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  1. Domain Coupling in Allosteric Regulation of SthK Measured Using Time-Resolved Transition Metal Ion FRET

    This article has 3 authors:
    1. Pierce Eggan
    2. Sharona E Gordon
    3. William N Zagotta
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This useful work employs transition-metal FRET (tmFRET) to study the cyclic nucleotide binding domain (CNBD) of a bacterial ion channel. The authors employ lifetime measurements of fluorescence to extend their own prior study and observe distance changes within the CNBD domains of a full-length channel; they base these measurements on changes in lifetimes due to tmFRET between a metal at an introduced chelator site and a fluorescent non-canonical amino acid at another site within the channel sequence. This allows the authors to show that coupling of the CNBDs to the rest of the channel stabilizes the CNBDs in their active state relative to an isolated CNBD construct. The data are compelling and of high quality, and support the authors' conclusions.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  2. Molecular basis for multidrug efflux by an anaerobic RND transporter

    This article has 16 authors:
    1. Ryan Lawrence
    2. Mohd Athar
    3. Muhammad R. Uddin
    4. Christopher Adams
    5. Joana S. Sousa
    6. Oliver Durrant
    7. Sophie Lellman
    8. Lucy Sutton
    9. C. William Keevil
    10. Nisha Patel
    11. Christine Prosser
    12. David McMillan
    13. Helen I. Zgurskaya
    14. Attilio V. Vargiu
    15. Zainab Ahdash
    16. Eamonn Reading
    This article has no evaluationsAppears in 1 listLatest version
  3. De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors

    This article has 46 authors:
    1. Edin Muratspahić
    2. David Feldman
    3. David E. Kim
    4. Xiangli Qu
    5. Ana-Maria Bratovianu
    6. Paula Rivera-Sánchez
    7. Federica Dimitri
    8. Jason Cao
    9. Brian P. Cary
    10. Matthew J. Belousoff
    11. Peter Keov
    12. Qingchao Chen
    13. Yue Ren
    14. Justyn Fine
    15. Isaac Sappington
    16. Thomas Schlichthaerle
    17. Jason Z. Zhang
    18. Arvind Pillai
    19. Ljubica Mihaljević
    20. Magnus Bauer
    21. Susana Vázquez Torres
    22. Amir Motmaen
    23. Gyu Rie Lee
    24. Long Tran
    25. Xinru Wang
    26. Inna Goreshnik
    27. Dionne K. Vafeados
    28. Justin E. Svendsen
    29. Parisa Hosseinzadeh
    30. Nicolai Lindegaard
    31. Matthäus Brandt
    32. Yann WaltenspĂĽhl
    33. Kristine Deibler
    34. Luke Oostdyk
    35. William Cao
    36. Lakshmi Anantharaman
    37. Lance Stewart
    38. Lauren Halloran
    39. Jamie B. Spangler
    40. Patrick M. Sexton
    41. Bryan L. Roth
    42. Brian E. Krumm
    43. Denise Wootten
    44. Christopher G. Tate
    45. Christoffer Norn
    46. David Baker
    This article has no evaluationsAppears in 1 listLatest version