Molecular Insights into Single-Chain Lipid Modulation of Acid-Sensing Ion Channel 3
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Abstract
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We repeated these experiments after preincubating our cells in 10μM DHA for 10 minutes
Is 10uM near the physiologic concentration for docosahexaenoic acid?
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Overall, the presence of DHA, whether docked to the TM helices of hASIC3 or partitioned into the lipid layer, prevented POPC lipids from accessing and penetrating the pore, ensuring high permeability for water and Na+ ions to flow through the channel.
Based on your model, can you postulate different lipid structures or lipid classes that could alter pore function?
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Based on this experimental data, we designed our PUFA-bound simulations where we docked either AG
This is a very interesting study! Using your model, can you predict other channel-specific amino acids that are important for binding to lipid mediators? If so, it would be interesting to mutate these residues and show they have a similar effect as R63 mutants.
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