Biochemistry

  4. Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic Regions

    1. Heather J Ellis
    2. Matthew Chan
    3. Balaji Selvam
    4. Evan Walter
    5. Christine A Devlin
    6. Steven K Szymanski
    7. Loren Keith Henry
    8. Diwakar Shukla
    9. Erik Procko

    • Curated by eLife

      eLife assessment

      Serotonin is an important neurotransmitter and its synaptic concentration is controlled by re-uptake by the sodium-coupled serotonin transporter SERT. The manuscript by Chan et al reports results from a systematic deep mutagenesis approach to study the surface expression and APP+ (5HT analogue) transport mechanism of the human serotonin transporter. The authors complement this experimental evidence with large-scale molecular simulations of the transporter in the presence of APP+. The use of deep mutagenesis and large-scale adaptive sampling simulations is impressive, and could contribute to understanding the structural requirements for folding and how transporters evolve to recognize different substrates.

    Reviewed by eLife

    5 evaluationsAppears in 2 listsLatest version Latest activity

  7. Mapping protein-metabolite interactions in E. coli by integrating chromatographic techniques and co-fractionation mass spectrometry

    1. Mateusz Wagner
    2. Jieun Kang
    3. Catherine Mercado
    4. Venkatesh P. Thirumalaikumar
    5. Michal Gorka
    6. Hanne Zillmer
    7. Jingzhe Guo
    8. Romina I. Minen
    9. Caroline F. Plecki
    10. Katayoon Dehesh
    11. Frank C. Schroeder
    12. Dirk Walther
    13. Aleksandra Skirycz

    Reviewed by Review Commons

    4 evaluationsAppears in 1 listLatest version Latest activity
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