Meizhen Hou
4. Daoming Qin
5. Alan G Hinnebusch
6. Jon R Lorsch

• Curated by eLife

  eLife assessment

  This is an important paper as it is the first to use a reconstituted translation system to study competition among mRNAs for the initiation machinery. Understanding the principles of the biochemistry of mRNA competition for initiation factors cannot be achieved without such a system. The authors provide compelling evidence that Ded1 is required for efficient initiation of highly structured mRNAs. The findings are significant and validate the in vitro reconstituted system by recapitulating the effects of in-vivo perturbations of translation initiation by Ded1 mutants.

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Effects of phosphorylation on Drp1 activation by its receptors, actin, and cardiolipin

1. Ao Liu
2. Anna L. Hatch
3. Henry N. Higgs

Reviewed by Review Commons

Fascin-induced bundling protects actin filaments from disassembly by cofilin

1. Jahnavi Chikireddy
2. Léana Lengagne
3. Rémi Le Borgne
4. Catherine Durieu
5. Hugo Wioland
6. Guillaume Romet-Lemonne
7. Antoine Jégou

Reviewed by Review Commons

The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase

1. Valentin Bohl
2. Nele Merret Hollmann
3. Tobias Melzer
4. Panagiotis Katikaridis
5. Lena Meins
6. Bernd Simon
7. Dirk Flemming
8. Irmgard Sinning
9. Janosch Hennig
10. Axel Mogk

• Curated by eLife

  eLife assessment

  This important manuscript details the characterization of ClpL from L. monocytogenes as an effective and autonomous AAA+ disaggregase that provides enhanced heat resistance to this food-borne pathogen. Supported by compelling evidence, the authors demonstrate that ClpL has DnaK-independent disaggregase activity towards a variety of aggregated model substrates, which is more potent than that observed with the endogenous canonical DnaK/ClpB bi-chaperone system. The work will be of broad interest to microbiologists and biochemists.

Reviewed by eLife